公开(公告)号：US09365866B2

公开(公告)日：2016-06-14

申请号：US13292953

申请日：2011-11-09

申请人： Mahito Nakanishi ,  Ken Nishimura ,  Masayuki Sano ,  Manami Ohtaka

发明人： Mahito Nakanishi ,  Ken Nishimura ,  Masayuki Sano ,  Manami Ohtaka

IPC分类号： C12N15/63 ,  C12N7/01 ,  C12N15/864 ,  C12N5/0789 ,  C12N15/86 ,  C12N5/074 ,  C12N15/113 ,  C12N15/877

CPC分类号： C12N15/86 ,  C12N5/0696 ,  C12N15/1131 ,  C12N15/8775 ,  C12N15/8776 ,  C12N2310/14 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2510/00 ,  C12N2760/18843

摘要： A reprogramming gene-loaded Sendai viral vector comprising Sendai virus genes and reprogramming genes, wherein the Sendai virus genes include an NP gene, P/C gene, M gene, F gene, HN gene and L gene, wherein each of the M gene, the F gene and the FIN gene is from a Sendai virus strain Cl.151-derived gene and wherein at least one of the M gene, the F gene and the HN gene is functionally deleted and the L gene encodes the amino-acid sequence of the L protein in which the amino-acid residue at position 1618 is valine and a method of producing the same.

摘要翻译： 包含仙台病毒基因和重编程基因的重编程基因载体仙台病毒载体，其中仙台病毒基因包括NP基因，P / C基因，M基因，F基因，HN基因和L基因，其中M基因， F基因和FIN基因来自仙台病毒株Cl.151衍生的基因，其中M基因，F基因和HN基因中的至少一个在功能上被缺失，并且L基因编码氨基酸序列 1618位氨基酸残基为缬氨酸的L蛋白及其制备方法。

公开(公告)号：US08496941B2

公开(公告)日：2013-07-30

申请号：US12792580

申请日：2010-06-02

申请人： Mahito Nakanishi ,  Ken Nishimura ,  Manami Ohtaka ,  Masayuki Sano

发明人： Mahito Nakanishi ,  Ken Nishimura ,  Manami Ohtaka ,  Masayuki Sano

IPC分类号： A61K39/12 ,  A61K39/155 ,  C12N5/00 ,  C12N15/00 ,  C12N15/86

CPC分类号： C12N5/0696 ,  C12N15/1131 ,  C12N15/86 ,  C12N15/8775 ,  C12N15/8776 ,  C12N2310/14 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2510/00 ,  C12N2760/18843

摘要： Stem cell reprogramming genes cloned into a single sustained expression-type Sendai viral vector are shown to reprogram differentiated somatic cells into induced pluripotent stem (iPS) cells without integration of vector sequences into the host cell's genome. The genes are transduced into normal differentiated somatic cells via infection with recombinant Sendai virus. After expression of the reprogramming genes and subsequent induction of pluripotency, the vector genome RNA including the reprogramming genes is removed from the cell to establish an iPS cell that is genetically identical to the parent somatic differentiated cell thus reducing the risk of tumorigenic transformation caused by random integration of vector sequences into the host genome. The method promises to provide safe, autologous iPS cells that can be used for human cell replacement and regeneration therapeutic applications.

摘要翻译： 克隆到单一持续表达型仙台病毒载体中的干细胞重编程基因显示将分化的体细胞重编程为诱导多能干细胞（iPS）细胞，而不将载体序列整合到宿主细胞的基因组中。 通过感染重组仙台病毒将基因转导入正常分化的体细胞。 在重编程基因表达和随后的多能性诱导之后，从细胞中除去包括重编程基因的载体基因组RNA以建立与亲本体细胞分化细胞遗传相同的iPS细胞，从而降低由随机引起的致瘤转化的风险 将载体序列整合到宿主基因组中。 该方法有望提供可用于人类细胞替代和再生治疗应用的安全的自体iPS细胞。

公开(公告)号：US09145564B2

公开(公告)日：2015-09-29

申请号：US12595497

申请日：2008-04-11

申请人： Ken Nishimura ,  Hiroaki Segawa ,  Mahito Nakanishi

发明人： Ken Nishimura ,  Hiroaki Segawa ,  Mahito Nakanishi

IPC分类号： C12N15/00 ,  C07H21/02 ,  C12N7/01 ,  A61K39/12 ,  A61K39/155 ,  A01N63/00 ,  C12N15/86 ,  A61K38/16 ,  C12N7/00 ,  A61K35/12 ,  A61K35/13

CPC分类号： C12N15/86 ,  A61K35/12 ,  A61K35/13 ,  A61K38/162 ,  C12N7/00 ,  C12N2760/18821 ,  C12N2760/18843

摘要： A persistently infective virus vector is produced by using a gene so modified as to encode an amino acid sequence including a valine substituted for an amino acid residue at position-1618 in the amino acid sequence for an L protein of a persistently non-infective Sendai virus. A non-transmissible, persistently infective virus vector is also produced by defecting or deleting at least one of M gene, F gene, and HN gene. These virus vectors have no cytotoxicity, can achieve the sustained gene expression over a long period of time, is safe, and is therefore useful.

摘要翻译： 通过使用如此修饰的基因来编码持续感染性病毒载体，以编码氨基酸序列，所述氨基酸序列包括取代氨基酸序列中位置1618处的氨基酸残基的氨基酸序列，用于持续非感染性仙台病毒的L蛋白 。 通过缺失或缺失M基因，F基因和HN基因中的至少一个也产生不可传播的持续感染性病毒载体。 这些病毒载体没有细胞毒性，可以长时间达到持续的基因表达，是安全的，因此是有用的。

公开(公告)号：US20100196993A1

公开(公告)日：2010-08-05

申请号：US12595497

申请日：2008-04-11

申请人： Ken Nishimura ,  Hiroaki Segawa ,  Mahito Nakanishi

发明人： Ken Nishimura ,  Hiroaki Segawa ,  Mahito Nakanishi

IPC分类号： C12N7/04 ,  C07H21/04 ,  C12N15/63

CPC分类号： C12N15/86 ,  A61K35/12 ,  A61K35/13 ,  A61K38/162 ,  C12N7/00 ,  C12N2760/18821 ,  C12N2760/18843

摘要： A persistently infective virus vector is produced by using a gene so modified as to encode an amino acid sequence including a valine substituted for an amino acid residue at position-1618 in the amino acid sequence for an L protein of a persistently non-infective Sendai virus. A non-transmissible, persistently infective virus vector is also produced by defecting or deleting at least one of M gene, F gene, and HN gene. These virus vectors have no cytotoxicity, can achieve the sustained gene expression over a long period of time, is safe, and is therefore useful.

摘要翻译： 通过使用如此修饰的基因来编码持续感染性病毒载体，以编码氨基酸序列，所述氨基酸序列包括取代氨基酸序列中位置1618处的氨基酸残基的氨基酸序列，用于持续非感染性仙台病毒的L蛋白 。 通过缺失或缺失M基因，F基因和HN基因中的至少一个也产生不可传播的持续感染性病毒载体。 这些病毒载体没有细胞毒性，可以长时间达到持续的基因表达，是安全的，因此是有用的。

公开(公告)号：US20070113681A1

公开(公告)日：2007-05-24

申请号：US11286193

申请日：2005-11-22

申请人： Ken Nishimura ,  Ian Hardcastle

发明人： Ken Nishimura ,  Ian Hardcastle

IPC分类号： G01D7/00

CPC分类号： G06F3/0414

摘要： A spatial pressure distribution sensor comprises a sensor array and a processor. The sensor array comprises an array of pressure sensors. Each of the pressure sensors is operable to generate a respective pressure signal in response to pressure applied to it. The pressure signal quantifies the pressure with greater than single-bit resolution. The processor is operable in response to the pressure signals to generate an information signal representing the spatial distribution of pressure applied to the sensor array.

摘要翻译： 空间压力分布传感器包括传感器阵列和处理器。 传感器阵列包括压力传感器阵列。 每个压力传感器可操作以响应于施加到其上的压力产生相应的压力信号。 压力信号以大于单位分辨率量化压力。 处理器响应于压力信号而可操作以产生表示施加到传感器阵列的压力的空间分布的信息信号。

公开(公告)号：US20070058707A1

公开(公告)日：2007-03-15

申请号：US11226974

申请日：2005-09-15

申请人： William Trutna ,  Steven Rosenau ,  Ken Nishimura ,  Graham Flower ,  Richard Ruby ,  Michael Frank ,  Mark Unkrich

发明人： William Trutna ,  Steven Rosenau ,  Ken Nishimura ,  Graham Flower ,  Richard Ruby ,  Michael Frank ,  Mark Unkrich

IPC分类号： H04B17/00

CPC分类号： H04W72/02 ,  H04W72/085

摘要： Systems and methods of detecting wireless channel status from acoustic discrimination of spectral content are described. In one aspect, a wireless system includes a spectrum analyzer, a detector, and a controller. The spectrum analyzer is operable to acoustically discriminate spectral content of an input electrical signal in multiple discrete frequency channels. The detector is operable to determine respective statuses of the frequency channels from the acoustically discriminated spectral content. The controller is operable to select one of the frequency channels based on the determined statuses of the frequency channels.

摘要翻译： 描述了从频谱内容的声学鉴别中检测无线信道状态的系统和方法。 一方面，无线系统包括频谱分析仪，检测器和控制器。 频谱分析仪可用于在多个离散频道中声学地区分输入电信号的频谱含量。 检测器可操作以从声学鉴别的频谱内容确定频道的各自状态。 控制器可操作以基于所确定的频率信道的状态来选择一个频率信道。

公开(公告)号：US07106512B2

公开(公告)日：2006-09-12

申请号：US11255502

申请日：2005-10-21

申请人： Rene Helbing ,  Charles D. Hoke ,  Ken Nishimura

发明人： Rene Helbing ,  Charles D. Hoke ,  Ken Nishimura

IPC分类号： G02B5/18 ,  G02B27/44

CPC分类号： G02F1/31 ,  H04Q11/0005 ,  H04Q2011/0026 ,  H04Q2011/0035

摘要： A wavelength selective switching device and method for selectively transmitting optical signals based on wavelength utilizes diffraction to spatially separate the optical signals of different wavelengths such that the optical signal of a selected wavelength can be selectively transmitted. The wavelength selective switching device selectively rotates the polarization components of the optical signals such that the polarization states of the polarization components are the same in both incoming and outgoing directions at the diffraction grating. Thus, a diffraction grating with a high grating line frequency (e.g. greater than 900 grating lines per mm for signals in the 1550 nm wavelength range) can be used for diffracting the polarization components of the optical signals in both the incoming and outgoing directions.

摘要翻译： 用于基于波长选择性地发射光信号的波长选择性开关装置和方法利用衍射来空间分离不同波长的光信号，使得可以选择性地发射所选波长的光信号。 波长选择开关器件选择性地旋转光信号的偏振分量，使得偏振分量的偏振状态在衍射光栅的入射方向和出射方向都相同。 因此，可以使用具有高光栅线频率（例如，对于1550nm波长范围内的信号来说，每毫米大于900个光栅线数）的衍射光栅来衍射光信号在入射和出射方向上的偏振分量。

公开(公告)号：US20060167731A1

公开(公告)日：2006-07-27

申请号：US11044342

申请日：2005-01-27

申请人： Ken Nishimura ,  Ian Hardcastle

发明人： Ken Nishimura ,  Ian Hardcastle

IPC分类号： G05B19/418

CPC分类号： G06Q10/109 ,  G06Q10/06314 ,  G06Q10/1095

摘要： Methods, machines, and machine-readable media storing machine-readable instructions for automatically scheduling meetings are described. In one aspect, a meeting request is received. The meeting request specifies a meeting duration, a set of non-fungible resources, and a number of required fungible resources of a specified type. A potential-meeting-time set of timeslots is determined. The potential-meeting-time set corresponding to a union of a first set of timeslots during each of which at least some of the non-fungible resources are available and a second set of timeslots corresponding to an intersection of each timeslot during which at least the specified number of fungible resources of the specified type are available. At least one timeslot having a duration at least as long as the specified meeting duration is identified in the potential-meeting-time set.

摘要翻译： 描述了存储用于自动安排会议的机器可读指令的方法，机器和机器可读介质。 一方面，接收会议请求。 会议请求规定会议期限，一组不可替代资源和指定类型的所需可替代资源的数量。 确定一个潜在会议时间的一组时隙。 所述潜在会议时间集合对应于其中至少一些非可替代资源可用的第一组时隙的并集，以及对应于每个时隙的交集的第二组时隙，其中至少 指定数量的可指定类型的可替代资源可用。 在潜在会议时间集合中识别至少一个具有至少等于指定的会议持续时间的持续时间的时隙。

公开(公告)号：US20060077552A1

公开(公告)日：2006-04-13

申请号：US11255502

申请日：2005-10-20

申请人： Rene Helbing ,  Charles Hoke ,  Ken Nishimura

发明人： Rene Helbing ,  Charles Hoke ,  Ken Nishimura

IPC分类号： G02B5/18 ,  G02B27/44

CPC分类号： G02F1/31 ,  H04Q11/0005 ,  H04Q2011/0026 ,  H04Q2011/0035

摘要： A wavelength selective switching device and method for selectively transmitting optical signals based on wavelength utilizes diffraction to spatially separate the optical signals of different wavelengths such that the optical signal of a selected wavelength can be selectively transmitted. The wavelength selective switching device selectively rotates the polarization components of the optical signals such that the polarization states of the polarization components are the same in both incoming and outgoing directions at the diffraction grating. Thus, a diffraction grating with a high grating line frequency (e.g. greater than 900 grating lines per mm for signals in the 1550 nm wavelength range) can be used for diffracting the polarization components of the optical signals in both the incoming and outgoing directions.

公开(公告)号：US20060054776A1

公开(公告)日：2006-03-16

申请号：US10938998

申请日：2004-09-10

申请人： Ken Nishimura

发明人： Ken Nishimura

IPC分类号： H01J40/14

CPC分类号： H05B33/0869 ,  H05B37/0272

摘要： In one embodiment, ones of a plurality of drive currents are modulated in accordance with ones of a plurality of unique modulation sequences. The modulated drive currents are then applied to a plurality of light emitters. Thereafter, a stream of optical measurements is obtained from a photosensor that is positioned to sense the aggregate light emitted by the light emitters. The stream of optical measurements is then correlated with the modulation sequences to extract optical responses to each of the plurality of drive currents. Finally, each drive current is regulated based on its relationship to its corresponding optical response. Related apparatus, and other methods for regulating the drive currents of a plurality of light emitters, is also disclosed.

摘要翻译： 在一个实施例中，根据多个唯一调制序列中的一个调制多个驱动电流中的一个。 然后将调制的驱动电流施加到多个发光体。 此后，从位于感测由发光体发射的聚集光的光传感器获得光学测量流。 然后将光学测量流与调制序列相关联，以提取对多个驱动电流中的每一个的光学响应。 最后，根据其对应的光学响应的​​关系来调节每个驱动电流。 还公开了用于调节多个发光体的驱动电流的相关装置和其它方法。