RABIES VIRUS LIKE PARTICLE PRODUCTION IN PLANTS
    5.
    发明申请
    RABIES VIRUS LIKE PARTICLE PRODUCTION IN PLANTS 审中-公开
    RABIES病毒样植物生产

    公开(公告)号:US20140227322A1

    公开(公告)日:2014-08-14

    申请号:US14126357

    申请日:2012-06-13

    IPC分类号: C07K14/005

    摘要: A method of producing a virus like particle (VLP) in a plant is provided. The method comprises introducing a first nucleic acid into the plant, or portion of the plant. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding a native rabies virus structural protein. The nucleotide sequence may further comprise one or more than one amplification element. Optionally, a second nucleic acid might be introduced into the plant, or portion of the plant. The second nucleic acid comprising a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding a matrix protein, for example but not limited to a rabies matrix protein. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby producing the VLP.

    摘要翻译: 提供了一种在植物中生产病毒样颗粒(VLP)的方法。 该方法包括将第一核酸引入植物或植物的一部分。 所述第一核酸包含在植物中活性的第一调节区,其与编码天然狂犬病病毒结构蛋白的核苷酸序列有效连接。 核苷酸序列还可以包含一个或多于一个的扩增元件。 任选地,第二核酸可以被引入植物或植物的一部分。 所述第二核酸包含在植物中活性的第二调节区,并与编码基质蛋白(例如但不限于狂犬病基质蛋白)的核苷酸序列有效连接。 将植物或植物的一部分在允许核酸表达的条件下温育,从而产生VLP。

    METHOD OF PREPARING PLANT-DERIVED PROTEINS
    7.
    发明申请
    METHOD OF PREPARING PLANT-DERIVED PROTEINS 审中-公开
    制备植物衍生蛋白的方法

    公开(公告)号:US20130067807A1

    公开(公告)日:2013-03-21

    申请号:US13497767

    申请日:2010-09-21

    摘要: Methods of preparing plant-derived proteins or suprastructure proteins, are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized proteins, or suprastructure proteins, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived proteins or suprastructure proteins. Alternatively, the proteins, or suprastructure proteins, may be obtained from plant or plant matter comprising plant-derived proteins or suprastructure proteins, by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived proteins or suprastructure proteins, are recovered from the filtered fraction.

    摘要翻译: 提供了制备植物衍生蛋白或超基质蛋白的方法。 该方法可以包括获得植物或包含质外体蛋白质定位蛋白质的植物物质或超基质蛋白,从植物或植物物质产生原生质体/原生质体级分和质外体级分,并回收载脂蛋白级分。 载脂蛋白级分包含植物来源的蛋白质或超基因蛋白质。 或者,可以通过使用细胞壁降解酶组合物消化植物物质以产生消化部分,从包含植物衍生的蛋白质或超基质蛋白质的植物或植物物质获得蛋白质或超基质蛋白质。 将消化的馏分过滤以产生过滤的馏分,从过滤的馏分中回收植物来源的蛋白质或超结构蛋白质。

    METHOD OF PREPARING PLANT-DERIVED VLPS
    8.
    发明申请
    METHOD OF PREPARING PLANT-DERIVED VLPS 审中-公开
    制备植物衍生VLPS的方法

    公开(公告)号:US20120178149A1

    公开(公告)日:2012-07-12

    申请号:US13497757

    申请日:2010-09-21

    IPC分类号: C12S3/14

    摘要: Methods of preparing plant-derived virus like particles (VLPs) are provided. The method may comprise obtaining a plant, or plant matter comprising apoplast-localized VLPs, producing a protoplast/spheroplast fraction and apoplast fraction from the plant or plant matter, and recovering the apoplast fraction. The apoplast fraction comprises plant-derived VLPs. Alternatively, VLPs may be obtained from plant or plant matter comprising plant-derived VLPs by digesting the plant matter using a cell wall degrading enzyme composition to produced a digested fraction. The digested fraction is filtered to produced a filtered fraction, and the plant-derived VLPs are recovered from the filtered fraction.

    摘要翻译: 提供了植物来源的病毒样颗粒(VLPs)的制备方法。 该方法可以包括获得植物或包含原生质体定位的VLP的植物物质,从植物或植物物质产生原生质体/原生质体级分和原生质体级分,以及回收载脂蛋白级分。 载脂蛋白级分包含植物来源的VLP。 或者,VLP可以通过使用细胞壁降解酶组合物消化植物物质从包含植物来源的VLP的植物或植物物质获得,以产生消化部分。 将消化的馏分过滤以产生过滤的馏分,从过滤的馏分中回收来自植物的VLP。