公开(公告)号：US20050233440A1

公开(公告)日：2005-10-20

申请号：US11092415

申请日：2005-03-29

Applicant: Mario Scurati ,  Ubaldo Mastromatteo ,  Michele Palmieri

Inventor： Mario Scurati ,  Ubaldo Mastromatteo ,  Michele Palmieri

IPC: B01F13/00 ,  B01L3/00 ,  B01L7/00 ,  B03C5/02 ,  G01N27/447 ,  C12M1/34 ,  C12Q1/68

CPC classification number: G01N27/44704 ,  B01F13/0059 ,  B01L3/502715 ,  B01L7/525 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/049 ,  B01L2400/0677 ,  B03C5/026 ,  B03C2201/26

Abstract: An integrated device for nucleic acid analysis having a support and a first tank for introducing a raw biological specimen includes at least one pre-treatment channel, a buried amplification chamber, and a detection chamber carried by the support and in fluid connection with one another and with the tank. The device can be used for all types of biological analyses.

Abstract translation: 用于具有支撑物和用于引入原始生物样本的第一罐的用于核酸分析的集成装置包括至少一个预处理通道，掩埋放大室和由支撑件承载并彼此流体连接的检测室，以及 与坦克。 该设备可用于所有类型的生物分析。

公开(公告)号：US20070125650A1

公开(公告)日：2007-06-07

申请号：US11531679

申请日：2006-09-13

Applicant: Mario Scurati ,  Torsten Mueller ,  Thomas Schnelle

Inventor： Mario Scurati ,  Torsten Mueller ,  Thomas Schnelle

IPC: B03C5/02

CPC classification number: B03C5/026

Abstract: A plurality of planar electrodes (5) in a microchannel (4) is used for separation, lysis and PCR in a chip (10). Cells from a sample are brought to the electrodes (5). Depending on sample properties, phase pattern, frequency and voltage of the electrodes and flow velocity are chosen to trap target cells (16) using DEP, whereas the majority of unwanted cells (17) flushes through. After separation the target cell (16) are lysed while still trapped. Lysis is carried out by applying RF pulses and/or thermally so as to change the dielectric properties of the trapped cells. After lysis, the target cells (16) are amplified within the microchannel (4), so as to obtain separation, lysis and PCR on same chip (1).

Abstract translation: 微通道（4）中的多个平面电极（5）用于芯片（10）中的分离，裂解和PCR。 来自样品的细胞被带到电极（5）。 根据样品性质，选择电极的相图，频率和电压以及流速，以使用DEP捕获靶细胞（16），而大多数不需要的细胞（17）冲洗通过。 分离后，靶细胞（16）裂解，同时仍被捕获。 通过施加RF脉冲和/或热来进行裂解以改变被捕获的细胞的介电性质。 裂解后，在微通道（4）内扩增靶细胞（16），以在同一芯片（1）上获得分离，裂解和PCR。

公开(公告)号：US07794611B2

公开(公告)日：2010-09-14

申请号：US12019630

申请日：2008-01-24

Applicant: Mario Scurati

Inventor： Mario Scurati

IPC: C23F1/00

CPC classification number: F04B43/043 ,  B01L3/50273 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/049 ,  B01L2400/0677

Abstract: A micropump includes a body (10) of semiconductor material, accommodating fluid-tight chambers (32), having an internal preset pressure, lower than atmospheric pressure. The fluid-tight chambers (32), sealed by a diaphragm (35) that can be electrically opened, are selectively openable using a first electrode (37) and second electrodes (38), accommodating between them portions of the diaphragm (35).

Abstract translation: 微型泵包括半导体材料的本体（10），其容纳具有低于大气压的内部预设压力的流体密封室（32）。 由能够电打开的隔膜（35）密封的流体密封室（32）可以使用容纳在隔膜（35）的部分之间的第一电极（37）和第二电极（38）选择性地打开。

公开(公告)号：US07988841B2

公开(公告)日：2011-08-02

申请号：US11531679

申请日：2006-09-13

Applicant: Mario Scurati ,  Torsten Mueller ,  Thomas Schnelle

Inventor： Mario Scurati ,  Torsten Mueller ,  Thomas Schnelle

IPC: B03C5/02

CPC classification number: B03C5/026

Abstract: A plurality of planar electrodes (5) in a microchannel (4) is used for separation, lysis and PCR in a chip (10). Cells from a sample are brought to the electrodes (5). Depending on sample properties, phase pattern, frequency and voltage of the electrodes and flow velocity are chosen to trap target cells (16) using DEP, whereas the majority of unwanted cells (17) flushes through. After separation the target cell (16) are lysed while still trapped. Lysis is carried out by applying RF pulses and/or thermally so as to change the dielectric properties of the trapped cells. After lysis, the target cells (16) are amplified within the microchannel (4), so as to obtain separation, lysis and PCR on same chip (1).

Abstract translation: 微通道（4）中的多个平面电极（5）用于芯片（10）中的分离，裂解和PCR。 来自样品的细胞被带到电极（5）。 根据样品性质，选择电极的相图，频率和电压以及流速，以使用DEP捕获靶细胞（16），而大多数不需要的细胞（17）冲洗通过。 分离后，靶细胞（16）裂解，同时仍被捕获。 通过施加RF脉冲和/或热来进行裂解以改变被捕获的细胞的介电性质。 裂解后，在微通道（4）内扩增靶细胞（16），以在同一芯片（1）上获得分离，裂解和PCR。

公开(公告)号：US07527480B2

公开(公告)日：2009-05-05

申请号：US10663239

申请日：2003-09-16

Applicant: Mario Scurati

Inventor： Mario Scurati

IPC: F04F3/00 ,  C12M1/34 ,  B01L3/02

CPC classification number: F04B43/043 ,  B01L3/50273 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/049 ,  B01L2400/0677

Abstract: A micropump includes a body (10) of semiconductor material, accommodating fluid-tight chambers (32), having an internal preset pressure, lower than atmospheric pressure. The fluid-tight chambers (32), sealed by a diaphragm (35) that can be electrically opened, are selectively openable using a first electrode (37) and second electrodes (38), accommodating between them portions of the diaphragm (35).

Abstract translation: 微型泵包括半导体材料的本体（10），其容纳具有低于大气压的内部预设压力的流体密封室（32）。 由能够电打开的隔膜（35）密封的流体密封室（32）可以使用容纳在隔膜（35）的部分之间的第一电极（37）和第二电极（38）选择性地打开。

公开(公告)号：US20090010805A1

公开(公告)日：2009-01-08

申请号：US12119948

申请日：2008-05-13

Applicant: Jeffrey RAYNOR ,  Mario Scurati

Inventor： Jeffrey RAYNOR ,  Mario Scurati

IPC: B01J19/00

CPC classification number: F16K99/0032 ,  B01L3/50273 ,  B01L2400/049 ,  B01L2400/0677 ,  F16K99/0044 ,  F16K2099/008 ,  F16K2099/0084

Abstract: An assaying device includes one or more detectors, a transporter and inlet that is connected to the one or more detectors by a one or more channels. The transporter includes one or more sealed, vacuum-containing chambers being connected to the channels, wherein each of the chambers includes an electrically activated puncture. The puncture is configured to puncture a wall of a chamber and cause a differential pressure in the one ore more channels, and thereby transport a fluid from the inlet to the one or more detectors.

Abstract translation: 测定装置包括一个或多个检测器，通过一个或多个通道连接到所述一个或多个检测器的运送器和入口。 输送器包括连接到通道的一个或多个密封的真空容纳室，其中每个室包括电活化穿刺。 穿刺被配置为刺穿腔室的壁并在一个或多个通道中产生压差，从而将流体从入口运送到一个或多个检测器。

公开(公告)号：US20080138210A1

公开(公告)日：2008-06-12

申请号：US12019630

申请日：2008-01-24

Applicant: Mario Scurati

Inventor： Mario Scurati

IPC: F04B37/02

CPC classification number: F04B43/043 ,  B01L3/50273 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/049 ,  B01L2400/0677

Abstract: A micropump includes a body (10) of semiconductor material, accommodating fluid-tight chambers (32), having an internal preset pressure, lower than atmospheric pressure. The fluid-tight chambers (32), sealed by a diaphragm (35) that can be electrically opened, are selectively openable using a first electrode (37) and second electrodes (38), accommodating between them portions of the diaphragm (35).

Abstract translation: 微型泵包括半导体材料的本体（10），其容纳具有低于大气压的内部预设压力的流体密封室（32）。 由能够电打开的隔膜（35）密封的流体密封室（32）可以使用容纳在隔膜（35）的部分之间的第一电极（37）和第二电极（38）选择性地打开。

公开(公告)号：US06538346B2

公开(公告)日：2003-03-25

申请号：US09740726

申请日：2000-12-18

Applicant: Albino Pidutti ,  Mario Scurati

Inventor： Albino Pidutti ,  Mario Scurati

IPC: H01B124

CPC classification number: H02M3/156 ,  H02M2003/1555 ,  Y02B70/1425 ,  Y10T307/50 ,  Y10T307/76 ,  Y10T307/826 ,  Y10T307/839

Abstract: A circuit to control the supply of a reactive load, for supplying variable quantities of energy to the load in a predetermined manner is included in a system. The system also includes reactive components which are connected to the load by way of a controllable electronic switch and which form a resonant circuit with the load when the electronic switch is closed. Further, the system includes a circuit for activating the electronic switch, and a control unit which coordinates the operation of the controlled supply circuit and of the activation circuit in accordance with a predetermined program. The system enables the load to be driven with a particularly low power dissipated.

Abstract translation: 用于控制用于以预定方式向负载供应可变量的能量的无功负载的供应的电路被包括在系统中。 该系统还包括通过可控电子开关连接到负载并且当电子开关闭合时与负载形成谐振电路的无功部件。 此外，该系统包括用于激活电子开关的电路，以及控制单元，其根据预定程序协调受控供电电路和激活电路的操作。 该系统能够以特别低的功率耗散驱动负载。

公开(公告)号：US06181031B2

公开(公告)日：2001-01-30

申请号：US09200297

申请日：1998-11-25

Applicant: Albino Pidutti ,  Mario Scurati

Inventor： Albino Pidutti ,  Mario Scurati

IPC: H01B124

CPC classification number: H05B33/08 ,  Y10T307/839

Abstract: A circuit to control the supply of a reactive load, for supplying variable quantities of energy to the load in a predetermined manner is included in a system. The system also includes reactive components which are connected to the load by way of a controllable electronic switch and which form a resonant circuit with the load when the electronic switch is closed. Further, the system includes a circuit for activating the electronic switch, and a control unit which coordinates the operation of the controlled supply circuit and of the activation circuit in accordance with a predetermined program. The system enables the load to be driven with a particularly low power dissipated.

Abstract translation: 用于控制用于以预定方式向负载供应可变量的能量的无功负载的供应的电路被包括在系统中。 该系统还包括通过可控电子开关连接到负载并且当电子开关闭合时与负载形成谐振电路的无功部件。 此外，该系统包括用于激活电子开关的电路，以及控制单元，其根据预定程序协调受控供电电路和激活电路的操作。 该系统能够以特别低的功率耗散驱动负载。

公开(公告)号：US5589827A

公开(公告)日：1996-12-31

申请号：US233120

申请日：1994-04-26

Applicant: Mario Scurati

Inventor： Mario Scurati

IPC: G08G1/01 ,  B60W30/00 ,  G08G1/09 ,  G08G1/0962 ,  G08G1/16 ,  G08B1/00

CPC classification number: G08G1/163

Abstract: An interactive method for monitoring road traffic consisting of detecting, using a short-range receiver installed on a vehicle, the presence of preceding vehicles in the same running direction and their dynamic conditions, as transmitted by the preceding vehicles, in the form of binary coded periodic message at nonoverlapped time windows for each vehicle. The method further consists of transmitting, to the following vehicles using a short-range transmitter installed on the vehicle, a binary coded message indicating the presence of the vehicle and, optionally, dynamic conditions of the preceding vehicles, at time windows non-overlapping the transmission time windows of the preceding vehicles.

Abstract translation: 一种用于监测道路交通的交互方法，包括使用安装在车辆上的短距离接收机，以相同的运行方向存在先前的车辆及其动态条件，如由前面的车辆传送的，以二进制编码的形式 每个车辆的非重叠时间窗口的周期性消息。 该方法还包括：使用安装在车辆上的短距离发射机向以下车辆发送指示车辆存在的二进制编码消息，以及可选地，前一车辆的动态条件，在不重叠的时间窗口 前车的传输时间窗。