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    IN VITRO METHOD TO DETERMINE WHETHER A DRUG CANDIDATE ACTIVE AGAINST A TARGET PROTEIN IS ACTIVE AGAINST A VARIANT OF SAID PROTEIN
    1.
    发明申请
    IN VITRO METHOD TO DETERMINE WHETHER A DRUG CANDIDATE ACTIVE AGAINST A TARGET PROTEIN IS ACTIVE AGAINST A VARIANT OF SAID PROTEIN 审中-公开
    用于确定与目标蛋白有关的药物候选药物的体外方法是否对一种不同的蛋白质有效

    公开(公告)号：US20110251085A1

    公开(公告)日：2011-10-13

    申请号：US12922409

    申请日：2009-03-16

    申请人： Mehdi Lahmar ,  Isabelle Valarche ,  Majid Mehtali

    发明人： Mehdi Lahmar ,  Isabelle Valarche ,  Majid Mehtali

    IPC分类号： C40B30/04

    CPC分类号： G01N33/5008 ,  G01N33/5767 ,  G01N33/6845 ,  G01N2333/16 ,  G01N2333/70567

    摘要： The present invention relates to the field of virology. More precisely, the invention provides a method of determining the ability of a test compound to modulate the biological activity of a variant of a target protein, wherein said test compound is previously known to modulate the biological activity of said protein. This invention is useful to determine whether a drug candidate, such as anti-viral compounds (eg. against hepatitis C virus: NS5B, NS3), active against a target protein is active against a variant of said protein (eg. polymorphisms, genotypes or mutants).

    摘要翻译： 本发明涉及病毒学领域。 更准确地说，本发明提供了测定化合物调节靶蛋白变体的生物学活性的能力的方法，其中所述测试化合物以前已知可以调节所述蛋白质的生物活性。 本发明可用于确定针对靶蛋白的抗病毒化合物（例如针对丙型肝炎病毒：NS5B，NS3）的药物候选物是否对所述蛋白质的变体（例如多态性，基因型或 突变体）。

    Method of obtaining chicken embryonic stem cells
    2.
    发明授权
    Method of obtaining chicken embryonic stem cells 有权
    获得鸡胚胎干细胞的方法

    公开(公告)号：US08962311B2

    公开(公告)日：2015-02-24

    申请号：US12376660

    申请日：2007-08-09

    申请人： Isabelle Valarche ,  Luc Batard ,  Majid Mehtali ,  Fabienne Guehenneux

    发明人： Isabelle Valarche ,  Luc Batard ,  Majid Mehtali ,  Fabienne Guehenneux

    IPC分类号： C12N5/00 ,  A01K67/027

    CPC分类号： C12N5/0606 ,  A01K2217/00 ,  A01K2227/30 ,  C12N2501/10 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/23 ,  C12N2501/2306 ,  C12N2502/13

    摘要： Method of culturing embryonic stem (ES) cells of avian origin includes the steps of: a) suspending ES cells originating from the blastoderm disk of fertilized un-incubated avian egg(s) in a basal culture medium supplemented with: insulin-like growth factor-1 (IGF-1) and ciliary neurotrophic factor (CNTF); and animal serum; and, optionally, at least one growth factor selected from among interleukin 6 (Il-6), interleukin 6 receptor (Il-6R), stem cell factor (SCF), fibroblast growth factor (FGF), leukemia inhibitory factor (LIF), interleukin 11 (Il-11), oncostatin and/or cardiotrophin; b) seeding the suspension of ES cells obtained in step a) on a layer of feeder cells and further culturing the ES cells for at least 2 to 10 passages; c) optionally, removing at least one growth factor selected from among SCF, FGF, Il-6, Il-6R, LIF, oncostatin, cardiotrophin and Il-11 from the culture medium; and d) further culturing the ES cells in the medium of step c) on a layer of feeder cells.

    摘要翻译： 培养禽源性胚胎干（ES）细胞的方法包括以下步骤：a）将源自受精的未孵化的禽类卵的胚盘的ES细胞悬浮在补充有胰岛素样生长因子的基础培养基中 -1（IGF-1）和睫状神经营养因子（CNTF）; 和动物血清; 和任选的至少一种选自白介素6（II-6），白介素6受体（II-6R），干细胞因子（SCF），成纤维细胞生长因子（FGF），白血病抑制因子（LIF） 白介素11（Il-11），制瘤素和/或心肌营养素; b）将步骤a）中获得的ES细胞的悬浮液接种在饲养细胞层上，并进一步培养ES细胞至少2至10代; c）任选地，从培养基中除去选自SCF，FGF，II-6，II-6R，LIF，制瘤素，心肌营养素和II-11中的至少一种生长因子; 和d）在步骤c）的培养基中进一步培养ES细胞在饲养细胞层上。

    PRODUCTION OF TRANSGENIC AVIAN ORGANISMS EMPLOYING EMBRYONIC STEM CELLS
    3.
    发明申请
    PRODUCTION OF TRANSGENIC AVIAN ORGANISMS EMPLOYING EMBRYONIC STEM CELLS 有权
    生产使用胚胎干细胞的转基因生物有机体

    公开(公告)号：US20100235937A1

    公开(公告)日：2010-09-16

    申请号：US12376660

    申请日：2007-08-09

    申请人： Isabelle Valarche ,  Luc Batard ,  Majid Mehtali

    发明人： Isabelle Valarche ,  Luc Batard ,  Majid Mehtali

    IPC分类号： A01K67/00 ,  C12N5/10 ,  C12Q1/68

    CPC分类号： C12N5/0606 ,  A01K2217/00 ,  A01K2227/30 ,  C12N2501/10 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/13 ,  C12N2501/23 ,  C12N2501/2306 ,  C12N2502/13

    摘要： Method of culturing embryonic stem (ES) cells of avian origin includes the steps of: a) suspending ES cells originating from the blastoderm disk of fertilized un-incubated avian egg(s) in a basal culture medium supplemented with: insulin-like growth factor-1 (IGF-1) and ciliary neurotrophic factor (CNTF); and animal serum; and, optionally, at least one growth factor selected from among interleukin 6 (II-6), interleukin 6 receptor (II-6R), stem cell factor (SCF), fibroblast growth factor (FGF), leukemia inhibitory factor (LIF), interleukin 11 (II-11), oncostatin and/or cardiotrophin;b) seeding the suspension of ES cells obtained in step a) on a layer of feeder cells and further culturing the ES cells for at least 2 to 10 passages; c) optionally, removing at least one growth factor selected from among SCF, FGF, II-6, II-6R, LIF, oncostatin, cardiotrophin and II-11 from the culture medium; and d) further culturing the ES cells in the medium of step c) on a layer of feeder cells.

    摘要翻译： 培养禽源性胚胎干（ES）细胞的方法包括以下步骤：a）将源自受精的未孵化的禽类卵的胚盘的ES细胞悬浮在补充有胰岛素样生长因子的基础培养基中 -1（IGF-1）和睫状神经营养因子（CNTF）; 和动物血清; 和选自白细胞介素6（II-6），白细胞介素6受体（II-6R），干细胞因子（SCF），成纤维细胞生长因子（FGF），白血病抑制因子（LIF） 白细胞介素11（II-11），制瘤素和/或心肌营养素; b）将步骤a）中获得的ES细胞的悬浮液接种在饲养细胞层上，并进一步培养ES细胞至少2至10代; c）任选地，从培养基中除去选自SCF，FGF，II-6，II-6R，LIF，制瘤素，心肌营养素和II-11中的至少一种生长因子; 和d）在步骤c）的培养基中进一步培养ES细胞在饲养细胞层上。

    Chimeric promoters for controlling expression in smooth muscle cells
    5.
    发明授权
    Chimeric promoters for controlling expression in smooth muscle cells 失效
    用于控制平滑肌细胞中表达的嵌合启动子

    公开(公告)号：US07482155B1

    公开(公告)日：2009-01-27

    申请号：US10312963

    申请日：2001-07-04

    申请人： Sébastien Ribault ,  Pascal Neuville ,  Majid Mehtali

    发明人： Sébastien Ribault ,  Pascal Neuville ,  Majid Mehtali

    IPC分类号： C12N15/63 ,  A61K48/00 ,  C07H21/04

    CPC分类号： C12N15/85 ,  A61K48/00 ,  A61K48/0058 ,  C07K14/4716 ,  C12N9/1223 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2799/022 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/85

    摘要： The present invention concerns a chimeric construct comprising a SMC-specific promoter operably linked to a muscle-specific enhancer. It also provides an expression cassette comprising such a chimeric construct to control expression of a therapeutic gene. Finally, the invention relates to a recombinant vector, a viral particle, an eukaryotic host cell, a composition comprising said expression cassette and their use for specific expression in SMCs and for therapeutic or prophylactic purposes, a method for the treatment of a human or animal organism as well as a transgenic non-human animal comprising integrated into its genome the chimeric construct, the expression cassette or the vector of the present invention.

    摘要翻译： 本发明涉及包含与肌肉特异性增强子可操作地连接的SMC特异性启动子的嵌合构建体。 它还提供了包含这种嵌合构建体以控制治疗基因表达的表达盒。 最后，本发明涉及重组载体，病毒颗粒，真核宿主细胞，包含所述表达盒的组合物及其在SMC中特异性表达和用于治疗或预防目的的用途，用于治疗人或动物的方法 生物体以及包含在其基因组中整合到本发明的嵌合构建体，表达盒或载体中的转基因非人动物。

    Complementing cell lines
    6.
    发明授权
    Complementing cell lines 有权
    补充细胞系

    公开(公告)号：US07344883B2

    公开(公告)日：2008-03-18

    申请号：US11165697

    申请日：2005-06-24

    申请人： Ronald Vogels ,  Menzo Jans Emco Havenga ,  Majid Mehtali

    发明人： Ronald Vogels ,  Menzo Jans Emco Havenga ,  Majid Mehtali

    IPC分类号： C12N5/10 ,  C12N5/08 ,  C12N7/00 ,  C12N15/861

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N7/025 ,  C12N15/861 ,  C12N2710/10041 ,  C12N2710/10051 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2840/20

    摘要： A packaging cell line that complements recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably derived from primary, diploid human cells that are transformed by adenovirus E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules, the sequences being operatively linked to regulatory sequences enabling transcription and translation of encoded proteins. Also disclosed is a cell line derived from PER.C6 that expresses functional Ad35 E1B sequences. The Ad35-E1B sequences are driven by the E1B promoter or a heterologous promoter and terminated by a heterologous poly-adenylation signal. The cell lines are useful for producing recombinant adenoviruses designed for gene therapy and vaccination. The cell lines can also be used for producing human recombinant therapeutic proteins such as human growth factors and human antibodies. Also, the cell lines are useful for producing human viruses other than adenovirus such as influenza virus, herpes simplex virus, rotavirus, and measles virus.

    摘要翻译： 一种包装细胞系，其基于来自亚组B，优选腺病毒35的血清型补充重组腺病毒。细胞系优选衍生自通过腺病毒E1序列转化的原代二倍体人细胞，所述腺病毒E1序列可操作地连接在一个DNA分子上或位于两个 分离的DNA分子，该序列可操作地连接到能够转录和翻译编码蛋白质的调控序列。 还公开了衍生自表达功能性Ad35 E1B序列的PER.C6的细胞系。 Ad35-E1B序列由E1B启动子或异源启动子驱动，并通过异源多聚腺苷酸化信号终止。 细胞系可用于生产设计用于基因治疗和疫苗接种的重组腺病毒。 细胞系也可以用于生产人重组治疗蛋白质，例如人生长因子和人抗体。 此外，细胞系可用于生产除了腺病毒如流感病毒，单纯疱疹病毒，轮状病毒和麻疹病毒之外的人类病毒。

    Complementing cell lines
    7.
    发明授权
    Complementing cell lines 有权
    补充细胞系

    公开(公告)号：US06869794B2

    公开(公告)日：2005-03-22

    申请号：US10164085

    申请日：2002-06-04

    申请人： Ronald Vogels ,  Menzo Havenga ,  Majid Mehtali

    发明人： Ronald Vogels ,  Menzo Havenga ,  Majid Mehtali

    IPC分类号： C12N15/09 ,  A61K48/00 ,  C12N5/02 ,  C12N5/08 ,  C12N5/10 ,  C12N7/00 ,  C12N7/01 ,  C12N7/02 ,  C12N15/00 ,  C12N15/35 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/861 ,  C12P1/00 ,  C12P21/06

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N7/025 ,  C12N15/861 ,  C12N2710/10041 ,  C12N2710/10051 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2840/20

    摘要： A packaging cell line capable of complementing recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably derived from primary, diploid human cells transformed by adenovirus E1 sequences either operatively linked on one or two DNA molecules, the sequences operatively linked to regulatory sequences enabling transcription and translation of encoded proteins. Also, a cell line derived from PER.C6 that expresses functional Ad35 E1B sequences. The Ad35-E1B sequences are driven by the E1B promoter and terminated by a heterologous poly-adenylation signal. The new cell lines are useful for producing recombinant adenoviruses. The cell lines can be used to produce human recombinant therapeutic proteins such as human antibodies. In addition, the cell lines are useful for producing human viruses other than adenovirus such as influenza, herpes simplex, rotavirus, and measles.

    摘要翻译： 能够基于来自亚组B，优选腺病毒35的血清型补充重组腺病毒的包装细胞系。细胞系优选衍生自由一个或两个DNA分子可操作地连接的腺病毒E1序列转化的原代二倍体人细胞，序列 可操作地连接到能够转录和翻译编码蛋白质的调节序列。 此外，衍生自表达功能性Ad35 E1B序列的PER.C6的细胞系。 Ad35-E1B序列由E1B启动子驱动并通过异源多聚腺苷酸化信号终止。 新的细胞系可用于生产重组腺病毒。 细胞系可用于产生人类重组治疗蛋白，如人抗体。 此外，细胞系可用于产生除腺病毒如流感，单纯疱疹，轮状病毒和麻疹以外的人类病毒。