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公开(公告)号:US09068181B2
公开(公告)日:2015-06-30
申请号:US12472346
申请日:2009-05-26
申请人: Jon F. Edd , Mehmet Toner , Dino Dicarlo , Daniel Irimia
发明人: Jon F. Edd , Mehmet Toner , Dino Dicarlo , Daniel Irimia
CPC分类号: C12N11/04
摘要: Microfluidic devices and methods for the encapsulation of particles within liquid droplets are disclosed. The new methods and devices form 1-100 picoliter-size monodisperse droplets containing the particles, such as single cells, encapsulated in individual liquid droplets. The particles can be encapsulated in droplets of a fluid by passing a fluid containing the particles through a high aspect-ratio microchannel to order the particles in the fluid, followed by forming the fluid into droplets. The resulting fraction of the liquid droplets with a single particle (e.g., a cell) is higher than the corresponding fraction of single-particle liquid droplets predicted by Poisson statistics.
摘要翻译: 公开了用于在液滴内包封颗粒的微流体装置和方法。 新的方法和装置形成1-100皮升大小的单分散液滴,其包含单个细胞的颗粒,包封在单个液滴中。 通过使含有颗粒的流体通过高纵横比的微通道将粒子封装在流体的液滴中,以使颗粒在流体中排列,然后将流体形成液滴。 所得到的具有单个颗粒(例如,电池)的液滴的部分高于由泊松统计预测的单颗粒液滴的相应部分。
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公开(公告)号:US08784012B2
公开(公告)日:2014-07-22
申请号:US13480862
申请日:2012-05-25
申请人: Mehmet Toner , Dino DiCarlo , Jon F. Edd , Daniel Irimia
发明人: Mehmet Toner , Dino DiCarlo , Jon F. Edd , Daniel Irimia
IPC分类号: B65G53/52
CPC分类号: B01L3/502776 , B01D21/0087 , B01D21/265 , B01D45/04 , B01D45/12 , B01L3/502746 , B01L3/502761 , B01L2200/0652 , B01L2300/0883 , B01L2400/0409 , B01L2400/043 , B01L2400/0433 , B01L2400/0454 , B01L2400/0487 , B01L2400/0622 , B01L2400/084 , B33Y80/00 , C12M23/16 , C12M41/36 , C12M47/04 , F16L41/02 , F17D1/00 , G01N15/0255 , G01N15/10 , G01N2015/0288 , G01N2015/1006 , G01N2015/1081 , Y10T137/85938 , Y10T137/85978 , Y10T436/117497 , Y10T436/2575
摘要: Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.
摘要翻译: 提供了各种系统,方法和装置,用于将悬浮在移动流体中的颗粒聚焦到一个或多个局部流线中。 该系统可以包括基底和设置在基底上的至少一个通道,其具有入口和出口。 该系统还可以包括在具有悬浮颗粒的层流中沿着通道移动的流体和驱动流体层流的泵送元件。 流体,通道和泵送元件可以被配置成使惯性力作用在颗粒上并将颗粒聚焦成一条或多条流线。
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公开(公告)号:US20100021984A1
公开(公告)日:2010-01-28
申请号:US12472346
申请日:2009-05-26
申请人: Jon F. Edd , Mehmet Toner , Dino Dicarlo , Daniel Irimia
发明人: Jon F. Edd , Mehmet Toner , Dino Dicarlo , Daniel Irimia
IPC分类号: C12N11/00
CPC分类号: C12N11/04
摘要: Microfluidic devices and methods for the encapsulation of particles within liquid droplets are disclosed. The new methods and devices form 1-100 picoliter-size monodisperse droplets containing the particles, such as single cells, encapsulated in individual liquid droplets. The particles can be encapsulated in droplets of a fluid by passing a fluid containing the particles through a high aspect-ratio microchannel to order the particles in the fluid, followed by forming the fluid into droplets. The resulting fraction of the liquid droplets with a single particle (e.g., a cell) is higher than the corresponding fraction of single-particle liquid droplets predicted by Poisson statistics.
摘要翻译: 公开了用于在液滴内包封颗粒的微流体装置和方法。 新的方法和装置形成1-100皮升大小的单分散液滴,其包含单个细胞的颗粒,包封在单个液滴中。 通过使含有颗粒的流体通过高纵横比的微通道将粒子封装在流体的液滴中,以使颗粒在流体中排列,然后将流体形成液滴。 所得到的具有单个颗粒(例如,电池)的液滴的部分高于由泊松统计预测的单颗粒液滴的相应部分。
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公开(公告)号:US20130011210A1
公开(公告)日:2013-01-10
申请号:US13619309
申请日:2012-09-14
申请人: Mehmet Toner , Dino Dicarlo , Jon F. Edd , Daniel Irimia
发明人: Mehmet Toner , Dino Dicarlo , Jon F. Edd , Daniel Irimia
IPC分类号: B65G53/30
CPC分类号: B01L3/502776 , B01D21/0087 , B01D21/265 , B01D45/04 , B01D45/12 , B01L3/502746 , B01L3/502761 , B01L2200/0652 , B01L2300/0883 , B01L2400/0409 , B01L2400/043 , B01L2400/0433 , B01L2400/0454 , B01L2400/0487 , B01L2400/0622 , B01L2400/084 , B33Y80/00 , C12M23/16 , C12M41/36 , C12M47/04 , F16L41/02 , F17D1/00 , G01N15/0255 , G01N15/10 , G01N2015/0288 , G01N2015/1006 , G01N2015/1081 , Y10T137/85938 , Y10T137/85978 , Y10T436/117497 , Y10T436/2575
摘要: Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.
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公开(公告)号:US08807879B2
公开(公告)日:2014-08-19
申请号:US13619309
申请日:2012-09-14
申请人: Mehmet Toner , Dino Dicarlo , Jon F. Edd , Daniel Irimia
发明人: Mehmet Toner , Dino Dicarlo , Jon F. Edd , Daniel Irimia
IPC分类号: B65G53/00
CPC分类号: B01L3/502776 , B01D21/0087 , B01D21/265 , B01D45/04 , B01D45/12 , B01L3/502746 , B01L3/502761 , B01L2200/0652 , B01L2300/0883 , B01L2400/0409 , B01L2400/043 , B01L2400/0433 , B01L2400/0454 , B01L2400/0487 , B01L2400/0622 , B01L2400/084 , B33Y80/00 , C12M23/16 , C12M41/36 , C12M47/04 , F16L41/02 , F17D1/00 , G01N15/0255 , G01N15/10 , G01N2015/0288 , G01N2015/1006 , G01N2015/1081 , Y10T137/85938 , Y10T137/85978 , Y10T436/117497 , Y10T436/2575
摘要: Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.
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公开(公告)号:US20120292233A1
公开(公告)日:2012-11-22
申请号:US13480862
申请日:2012-05-25
申请人: Mehmet Toner , Dino DiCarlo , Jon F. Edd , Daniel Irimia
发明人: Mehmet Toner , Dino DiCarlo , Jon F. Edd , Daniel Irimia
CPC分类号: B01L3/502776 , B01D21/0087 , B01D21/265 , B01D45/04 , B01D45/12 , B01L3/502746 , B01L3/502761 , B01L2200/0652 , B01L2300/0883 , B01L2400/0409 , B01L2400/043 , B01L2400/0433 , B01L2400/0454 , B01L2400/0487 , B01L2400/0622 , B01L2400/084 , B33Y80/00 , C12M23/16 , C12M41/36 , C12M47/04 , F16L41/02 , F17D1/00 , G01N15/0255 , G01N15/10 , G01N2015/0288 , G01N2015/1006 , G01N2015/1081 , Y10T137/85938 , Y10T137/85978 , Y10T436/117497 , Y10T436/2575
摘要: Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.
摘要翻译: 提供了各种系统,方法和装置,用于将悬浮在移动流体中的颗粒聚焦到一个或多个局部流线中。 该系统可以包括基底和设置在基底上的至少一个通道,其具有入口和出口。 该系统还可以包括在具有悬浮颗粒的层流中沿着通道移动的流体和驱动流体层流的泵送元件。 流体,通道和泵送元件可以被配置成使惯性力作用在颗粒上并将颗粒聚焦成一条或多条流线。
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公开(公告)号:US20090014360A1
公开(公告)日:2009-01-15
申请号:US12103885
申请日:2008-04-16
申请人: Mehmet Toner , Dino DiCarlo , Jon Edd , Daniel Irimia
发明人: Mehmet Toner , Dino DiCarlo , Jon Edd , Daniel Irimia
CPC分类号: B01L3/502776 , B01D21/0087 , B01D21/265 , B01D45/04 , B01D45/12 , B01L3/502746 , B01L3/502761 , B01L2200/0652 , B01L2300/0883 , B01L2400/0409 , B01L2400/043 , B01L2400/0433 , B01L2400/0454 , B01L2400/0487 , B01L2400/0622 , B01L2400/084 , B33Y80/00 , C12M23/16 , C12M41/36 , C12M47/04 , F16L41/02 , F17D1/00 , G01N15/0255 , G01N15/10 , G01N2015/0288 , G01N2015/1006 , G01N2015/1081 , Y10T137/85938 , Y10T137/85978 , Y10T436/117497 , Y10T436/2575
摘要: Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.
摘要翻译: 提供了各种系统,方法和装置,用于将悬浮在移动流体中的颗粒聚焦到一个或多个局部流线中。 该系统可以包括基底和设置在基底上的至少一个通道,其具有入口和出口。 该系统还可以包括在具有悬浮颗粒的层流中沿着通道移动的流体和驱动流体层流的泵送元件。 流体,通道和泵送元件可以被配置成使惯性力作用在颗粒上并将颗粒聚焦成一条或多条流线。
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公开(公告)号:US08186913B2
公开(公告)日:2012-05-29
申请号:US12103885
申请日:2008-04-16
申请人: Mehmet Toner , Dino DiCarlo , Jon Edd , Daniel Irimia
发明人: Mehmet Toner , Dino DiCarlo , Jon Edd , Daniel Irimia
IPC分类号: B65G53/52
CPC分类号: B01L3/502776 , B01D21/0087 , B01D21/265 , B01D45/04 , B01D45/12 , B01L3/502746 , B01L3/502761 , B01L2200/0652 , B01L2300/0883 , B01L2400/0409 , B01L2400/043 , B01L2400/0433 , B01L2400/0454 , B01L2400/0487 , B01L2400/0622 , B01L2400/084 , B33Y80/00 , C12M23/16 , C12M41/36 , C12M47/04 , F16L41/02 , F17D1/00 , G01N15/0255 , G01N15/10 , G01N2015/0288 , G01N2015/1006 , G01N2015/1081 , Y10T137/85938 , Y10T137/85978 , Y10T436/117497 , Y10T436/2575
摘要: Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.
摘要翻译: 提供了各种系统,方法和装置,用于将悬浮在移动流体内的颗粒聚焦到一个或多个局部流线中。 该系统可以包括基底和设置在基底上的至少一个通道,其具有入口和出口。 该系统还可以包括在具有悬浮颗粒的层流中沿着通道移动的流体和驱动流体层流的泵送元件。 流体,通道和泵送元件可以被配置成使惯性力作用在颗粒上并将颗粒聚焦成一条或多条流线。
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公开(公告)号:US08911957B2
公开(公告)日:2014-12-16
申请号:US12293046
申请日:2007-03-15
IPC分类号: G01N33/543 , G01N33/53 , G01N33/569
CPC分类号: G01N33/56972 , G01N33/56966 , Y10S436/824
摘要: The invention features methods, devices, and kits for the isolation of analytes (e.g., a cell). A sample containing a desired analyte is introduced into a microfluidic device containing moieties that bind the desired analyte. A shear stress is applied that is great enough to prevent binding of undesired analytes and low enough to allow binding of the analyte of interest. Once bound, the desired analytes can be analyzed (e.g., counted). The invention also features methods for determining a shear stress for isolating a desired analyte.
摘要翻译: 本发明的特征在于用于分离分析物(例如,细胞)的方法,装置和试剂盒。 将含有所需分析物的样品引入含有结合所需分析物的部分的微流体装置中。 施加的剪切应力足够大以防止不需要的分析物的结合,并且足够低以允许感兴趣的分析物的结合。 一旦结合,可以分析所需的分析物(例如,计数)。 本发明还具有确定用于分离所需分析物的剪切应力的方法。
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公开(公告)号:US08343756B2
公开(公告)日:2013-01-01
申请号:US12089440
申请日:2006-10-10
申请人: Mehmet Toner , Daniel Irimia
发明人: Mehmet Toner , Daniel Irimia
CPC分类号: F16K99/0059 , B01L3/502738 , B01L2200/0668 , B01L2300/0816 , B01L2300/123 , B01L2400/0481 , B01L2400/0487 , B01L2400/0633 , B01L2400/0638 , B01L2400/0677 , C12M47/06 , F16K99/0001 , F16K99/0005 , F16K99/0034 , F16K2099/0074 , F16K2099/0084
摘要: Devices for fluid control and biological particle manipulation (e.g., cell enrichment and blood sampling) are disclosed. The devices a based on the ability to control the flow of fluids through the use of microfluidic valves. The valves are characterized, for example, by microstructures disposed on a mobile diaphragm.
摘要翻译: 公开了用于流体控制和生物粒子操纵(例如细胞富集和血液取样)的装置。 装置a基于通过使用微流体阀控制流体流动的能力。 阀的特征在于例如通过设置在移动隔膜上的微结构。
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