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    Automated polypeptide synthesis process
    6.
    发明授权
    Automated polypeptide synthesis process 失效
    自动多肽合成过程

    公开(公告)号：US4816513A

    公开(公告)日：1989-03-28

    申请号：US53324

    申请日：1987-05-22

    申请人： John Bridgham ,  Timothy G. Geiser ,  Michael W. Hunkapiller ,  Stephen B. H. Kent ,  Mark P. Marriott ,  Paul O. Ramstad ,  Eric S. Nordman

    发明人： John Bridgham ,  Timothy G. Geiser ,  Michael W. Hunkapiller ,  Stephen B. H. Kent ,  Mark P. Marriott ,  Paul O. Ramstad ,  Eric S. Nordman

    IPC分类号： C07K1/00 ,  B01J19/00 ,  C07K1/04 ,  C40B40/10 ,  C40B60/14 ,  C08L89/00 ,  C07C103/52

    CPC分类号： C07K1/045 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00389 ,  B01J2219/00484 ,  B01J2219/00488 ,  B01J2219/0049 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00725 ,  C40B40/10 ,  C40B60/14

    摘要： An apparatus is provided for automatically constructing a polypeptide of high purity, up to 50 amino acids in length, using only single couplings. The apparatus includes an activation system for receiving protected amino acids, one kind at a time, having a common vessel (an activator vessel) in which to activate each of the amino acids. Also included is a reaction vessel for containing a resin used in solid-phase peptide synthesis for attaching a peptide chain thereto. A transfer system is also provided, which operates under control of a computer, to transfer the activated species from the activation system to the reaction vessel and to transfer amino acids, reagents, gases, and solvents from one part of the apparatus to another. The activator system also includes a temperature controlled concentrator vessel in which an activator solvent is replaced by a coupling solvent to enhance the coupling of the activated species to the peptide chain in the reaction vessel. Also included in the synthesizer system is a vortexer for affecting total washing of materials in the reaction vessel and the reaction vessel itself, an automated peptide resin sampling system, and an autodelivery system for providing individual containers of amino acid to the synthesizer in the order desired in the peptide sequence. A liquid sensor system is also included to monitor transitions between gases and liquids in specific tubes in the synthesizer in order to provide input signals to the computer system for control purposes. The computer system software which controls the operation of the synthesizer is organized according to a series of menus which allows the user of the system to select individual cycles of operation for each vessel in the synthesizer. In addition, an algorithm has been developed which provides for optimum efficiency in the production of a peptide for any given selection of cycles.

    摘要翻译： 提供了一种用于仅使用单个耦合自动构建高纯度多达50个氨基酸长度的多肽的装置。 该装置包括用于一次接收受保护的氨基酸的激活系统，其具有在其中激活每个氨基酸的共同血管（活化剂容器）。 还包括用于含有固相肽合成中用于连接肽链的树脂的反应容器。 还提供了在计算机控制下操作的转移系统，以将活化物质从活化系统转移到反应容器中，并将氨基酸，试剂，气体和溶剂从设备的一部分转移到另一部分。 活化剂体系还包括温度控制的浓缩容器，其中活化剂溶剂被偶联溶剂代替以增强活化物质与反应容器中的肽链的偶联。 还包括在合成器系统中的是用于影响反应容器和反应容器本身中的材料的全部洗涤的涡流器，自动化肽树脂取样系统和用于按合适的顺序向合成器提供单个氨基酸容器的自动输送系统 在肽序列中。 还包括液体传感器系统以监测合成器中特定管中的气体和液体之间的转换，以便为控制目的向计算机系统提供输入信号。 控制合成器操作的计算机系统软件根据一系列菜单进行组织，这些菜单允许系统的用户为合成器中的每个容器选择各个操作周期。 此外，已经开发了一种算法，其提供用于任何给定选择周期的肽的生产的最佳效率。

    System and apparatus for sequential processing of analytes
    9.
    发明授权
    System and apparatus for sequential processing of analytes 有权
    用于顺序处理分析物的系统和设备

    公开(公告)号：US07282370B2

    公开(公告)日：2007-10-16

    申请号：US11207443

    申请日：2005-08-18

    申请人： John Bridgham ,  Kevin Corcoran ,  George Golda ,  Michael C. Pallas ,  Sydney Brenner

    发明人： John Bridgham ,  Kevin Corcoran ,  George Golda ,  Michael C. Pallas ,  Sydney Brenner

    IPC分类号： G01N21/76 ,  G01N21/00 ,  G01N37/00

    CPC分类号： G01N21/253 ,  B01J2219/00274 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0877 ,  B01L2400/0403 ,  B01L2400/0475 ,  B01L2400/0622 ,  B01L2400/0644 ,  G01N2015/144 ,  G01N2015/1452 ,  G01N2035/00158 ,  Y10S435/81 ,  Y10T436/13 ,  Y10T436/143333

    摘要： An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process.

    摘要翻译： 提供了一种用于同时分析锚定到微粒的多个分析物的装置和系统。 每个具有单一类型分析物的均匀群体的微粒被布置为在流动室内部基本上固定的平面阵列，其中分析过程的步骤通过将一系列处理试剂通过流体系统递送到微粒进行， 微处理器控制。 响应于这样的处理步骤，在每个微粒的表面产生光信号，其特征在于微粒携带的分析物与递送的处理试剂之间的相互作用。 通过收集和记录由平面阵列中的所有微粒产生的光学信号的图像来同时分析多个分析物。 本发明的一个关键特征是在分析过程中由平面阵列中的每个微粒产生的光信号序列的相关性。