公开(公告)号：US08947510B2

公开(公告)日：2015-02-03

申请号：US12999515

申请日：2009-06-08

申请人： Michael G. Meyer ,  J. Richard Rahn ,  Anna V. Tourovskaia ,  Julia Oi Yan Yu ,  Christy A Lancaster ,  Thomas Neumann ,  Mark E. Fauver

发明人： Michael G. Meyer ,  J. Richard Rahn ,  Anna V. Tourovskaia ,  Julia Oi Yan Yu ,  Christy A Lancaster ,  Thomas Neumann ,  Mark E. Fauver

IPC分类号： H04N13/02 ,  G06T11/00 ,  G01N15/14

CPC分类号： G06T11/006 ,  G01N15/1434 ,  G01N15/1475 ,  G01N21/49 ,  G01N21/6458 ,  G01N2015/1445

摘要： A method for 3D imaging of a biologic object (1) in an optical tomography system where a subcellular structure of a biological object (1) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object (1) is moved relatively to a microscope objective (62) to present varying angles of view and the labeled biological object (1) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object (1) and the microscope objective (62) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object (1) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object (1).

摘要翻译： 一种用于在其中通过引入至少一种纳米颗粒 - 生物标志物标记生物体（1）的亚细胞结构的光学层析成像系统中的生物物体（1）的3D成像的方法。 标记的生物体（1）相对于显微镜物镜（62）移动以呈现不同的视角，并且用波长在150nm和900nm之间的辐射照射标记的生物物体（1）。 用彩色照相机或一组至少四个单色照相机检测在至少一个波长带内通过标记的生物物体（1）和显微镜物镜（62）发射的辐射。 形成并重构来自感测辐射的生物体（1）的多个横截面图像，以便制成标记的生物体（1）的3D图像。

公开(公告)号：US20120105600A1

公开(公告)日：2012-05-03

申请号：US12999515

申请日：2009-06-08

申请人： Michael G. Meyer ,  J. Richard Rahn ,  Anna V. Tourovskaia ,  Julia Oi Yan Yu ,  Christy A. Lancaster ,  Thomas Neumann ,  Mark E. Fauver

发明人： Michael G. Meyer ,  J. Richard Rahn ,  Anna V. Tourovskaia ,  Julia Oi Yan Yu ,  Christy A. Lancaster ,  Thomas Neumann ,  Mark E. Fauver

IPC分类号： H04N13/02

CPC分类号： G06T11/006 ,  G01N15/1434 ,  G01N15/1475 ,  G01N21/49 ,  G01N21/6458 ,  G01N2015/1445

摘要： A method for 3D imaging of a biologic object (1) in an optical tomography system where a subcellular structure of a biological object (1) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object (1) is moved relatively to a microscope objective (62) to present varying angles of view and the labeled biological object (1) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object (1) and the microscope objective (62) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object (1) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object (1).

摘要翻译： 一种用于在其中通过引入至少一种纳米颗粒 - 生物标志物标记生物体（1）的亚细胞结构的光学层析成像系统中的生物物体（1）的3D成像的方法。 标记的生物体（1）相对于显微镜物镜（62）移动以呈现不同的视角，并且用波长在150nm和900nm之间的辐射照射标记的生物物体（1）。 用彩色照相机或一组至少四个单色照相机检测在至少一个波长带内通过标记的生物物体（1）和显微镜物镜（62）发射的辐射。 形成并重构来自感测辐射的生物体（1）的多个横截面图像，以便制成标记的生物体（1）的3D图像。

公开(公告)号：US20100279268A1

公开(公告)日：2010-11-04

申请号：US12679429

申请日：2008-09-24

申请人： Thomas Neumann ,  Anna V. Tourovskaia ,  Mark E. Fauver ,  Julia Oi Yan Yu

发明人： Thomas Neumann ,  Anna V. Tourovskaia ,  Mark E. Fauver ,  Julia Oi Yan Yu

IPC分类号： C12N5/02 ,  C12M1/00

CPC分类号： A61L27/3808 ,  A61L27/3886 ,  C12M21/08 ,  C12M25/14 ,  C12M29/10 ,  C12N5/0068 ,  C12N5/0691 ,  C12N2533/50

摘要： A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seeded 1300 into a channel in a matrix at to activate competency 1304 of the cells for sprouting as microvessels based on the seeding density. The matrix channel is perfused with medium to allow parent vessels to form and for viability 1324. The parent vessels and matrix are incubated and perfused to provide for sprouting of microvessels from parent vessels into the surrounding matrix 1328. The sprouting parent vessels are grown until network forms 1332.

摘要翻译： 一种在体外创建可灌注微血管网络的方法。 将包括能够发芽的细胞类型的细胞接种在基质中的通道中，以激活基于种子密度的作为微血管发芽的细胞的能力1304。 基质通道用培养基灌注以允许母体血管形成和存活力1324.将母体血管和基质温育并灌注以提供将微血管从亲本血管发芽到周围基质1328中。将发芽的母血管生长直至网络 表格1332。

公开(公告)号：US07907765B2

公开(公告)日：2011-03-15

申请号：US11532648

申请日：2006-09-18

申请人： Mark E. Fauver ,  Eric J. Seibel ,  Michael G. Meyer ,  Alan C. Nelson ,  J. Richard Rahn ,  Thomas Neumann ,  Roger H. Johnson

发明人： Mark E. Fauver ,  Eric J. Seibel ,  Michael G. Meyer ,  Alan C. Nelson ,  J. Richard Rahn ,  Thomas Neumann ,  Roger H. Johnson

IPC分类号： G06K9/00

CPC分类号： G01N21/4795 ,  G01N15/147 ,  G01N15/1475

摘要： An optical tomography system for imaging an object of interest including a light source for illuminating the object of interest with a plurality of radiation beams. The object of interest is held within an object containing tube such that it is illuminated by the plurality of radiation beams to produce emerging radiation from the object containing tube, a detector array is located to receive the emerging radiation and produce imaging data used by a mechanism for tracking the object of interest.

摘要翻译： 一种光学断层摄影系统，用于对感兴趣的物体成像，包括用多个辐射束照射感兴趣物体的光源。 感兴趣的物体被保持在物体容纳管内，使得其被多个辐射束照射以产生来自物体容纳管的出射辐射，检测器阵列被定位成接收新出现的辐射并产生由机构使用的成像数据 用于跟踪感兴趣的对象。

公开(公告)号：US20100322494A1

公开(公告)日：2010-12-23

申请号：US11532648

申请日：2006-09-18

申请人： Mark E. Fauver ,  Eric J. Seibel ,  Michael G. Meyer ,  Alan C. Nelson ,  J. Richard Rahn ,  Thomas Neumann ,  Roger H. Johnson

发明人： Mark E. Fauver ,  Eric J. Seibel ,  Michael G. Meyer ,  Alan C. Nelson ,  J. Richard Rahn ,  Thomas Neumann ,  Roger H. Johnson

IPC分类号： G06K9/00

CPC分类号： G01N21/4795 ,  G01N15/147 ,  G01N15/1475

摘要： An optical tomography system for imaging an object of interest including a light source for illuminating the object of interest with a plurality of radiation beams. The object of interest is held within an object containing tube such that it is illuminated by the plurality of radiation beams to produce emerging radiation from the object containing tube, a detector array is located to receive the emerging radiation and produce imaging data used by a mechanism for tracking the object of interest.

摘要翻译： 一种光学断层摄影系统，用于对感兴趣的物体成像，包括用多个辐射束照射感兴趣物体的光源。 感兴趣的物体被保持在物体容纳管内，使得其被多个辐射束照射以产生来自物体容纳管的出射辐射，检测器阵列被定位成接收新出现的辐射并产生由机构使用的成像数据 用于跟踪感兴趣的对象。

公开(公告)号：US08445280B2

公开(公告)日：2013-05-21

申请号：US12679429

申请日：2008-09-24

申请人： Thomas Neumann ,  Anna Tourovskaia ,  Mark E. Fauver ,  Julia Oi Yan Yu

发明人： Thomas Neumann ,  Anna Tourovskaia ,  Mark E. Fauver ,  Julia Oi Yan Yu

IPC分类号： C12N5/07

CPC分类号： A61L27/3808 ,  A61L27/3886 ,  C12M21/08 ,  C12M25/14 ,  C12M29/10 ,  C12N5/0068 ,  C12N5/0691 ,  C12N2533/50

摘要： A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seeded 1300 into a channel in a matrix at to activate competency 1304 of the cells for sprouting as microvessels based on the seeding density. The matrix channel is perfused with medium to allow parent vessels to form and for viability 1324. The parent vessels and matrix are incubated and perfused to provide for sprouting of microvessels from parent vessels into the surrounding matrix 1328. The sprouting parent vessels are grown until network forms 1332.

摘要翻译： 一种在体外创建可灌注微血管网络的方法。 将包括能够发芽的细胞类型的细胞接种在基质中的通道中，以基于接种密度激活细胞的能力1304作为微血管发芽。 基质通道用培养基灌注以允许母体血管形成和存活力1324.将母体血管和基质温育并灌注以提供将微血管从亲本血管发芽到周围基质1328中。将发芽的母血管生长直至网络 表格1332。

公开(公告)号：US20080285827A1

公开(公告)日：2008-11-20

申请号：US11750924

申请日：2007-05-18

申请人： Michael G. Meyer ,  J. Richard Rahn ,  Mark E. Fauver

发明人： Michael G. Meyer ,  J. Richard Rahn ,  Mark E. Fauver

IPC分类号： G06K9/00

CPC分类号： G06T11/005

摘要： A method for reconstructing three-dimensional (3D) tomographic images. A set of pseudo-projection images of an object is acquired. Error corrections are applied to the set of pseudo-projection images to produce a set of corrected pseudo-projection images. The set of corrected pseudo-projection images are processed to produce (3D) tomographic images.

摘要翻译： 一种重建三维（3D）断层图像的方法。 获取对象的一组伪投影图像。 误差校正被应用于伪投影图像集合以产生一组校正的伪投影图像。 处理该组校正的伪投影图像以产生（3D）断层图像。

公开(公告)号：US07835561B2

公开(公告)日：2010-11-16

申请号：US11750924

申请日：2007-05-18

申请人： Michael G. Meyer ,  J. Richard Rahn ,  Mark E. Fauver

发明人： Michael G. Meyer ,  J. Richard Rahn ,  Mark E. Fauver

IPC分类号： G06K9/00 ,  G01N21/00

CPC分类号： G06T11/005

摘要： A method for reconstructing three-dimensional (3D) tomographic images. A set of pseudo-projection images of an object is acquired. Error corrections are applied to the set of pseudo-projection images to produce a set of corrected pseudo-projection images. The set of corrected pseudo-projection images are processed to produce (3D) tomographic images.

摘要翻译： 一种重建三维（3D）断层图像的方法。 获取对象的一组伪投影图像。 误差校正被应用于伪投影图像集合以产生一组校正的伪投影图像。 处理该组校正的伪投影图像以产生（3D）断层图像。

公开(公告)号：US20120145926A1

公开(公告)日：2012-06-14

申请号：US13402149

申请日：2012-02-22

申请人： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

发明人： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

IPC分类号： G01N21/64

CPC分类号： G01N21/4795 ,  G01N15/1436 ,  G01N2015/1445 ,  G01N2021/1787 ,  G06T11/006

摘要： A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.

摘要翻译： 用于光学断层摄影系统中的细胞的3D成像的方法包括相对于显微镜物镜移动生物体以呈现不同的视角。 用具有限制在150nm和390nm之间的波长的光谱带宽的辐射照射该生物物体。 利用摄像机从多个不同的视角检测透过生物物体和显微镜物镜的辐射。 形成来自感测辐射的生物物体的多个伪投影，并重建多个伪投影以形成该细胞的3D图像。

公开(公告)号：US07811825B2

公开(公告)日：2010-10-12

申请号：US10964508

申请日：2004-10-13

申请人： Mark E. Fauver ,  J. Richard Rahn ,  Eric J. Seibel ,  Alan C. Nelson

发明人： Mark E. Fauver ,  J. Richard Rahn ,  Eric J. Seibel ,  Alan C. Nelson

IPC分类号： G01N33/48

CPC分类号： G02B21/002 ,  G02B21/365

摘要： A scanning method for scanning samples of biological cells using optical tomography includes preparing, acquiring, reconstructing and viewing three-dimensional images of cell samples. Concentration and enrichment of the cell sample follows. The cell sample is stained. Cells are isolated from the cell sample and purified. A cell/solvent mixture is injected into a gel by centrifugation. A cell/gel mixture is injected into a capillary tube until a cell appears centered in a field of view using a stopped-flow method. An optical imaging system, such as a fixed or variable motion optical tomography system acquires a projection image. The sample is rotated about a tube axis to generate additional projections. Once image acquisition is completed, the acquired image projections are corrected for errors. A computer or other equivalent processor is used to compute filtered backprojection information for 3D reconstruction.

摘要翻译： 使用光学断层摄影扫描生物细胞样本的扫描方法包括准备，获取，重建和观察细胞样品的三维图像。 细胞样品的浓缩和浓缩如下。 细胞样品染色。 细胞从细胞样品中分离并纯化。 通过离心将细胞/溶剂混合物注入凝胶。 将细胞/凝胶混合物注入毛细管中，直到细胞以视野停止流出中心为止。 诸如固定或可变运动光学断层摄影系统的光学成像系统获取投影图像。 样品围绕管轴旋转以产生额外的突起。 一旦图像获取完成，就获得的图像投影被校正以用于错误。 计算机或其他等效处理器用于计算用于3D重建的滤波反投影信息。