-
1.发明申请Method for selective removal of a substance from samples containing compounds having nucleic acid structure 审中-公开从含有具有核酸结构的化合物的样品中选择性去除物质的方法公开(公告)号：US20050267295A1
公开(公告)日：2005-12-01
申请号：US11076477
申请日：2005-03-09
IPC分类号： C12N15/09 , B01D15/00 , B01D15/08 , B01D15/36 , B01J20/32 , B01J41/20 , C12N15/10 , C07H21/04
CPC分类号： B01J20/3293 , B01D15/363 , B01J20/321 , B01J20/3219 , B01J20/3248 , B01J20/3251 , B01J20/3253 , B01J41/20 , C12N15/1003
摘要： A method for purifying a desired substance by separating from each other a substance (I) from a substance (II), one of which is the desired substance, both of which have affinity for the same ligand structure, and wherein substance (I) is smaller than substance (II). The method comprising the steps of: (i) providing substances I and II in a liquid; (ii) contacting the liquid with an adsorbent which selectively adsorbs substance I; (iii) recovering the desired substance; The adsorbent has (a) an interior part which carries a ligand structure that is capable of binding to substances I and II, and is accessible to substance I, and (b) an outer surface layer that does not adsorb substance II, and is more easily penetrated by substance I than by substance II.
摘要翻译： 通过从物质（II）彼此分离出物质（I）的方法，其中一种物质（II）是所需物质，两者对相同的配体结构具有亲和性，并且其中物质（I）为 小于物质（II）。 该方法包括以下步骤：（i）在液体中提供物质I和II; （ii）使液体与选择性吸附物质I的吸附剂接触; （iii）回收所需物质; 吸附剂具有（a）内部部分，其携带能够结合物质I和II的物质I可接近的配体结构，和（b）不吸附物质II的外表面层，并且更多 物质I易于被物质II渗透。
-
2.发明授权Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix 有权肽和核酸的色谱分离方法，以及新的高亲和力离子交换矩阵
公开(公告)号：US6090288A
公开(公告)日：2000-07-18
申请号：US117678
申请日：1998-12-29
CPC分类号： G01N30/482 , B01D15/363 , B01J41/20 , C07K1/18 , B01J2220/54
摘要： Process for separating off a peptide or a nucleic acid by an anion exchanger (I) characterized in that a) the anion exchanger (I) exhibits ligands, which (i) contain a primary, secondary or tertiary amino group and (ii) are covalently bound to an organic polymer (matrix), b) there on a carbon atom at a distance of 2 or 3 atoms away from an amino nitrogen in the ligands is a hydroxyl group or a primary, secondary or tertiary amino group, and c) the maximum elution ionic strength in the pH range 2-14 for at least one of the proteins transferrin, ovalbumin 1, ovalbumin 2, .beta.-lactoglobulin 1 and .beta.-lactoglobulin 2 on the anion exchanger is higher than the elution ionic strength required for a quaternary comparative ion exchanger.
摘要翻译： PCT No.PCT / SE97 / 00237 Sec。 371 1998年12月29日第 102（e）日期1998年12月29日PCT 1997年2月14日提交PCT公布。 第WO97 / 29825号公报 日期1997年8月21日阴离子交换剂（I）分离肽或核酸的方法，其特征在于a）阴离子交换剂（I）表现出配体，其中（i）含有伯，仲或叔氨基， （ii）与有机聚合物（基体）共价结合，b）在配位体中与氨基氮离开2或3个原子的碳原子处是羟基或伯，仲或叔氨基 ，和c）阴离子交换剂上至少一种蛋白质转运蛋白，卵白蛋白1，卵白蛋白2，β-乳球蛋白1和β-乳球蛋白2在pH范围2-14中的最大洗脱离子强度高于洗脱离子 四级比较离子交换器所需的强度。
-
公开(公告)号：US06426315B1
公开(公告)日：2002-07-30
申请号：US09380105
申请日：2000-03-14
申请人： Jan Bergstrom , Rolf Berglund , Lennart Soderberg
发明人： Jan Bergstrom , Rolf Berglund , Lennart Soderberg
IPC分类号： B01J3100
CPC分类号： C08B37/0039 , B01J20/3212 , B01J20/3234 , C08B37/0021
摘要： Process for, in layers of a porous matrix, exhibiting the groups A, introducing a functionality using a reagent I, introducing the functionality by a reaction with the groups A. The characterizing feature is that the matrix is contacted with a functional deficiency of reagent I and that conditions and reagent I are chosen so that the reaction between reagent I and the groups A is more rapid than diffusion of reagent I in the matrix.
摘要翻译： 在多孔基质的层中，显示基团A的方法，使用试剂I引入官能团，通过与基团A的反应引入官能团。表征特征是基质与试剂I的功能缺陷接触 并且选择条件和试剂I，使得试剂I和A组之间的反应比试剂I在基质中的扩散更快。
-
公开(公告)号：US5766460A
公开(公告)日：1998-06-16
申请号：US779059
申请日：1997-01-06
申请人： Jan Bergstrom , Lennart Soderberg
发明人： Jan Bergstrom , Lennart Soderberg
CPC分类号： B01D15/08 , G01N30/6004 , G01N2030/8881 , G01N30/6034 , G01N30/6047
摘要： A liquid chromatographic system comprises at least two modules, at least one fluid flow line, and at least two connector devices provided along the flow line and respectively connecting the modules to the flow line. One of the two modules comprises a separation module and the other of the two modules comprises an ancillary module having a non-separation function. Each of the two modules includes at least one end connector fit into the respective connector device, and each end connector is adapted to interchangeably fit into either connector device.
摘要翻译： 液相色谱系统包括至少两个模块，至少一个流体流动线，以及沿着流动管线设置并分别将模块连接到流动管线的至少两个连接器装置。 两个模块之一包括分离模块，并且两个模块中的另一个模块包括具有非分离功能的辅助模块。 两个模块中的每一个包括配合到相应连接器装置中的至少一个端部连接器，并且每个端部连接器适于可互换地装配到任一连接器装置中。
-
公开(公告)号：US08574437B2
公开(公告)日：2013-11-05
申请号：US13575750
申请日：2011-02-16
IPC分类号： B01D15/08
CPC分类号： B01J20/3293 , B01D15/361 , B01D15/3804 , B01J20/28004 , B01J20/286 , B01J20/3206 , B01J20/3248 , B01J20/3285 , B01J39/26 , B01J41/20 , C07K1/18
摘要： The present invention relates to a method of producing novel chromatography media and use thereof for purification of biomolecules, such as proteins. The chromatography media comprises shell beads having an inner porous core and an outer shell. The method comprises providing buffering ligands in the core of the beads, and providing binding ligands aimed for biomolecule binding in the outer shell of the beads. This method makes it possible to optimize binding properties and buffering properties independently of each other which is especially to advantageous for production of chromatofocusing media.
摘要翻译： 本发明涉及生产新型色谱介质的方法及其用于生物分子如蛋白质的纯化的用途。 色谱介质包括具有内多孔芯和外壳的壳珠。 该方法包括在珠的核心中提供缓冲配体，并提供靶向用于珠粒外壳中的生物分子结合的结合配体。 该方法使得可以彼此独立地优化结合性质和缓冲性质，这特别有利于生产色谱聚焦介质。
-
6.发明授权Method in a pulsed accelerator for accelerating a magnetized rotating plasma 失效用于加速磁化旋转等离子体的脉冲加速器中的方法
公开(公告)号：US5300861A
公开(公告)日：1994-04-05
申请号：US32721
申请日：1993-03-16
CPC分类号： H05H1/52
摘要： The invention provides in an accelerator for accelerating a magnetized rotating plasma comprising a magnetic system arrange symmetrically around an axis, two electrodes (10, 11) extending symmetrically along said axis inside the magnetic system, said electrodes being spaced from each other in the transverse direction of said axis, two pulsed power sources connected to the magnetic system and the electrodes, respectively, and openings (18) in the inner electrode in a cross-section perpendicular to said axis for the supply of a neutral gas to the space defined by said electrodes, a method for controlling the operation of the accelerator wherein the magnetic field is confined to form a layer which comprises a first cylindrical portion (12) with a minor diameter and a second cylindrical portion (14) with a major diameter and a transition portion (13) interconnecting said first and second cylindrical portions being arranged axis-symmetrically around a common axis.
摘要翻译： 本发明提供一种用于加速磁化旋转等离子体的加速器，该磁化旋转等离子体包括围绕轴线对称布置的磁体系统，在磁性系统内部沿所述轴对称延伸的两个电极（10,11），所述电极在横向彼此间隔开 的两个脉冲电源分别连接到磁系统和电极，以及垂直于所述轴线的横截面中的内电极中的开口（18），用于向由所述轴线限定的空间供应中性气体 电极，用于控制加速器的操作的方法，其中磁场被限制以形成包括具有小直径的第一圆柱形部分（12）和具有大直径和过渡部分的第二圆柱形部分（14）的层 将所述第一和第二圆柱形部分互相连接的连接件（13）围绕公共轴线对称布置。
-
公开(公告)号：US08070958B2
公开(公告)日：2011-12-06
申请号：US12692671
申请日：2010-01-25
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
摘要翻译： 本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。 在最有利的实施方案中，化学梯度是配体密度梯度。 本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。
-
公开(公告)号：US20090281288A1
公开(公告)日：2009-11-12
申请号：US12506445
申请日：2009-07-21
申请人： JAMES VAN ALSTINE , Jan Bergstrom , Henrik Ihre , Tobias Soderman
发明人： JAMES VAN ALSTINE , Jan Bergstrom , Henrik Ihre , Tobias Soderman
CPC分类号： B01J20/3278 , B01D15/32 , B01D15/362 , B01D15/38 , B01D15/424 , B01J20/286 , B01J20/288 , B01J20/321 , B01J20/3212 , B01J20/327 , B01J20/328
摘要： The present invention relates to a separation matrix comprised of a support to the surfaces of which polymer chains have been coupled, wherein each polymer chain presents recurring proton-donating groups and at least the surface of the support is substantially hydrophilic. In the most advantageous embodiment, the support is porous cross-linked agarose, the polymers are poly(acrylic acid) and the proton-donating groups are carboxyl groups. The matrix is useful e.g. to remove PEG from pegylated and/or native compounds in a liquid. Accordingly, the invention also encompasses a method, such as a chromatographic method, wherein the separation matrix according to the invention is used, for example as a pre-treatment of a reaction mixture that comprises unreacted PEG, pegylated proteins and native proteins.
摘要翻译： 本发明涉及一种分离基质，其由其上已经连接有聚合物链的表面的载体组成，其中每个聚合物链呈现重复的质子供体基团，并且载体的至少表面基本上是亲水的。 在最有利的实施方案中，载体是多孔交联琼脂糖，聚合物是聚（丙烯酸），质子供体基团是羧基。 该矩阵是有用的。 以从液体中的聚乙二醇化和/或天然化合物中除去PEG。 因此，本发明还包括诸如色谱方法的方法，其中使用根据本发明的分离基质，例如作为包含未反应的PEG，聚乙二醇化蛋白质和天然蛋白质的反应混合物的预处理。
-
公开(公告)号：US20070161000A1
公开(公告)日：2007-07-12
申请号：US10589740
申请日：2005-02-21
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , B01D15/327 , B01D15/34 , B01D15/363 , B01J41/20
摘要： The present invention relates to a method of isolating at least one plasmid from other components of a liquid, which method comprises the steps of providing a separation matrix comprised of one or more porous carriers, which carrier(s) present anion exchange groups on external surfaces as well as pore surfaces and a pore size distribution that does not allow access of plasmids to pore surfaces; contacting said matrix with the liquid to allow adsorption of the plasmids to ligands present on the separation matrix; contacting an eluent with the separation matrix to release the plasmids and recovering plasmids from a fraction of said eluent. Thus, the present method allows the plasmids to adsorb to the external surfaces of the matrix, while other components such as RNA is adsorbed onto the pore surfaces. In one embodiment, the matrix presents a DNA exclusion limit of at least about 270 base pairs; such as at least about 1,000 base pairs.
摘要翻译： 本发明涉及从液体的其它组分分离至少一种质粒的方法，该方法包括以下步骤：提供由一种或多种多孔载体组成的分离基质，该载体在外表面上存在阴离子交换基团 以及孔表面和不允许质粒进入孔表面的孔径分布; 使所述基质与液体接触以允许质粒吸附到存在于分离基质上的配体; 将洗脱液与分离基质接触以释放质粒并从一部分所述洗脱液中回收质粒。 因此，本方法允许质粒吸附到基质的外表面，而诸如RNA的其它组分被吸附到孔表面上。 在一个实施方案中，所述基质呈现至少约270个碱基对的DNA排除极限; 例如至少约1,000个碱基对。
-
公开(公告)号：US20070125711A1
公开(公告)日：2007-06-07
申请号：US10575945
申请日：2004-11-01
IPC分类号： B01D15/08
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
摘要翻译： 本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。 在最有利的实施方案中，化学梯度是配体密度梯度。 本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。
-
-
-
-
-
-
-
-
-
搜索结果
23 条记录 (耗时 0.036 秒)
