公开(公告)号：US11039908B2

公开(公告)日：2021-06-22

申请号：US15737268

申请日：2016-06-15

申请人： MIE UNIVERSITY ,  DAICEL CORPORATION

发明人： Hiroshi Shiku ,  Yuzo Yamamoto ,  Takaya Masumoto

IPC分类号： A61D1/02 ,  A61D7/00 ,  A61K39/00 ,  A61K31/7088 ,  A61M5/20 ,  A61M5/30 ,  A61K9/00

摘要： A needleless injector which can selectively induce the immune response and a method for introducing DNA including a region coding for an antigen to selectively produce an antibody in a living body of a mammal by using the needleless injector are provided. Disclosed is a needleless injector for injecting a DNA solution into an injection target area without using any injection needles. The needleless injector includes an accommodating unit which accommodates the DNA solution, a predetermined ignition device; and a predetermined nozzle unit. A temperature of the predetermined combustion product, which is provided during the pressurization, changes to a neighborhood of ordinary temperature within 20 msec after a pressure, which is applied to the DNA solution on account of the combustion of the igniter powder, reaches an initial peak discharge force during a pressurization process for discharging the DNA solution.

公开(公告)号：US11497768B2

公开(公告)日：2022-11-15

申请号：US16619212

申请日：2018-06-05

申请人： MIE UNIVERSITY

发明人： Hiroshi Shiku ,  Yasushi Akahori ,  Yuya Kato ,  Yoshihiro Miyahara

IPC分类号： A61K35/17 ,  C07K14/725 ,  C07K14/705 ,  C07K16/28 ,  C12N15/63 ,  A61K39/00

摘要： CAR-T cells for cancer therapy are provided with an antibody that recognizes the MAGE-A4-derived-peptide/HLA-A2 complex. The antibody includes the VH amino acid sequence of SEQ ID NO: 36 and the VL amino acid sequence of SEQ ID NO: 38. The antibody preferably is provided with the amino acid sequence of SEQ ID NO: 32. Such CAR-T cells can be used in CAR infusion therapy in which a cancer-specific intracellular antigen is used.

公开(公告)号：US11179450B2

公开(公告)日：2021-11-23

申请号：US16035922

申请日：2018-07-16

申请人： MIE UNIVERSITY ,  KYOTO UNIVERSITY

发明人： Hiroshi Shiku ,  Naozumi Harada ,  Daisuke Muraoka ,  Kazunari Akiyoshi

IPC分类号： A61K39/00 ,  A61K39/015 ,  A61K39/12 ,  A61K47/36 ,  A61K9/00

摘要： A long-chain peptide antigen includes a plurality of epitopes. An interepitope sequence located between two of the plurality of epitopes contains four to ten consecutive tyrosines. The long-chain peptide antigen may be administered to a patient together with a hydrophobized polysaccharide, such as cholesterol-modified pullulan, and/or an adjuvant, such as CpG oligo DNA.

公开(公告)号：US20210239664A1

公开(公告)日：2021-08-05

申请号：US17263080

申请日：2019-07-31

申请人： Mie University

发明人： Hiroshi Shiku ,  Naohiro Seo ,  Tsuguhiro Kaneda ,  Junko Nakamura ,  Kazunari Akiyoshi

IPC分类号： G01N30/88 ,  G01N1/40

摘要： The present invention aims to provide a method for preparing exosomes comprising: (i) a step for ultrafiltering a sample containing at least one exosome; and (ii) a step for subjecting the sample that can be obtained from step (i) to anion exchange column chromatography.

公开(公告)号：US20210161963A1

公开(公告)日：2021-06-03

申请号：US17262900

申请日：2019-07-31

申请人： Mie University

发明人： Hiroshi Shiku ,  Yoshihiro Miyahara ,  Satoshi Okumura ,  Takuma Kato ,  Tae Hayashi ,  Yoshimasa Tanaka

IPC分类号： A61K35/17 ,  C07K14/725 ,  C12N5/0783 ,  C12N15/85

摘要： The present invention provides a method for efficiently producing highly pure γδT cells introduced with foreign genes by culturing in the presence of IL-7 and IL-15 after stimulating the γδT cells with a bisphosphonic acid ester derivative. When a T cell receptor (TCR) or a chimeric antigen receptor (CAR) is introduced as a foreign gene, γδT cells in which TCR and CAR are functionally expressed can be obtained.

公开(公告)号：US11952423B2

公开(公告)日：2024-04-09

申请号：US18318610

申请日：2023-05-16

申请人： MIE UNIVERSITY ,  DAIICHI SANKYO COMPANY, LIMITED

发明人： Hiroshi Shiku ,  Yasushi Akahori ,  Kento Tanaka ,  Ayaka Yatsu ,  Junya Ichikawa ,  Toshiaki Ohtsuka ,  Shiho Kozuma ,  Ryuji Hashimoto ,  Makiko Nakayama ,  Naoya Shinozaki ,  Kensuke Nakamura ,  Ichiro Watanabe ,  Shinji Furuzono

IPC分类号： C07K16/28 ,  C07K16/32

CPC分类号： C07K16/2809 ,  C07K16/32 ,  C07K2317/31 ,  C07K2317/52 ,  C07K2317/522 ,  C07K2317/524 ,  C07K2317/526 ,  C07K2317/528 ,  C07K2317/53 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/622

摘要： A novel antibody that can be used as an anti-tumor agent and an anti-tumor agent that comprises, as an active ingredient, a molecule containing such an antibody.

公开(公告)号：US09144603B2

公开(公告)日：2015-09-29

申请号：US14053997

申请日：2013-10-15

申请人： MIE UNIVERSITY ,  TAKARA BIO INC.

发明人： Hiroshi Shiku ,  Hiroaki Ikeda ,  Jun Mitsui ,  Yuki Takenaka ,  Junichi Mineno ,  Ikunoshin Kato

IPC分类号： A61K48/00 ,  A61K39/00 ,  A61K38/19 ,  C07K14/705 ,  A61K35/12 ,  A61K38/00

CPC分类号： A61K39/0005 ,  A61K35/12 ,  A61K38/00 ,  A61K38/191 ,  A61K39/0011 ,  A61K2039/5152 ,  A61K2039/5156 ,  C07K14/70575 ,  C07K2319/30 ,  C12N2799/022 ,  C12N2799/027

摘要： Disclosed are: a cell capable of expressing an exogenous GITRL or an exogenous GITRL derivative; a method for producing the cell; a therapeutic or prophylactic agent comprising the cell as an active ingredient; use of the cell in the manufacture of a therapeutic or prophylactic agent; a method comprising a step of administering the cell to a subject; a viral vector carrying a gene encoding a GITRL or a GITRL derivative; a therapeutic or prophylactic agent comprising the viral vector as an active ingredient; use of the viral vector in the manufacture of a therapeutic or prophylactic agent; and a method comprising a step of administering the viral vector to a subject.

摘要翻译： 公开了能够表达外源GITRL或外源性GITRL衍生物的细胞; 电池的制造方法; 包含该细胞作为活性成分的治疗或预防剂; 在制造治疗或预防剂中使用细胞; 一种方法，包括将细胞施用于受试者的步骤; 携带编码GITRL或GITRL衍生物的基因的病毒载体; 包含病毒载体作为活性成分的治疗或预防剂; 病毒载体在制备治疗或预防药物中的应用; 以及包括将病毒载体施用于受试者的步骤的方法。

公开(公告)号：US09603874B2

公开(公告)日：2017-03-28

申请号：US14870596

申请日：2015-09-30

申请人： MIE UNIVERSITY ,  TAKARA BIO INC.

发明人： Hiroshi Shiku ,  Hiroaki Ikeda ,  Koichi Iwamura ,  Junichi Mineno ,  Ikunoshin Kato

IPC分类号： C12N15/11 ,  A61K35/17 ,  C12N5/0783 ,  C12N15/113 ,  A61K35/12 ,  A61K39/00

CPC分类号： A61K35/17 ,  A61K2035/124 ,  A61K2039/5158 ,  C12N5/0636 ,  C12N15/113 ,  C12N2310/14 ,  C12N2501/48 ,  C12N2501/599 ,  C12N2310/531

摘要： Disclosed is a method for enhancing the function of a T cell, which is characterized by inhibiting the expression of programmed death-1 ligand 1 (PD-L1) and/or programmed death-1 ligand 2 (PD-L2) in the T cell. Also disclosed is a function-enhanced T cell which is produced by the function enhancement method. Further disclosed is a therapeutic agent comprising the function-enhanced T cell. The T cell can enhance an immune response to cancer, and is useful in an immunotherapy effective for cancer and the treatment or prevention of infectious diseases and autoimmune diseases.

公开(公告)号：US09296807B2

公开(公告)日：2016-03-29

申请号：US14701929

申请日：2015-05-01

申请人： TAKARA BIO INC. ,  MIE UNIVERSITY

发明人： Junichi Mineno ,  Sachiko Okamoto ,  Risa Sumioka ,  Masanari Kitagawa ,  Hiroshi Shiku ,  Ikunoshin Kato

IPC分类号： C12P21/06 ,  C12N5/00 ,  C12N15/63 ,  C07K14/725 ,  C12N15/85

CPC分类号： C07K14/7051 ,  C12N15/85 ,  C12N2800/40 ,  C12N2840/102

摘要： Disclosed is a cell which can express a non-natural oligomeric protein, which has, introduced therein, a gene encoding an exogenous polypeptide corresponding to at least one endogenous polypeptide constituting a natural oligomeric protein, and in which the expression of the endogenous polypeptide is inhibited.

公开(公告)号：US11714068B2

公开(公告)日：2023-08-01

申请号：US17263080

申请日：2019-07-31

申请人： Mie University

发明人： Hiroshi Shiku ,  Naohiro Seo ,  Tsuguhiro Kaneda ,  Junko Nakamura ,  Kazunari Akiyoshi

IPC分类号： G01N30/88 ,  G01N1/40

CPC分类号： G01N30/88 ,  G01N1/405 ,  G01N1/4077 ,  G01N2001/4088

摘要： The disclosure provides a method for preparing exosomes using: (i) a step for ultrafiltering a sample containing at least one exosome; and (ii) a step for subjecting the sample that can be obtained from step (i) to anion exchange column chromatography.