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1.发明授权Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials 有权包含对非纤维素材料具有降低的亲和力的纤维素酶变体的组合物和方法公开(公告)号：US08236542B2
公开(公告)日：2012-08-07
申请号：US12477887
申请日：2009-06-03
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.
摘要翻译： 本公开涉及纤维素酶变体。 特别地，本公开涉及具有减少的与非纤维素材料结合的纤维素酶变体。 还描述了编码纤维素酶的核酸，包含所述纤维素酶的组合物，鉴定纤维素变体的方法和使用该组合物的方法。
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2.发明授权Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials 有权包含对非纤维素材料具有降低的亲和力的纤维素酶变体的组合物和方法公开(公告)号：US08999692B2
公开(公告)日：2015-04-07
申请号：US13543627
申请日：2012-07-06
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.
摘要翻译： 本公开涉及纤维素酶变体。 特别地，本公开涉及具有减少的与非纤维素材料结合的纤维素酶变体。 还描述了编码纤维素酶的核酸，包含所述纤维素酶的组合物，鉴定纤维素变体的方法和使用该组合物的方法。
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3.发明申请COMPOSITIONS AND METHODS COMPRISING CELLULASE VARIANTS WITH REDUCED AFFINITY TO NON-CELLULOSIC MATERIALS 有权包含具有减少对非细胞材料的亲和力的纤维素酶变体的组合物和方法公开(公告)号：US20120276595A1
公开(公告)日：2012-11-01
申请号：US13543627
申请日：2012-07-06
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.
摘要翻译： 本公开涉及纤维素酶变体。 特别地，本公开涉及具有减少的与非纤维素材料结合的纤维素酶变体。 还描述了编码纤维素酶的核酸，包含所述纤维素酶的组合物，鉴定纤维素变体的方法和使用该组合物的方法。
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4.发明申请Compositions and Methods Comprising Cellulase Variants with Reduced Affinity to Non-Cellulosic Materials 有权包含与非纤维素材料亲和力降低的纤维素酶变体的组合物和方法公开(公告)号：US20100041104A1
公开(公告)日：2010-02-18
申请号：US12477887
申请日：2009-06-03
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.
摘要翻译： 本公开涉及纤维素酶变体。 特别地，本公开涉及具有减少的与非纤维素材料结合的纤维素酶变体。 还描述了编码纤维素酶的核酸，包含所述纤维素酶的组合物，鉴定纤维素变体的方法和使用该组合物的方法。
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公开(公告)号：US08569034B2
公开(公告)日：2013-10-29
申请号：US12740782
申请日：2008-10-28
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38618 , C11D3/38636 , C12Y304/24027
摘要： The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.
摘要翻译： 本发明提供包含至少一种具有改善的储存稳定性和/或催化活性的嗜热菌蛋白酶样中性蛋白酶的方法和组合物。 在一些实施方案中，嗜热菌蛋白酶用于清洁和其它包含洗涤剂的应用。 在一些特别优选的实施方案中，本发明提供包含热溶菌素的方法和组合物，其配制和/或工程化以抵抗洗涤剂诱导的失活。
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公开(公告)号：US20110081454A1
公开(公告)日：2011-04-07
申请号：US12681614
申请日：2008-10-23
申请人： Ronaldus W. J. Hommes , Amy D. Liu , Andrew Shaw , Louise Wallace
发明人： Ronaldus W. J. Hommes , Amy D. Liu , Andrew Shaw , Louise Wallace
IPC分类号： C12N9/50 , C12N9/54 , C07H21/00 , C12N15/63 , C12N1/21 , C12N9/26 , C12N9/20 , C12N9/42 , C12N9/24 , C12N9/18 , C12N9/02 , D06M16/00 , A23K1/165
CPC分类号： C11D3/38618 , C12N9/54
摘要： The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved stability in the presence of a metal chelator. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications comprising citrate. In some particularly preferred embodiments, the present invention provides methods and compositions comprising variant neutral metalloprotease(s) engineered to resist citrate-induced autolysis.
摘要翻译： 本发明提供包含至少一种在金属螯合剂存在下具有改善的稳定性的中性金属蛋白酶的方法和组合物。 在一些实施方案中，中性金属蛋白酶可用于清洁和其它包含柠檬酸盐的应用。 在一些特别优选的实施方案中，本发明提供了包含改变为抗柠檬酸盐诱导的自溶的变体中性金属蛋白酶的方法和组合物。
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公开(公告)号：US07125695B2
公开(公告)日：2006-10-24
申请号：US10719571
申请日：2003-11-20
CPC分类号： C12N9/0006 , C07K14/245 , C12N15/102 , C12P7/18 , C12R1/01 , C12R1/19
摘要： The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.
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公开(公告)号：US06706503B2
公开(公告)日：2004-03-16
申请号：US10037677
申请日：2001-10-23
IPC分类号： C12P104
CPC分类号： C12N9/0006 , C07K14/245 , C12N15/102 , C12P7/18 , C12R1/01 , C12R1/19
摘要： The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.
摘要翻译： 本发明提供了用于引导微生物进化的方法，所述微生物包括使用突变基因并在选择压力条件下生长。 该方法公开了可用于本发明方法的突变基因，并提供了例证通过该方法产生的进化微生物的ATCC沉积物。
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公开(公告)号：US06365410B1
公开(公告)日：2002-04-02
申请号：US09314847
申请日：1999-05-19
IPC分类号： C12N1574
CPC分类号： C12N9/0006 , C07K14/245 , C12N15/102 , C12P7/18 , C12R1/01 , C12R1/19
摘要： The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.
摘要翻译： 本发明提供了用于引导微生物进化的方法，所述微生物包括使用突变基因并在选择压力条件下生长。 该方法公开了可用于本发明方法的突变基因，并提供了例证通过该方法产生的进化微生物的ATCC沉积物。
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10.发明申请公开(公告)号：US20120009651A1
公开(公告)日：2012-01-12
申请号：US12740782
申请日：2008-10-28
申请人： David A. Estell , Ronaldus W.J. Hommes , Amy D. Liu , Andrew Shaw
发明人： David A. Estell , Ronaldus W.J. Hommes , Amy D. Liu , Andrew Shaw
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38618 , C11D3/38636 , C12Y304/24027
摘要： The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation.
摘要翻译： 本发明提供包含至少一种具有改善的储存稳定性和/或催化活性的嗜热菌蛋白酶样中性蛋白酶的方法和组合物。 在一些实施方案中，嗜热菌蛋白酶用于清洁和其它包含洗涤剂的应用。 在一些特别优选的实施方案中，本发明提供包含热溶菌素的方法和组合物，其配制和/或工程化以抵抗洗涤剂诱导的失活。
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