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公开(公告)号：US08536099B2
公开(公告)日：2013-09-17
申请号：US12872333
申请日：2010-08-31
申请人： Mark Oldham , George Fry , Christina Inman , John Bridgham , Timothy Hunkapillar , Charles Vann
发明人： Mark Oldham , George Fry , Christina Inman , John Bridgham , Timothy Hunkapillar , Charles Vann
CPC分类号： C12Q1/6869 , B01F13/0059 , B01F13/0222 , B01J19/0046 , B01J2219/00317 , B01J2219/00466 , B01J2219/00527 , B01J2219/00576 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00722 , B01L3/502707 , B01L3/50273 , B01L2200/027 , B01L2200/0647 , B01L2200/0684 , B01L2200/16 , B01L2300/0819 , B01L2300/0822 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/1822 , B01L2400/0427 , C12Q1/6874 , G01N35/00069 , G01N35/1002 , G01N35/1097 , G01N2035/00148 , G01N2035/00237 , G01N2035/1034 , G02B21/34 , Y10T436/11 , Y10T436/143333
摘要： According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
摘要翻译： 根据各种实施方案，提供了一种方法，其包括用洗涤溶液洗涤基底上的DNA包被的珠粒阵列以从基底去除堆叠的珠粒。 洗涤溶液可以在载体中包括惰性固体珠。 DNA包被的珠可以具有平均直径，并且洗涤溶液中的固体珠的平均直径可以是DNA包被的珠的直径的至少两倍。 洗涤可以形成脱落的DNA包被的珠粒和单层DNA包被的珠粒。 在一些实施方案中，用于形成阵列的第一珠粒与包含分子量约350Da或更低的PEG的聚（乙二醇）（PEG）溶液接触。 在一些实施例中，用于形成珠阵列的滑块被提供为用于对其进行成像的系统。
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公开(公告)号：US20110136677A1
公开(公告)日：2011-06-09
申请号：US12872333
申请日：2010-08-31
申请人： Mark OLDHAM , George Fry , Christina Inman , John Bridgham , Timothy Hunkapillar , Charles Vann
发明人： Mark OLDHAM , George Fry , Christina Inman , John Bridgham , Timothy Hunkapillar , Charles Vann
CPC分类号： C12Q1/6869 , B01F13/0059 , B01F13/0222 , B01J19/0046 , B01J2219/00317 , B01J2219/00466 , B01J2219/00527 , B01J2219/00576 , B01J2219/00596 , B01J2219/00648 , B01J2219/00659 , B01J2219/00722 , B01L3/502707 , B01L3/50273 , B01L2200/027 , B01L2200/0647 , B01L2200/0684 , B01L2200/16 , B01L2300/0819 , B01L2300/0822 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2300/1822 , B01L2400/0427 , C12Q1/6874 , G01N35/00069 , G01N35/1002 , G01N35/1097 , G01N2035/00148 , G01N2035/00237 , G01N2035/1034 , G02B21/34 , Y10T436/11 , Y10T436/143333
摘要： According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.
摘要翻译： 根据各种实施方案，提供了一种方法，其包括用洗涤溶液洗涤基底上的DNA包被的珠粒阵列以从基底去除堆叠的珠粒。 洗涤溶液可以在载体中包括惰性固体珠。 DNA包被的珠可以具有平均直径，并且洗涤溶液中的固体珠的平均直径可以是DNA包被的珠的直径的至少两倍。 洗涤可以形成脱落的DNA包被的珠粒和单层DNA包被的珠粒。 在一些实施方案中，用于形成阵列的第一珠粒与包含分子量约350Da或更低的PEG的聚（乙二醇）（PEG）溶液接触。 在一些实施例中，用于形成珠阵列的滑块被提供为用于对其进行成像的系统。
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公开(公告)号：US20110206578A1
公开(公告)日：2011-08-25
申请号：US13033206
申请日：2011-02-23
申请人： Evan Foster , Jessica Reed , Heather Shepherd , Christina Inman , Scott Benson
发明人： Evan Foster , Jessica Reed , Heather Shepherd , Christina Inman , Scott Benson
CPC分类号： C12Q1/6834 , B01J19/0046 , B01J2219/00612 , B01J2219/00635 , B01J2219/00637 , B01J2219/00648 , B01J2219/00722 , B01L3/502707
摘要： Methods for activating and/or reactivating a metal oxide surface for deposition, immobilizing, and/or growing a biological sample (e.g., an oligonucleotide, a polynucleotide, functionalized particle, a polymer, etc.) thereon are disclosed. The metal oxide surface (e.g., zirconium oxide) can be activated and/or reactivated by exposing the metal oxide surface to various activation and/or treatment protocols capable of enhancing a slide's activity relative to the activity of an untreated slide. The activation and/or treatment protocol can include subjecting the slide (or at least the metal oxide layer) to an oxygen plasma treatment and/or a peroxide solution for an amount of time sufficient to activate and/or reactivate the metal oxide surface. Activated sequencing slides capable of incorporation, for example, into various embodiments of a flow cell for use in NGS platforms are also disclosed herein.
摘要翻译： 公开了用于活化和/或重新激活用于在其上沉积，固定和/或生长生物样品（例如寡核苷酸，多核苷酸，官能化颗粒，聚合物等）的金属氧化物表面的方法。 金属氧化物表面（例如，氧化锆）可以通过将金属氧化物表面暴露于能够相对于未处理载玻片的活性增强载玻片的活性的各种活化和/或处理方案而被活化和/或再活化。 活化和/或处理方案可以包括使载玻片（或至少金属氧化物层）经历氧等离子体处理和/或过氧化物溶液足以活化和/或重新活化金属氧化物表面的时间量。 本文还公开了能够并入例如用于NGS平台的流动池的各种实施方案中的活化测序载玻片。
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