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28 条记录 (耗时 0.036 秒)

    Process for purification of polypeptide using a buchner funnel
    3.
    发明授权
    Process for purification of polypeptide using a buchner funnel 失效
    使用布氏漏斗纯化多肽的方法

    公开(公告)号：US5612454A

    公开(公告)日：1997-03-18

    申请号：US776272

    申请日：1991-11-29

    申请人： Toshihiko Kaminuma ,  Toshii Iida ,  Masahiro Tajima

    发明人： Toshihiko Kaminuma ,  Toshii Iida ,  Masahiro Tajima

    IPC分类号： C07K1/16 ,  C07K1/36 ,  A61K38/00 ,  A61K38/02 ,  C07K1/00 ,  C07K5/00

    CPC分类号： C07K1/16 ,  C07K1/36

    摘要： An improved process for purifying a polypeptide using a packing material for reversed phase high performance liquid chromatography is provided. A process for purifying a polypeptide characterized in that an aqueous solution containing polypeptide obtained by pre-treating a polypeptide produced by a wide variety of cells to a predetermined state is adjusted to a specific pH value, to remove impurities, and is then treated with a packing material for reversed phase high performance liquid chromatography.

    摘要翻译： PCT No.PCT / JP91 / 00421 Sec。 371日期：1991年11月29日 102（e）1991年11月29日PCT PCT 1991年3月29日PCT。 公开号WO91 / 15502 日期1991年10月17日提供了一种使用反相高效液相色谱法的填充材料纯化多肽的改进方法。 一种纯化多肽的方法，其特征在于，将通过将由多种细胞产生的多肽预处理至预定状态而获得的含有多肽的水溶液调节至特定的pH值以除去杂质，然后用 用于反相高效液相色谱的包装材料。

    Phenylacetonitrile derivatives and hair tonics and external preparations for skin containing the derivatives
    6.
    发明申请
    Phenylacetonitrile derivatives and hair tonics and external preparations for skin containing the derivatives 审中-公开
    苯乙腈衍生物和头发滋养物以及含有衍生物的皮肤外用制剂

    公开(公告)号：US20050176822A1

    公开(公告)日：2005-08-11

    申请号：US10509246

    申请日：2003-03-28

    申请人： Naoto Hanyu ,  Koji Kobayashi ,  Masahiro Tajima ,  Akihiro Ishino

    发明人： Naoto Hanyu ,  Koji Kobayashi ,  Masahiro Tajima ,  Akihiro Ishino

    IPC分类号： A61K8/40 ,  A61K8/41 ,  A61P17/14 ,  A61Q7/00 ,  A61Q19/00 ,  C07C255/42 ,  C07C255/43 ,  A61K31/277

    CPC分类号： A61Q19/00 ,  A61K8/40 ,  A61K8/41 ,  A61Q7/00 ,  C07C255/42 ,  C07C255/43

    摘要： A hair growth promoting composition comprising, as an effective ingredient, a phenylacetonitrile derivative or a pharmacologically acceptable salt thereof expressed by the following Formula (I): wherein each of R1 and R2 is hydrogen atom, a C1-10 alkyl group, a C2-10 alkenyl group or C2-10 acyl group, or NR1R2 may be a heterocycle having 3-7 members; R3 is a C1-5 alkyl group; and each of R4, R5 and R6 is hydrogen atom or a C1-4 alkoxy group. The present invention provides a hair growth promoting composition having excellent effects on promoting hair regrowth, preventing or inhibiting hair loss, and the like in human by compounding above-specified phenylacetonitrile derivative as a effective ingredient thereto.

    摘要翻译： 一种毛发生长促进组合物，其包含由下式（I）表示的苯乙腈衍生物或其药理学上可接受的盐作为有效成分：其中R 1和R 2各自表示， SUP>是氢原子，C 1-10烷基，C 2-10链烯基或C 2-10酰基， 或NR 1 R 2可以是具有3-7个成员的杂环; R 3是C 1-5烷基; R 4，R 5和R 6中的每一个为氢原子或C 1-4烷氧基 。 本发明提供一种通过将上述规定的苯乙腈衍生物作为有效成分配合而具有对促进毛发再生，预防或抑制脱发等的效果的毛发生长促进组合物。

    Method of preparing an enzyme participating in C-terminal amidation
    7.
    发明授权
    Method of preparing an enzyme participating in C-terminal amidation 失效
    制备参与C-末端酰胺化的酶的方法

    公开(公告)号：US6156555A

    公开(公告)日：2000-12-05

    申请号：US172120

    申请日：1998-10-14

    申请人： Toshii Iida ,  Toshihiko Kaminuma ,  Yuka Fuse ,  Masahiro Tajima ,  Mitsuo Yanagi ,  Hiroshi Okamoto ,  Jiro Kishimoto ,  Ohji Ifuku ,  Ichiro Kato

    发明人： Toshii Iida ,  Toshihiko Kaminuma ,  Yuka Fuse ,  Masahiro Tajima ,  Mitsuo Yanagi ,  Hiroshi Okamoto ,  Jiro Kishimoto ,  Ohji Ifuku ,  Ichiro Kato

    IPC分类号： C07K1/107 ,  C12N9/02 ,  C12N15/53 ,  C12N15/60 ,  C12N9/48 ,  C12N9/14 ,  C12N9/78 ,  C12N9/80 ,  C12N15/00

    CPC分类号： C12N9/0071 ,  C07K1/107 ,  C12Y114/17003 ,  Y10S435/814 ,  Y10S435/815

    摘要： A purified enzyme-I is obtained that participates in C-terminal amidation by acting on a peptide C-terminal glycine adduct to form a peptide C-terminal .alpha.-hydroxyglycine adduct. The enzyme has an optimum pH of about 5 to 7, an optimum temperature of 25 to 40.degree. C. and a molecular weight of about 25 kDa or about 36 kDa, and metal ions and ascorbic acid act as a cofactor. A purified enzyme-II is obtained that participates in C-terminal amidation by acting on the peptide C-terminal .alpha.-hydroxyglycine adduct to produce a C-terminal amidated compound. The enzyme has an optimum pH of about 5 to 6, an optimum temperature of 15 to 35.degree. C. and a molecular weight of about 40 kDa or about 43 kDa. Enzyme-I does not act on the peptide C-terminal .alpha.-hydroxyglycine adduct and enzyme-II does not act on the peptide C-terminal glycine adduct. The enzymes may be purified from a biological material such as horse serum by affinity chromatography using a peptide C-terminal glycine adduct as a ligand. The enzymes may also be obtained from host cells transformed with a plasmid containing a cDNA coding for the enzymes. Assay of activity of the enzymes is carried out by measuring the C-terminal .alpha.-hydroxyglycine adduct or the C-terminal amidated compound that has been isolated such as by high performance liquid chromatography with the use of an acetonitrile-containing buffer.

    摘要翻译： 获得通过作用于肽C-末端甘氨酸加合物以形成肽C末端α-羟基甘氨酸加合物参与C-末端酰胺化的纯化的酶I. 酶的最佳pH为约5至7，最适温度为25至40℃，分子量为约25kDa或约36kDa，金属离子和抗坏血酸用作辅因子。 获得了通过作用于肽C-末端α-羟基甘氨酸加合物参与C-末端酰胺化产生C末端酰胺化合物的纯化的酶-II。 酶的最佳pH为约5至6，最适温度为15至35℃，分子量为约40kDa或约43kDa。 酶-I不对肽C-端α-羟基甘氨酸加合物起作用，酶-II不对肽C-末端甘氨酸加合物起作用。 可以使用肽C-末端甘氨酸加合物作为配体通过亲和层析从生物材料如马血清中纯化酶。 酶也可以从用含有编码酶的cDNA的质粒转化的宿主细胞获得。 酶的活性测定是通过使用含有乙腈的缓冲液，通过高分辨液相色谱法测定已分离的C末端α-羟基甘氨酸加成物或C末端酰胺化合物进行的。