公开(公告)号：US09144603B2

公开(公告)日：2015-09-29

申请号：US14053997

申请日：2013-10-15

申请人： MIE UNIVERSITY ,  TAKARA BIO INC.

发明人： Hiroshi Shiku ,  Hiroaki Ikeda ,  Jun Mitsui ,  Yuki Takenaka ,  Junichi Mineno ,  Ikunoshin Kato

IPC分类号： A61K48/00 ,  A61K39/00 ,  A61K38/19 ,  C07K14/705 ,  A61K35/12 ,  A61K38/00

CPC分类号： A61K39/0005 ,  A61K35/12 ,  A61K38/00 ,  A61K38/191 ,  A61K39/0011 ,  A61K2039/5152 ,  A61K2039/5156 ,  C07K14/70575 ,  C07K2319/30 ,  C12N2799/022 ,  C12N2799/027

摘要： Disclosed are: a cell capable of expressing an exogenous GITRL or an exogenous GITRL derivative; a method for producing the cell; a therapeutic or prophylactic agent comprising the cell as an active ingredient; use of the cell in the manufacture of a therapeutic or prophylactic agent; a method comprising a step of administering the cell to a subject; a viral vector carrying a gene encoding a GITRL or a GITRL derivative; a therapeutic or prophylactic agent comprising the viral vector as an active ingredient; use of the viral vector in the manufacture of a therapeutic or prophylactic agent; and a method comprising a step of administering the viral vector to a subject.

摘要翻译： 公开了能够表达外源GITRL或外源性GITRL衍生物的细胞; 电池的制造方法; 包含该细胞作为活性成分的治疗或预防剂; 在制造治疗或预防剂中使用细胞; 一种方法，包括将细胞施用于受试者的步骤; 携带编码GITRL或GITRL衍生物的基因的病毒载体; 包含病毒载体作为活性成分的治疗或预防剂; 病毒载体在制备治疗或预防药物中的应用; 以及包括将病毒载体施用于受试者的步骤的方法。

公开(公告)号：US09603874B2

公开(公告)日：2017-03-28

申请号：US14870596

申请日：2015-09-30

申请人： MIE UNIVERSITY ,  TAKARA BIO INC.

发明人： Hiroshi Shiku ,  Hiroaki Ikeda ,  Koichi Iwamura ,  Junichi Mineno ,  Ikunoshin Kato

IPC分类号： C12N15/11 ,  A61K35/17 ,  C12N5/0783 ,  C12N15/113 ,  A61K35/12 ,  A61K39/00

CPC分类号： A61K35/17 ,  A61K2035/124 ,  A61K2039/5158 ,  C12N5/0636 ,  C12N15/113 ,  C12N2310/14 ,  C12N2501/48 ,  C12N2501/599 ,  C12N2310/531

摘要： Disclosed is a method for enhancing the function of a T cell, which is characterized by inhibiting the expression of programmed death-1 ligand 1 (PD-L1) and/or programmed death-1 ligand 2 (PD-L2) in the T cell. Also disclosed is a function-enhanced T cell which is produced by the function enhancement method. Further disclosed is a therapeutic agent comprising the function-enhanced T cell. The T cell can enhance an immune response to cancer, and is useful in an immunotherapy effective for cancer and the treatment or prevention of infectious diseases and autoimmune diseases.

公开(公告)号：US09296807B2

公开(公告)日：2016-03-29

申请号：US14701929

申请日：2015-05-01

申请人： TAKARA BIO INC. ,  MIE UNIVERSITY

发明人： Junichi Mineno ,  Sachiko Okamoto ,  Risa Sumioka ,  Masanari Kitagawa ,  Hiroshi Shiku ,  Ikunoshin Kato

IPC分类号： C12P21/06 ,  C12N5/00 ,  C12N15/63 ,  C07K14/725 ,  C12N15/85

CPC分类号： C07K14/7051 ,  C12N15/85 ,  C12N2800/40 ,  C12N2840/102

摘要： Disclosed is a cell which can express a non-natural oligomeric protein, which has, introduced therein, a gene encoding an exogenous polypeptide corresponding to at least one endogenous polypeptide constituting a natural oligomeric protein, and in which the expression of the endogenous polypeptide is inhibited.

公开(公告)号：US09175308B2

公开(公告)日：2015-11-03

申请号：US14349763

申请日：2012-10-05

申请人： MIE UNIVERSITY ,  TAKARA BIO INC.

发明人： Hiroshi Shiku ,  Yuki Orito ,  Junichi Mineno ,  Sachiko Okamoto ,  Yasunori Amaishi

IPC分类号： C12N5/10 ,  C12N5/0783 ,  C07H21/04 ,  C07K14/705 ,  C07K19/00 ,  C12N15/85 ,  C07K14/725 ,  C07K16/30

CPC分类号： C12N15/85 ,  C07K14/7051 ,  C07K14/70517 ,  C07K14/70521 ,  C07K14/70578 ,  C07K14/70596 ,  C07K16/30 ,  C07K2319/00 ,  C07K2319/03 ,  C07K2319/30

摘要： Provided are a chimeric antigen receptor comprising an extracellular domain capable of binding to an antigen, a transmembrane domain and at least one intracellular domain, the chimeric antigen receptor being characterized in that an intracellular domain of a glucocorticoid-induced tumor necrosis factor receptor (GITR) is contained as the intracellular domain; a nucleic acid encoding the chimeric antigen receptor; a cell expressing the chimeric antigen receptor; and a method for producing the cell.

摘要翻译： 提供了包含能够结合抗原，跨膜结构域和至少一个细胞内结构域的细胞外结构域的嵌合抗原受体，所述嵌合抗原受体的特征在于糖皮质激素诱导的肿瘤坏死因子受体（GITR）的细胞内结构域， 作为细胞内结构域; 编码嵌合抗原受体的核酸; 表达嵌合抗原受体的细胞; 以及该细胞的制造方法。

公开(公告)号：US08951510B2

公开(公告)日：2015-02-10

申请号：US13734625

申请日：2013-01-04

申请人： Mie University ,  Takara Bio Inc.

发明人： Hiroshi Shiku ,  Atsunori Hiasa ,  Satoshi Okumura ,  Hiroaki Naota ,  Yoshihiro Miyahara

IPC分类号： A01N63/00 ,  A01N65/00 ,  C07H21/02 ,  C12N15/00 ,  C12N5/00 ,  C12N5/02 ,  C12N5/071 ,  C07K14/705 ,  C07K14/725 ,  C12N5/0783 ,  A61K35/12 ,  A61K38/00

CPC分类号： C07K14/705 ,  A61K35/12 ,  A61K38/00 ,  C07K14/7051 ,  C12N5/0636 ,  C12N2501/515 ,  C12N2510/00

摘要： A polypeptide comprising a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 5 of Sequence Listing or a polypeptide consisting of an amino acid sequence having deletion, addition, insertion or substitution of one to several amino acid residues in the sequence, the polypeptide being capable of constituting an HLA-A24-restricted, MAGE-A4143-151-specific T cell receptor together with a polypeptide consisting of an amino acid sequence shown in SEQ ID NO: 2 of Sequence Listing.

摘要翻译： 一种多肽，其包含由序列表的SEQ ID NO：5所示的氨基酸序列组成的多肽或由序列中一至数个氨基酸残基的缺失，添加，插入或取代的氨基酸序列组成的多肽， 能够构成HLA-A24限制性MAGE-A4143-151特异性T细胞受体的多肽以及由序列表的SEQ ID NO：2所示的氨基酸序列组成的多肽。

公开(公告)号：US20140242701A1

公开(公告)日：2014-08-28

申请号：US14349763

申请日：2012-10-05

申请人： MIE UNIVERSITY ,  TAKARA BIO INC.

发明人： Hiroshi Shiku ,  Yuki Orito ,  Junichi Mineno ,  Sachiko Okamoto ,  Yasunori Amaishi

IPC分类号： C07K14/705 ,  C12N15/85 ,  C07K16/30

CPC分类号： C12N15/85 ,  C07K14/7051 ,  C07K14/70517 ,  C07K14/70521 ,  C07K14/70578 ,  C07K14/70596 ,  C07K16/30 ,  C07K2319/00 ,  C07K2319/03 ,  C07K2319/30

摘要： Provided are a chimeric antigen receptor comprising an extracellular domain capable of binding to an antigen, a transmembrane domain and at least one intracellular domain, the chimeric antigen receptor being characterized in that an intracellular domain of a glucocorticoid-induced tumor necrosis factor receptor (GITR) is contained as the intracellular domain; a nucleic acid encoding the chimeric antigen receptor; a cell expressing the chimeric antigen receptor; and a method for producing the cell.

摘要翻译： 提供了包含能够结合抗原，跨膜结构域和至少一个细胞内结构域的细胞外结构域的嵌合抗原受体，所述嵌合抗原受体的特征在于糖皮质激素诱导的肿瘤坏死因子受体（GITR）的细胞内结构域， 作为细胞内结构域; 编码嵌合抗原受体的核酸; 表达嵌合抗原受体的细胞; 以及该细胞的制造方法。

公开(公告)号：US11497768B2

公开(公告)日：2022-11-15

申请号：US16619212

申请日：2018-06-05

申请人： MIE UNIVERSITY

发明人： Hiroshi Shiku ,  Yasushi Akahori ,  Yuya Kato ,  Yoshihiro Miyahara

IPC分类号： A61K35/17 ,  C07K14/725 ,  C07K14/705 ,  C07K16/28 ,  C12N15/63 ,  A61K39/00

摘要： CAR-T cells for cancer therapy are provided with an antibody that recognizes the MAGE-A4-derived-peptide/HLA-A2 complex. The antibody includes the VH amino acid sequence of SEQ ID NO: 36 and the VL amino acid sequence of SEQ ID NO: 38. The antibody preferably is provided with the amino acid sequence of SEQ ID NO: 32. Such CAR-T cells can be used in CAR infusion therapy in which a cancer-specific intracellular antigen is used.

公开(公告)号：US11179450B2

公开(公告)日：2021-11-23

申请号：US16035922

申请日：2018-07-16

申请人： MIE UNIVERSITY ,  KYOTO UNIVERSITY

发明人： Hiroshi Shiku ,  Naozumi Harada ,  Daisuke Muraoka ,  Kazunari Akiyoshi

IPC分类号： A61K39/00 ,  A61K39/015 ,  A61K39/12 ,  A61K47/36 ,  A61K9/00

摘要： A long-chain peptide antigen includes a plurality of epitopes. An interepitope sequence located between two of the plurality of epitopes contains four to ten consecutive tyrosines. The long-chain peptide antigen may be administered to a patient together with a hydrophobized polysaccharide, such as cholesterol-modified pullulan, and/or an adjuvant, such as CpG oligo DNA.

公开(公告)号：US20210239664A1

公开(公告)日：2021-08-05

申请号：US17263080

申请日：2019-07-31

申请人： Mie University

发明人： Hiroshi Shiku ,  Naohiro Seo ,  Tsuguhiro Kaneda ,  Junko Nakamura ,  Kazunari Akiyoshi

IPC分类号： G01N30/88 ,  G01N1/40

摘要： The present invention aims to provide a method for preparing exosomes comprising: (i) a step for ultrafiltering a sample containing at least one exosome; and (ii) a step for subjecting the sample that can be obtained from step (i) to anion exchange column chromatography.

公开(公告)号：US11039908B2

公开(公告)日：2021-06-22

申请号：US15737268

申请日：2016-06-15

申请人： MIE UNIVERSITY ,  DAICEL CORPORATION

发明人： Hiroshi Shiku ,  Yuzo Yamamoto ,  Takaya Masumoto

IPC分类号： A61D1/02 ,  A61D7/00 ,  A61K39/00 ,  A61K31/7088 ,  A61M5/20 ,  A61M5/30 ,  A61K9/00

摘要： A needleless injector which can selectively induce the immune response and a method for introducing DNA including a region coding for an antigen to selectively produce an antibody in a living body of a mammal by using the needleless injector are provided. Disclosed is a needleless injector for injecting a DNA solution into an injection target area without using any injection needles. The needleless injector includes an accommodating unit which accommodates the DNA solution, a predetermined ignition device; and a predetermined nozzle unit. A temperature of the predetermined combustion product, which is provided during the pressurization, changes to a neighborhood of ordinary temperature within 20 msec after a pressure, which is applied to the DNA solution on account of the combustion of the igniter powder, reaches an initial peak discharge force during a pressurization process for discharging the DNA solution.