Microfluidic droplet encapsulation
    1.
    发明授权
    Microfluidic droplet encapsulation 有权
    微流体液滴封装

    公开(公告)号:US09068181B2

    公开(公告)日:2015-06-30

    申请号:US12472346

    申请日:2009-05-26

    CPC分类号: C12N11/04

    摘要: Microfluidic devices and methods for the encapsulation of particles within liquid droplets are disclosed. The new methods and devices form 1-100 picoliter-size monodisperse droplets containing the particles, such as single cells, encapsulated in individual liquid droplets. The particles can be encapsulated in droplets of a fluid by passing a fluid containing the particles through a high aspect-ratio microchannel to order the particles in the fluid, followed by forming the fluid into droplets. The resulting fraction of the liquid droplets with a single particle (e.g., a cell) is higher than the corresponding fraction of single-particle liquid droplets predicted by Poisson statistics.

    摘要翻译: 公开了用于在液滴内包封颗粒的微流体装置和方法。 新的方法和装置形成1-100皮升大小的单分散液滴,其包含单个细胞的颗粒,包封在单个液滴中。 通过使含有颗粒的流体通过高纵横比的微通道将粒子封装在流体的液滴中,以使颗粒在流体中排列,然后将流体形成液滴。 所得到的具有单个颗粒(例如,电池)的液滴的部分高于由泊松统计预测的单颗粒液滴的相应部分。

    Microfluidic Droplet Encapsulation
    3.
    发明申请
    Microfluidic Droplet Encapsulation 有权
    微流控液滴包封

    公开(公告)号:US20100021984A1

    公开(公告)日:2010-01-28

    申请号:US12472346

    申请日:2009-05-26

    IPC分类号: C12N11/00

    CPC分类号: C12N11/04

    摘要: Microfluidic devices and methods for the encapsulation of particles within liquid droplets are disclosed. The new methods and devices form 1-100 picoliter-size monodisperse droplets containing the particles, such as single cells, encapsulated in individual liquid droplets. The particles can be encapsulated in droplets of a fluid by passing a fluid containing the particles through a high aspect-ratio microchannel to order the particles in the fluid, followed by forming the fluid into droplets. The resulting fraction of the liquid droplets with a single particle (e.g., a cell) is higher than the corresponding fraction of single-particle liquid droplets predicted by Poisson statistics.

    摘要翻译: 公开了用于在液滴内包封颗粒的微流体装置和方法。 新的方法和装置形成1-100皮升大小的单分散液滴,其包含单个细胞的颗粒,包封在单个液滴中。 通过使含有颗粒的流体通过高纵横比的微通道将粒子封装在流体的液滴中,以使颗粒在流体中排列,然后将流体形成液滴。 所得到的具有单个颗粒(例如,电池)的液滴的部分高于由泊松统计预测的单颗粒液滴的相应部分。

    Devices and methods for detecting cells and other analytes
    9.
    发明授权
    Devices and methods for detecting cells and other analytes 有权
    用于检测细胞和其他分析物的装置和方法

    公开(公告)号:US08911957B2

    公开(公告)日:2014-12-16

    申请号:US12293046

    申请日:2007-03-15

    摘要: The invention features methods, devices, and kits for the isolation of analytes (e.g., a cell). A sample containing a desired analyte is introduced into a microfluidic device containing moieties that bind the desired analyte. A shear stress is applied that is great enough to prevent binding of undesired analytes and low enough to allow binding of the analyte of interest. Once bound, the desired analytes can be analyzed (e.g., counted). The invention also features methods for determining a shear stress for isolating a desired analyte.

    摘要翻译: 本发明的特征在于用于分离分析物(例如,细胞)的方法,装置和试剂盒。 将含有所需分析物的样品引入含有结合所需分析物的部分的微流体装置中。 施加的剪切应力足够大以防止不需要的分析物的结合,并且足够低以允许感兴趣的分析物的结合。 一旦结合,可以分析所需的分析物(例如,计数)。 本发明还具有确定用于分离所需分析物的剪切应力的方法。