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公开(公告)号：US08947510B2
公开(公告)日：2015-02-03
申请号：US12999515
申请日：2009-06-08
申请人： Michael G. Meyer , J. Richard Rahn , Anna V. Tourovskaia , Julia Oi Yan Yu , Christy A Lancaster , Thomas Neumann , Mark E. Fauver
发明人： Michael G. Meyer , J. Richard Rahn , Anna V. Tourovskaia , Julia Oi Yan Yu , Christy A Lancaster , Thomas Neumann , Mark E. Fauver
CPC分类号： G06T11/006 , G01N15/1434 , G01N15/1475 , G01N21/49 , G01N21/6458 , G01N2015/1445
摘要： A method for 3D imaging of a biologic object (1) in an optical tomography system where a subcellular structure of a biological object (1) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object (1) is moved relatively to a microscope objective (62) to present varying angles of view and the labeled biological object (1) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object (1) and the microscope objective (62) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object (1) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object (1).
摘要翻译： 一种用于在其中通过引入至少一种纳米颗粒 - 生物标志物标记生物体（1）的亚细胞结构的光学层析成像系统中的生物物体（1）的3D成像的方法。 标记的生物体（1）相对于显微镜物镜（62）移动以呈现不同的视角，并且用波长在150nm和900nm之间的辐射照射标记的生物物体（1）。 用彩色照相机或一组至少四个单色照相机检测在至少一个波长带内通过标记的生物物体（1）和显微镜物镜（62）发射的辐射。 形成并重构来自感测辐射的生物体（1）的多个横截面图像，以便制成标记的生物体（1）的3D图像。
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公开(公告)号：US20120105600A1
公开(公告)日：2012-05-03
申请号：US12999515
申请日：2009-06-08
申请人： Michael G. Meyer , J. Richard Rahn , Anna V. Tourovskaia , Julia Oi Yan Yu , Christy A. Lancaster , Thomas Neumann , Mark E. Fauver
发明人： Michael G. Meyer , J. Richard Rahn , Anna V. Tourovskaia , Julia Oi Yan Yu , Christy A. Lancaster , Thomas Neumann , Mark E. Fauver
IPC分类号： H04N13/02
CPC分类号： G06T11/006 , G01N15/1434 , G01N15/1475 , G01N21/49 , G01N21/6458 , G01N2015/1445
摘要： A method for 3D imaging of a biologic object (1) in an optical tomography system where a subcellular structure of a biological object (1) is labeled by introducing at least one nanoparticle-biomarker. The labeled biological object (1) is moved relatively to a microscope objective (62) to present varying angles of view and the labeled biological object (1) is illuminated with radiation having wavelengths between 150 nm and 900 nm. Radiation transmitted through the labeled biological object (1) and the microscope objective (62) within at least one wavelength bands is sensed with a color camera, or with a set of at least four monochrome cameras. A plurality of cross-sectional images of the biological object (1) from the sensed radiation is formed and reconstructed to make a 3D image of the labeled biological object (1).
摘要翻译： 一种用于在其中通过引入至少一种纳米颗粒 - 生物标志物标记生物体（1）的亚细胞结构的光学层析成像系统中的生物物体（1）的3D成像的方法。 标记的生物体（1）相对于显微镜物镜（62）移动以呈现不同的视角，并且用波长在150nm和900nm之间的辐射照射标记的生物物体（1）。 用彩色照相机或一组至少四个单色照相机检测在至少一个波长带内通过标记的生物物体（1）和显微镜物镜（62）发射的辐射。 形成并重构来自感测辐射的生物体（1）的多个横截面图像，以便制成标记的生物体（1）的3D图像。
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公开(公告)号：US20100279268A1
公开(公告)日：2010-11-04
申请号：US12679429
申请日：2008-09-24
CPC分类号： A61L27/3808 , A61L27/3886 , C12M21/08 , C12M25/14 , C12M29/10 , C12N5/0068 , C12N5/0691 , C12N2533/50
摘要： A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seeded 1300 into a channel in a matrix at to activate competency 1304 of the cells for sprouting as microvessels based on the seeding density. The matrix channel is perfused with medium to allow parent vessels to form and for viability 1324. The parent vessels and matrix are incubated and perfused to provide for sprouting of microvessels from parent vessels into the surrounding matrix 1328. The sprouting parent vessels are grown until network forms 1332.
摘要翻译： 一种在体外创建可灌注微血管网络的方法。 将包括能够发芽的细胞类型的细胞接种在基质中的通道中，以激活基于种子密度的作为微血管发芽的细胞的能力1304。 基质通道用培养基灌注以允许母体血管形成和存活力1324.将母体血管和基质温育并灌注以提供将微血管从亲本血管发芽到周围基质1328中。将发芽的母血管生长直至网络 表格1332。
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公开(公告)号：US07907765B2
公开(公告)日：2011-03-15
申请号：US11532648
申请日：2006-09-18
申请人： Mark E. Fauver , Eric J. Seibel , Michael G. Meyer , Alan C. Nelson , J. Richard Rahn , Thomas Neumann , Roger H. Johnson
发明人： Mark E. Fauver , Eric J. Seibel , Michael G. Meyer , Alan C. Nelson , J. Richard Rahn , Thomas Neumann , Roger H. Johnson
IPC分类号： G06K9/00
CPC分类号： G01N21/4795 , G01N15/147 , G01N15/1475
摘要： An optical tomography system for imaging an object of interest including a light source for illuminating the object of interest with a plurality of radiation beams. The object of interest is held within an object containing tube such that it is illuminated by the plurality of radiation beams to produce emerging radiation from the object containing tube, a detector array is located to receive the emerging radiation and produce imaging data used by a mechanism for tracking the object of interest.
摘要翻译： 一种光学断层摄影系统，用于对感兴趣的物体成像，包括用多个辐射束照射感兴趣物体的光源。 感兴趣的物体被保持在物体容纳管内，使得其被多个辐射束照射以产生来自物体容纳管的出射辐射，检测器阵列被定位成接收新出现的辐射并产生由机构使用的成像数据 用于跟踪感兴趣的对象。
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公开(公告)号：US20100322494A1
公开(公告)日：2010-12-23
申请号：US11532648
申请日：2006-09-18
申请人： Mark E. Fauver , Eric J. Seibel , Michael G. Meyer , Alan C. Nelson , J. Richard Rahn , Thomas Neumann , Roger H. Johnson
发明人： Mark E. Fauver , Eric J. Seibel , Michael G. Meyer , Alan C. Nelson , J. Richard Rahn , Thomas Neumann , Roger H. Johnson
IPC分类号： G06K9/00
CPC分类号： G01N21/4795 , G01N15/147 , G01N15/1475
摘要： An optical tomography system for imaging an object of interest including a light source for illuminating the object of interest with a plurality of radiation beams. The object of interest is held within an object containing tube such that it is illuminated by the plurality of radiation beams to produce emerging radiation from the object containing tube, a detector array is located to receive the emerging radiation and produce imaging data used by a mechanism for tracking the object of interest.
摘要翻译： 一种光学断层摄影系统，用于对感兴趣的物体成像，包括用多个辐射束照射感兴趣物体的光源。 感兴趣的物体被保持在物体容纳管内，使得其被多个辐射束照射以产生来自物体容纳管的出射辐射，检测器阵列被定位成接收新出现的辐射并产生由机构使用的成像数据 用于跟踪感兴趣的对象。
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公开(公告)号：US08445280B2
公开(公告)日：2013-05-21
申请号：US12679429
申请日：2008-09-24
IPC分类号： C12N5/07
CPC分类号： A61L27/3808 , A61L27/3886 , C12M21/08 , C12M25/14 , C12M29/10 , C12N5/0068 , C12N5/0691 , C12N2533/50
摘要： A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seeded 1300 into a channel in a matrix at to activate competency 1304 of the cells for sprouting as microvessels based on the seeding density. The matrix channel is perfused with medium to allow parent vessels to form and for viability 1324. The parent vessels and matrix are incubated and perfused to provide for sprouting of microvessels from parent vessels into the surrounding matrix 1328. The sprouting parent vessels are grown until network forms 1332.
摘要翻译： 一种在体外创建可灌注微血管网络的方法。 将包括能够发芽的细胞类型的细胞接种在基质中的通道中，以基于接种密度激活细胞的能力1304作为微血管发芽。 基质通道用培养基灌注以允许母体血管形成和存活力1324.将母体血管和基质温育并灌注以提供将微血管从亲本血管发芽到周围基质1328中。将发芽的母血管生长直至网络 表格1332。
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公开(公告)号：US08003388B2
公开(公告)日：2011-08-23
申请号：US11860471
申请日：2007-09-24
申请人： Thomas Neumann
发明人： Thomas Neumann
IPC分类号： C12N5/00
CPC分类号： A61L27/3808 , C12M21/08 , C12M23/06 , C12M23/20 , C12M25/14 , C12M29/10 , C12N5/0068 , C12N5/0691 , C12N2533/50
摘要： A method for creating networks of perfusable microvessels in vitro. A mandrel is drawn through a matrix to form a channel through the matrix. Cells are injected into the channel. The matrix is incubated to allow the cells to attach inside the channel. The channel is perfused to remove unattached cells to create a parent vessel, where the parent vessel includes a perfusable hollow channel lined with cells in the matrix. The parent vessel is induced to create sprouts into the surrounding matrix gel so as to form a microvessel network. The microvessel network is subjected to luminal perfusion through the parent vessel.
摘要翻译： 一种在体外创建可灌注微血管网络的方法。 心轴通过矩阵绘制以形成通过矩阵的通道。 细胞注入通道。 将基质温育以使细胞附着在通道内。 灌注通道以除去未附着的细胞以产生亲本血管，其中母体血管包括排列在基质中的细胞的可灌注中空通道。 诱导母体容器以产生芽至周围的基质凝胶中，从而形成微血管网络。 微血管网络通过母体血管进行腔灌注。
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公开(公告)号：US20050095661A1
公开(公告)日：2005-05-05
申请号：US10479843
申请日：2002-06-07
CPC分类号： G01N33/6848 , C07K1/12 , G01N33/6821
摘要： Provided is a method for characterising a polypeptide, which method comprises the steps of; (a) optionnally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more ε-amino groups that are present with a lysine reactive agent; (e) analysing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint.
摘要翻译： 提供一种表征多肽的方法，该方法包括以下步骤： （a）选择性地减少多肽中的半胱氨酸二硫桥以形成游离的硫醇，并且封端游离的硫醇; （b）用序列特异性切割试剂切割多肽以形成肽片段; （c）任选地使切割试剂失活; （d）封端与赖氨酸反应剂一起存在的一个或多个ε-氨基; （e）通过质谱分析肽片段以形成多肽的质量指纹图谱; 和（f）从质量指纹图谱确定多肽的身份。
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公开(公告)号：US20050048489A1
公开(公告)日：2005-03-03
申请号：US10489341
申请日：2002-09-16
申请人： Andrew Thompson , Christian Hamon , Jurgen Schafer , Karsten Kuhn , Joseph Schwarz , Thomas Neumann
发明人： Andrew Thompson , Christian Hamon , Jurgen Schafer , Karsten Kuhn , Joseph Schwarz , Thomas Neumann
IPC分类号： G01N27/62 , B01J20/281 , G01N30/04 , G01N30/72 , G01N30/88 , G01N33/48 , G01N33/483 , G01N33/50 , G01N33/53 , G01N33/58 , G01N33/68 , H01J49/04 , C12Q1/68 , C07H21/04
CPC分类号： G01N33/6848 , G01N33/68 , G01N33/6851 , G01N2458/15 , H01J49/04
摘要： Provided is a set of two or more mass labels, each label in the set comprising a mass marker moiety attached via a cleavable linker having at least one amide bond to a mass normalisation moiety, wherein the aggregate mass of each label in the set may be the same or different and the mass of the mass marker moiety of each label in the set may be the same or different, and wherein in any group of labels within the set having a mass marker moiety of a common mass each label has an aggregate mass different from all other labels in that group, and wherein in any group of labels within the set having a common aggregate mass each label has a mass marker moiety having a mass different from that of all other mass marker moieties in that group, such that all of the mass labels in the set are distinguishable from each other by mass spectromety, and wherein the mass marker moiety comprises an amino acid and the mass normalisation moiety comprises an amino acid.
摘要翻译： 提供了一组两个或更多个质量标签，该组中的每个标签包含通过具有至少一个酰胺键与质量标准化部分连接的质量标记部分，其中该组中每个标签的总质量可以是 相同或不同，组中每个标记物的质量标记物部分的质量可以相同或不同，并且其中在具有共同质量的质量标记物部分的组中的任何标签组中，每个标签具有总体质量 不同于该组中的所有其它标记，并且其中在组中具有共同聚集体的任何标签组中，每个标记具有与该组中所有其他质量标记部分的质量不同的质量标记部分，使得全部 组中的质量标记物通过质谱可以彼此区分，并且其中质量标记物部分包含氨基酸，质量标准化部分包含氨基酸。
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公开(公告)号：US08846307B2
公开(公告)日：2014-09-30
申请号：US12541707
申请日：2009-08-14
申请人： Thomas Neumann
发明人： Thomas Neumann
CPC分类号： C12N5/0691 , C12M21/08 , C12M23/06 , C12M23/16 , C12M23/20 , C12M29/10 , C12N2533/50
摘要： Creating a tissue structure in vitro includes juxtaposing mandrels on a culture/perfusion device frame where the mandrels are spaced apart substantially parallel to each other and connecting the mandrels to tubes including an upstream tubes and downstream tubes. The upstream tubes are connected with an upstream manifold and the downstream tubes are connected to a downstream manifold. The frame and the mandrels are sterilized, coated and seeded with cells that multiply and form circular layers around each of the mandrels until the circular layers merge into a tissue structure which is subjected to a growth medium. The mandrels are extracted and the tissue structure is perfused.
摘要翻译： 在体外创建组织结构包括在培养/灌注装置框架上并置心轴，其中心轴彼此间隔开并且将心轴连接到包括上游管和下游管的管。 上游管与上游歧管连接，下游管连接到下游歧管。 框架和心轴被消毒，涂覆并种植有细胞，每个细胞在每个心轴周围形成圆形层，直到圆形层合并成经历生长培养基的组织结构。 提取心轴并灌注组织结构。
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