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公开(公告)号：US08947510B2
公开(公告)日：2015-02-03
申请号：US12999515
申请日：2009-06-08
申请人： Michael G. Meyer , J. Richard Rahn , Anna V. Tourovskaia , Julia Oi Yan Yu , Christy A Lancaster , Thomas Neumann , Mark E. Fauver
发明人： Michael G. Meyer , J. Richard Rahn , Anna V. Tourovskaia , Julia Oi Yan Yu , Christy A Lancaster , Thomas Neumann , Mark E. Fauver
CPC分类号： G06T11/006 , G01N15/1434 , G01N15/1475 , G01N21/49 , G01N21/6458 , G01N2015/1445
摘要： A method for 3D imaging of a biologic object (1) in an optical tomography system where a subcellular structure of a biological object (1) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object (1) is moved relatively to a microscope objective (62) to present varying angles of view and the labeled biological object (1) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object (1) and the microscope objective (62) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object (1) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object (1).
摘要翻译： 一种用于在其中通过引入至少一种纳米颗粒 - 生物标志物标记生物体（1）的亚细胞结构的光学层析成像系统中的生物物体（1）的3D成像的方法。 标记的生物体（1）相对于显微镜物镜（62）移动以呈现不同的视角，并且用波长在150nm和900nm之间的辐射照射标记的生物物体（1）。 用彩色照相机或一组至少四个单色照相机检测在至少一个波长带内通过标记的生物物体（1）和显微镜物镜（62）发射的辐射。 形成并重构来自感测辐射的生物体（1）的多个横截面图像，以便制成标记的生物体（1）的3D图像。
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公开(公告)号：US20120105600A1
公开(公告)日：2012-05-03
申请号：US12999515
申请日：2009-06-08
申请人： Michael G. Meyer , J. Richard Rahn , Anna V. Tourovskaia , Julia Oi Yan Yu , Christy A. Lancaster , Thomas Neumann , Mark E. Fauver
发明人： Michael G. Meyer , J. Richard Rahn , Anna V. Tourovskaia , Julia Oi Yan Yu , Christy A. Lancaster , Thomas Neumann , Mark E. Fauver
IPC分类号： H04N13/02
CPC分类号： G06T11/006 , G01N15/1434 , G01N15/1475 , G01N21/49 , G01N21/6458 , G01N2015/1445
摘要： A method for 3D imaging of a biologic object (1) in an optical tomography system where a subcellular structure of a biological object (1) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object (1) is moved relatively to a microscope objective (62) to present varying angles of view and the labeled biological object (1) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object (1) and the microscope objective (62) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object (1) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object (1).
摘要翻译： 一种用于在其中通过引入至少一种纳米颗粒 - 生物标志物标记生物体（1）的亚细胞结构的光学层析成像系统中的生物物体（1）的3D成像的方法。 标记的生物体（1）相对于显微镜物镜（62）移动以呈现不同的视角，并且用波长在150nm和900nm之间的辐射照射标记的生物物体（1）。 用彩色照相机或一组至少四个单色照相机检测在至少一个波长带内通过标记的生物物体（1）和显微镜物镜（62）发射的辐射。 形成并重构来自感测辐射的生物体（1）的多个横截面图像，以便制成标记的生物体（1）的3D图像。
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公开(公告)号：US20100279268A1
公开(公告)日：2010-11-04
申请号：US12679429
申请日：2008-09-24
CPC分类号： A61L27/3808 , A61L27/3886 , C12M21/08 , C12M25/14 , C12M29/10 , C12N5/0068 , C12N5/0691 , C12N2533/50
摘要： A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seeded 1300 into a channel in a matrix at to activate competency 1304 of the cells for sprouting as microvessels based on the seeding density. The matrix channel is perfused with medium to allow parent vessels to form and for viability 1324. The parent vessels and matrix are incubated and perfused to provide for sprouting of microvessels from parent vessels into the surrounding matrix 1328. The sprouting parent vessels are grown until network forms 1332.
摘要翻译： 一种在体外创建可灌注微血管网络的方法。 将包括能够发芽的细胞类型的细胞接种在基质中的通道中，以激活基于种子密度的作为微血管发芽的细胞的能力1304。 基质通道用培养基灌注以允许母体血管形成和存活力1324.将母体血管和基质温育并灌注以提供将微血管从亲本血管发芽到周围基质1328中。将发芽的母血管生长直至网络 表格1332。
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公开(公告)号：US08445280B2
公开(公告)日：2013-05-21
申请号：US12679429
申请日：2008-09-24
IPC分类号： C12N5/07
CPC分类号： A61L27/3808 , A61L27/3886 , C12M21/08 , C12M25/14 , C12M29/10 , C12N5/0068 , C12N5/0691 , C12N2533/50
摘要： A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seeded 1300 into a channel in a matrix at to activate competency 1304 of the cells for sprouting as microvessels based on the seeding density. The matrix channel is perfused with medium to allow parent vessels to form and for viability 1324. The parent vessels and matrix are incubated and perfused to provide for sprouting of microvessels from parent vessels into the surrounding matrix 1328. The sprouting parent vessels are grown until network forms 1332.
摘要翻译： 一种在体外创建可灌注微血管网络的方法。 将包括能够发芽的细胞类型的细胞接种在基质中的通道中，以基于接种密度激活细胞的能力1304作为微血管发芽。 基质通道用培养基灌注以允许母体血管形成和存活力1324.将母体血管和基质温育并灌注以提供将微血管从亲本血管发芽到周围基质1328中。将发芽的母血管生长直至网络 表格1332。
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