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公开(公告)号：US08070958B2
公开(公告)日：2011-12-06
申请号：US12692671
申请日：2010-01-25
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
摘要翻译： 本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。 在最有利的实施方案中，化学梯度是配体密度梯度。 本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。
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公开(公告)号：US20070125711A1
公开(公告)日：2007-06-07
申请号：US10575945
申请日：2004-11-01
IPC分类号： B01D15/08
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
摘要翻译： 本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。 在最有利的实施方案中，化学梯度是配体密度梯度。 本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。
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公开(公告)号：US20110045574A1
公开(公告)日：2011-02-24
申请号：US12988707
申请日：2009-04-21
申请人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman , Tobias E. Soderman
发明人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman , Tobias E. Soderman
IPC分类号： C12N7/02 , H01F1/00 , C12N1/20 , C12N15/63 , B01J20/00 , B01J20/26 , C07K16/00 , C07K1/14 , C07H21/00 , C11B3/00
CPC分类号： B01D15/327 , B01J20/28009 , B01J20/285 , B01J20/287 , B01J20/3212 , B01J20/3293 , B01J39/26 , C07K1/20 , C07K16/00 , C12N7/00 , C12N2760/16151 , G01N1/405
摘要： The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.
摘要翻译： 本发明在色谱领域内。 更准确地说，它涉及一种新颖的色谱介质，即由于疏水介质的孔隙率和/或盖的孔隙度，提供了不同盖子的疏水介质，其中排除了一定尺寸的分子。 本发明还涉及用于纯化不分离介质的大分子的分离介质以及进入分离介质并从其中洗脱的小分子的用途。
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公开(公告)号：US20070112178A1
公开(公告)日：2007-05-17
申请号：US10589718
申请日：2005-02-24
CPC分类号： C07K1/18 , B01D15/362 , B01D15/3809 , B01D15/3847 , B01J39/07 , B01J39/26 , B01J47/014 , C07K1/165 , B01D15/327
摘要： The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.
摘要翻译： 本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个 阳离子交换基团和至少一个芳族或杂芳族环系统。 在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。 本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。
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公开(公告)号：US20110118442A1
公开(公告)日：2011-05-19
申请号：US12984132
申请日：2011-01-04
申请人： CARINA ENGSTRAND , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J. Johansson , Jean-Luc Maloisel
发明人： CARINA ENGSTRAND , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J. Johansson , Jean-Luc Maloisel
IPC分类号： C07K1/16 , C07C211/00 , A61L2/00 , B01D15/08
CPC分类号： B01D15/3828 , B01D15/327 , B01D15/363 , B01D15/3804 , B01D15/3809 , B01D15/3847 , B01J20/286 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/165 , C07K1/36 , C07K16/00 , C07K16/065
摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
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公开(公告)号：US07714112B2
公开(公告)日：2010-05-11
申请号：US11577203
申请日：2005-10-21
申请人： Carina Engstrand , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J. Johansson , Jean-Luc Maloisel
发明人： Carina Engstrand , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J. Johansson , Jean-Luc Maloisel
CPC分类号： B01D15/3828 , B01D15/327 , B01D15/363 , B01D15/3804 , B01D15/3809 , B01D15/3847 , B01J20/286 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/165 , C07K1/36 , C07K16/00 , C07K16/065
摘要： The present invention relates to a method of separating antibodies from other compound(s) in a liquid sample, wherein a mobile phase comprising said sample is contacted with a multi-modal separation matrix to adsorb undesired compounds while the antibodies remain free in the liquid, wherein the multi-modal separation matrix comprises first groups, which are capable of interacting with negatively charged sites of the target compounds, and second groups, which are capable of at least one interaction other than charge-charge interaction with said target compounds. The invention also relates to a chromatography column packed with the above-described multi-modal separation matrix and a filter having such multi-modal groups adsorbed to its surface.
摘要翻译： 本发明涉及从液体样品中的其它化合物分离抗体的方法，其中包含所述样品的流动相与多模式分离基质接触以吸附不期望的化合物，同时抗体在液体中保持游离， 其中所述多模式分离基质包含能够与所述目标化合物的带负电荷位点相互作用的第一组和能够与所述目标化合物的电荷 - 电荷相互作用以外的至少一个相互作用的第二组。 本发明还涉及一种填充有上述多模式分离基质的色谱柱，以及具有吸附在其表面上的多模式基团的过滤器。
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公开(公告)号：US08895710B2
公开(公告)日：2014-11-25
申请号：US12984132
申请日：2011-01-04
申请人： Carina Engstrand , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J. Johansson , Jean-Luc Maloisel
发明人： Carina Engstrand , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J. Johansson , Jean-Luc Maloisel
IPC分类号： C07K1/14 , B01D15/36 , B01D15/38 , B01J20/286 , B01J41/20 , B01J20/32 , B01D15/32 , C07K16/06 , C07K1/36
CPC分类号： B01D15/3828 , B01D15/327 , B01D15/363 , B01D15/3804 , B01D15/3809 , B01D15/3847 , B01J20/286 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/165 , C07K1/36 , C07K16/00 , C07K16/065
摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
摘要翻译： 本发明涉及由下式R1-R2-N（R3）-R4-R5定义的色谱配体，其中R1是取代或未取代的苯基; R2是包含0-4个碳原子的烃链; R3是包含1-3个碳原子的烃链; R4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜耦合的所述配体。 根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。
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公开(公告)号：US20110155668A1
公开(公告)日：2011-06-30
申请号：US13002878
申请日：2009-06-04
CPC分类号： B01D15/3828 , B01D15/166 , B01D15/36 , B01J20/28092 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3293 , C07K1/16 , C07K1/22 , C07K16/00
摘要： The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.
摘要翻译： 本发明涉及一种分离介质，其包括具有带电配位体的多孔材料的内核和包含带有带电配位体的多孔材料的外盖，其中内芯中配体的电荷与 盖中配体的电荷。 本发明还涉及一种用于生物分子分离的方法，包括将样品施加到上述分离介质中，其中通过从介质的电荷排斥来防止大分子进入介质，并且小分子被捕获在内核中。
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公开(公告)号：US20070196858A1
公开(公告)日：2007-08-23
申请号：US11570530
申请日：2005-06-22
CPC分类号： B01J20/3212 , B01D15/426 , B01J20/286 , B01J20/289 , B01J20/3219 , B01J20/3251
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilised, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.
摘要翻译： 本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一个磺酰胺，并且磺酰基的R基包括芳族基团。 磺酰胺的氮可以是仲胺或叔胺。 本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到分离基质上分离免疫球蛋白样化合物的方法。
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公开(公告)号：US20140017813A1
公开(公告)日：2014-01-16
申请号：US14008001
申请日：2012-03-29
IPC分类号： G01N1/40
CPC分类号： G01N1/405 , B01D15/34 , B01D15/3866 , B01D15/3885 , B01J20/28009 , B01J20/285 , B01J20/3204 , B01J20/3227 , B01J20/3274 , B01J20/3293 , G01N30/14 , G01N2030/143
摘要： The present invention relates to a method for depletion of undesired molecules and/or enrichment of desired molecules from a sample comprising high abundant as well as low abundant molecules, comprising the following steps: a) providing a separation material comprising a solid phase (beads) comprising an inner porous core material comprising magnetic particles and an outer porous shell with a porosity equal or denser than that of the shell; b) adding the sample to the separation material; c) adsorbing a first fraction of molecules with a molecular weight of 500-50 000 Da in the core and simultaneously excluding a second fraction of molecules from binding to the core and the shell, wherein the molecular weight of the second fraction molecules is at least 5 preferably 10 times higher than the molecular weight of the first fraction and d) eluting the desired molecules from the separation material, wherein step d) and optionally step c) is performed using an oscillating power/field applied over the separation material.The first fraction of molecules are for example drugs with a mw of about 700 Da, small proteins/peptides with an mw of about 7000 Da or proteins with a mw of about 40 000 Da.
摘要翻译： 本发明涉及从包含高丰度和低丰度分子的样品中消耗不想要的分子和/或富集所需分子的方法，包括以下步骤：a）提供包含固相（珠） 包括包含磁性颗粒的内部多孔芯材料和具有等于或比密封件的孔隙度更高的孔隙的外部多孔壳体; b）将样品加入到分离材料中; c）在核心中吸附分子量为500-500Da的第一级分子，同时排除第二级分子与核心和壳结合，其中第二级分子的分子量至少为 5，优选比第一级分分子量高10倍; d）从分离材料洗脱所需的分子，其中步骤d）和任选的步骤c）使用施加在分离材料上的振荡功率/场进行。 分子的第一部分是例如mw为约700Da的药物，具有约7000Da的mw的小蛋白质/肽或约40,000Da的蛋白质。
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