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1.发明授权Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials 有权包含对非纤维素材料具有降低的亲和力的纤维素酶变体的组合物和方法公开(公告)号：US08236542B2
公开(公告)日：2012-08-07
申请号：US12477887
申请日：2009-06-03
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.
摘要翻译： 本公开涉及纤维素酶变体。 特别地，本公开涉及具有减少的与非纤维素材料结合的纤维素酶变体。 还描述了编码纤维素酶的核酸，包含所述纤维素酶的组合物，鉴定纤维素变体的方法和使用该组合物的方法。
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2.发明授权Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials 有权包含对非纤维素材料具有降低的亲和力的纤维素酶变体的组合物和方法公开(公告)号：US08999692B2
公开(公告)日：2015-04-07
申请号：US13543627
申请日：2012-07-06
IPC分类号： C12N9/42
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.
摘要翻译： 本公开涉及纤维素酶变体。 特别地，本公开涉及具有减少的与非纤维素材料结合的纤维素酶变体。 还描述了编码纤维素酶的核酸，包含所述纤维素酶的组合物，鉴定纤维素变体的方法和使用该组合物的方法。
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3.发明申请COMPOSITIONS AND METHODS COMPRISING CELLULASE VARIANTS WITH REDUCED AFFINITY TO NON-CELLULOSIC MATERIALS 有权包含具有减少对非细胞材料的亲和力的纤维素酶变体的组合物和方法公开(公告)号：US20120276595A1
公开(公告)日：2012-11-01
申请号：US13543627
申请日：2012-07-06
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.
摘要翻译： 本公开涉及纤维素酶变体。 特别地，本公开涉及具有减少的与非纤维素材料结合的纤维素酶变体。 还描述了编码纤维素酶的核酸，包含所述纤维素酶的组合物，鉴定纤维素变体的方法和使用该组合物的方法。
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4.发明申请Compositions and Methods Comprising Cellulase Variants with Reduced Affinity to Non-Cellulosic Materials 有权包含与非纤维素材料亲和力降低的纤维素酶变体的组合物和方法公开(公告)号：US20100041104A1
公开(公告)日：2010-02-18
申请号：US12477887
申请日：2009-06-03
CPC分类号： C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01004 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having reduced binding to non-cellulosic materials. Also described are nucleic acids encoding the cellulase, compositions comprising said cellulase, methods of identifying cellulose variants and methods of using the compositions.
摘要翻译： 本公开涉及纤维素酶变体。 特别地，本公开涉及具有减少的与非纤维素材料结合的纤维素酶变体。 还描述了编码纤维素酶的核酸，包含所述纤维素酶的组合物，鉴定纤维素变体的方法和使用该组合物的方法。
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公开(公告)号：US20120003718A1
公开(公告)日：2012-01-05
申请号：US13128854
申请日：2009-11-10
IPC分类号： C12N9/54 , C12N15/75 , C12S11/00 , B08B3/00 , C11D3/60 , C12N1/21 , D06L1/16 , C12N15/57 , C12S9/00
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/38663 , C11D3/38681 , C12N15/52 , C12Y304/21062
摘要： The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.
摘要翻译： 本发明提供工程蛋白酶变体。 特别地，蛋白酶变体在选定的表面位置包含影响酶的电荷和/或疏水性的可组合突变，以在所选择的应用中增强所得变体酶的至少一种所需性质。 还提供了包含蛋白酶变体的组合物及其使用方法。
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6.发明申请Variant Alpha-Amylases from Bacillus Subtilis and Methods of Uses, Thereof 有权来自枯草芽孢杆菌的变体α-淀粉酶及其使用方法公开(公告)号：US20100015686A1
公开(公告)日：2010-01-21
申请号：US12479427
申请日：2009-06-05
申请人： Luis G. Cascao-Pereira , William A. Cuevas , David A. Estell , Sang-Kyu Lee , Scott D. Power , Sandra W. Ramer , Amr Toppozada , Louise Wallace
发明人： Luis G. Cascao-Pereira , William A. Cuevas , David A. Estell , Sang-Kyu Lee , Scott D. Power , Sandra W. Ramer , Amr Toppozada , Louise Wallace
IPC分类号： C12N9/28
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2408 , C12P7/14 , C12P19/02 , C12P19/14 , G01N11/00 , Y02E50/17
摘要： Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
摘要翻译： 提供了来自枯草芽孢杆菌（AmyE）的α-淀粉酶，其变体，编码它们的核酸和包含核酸的宿主细胞。 公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。 这样的方法可能产生可用于乙醇生产或高果糖玉米糖浆生产的糖。 在一些情况下，淀粉酶可以在低pH，不存在钙的情况下和/或不存在葡糖淀粉酶的情况下使用。
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公开(公告)号：US20090252828A1
公开(公告)日：2009-10-08
申请号：US12263886
申请日：2008-11-03
申请人： Luis G. Cascao-Pereira , James T. Kellis, JR. , Bradley A. Paulson , Scott D. Power , Sandra W. Ramer , Vivek Sharma , Andrew Shaw , Jayarama K. Shetty , Donald E. Ward
发明人： Luis G. Cascao-Pereira , James T. Kellis, JR. , Bradley A. Paulson , Scott D. Power , Sandra W. Ramer , Vivek Sharma , Andrew Shaw , Jayarama K. Shetty , Donald E. Ward
CPC分类号： C11D3/38681 , C11D3/386 , C12N9/2417 , D06M16/003
摘要： Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.
摘要翻译： 公开了包含α-淀粉酶变体的组合物，其具有α-淀粉酶活性，并且相对于其来源的亲本AmyS样α-淀粉酶表现出改变的性质。 组合物包含另外的酶如植酸酶。 还公开了使用组合物的方法和与其相关的试剂盒。
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公开(公告)号：US09856466B2
公开(公告)日：2018-01-02
申请号：US14115801
申请日：2012-05-04
申请人： Neelam S. Amin , Katherine Augustyn , Joshua R. Basler , Luis G. Cascao-Pereira , Katherine D. Collier , Edward M. Concar , David A. Estell , James T. Kellis, Jr. , Euan John Magennis , Alexander Pisarchik , Ayrookaran J. Poulose , Philip Frank Souter , Glenn Steven Ward , Jian Yao
发明人： Neelam S. Amin , Katherine Augustyn , Joshua R. Basler , Luis G. Cascao-Pereira , Katherine D. Collier , Edward M. Concar , David A. Estell , James T. Kellis, Jr. , Euan John Magennis , Alexander Pisarchik , Ayrookaran J. Poulose , Philip Frank Souter , Glenn Steven Ward , Jian Yao
摘要： The present invention provides serine protease variants, more specifically subtilisin variants produced there from. Specifically, the present invention provides serine protease variants, more specifically subtilisin variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants, more specifically subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants, more specifically subtilisin variants.
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公开(公告)号：US08236545B2
公开(公告)日：2012-08-07
申请号：US12365646
申请日：2009-02-04
申请人： Luis G. Cascao-Pereira , Claudine Chang , Clement Choy , James T. Kellis, Jr. , Brian E Jones , Melodie Estabrook , Marc Kolkman , Chris Leeflang , Casper Vroemen , Walter Weyler
发明人： Luis G. Cascao-Pereira , Claudine Chang , Clement Choy , James T. Kellis, Jr. , Brian E Jones , Melodie Estabrook , Marc Kolkman , Chris Leeflang , Casper Vroemen , Walter Weyler
CPC分类号： C12N9/2417 , A21D8/042 , A23K20/189 , A23K50/75 , A23L29/06
摘要： Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.
摘要翻译： 描述了具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质的亲本α-淀粉酶的变体（突变体）及其使用方法。
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公开(公告)号：US20100192985A1
公开(公告)日：2010-08-05
申请号：US12616097
申请日：2009-11-10
申请人： Wolfgang Aehle , Luis G. Cascao-Pereira , David A. Estell , Frits Goedegebuur , James T. Kellis, JR. , Ayrookaran J. Poulose , Brian F. Schmidt
发明人： Wolfgang Aehle , Luis G. Cascao-Pereira , David A. Estell , Frits Goedegebuur , James T. Kellis, JR. , Ayrookaran J. Poulose , Brian F. Schmidt
CPC分类号： C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/38636 , C11D3/38645 , C11D3/38654 , C12N9/50 , C12N9/54 , C12Y304/21062
摘要： The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.
摘要翻译： 本发明提供蛋白质工程方法和丝氨酸蛋白酶变体。 具体地，本发明提供了与参考丝氨酸蛋白酶相比具有一个或多个取代的丝氨酸蛋白酶变体。 此外，本发明提供包含这些丝氨酸蛋白酶变体的组合物。 在一些实施方案中，本发明提供了包含这些丝氨酸蛋白酶变体中的至少一种的清洁组合物。
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