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    Method of manufacturing sugars by trehalase
    6.
    发明授权
    Method of manufacturing sugars by trehalase 失效
    通过海藻糖酶制造糖的方法

    公开(公告)号：US5593869A

    公开(公告)日：1997-01-14

    申请号：US474703

    申请日：1995-06-07

    申请人： Sumio Kitahata ,  Hirofumi Nakano ,  Tsutomu Washino ,  Masamitsu Moriwaki

    发明人： Sumio Kitahata ,  Hirofumi Nakano ,  Tsutomu Washino ,  Masamitsu Moriwaki

    IPC分类号： C12N1/12 ,  C12N9/24 ,  C12P19/12 ,  C12R1/89

    CPC分类号： C12P19/12 ,  C12N9/2402 ,  C12Y302/01028 ,  Y10S435/946

    摘要： Novel trehalase having the properties that it hydrolyzes .alpha.,.alpha.'-trehalose, 2,2'-dideoxy-.alpha.,.alpha.'-trehalose and 2-deoxy-.alpha.,.alpha.'-trehalose into the respective constituting sugars while it does not act on neotrehalose, lactose, maltose, celobiose and sucrose; its optimum pH is 5 to 6; its optimum temperature is 65.degree. C.; it is stable against heating up to 65.degree. C.; its molecular weight as measured by a gel filtration is 400,000 to 500,000 while the molecular weight of the subunit as measured by a sodium dodecylsulfate--polyacrylamide gel electrophoresis is 180,000 to 250,000; its isoelectric point as measured by an isoelectricfocusing is 2.7; and it is a glycoprotein.

    摘要翻译： 具有将α，α'-海藻糖，2,2'-二脱氧-α，α'-海藻糖和2-脱氧-α，α'-海藻糖水解成各自组成糖的性质的新型海藻糖酶，同时不起作用 新海藻糖，乳糖，麦芽糖，头孢噻吩和蔗糖; 其最适pH为5〜6; 其最适温度为65℃。 对于加热至65℃是稳定的; 其通过凝胶过滤测量的分子量为40万至500,000，而通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳测量的亚基的分子量为18万至25万; 通过等电聚焦测量的等电点为2.7; 它是糖蛋白。

    Method for manufacturing alpha-glycosylisoquercitrin, intermediate product and by-product thereof
    7.
    发明申请
    Method for manufacturing alpha-glycosylisoquercitrin, intermediate product and by-product thereof 有权
    制备α-糖基异槲皮素的方法，其中间产物及其副产物

    公开(公告)号：US20080187622A1

    公开(公告)日：2008-08-07

    申请号：US10740607

    申请日：2003-12-22

    申请人： Masamitsu Moriwaki ,  Kazuhiro Emura ,  Hisashi Tanaka

    发明人： Masamitsu Moriwaki ,  Kazuhiro Emura ,  Hisashi Tanaka

    IPC分类号： C11B5/00

    CPC分类号： C12P19/60 ,  A23L5/41 ,  A23L33/105 ,  A61K31/352 ,  A61K31/7048 ,  C12P17/06

    摘要： The present invention provides a method for producing isoquercitrin, α-glycosylisoquercitrin, and rhamnose, the method comprising a step of naringin-degrading enzyme treatment during the isoquercitrin production from rutin in the presence of an edible component, such as gelatin, wheat gluten, chitosan, lecithin, a glycerol fatty acid ester, xanthan gum, carrageenan, sodium chondroitin sulfate, casein, enzymatically decomposed gelatin, sodium alginate, konjac extract, gellan gum, guar gum, soybean protein, agar, pectin, yeast extract, egg-white peptide, cluster dextrin, gum arabic, arginine, sodium metaphosphate, karaya gum, locust bean gum, sodium pyrophosphate, glucosamine, chitin, sodium glutamate, dextrin, trehalose, or a grain-based food ingredients. According to the present invention, isoquercitrin and α-glycosylisoquercitrin, which are of use as antioxidants, anti-fading agents, flavor change inhibitors, etc., can be produced in enhanced yields.

    摘要翻译： 本发明提供异槲皮素，α-糖基异槲皮苷和鼠李糖的制备方法，该方法包括在可食用成分如明胶，小麦面筋，壳聚糖存在下，从芦丁生产异槲皮苷期间柚苷降解酶处理的步骤 ，卵磷脂，甘油脂肪酸酯，黄原胶，角叉菜胶，硫酸软骨素钠，酪蛋白，酶分解明胶，藻酸钠，魔芋提取物，结冷胶，瓜尔胶，大豆蛋白，琼脂，果胶，酵母提取物，蛋白肽 ，糊精，阿拉伯树胶，精氨酸，偏磷酸钠，刺槐豆胶，刺槐豆胶，焦磷酸钠，葡糖胺，甲壳素，谷氨酸钠，糊精，海藻糖或谷物类食品成分。 根据本发明，可以以增加的产率制备用作抗氧化剂的异槲皮素和α-糖基异槲皮素，抗褪色剂，风味变化抑制剂等。

    Method for manufacturing α-glycosylisoquercitrin, intermediate product and by-product thereof
    8.
    发明授权
    Method for manufacturing α-glycosylisoquercitrin, intermediate product and by-product thereof 有权
    α-糖基异槲皮素的制备方法，其中间产物及其副产物

    公开(公告)号：US07691425B2

    公开(公告)日：2010-04-06

    申请号：US10740607

    申请日：2003-12-22

    申请人： Masamitsu Moriwaki ,  Kazuhiro Emura ,  Hisashi Tanaka

    发明人： Masamitsu Moriwaki ,  Kazuhiro Emura ,  Hisashi Tanaka

    IPC分类号： A23L1/00

    CPC分类号： C12P19/60 ,  A23L5/41 ,  A23L33/105 ,  A61K31/352 ,  A61K31/7048 ,  C12P17/06

    摘要： The present invention provides a method for producing isoquercitrin, α-glycosylisoquercitrin, and rhamnose, the method comprising a step of naringin-degrading enzyme treatment during the isoquercitrin production from rutin in the presence of an edible component, such as gelatin, wheat gluten, chitosan, lecithin, a glycerol fatty acid ester, xanthan gum, carrageenan, sodium chondroitin sulfate, casein, enzymatically decomposed gelatin, sodium alginate, konjac extract, gellan gum, guar gum, soybean protein, agar, pectin, yeast extract, egg-white peptide, cluster dextrin, gum arabic, arginine, sodium metaphosphate, karaya gum, locust bean gum, sodium pyrophosphate, glucosamine, chitin, sodium glutamate, dextrin, trehalose, or a grain-based food ingredients. According to the present invention, isoquercitrin and α-glycosylisoquercitrin, which are of use as antioxidants, anti-fading agents, flavor change inhibitors, etc., can be produced in enhanced yields.

    摘要翻译： 本发明提供异槲皮素，α-糖基异槲皮素和鼠李糖的制造方法，其特征在于，在可食用成分如明胶，小麦面筋，壳聚糖等存在下，由芦丁生产异槲皮素期间柚皮苷降解酶处理步骤 ，卵磷脂，甘油脂肪酸酯，黄原胶，角叉菜胶，硫酸软骨素钠，酪蛋白，酶分解明胶，藻酸钠，魔芋提取物，结冷胶，瓜尔胶，大豆蛋白，琼脂，果胶，酵母提取物，蛋白肽 ，糊精，阿拉伯树胶，精氨酸，偏磷酸钠，刺槐豆胶，刺槐豆胶，焦磷酸钠，葡糖胺，甲壳素，谷氨酸钠，糊精，海藻糖或谷物类食品成分。 根据本发明，可以以增加的产率生产用作抗氧化剂的异槲皮素和α-糖基异槲皮素，抗褪色剂，风味变化抑制剂等。

    Quercetin Glycoside Composition and Method of Preparing the Same
    9.
    发明申请
    Quercetin Glycoside Composition and Method of Preparing the Same 审中-公开
    槲皮素糖苷组合物及其制备方法

    公开(公告)号：US20090143317A1

    公开(公告)日：2009-06-04

    申请号：US11794218

    申请日：2005-12-28

    申请人： Yoshiko Ono ,  Namino Tomimori ,  Norifumi Tateishi ,  Masamitsu Moriwaki ,  Kazuhiro Emura ,  Shuji Okuyama

    发明人： Yoshiko Ono ,  Namino Tomimori ,  Norifumi Tateishi ,  Masamitsu Moriwaki ,  Kazuhiro Emura ,  Shuji Okuyama

    IPC分类号： A61K31/7048 ,  C12P19/60

    CPC分类号： C12P19/22 ,  A23L3/3463 ,  A23L3/3562 ,  A23L33/105 ,  A23V2002/00 ,  A61K31/7048 ,  C12P19/60 ,  A61K2300/00 ,  A23V2200/02 ,  A23V2250/21168

    摘要： The present invention provides an α-glycosyl isoquercitrin-containing novel composition which has a high in vivo absorbability, and hence exhibits a significant in vivo antioxidative activity. The present invention further provides preparation methods for such a composition. The composition contains a mixture of quercetin glycosides represented by the following formula: wherein Glc represents a glucose residue, and n is 0 or a positive integer of 1 or more,includes at least a quercetin glycoside wherein n is 3, and satisfies the following requirement (a):(a) the total proportion of quercetin glycosides in which n is 3, and in which other n values may be 1 or 2, or 1 and 2, is 50 mol % or more, and the total proportion of quercetin glycosides wherein n is 4 or more is 15 mol % or less, in the composition. The composition can be prepared by treating an enzymatically modified isoquercitrin with β-amylase.

    摘要翻译： 本发明提供了具有高体内吸收性的α-糖基异槲皮苷新化合物，因此具有显着的体内抗氧化活性。 本发明还提供了这种组合物的制备方法。 该组合物含有由下式表示的槲皮素糖苷的混合物：其中，Glc表示葡萄糖残基，n为0或1以上的正整数，至少包含槲皮素苷，n为3，满足以下要求 （a）：（a）其中n为3，其它n值可以为1或2，或1和2的槲皮素苷的总比例为50摩尔％以上，槲皮素苷的总比例 n为4以上15摩尔％以下。 可以通过用β-淀粉酶处理酶促修饰的异槲皮素来制备组合物。