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公开(公告)号：US20100323405A1
公开(公告)日：2010-12-23
申请号：US12665788
申请日：2008-06-23
IPC分类号： C12P19/34
CPC分类号： C12Q1/6846 , C12Q2527/15 , C12Q2527/101
摘要： A method of amplifying a nucleic acid, the method comprising cycling an amplification-ready droplet through a temperature gradient to locations within the temperature gradient suitable for effecting steps in an amplification reaction.
摘要翻译： 一种扩增核酸的方法，所述方法包括使扩增准备的液滴通过温度梯度循环到适于在扩增反应中进行步骤的温度梯度内的位置。
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公开(公告)号：US08951732B2
公开(公告)日：2015-02-10
申请号：US12665788
申请日：2008-06-23
CPC分类号： C12Q1/6846 , C12Q2527/15 , C12Q2527/101
摘要： A method of amplifying a nucleic acid, the method comprising cycling an amplification-ready droplet through a temperature gradient to locations within the temperature gradient suitable for effecting steps in an amplification reaction.
摘要翻译： 一种扩增核酸的方法，所述方法包括使扩增准备的液滴通过温度梯度循环到适于在扩增反应中进行步骤的温度梯度内的位置。
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公开(公告)号：US20130233712A1
公开(公告)日：2013-09-12
申请号：US13870709
申请日：2013-04-25
IPC分类号： B01L3/02
CPC分类号： B01L3/502792 , B01L3/0241 , B01L3/50273 , B01L2200/06 , B01L2300/06 , B01L2400/0427 , B41J2/14
摘要： A method of manipulating a droplet comprising providing a substrate comprising a surface; an elongated transport electrode disposed on the substrate surface, the elongated transport electrode having a first and a second end and configured to impart a gradient force to the droplet; and one or more wires for providing power to the transport electrode; and providing power to the one or more wires to effect the gradient force and thereby transport the droplet along the length of the elongated transport electrode from the first end to the second end.
摘要翻译： 一种操纵液滴的方法，包括提供包括表面的基底; 设置在基板表面上的细长输送电极，细长输送电极具有第一和第二端并且被配置为向液滴施加梯度力; 以及用于向输送电极供电的一根或多根线; 并且向所述一个或多个电线提供电力以实现所述梯度力，从而将所述液滴沿着所述细长输送电极的长度从所述第一端输送到所述第二端。
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4.发明申请Droplet Actuator Configurations and Methods of Conducting Droplet Operations 审中-公开液滴执行器配置和执行液滴操作的方法公开(公告)号：US20100270156A1
公开(公告)日：2010-10-28
申请号：US12682830
申请日：2008-12-23
申请人： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zhishan Hua , Arjun Sudarsan , Philip Paik
发明人： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zhishan Hua , Arjun Sudarsan , Philip Paik
IPC分类号： C07K1/26
CPC分类号： B01L3/52 , B01F13/0071 , B01F13/0076 , B01L3/0241 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/143 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/0427 , B01L2400/06 , B01L2400/0688 , F04B19/006
摘要： A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration comprises one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.
摘要翻译： 具有与液滴操作表面相关联的液滴形成电极配置的液滴致动器，其中所述电极配置包括一个或多个电极，其被配置为在液滴操作表面上形成子液滴期间控制液滴的体积。 还提供了制造和使用液滴致动器的方法。
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公开(公告)号：US08460528B2
公开(公告)日：2013-06-11
申请号：US12681879
申请日：2008-10-14
IPC分类号： G01N27/26
CPC分类号： B01L3/5027 , B01J2219/00371 , B01J2219/00378 , B01J2219/00385 , B01J2219/00515 , B01J2219/00518 , B01J2219/0065 , B01J2219/00653 , B01L3/502715 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/0668 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/0845 , B01L2300/089 , B01L2400/0427 , B01L2400/0478 , B01L2400/0487
摘要： On-board reagent storage, handling, and dispensing apparatuses and methods for droplet actuator systems are provided. In one embodiment, a fluidic analyzer is provided and includes a droplet actuator including a substrate including a plurality of electrodes configured to actuate a droplet and a reagent storage component in selective fluid communication with the droplet actuator and including a reagent configured to combine with a solvent at a time of use. In another embodiment, a method of conducting fluidic analysis is provided and includes storing a reagent within a reagent storage component in fluid communication with a droplet actuator including a plurality of electrodes configured to actuate a droplet and combining the reagent with a solvent at a time for use within the droplet actuator.
摘要翻译： 提供了用于液滴致动器系统的机载试剂储存，处理和分配装置和方法。 在一个实施例中，提供了一种流体分析器，其包括液滴致动器，该液滴致动器包括基底，该基底包括配置成致动液滴的多个电极和与液滴致动器选择性流体连通的试剂储存部件，并且包括配置成与溶剂组合的试剂 在使用时。 在另一个实施例中，提供了一种进行流体分析的方法，包括将试剂储存在与液滴致动器流体连通的液体致动器内，所述液滴致动器包括配置成致动液滴的多个电极并且一次将溶剂与溶剂组合 在液滴致动器内使用。
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公开(公告)号：US20100282609A1
公开(公告)日：2010-11-11
申请号：US12681879
申请日：2008-10-14
IPC分类号： B01J19/08
CPC分类号： B01L3/5027 , B01J2219/00371 , B01J2219/00378 , B01J2219/00385 , B01J2219/00515 , B01J2219/00518 , B01J2219/0065 , B01J2219/00653 , B01L3/502715 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/0668 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/0845 , B01L2300/089 , B01L2400/0427 , B01L2400/0478 , B01L2400/0487
摘要： On-board reagent storage, handling, and dispensing apparatuses and methods for droplet actuator systems are provided. In one embodiment, a fluidic analyzer is provided and includes a droplet actuator including a substrate including a plurality of electrodes configured to actuate a droplet and a reagent storage component in selective fluid communication with the droplet actuator and including a reagent configured to combine with a solvent at a time of use. In another embodiment, a method of conducting fluidic analysis is provided and includes storing a reagent within a reagent storage component in fluid communication with a droplet actuator including a plurality of electrodes configured to actuate a droplet and combining the reagent with a solvent at a time for use within the droplet actuator.
摘要翻译： 提供了用于液滴致动器系统的机载试剂储存，处理和分配装置和方法。 在一个实施例中，提供了一种流体分析器，其包括液滴致动器，该液滴致动器包括基底，该基底包括配置成致动液滴的多个电极和与液滴致动器选择性流体连通的试剂储存部件，并且包括配置成与溶剂组合的试剂 在使用时。 在另一个实施例中，提供了一种进行流体分析的方法，包括将试剂储存在与液滴致动器流体连通的液体致动器内，所述液滴致动器包括配置成致动液滴的多个电极并且一次将溶剂与溶剂组合 在液滴致动器内使用。
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公开(公告)号：US20100025250A1
公开(公告)日：2010-02-04
申请号：US12529041
申请日：2008-03-03
CPC分类号： B01L3/502792 , B01L3/0241 , B01L3/50273 , B01L2200/06 , B01L2300/06 , B01L2400/0427 , B41J2/14
摘要： The objective of this research is to model and design a microfluidic system that uses electrostatic fields to induce movement of discrete droplets of solution. Of particular interest is movement of droplets of H2O for use in biological testing with lab-on a-chip and μTAS systems. Using computer modeling, the electric-fields for planar electrode configurations positioned on an insulating substrate are calculated for a hemispherical drop of H2O on the substrate at various positions. From these electric-fields the force on the drop is calculated. These models show that electrostatic actuation of droplets of H2O is possible. However, as the complexity of the model increases the properties of the system become less desirable and actuation may not be possible. Using microfabrication techniques, the modeled microfluidic systems have been built for testing using a Kapton substrate with copper electrodes. Hexadecenyltrichlorosilane (HTS), a self-assembled monolayer, and its oxidant have been studied and found capable of providing hydrophobic and hydrophilic surface coatings for the systems.
摘要翻译： 本研究的目的是建立和设计一个使用静电场诱导离散液滴移动的微流体系统。 特别令人感兴趣的是使用实验室上的芯片和muTAS系统用于生物测试的H 2 O滴。 使用计算机建模，计算位于绝缘基板上的平面电极配置的电场，用于在不同位置的衬底上的半球形H 2 O的下降。 从这些电场计算出液滴上的力。 这些模型显示H2O的液滴的静电驱动是可能的。 然而，随着模型的复杂性增加，系统的性能变得不太可取，并且致动可能是不可能的。 使用微细加工技术，已经建立了用于使用具有铜电极的Kapton衬底进行测试的建模的微流体系统。 已经研究了十六碳烯三氯硅烷（HTS），一种自组装单层及其氧化剂，能够为系统提供疏水和亲水的表面涂层。
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公开(公告)号：US20100143963A1
公开(公告)日：2010-06-10
申请号：US12707081
申请日：2010-02-17
申请人： Michael G. Pollack , Philip Paik
发明人： Michael G. Pollack , Philip Paik
CPC分类号： B01L3/502792 , B01L2200/025 , B01L2200/04 , B01L2300/089 , B01L2400/0427 , G01N21/59 , G01N2021/035 , G01N2035/1046
摘要： The invention provides a droplet actuator drive. In certain embodiments, the droplet actuator drive may include a detection apparatus for sensing a property of a droplet on a droplet actuator; circuitry for controlling the detection apparatus electronically coupled to the detection apparatus; a droplet actuator cartridge connector; circuitry for controlling a droplet actuator coupled to the droplet actuator connector; and/or a means for coupling the droplet actuator circuitry to a processor. Systems, kits and methods of conducting assays are also provided.
摘要翻译： 本发明提供一种液滴致动器驱动装置。 在某些实施例中，液滴致动器驱动器可以包括用于感测液滴致动器上的液滴的特性的检测装置; 用于控制检测装置的电路，电子耦合到检测装置; 液滴致动器盒连接器; 用于控制耦合到液滴致动器连接器的液滴致动器的电路; 和/或用于将液滴致动器电路耦合到处理器的装置。 还提供了进行测定的系统，试剂盒和方法。
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公开(公告)号：US08041463B2
公开(公告)日：2011-10-18
申请号：US12707081
申请日：2010-02-17
申请人： Michael G. Pollack , Philip Paik
发明人： Michael G. Pollack , Philip Paik
CPC分类号： B01L3/502792 , B01L2200/025 , B01L2200/04 , B01L2300/089 , B01L2400/0427 , G01N21/59 , G01N2021/035 , G01N2035/1046
摘要： A droplet actuator drive including a detection apparatus for sensing a property of a droplet on a droplet actuator; circuitry for controlling the detection apparatus electronically coupled to the detection apparatus; a droplet actuator cartridge connector arranged so that when a droplet actuator cartridge electronically is coupled thereto: the droplet actuator cartridge is aligned with the detection apparatus; and the detection apparatus can sense the property of the droplet on a droplet actuator; circuitry for controlling a droplet actuator coupled to the droplet actuator connector; and the droplet actuator circuitry may be coupled to a processor.
摘要翻译： 液滴致动器驱动器，其包括用于感测液滴致动器上的液滴的特性的检测装置; 用于控制检测装置的电路，电子耦合到所述检测装置; 液滴致动器盒连接器布置成使得当液滴致动器盒电子地耦合到其上时：液滴致动器盒与检测装置对准; 并且检测装置可以感测液滴致动器上的液滴的特性; 用于控制耦合到液滴致动器连接器的液滴致动器的电路; 并且液滴致动器电路可以耦合到处理器。
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公开(公告)号：US20080105549A1
公开(公告)日：2008-05-08
申请号：US11936289
申请日：2007-11-07
申请人： Vamsee Pamela , Michael Pollack , Philip Paik , Hong Ren , Richard Fair
发明人： Vamsee Pamela , Michael Pollack , Philip Paik , Hong Ren , Richard Fair
IPC分类号： G01N27/26
CPC分类号： B01F13/0071 , B01F13/0076 , B01F13/0084 , B01J2219/00479 , B01J2219/00653 , B01L3/502792 , B01L2300/089 , B01L2300/165 , B01L2400/0415 , B01L2400/0427 , C40B60/14 , G01N15/14 , G01N2035/1046 , G02B26/005 , Y10T436/11 , Y10T436/113332 , Y10T436/117497 , Y10T436/118339 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： Methods for sampling a liquid flow are provided. The method includes supplying a continuous liquid flow from a continuous flow fluid input source to a surface along a first flow path. The method additionally includes sampling the continuous liquid flow by performing an electric field-based technique to split off a sample droplet from a portion of the liquid flow, whereby the sample droplet is distinct from the liquid flow and controllable independently of the liquid flow, wherein the electric field-based technique is performed by providing a set of electrodes on the surface and selectively biasing the set of electrodes, whereby the sample droplet is formed on one of the set of electrodes.
摘要翻译： 提供了液体取样方法。 该方法包括将连续的液体流从连续流体流体输入源提供到沿着第一流动路径的表面。 该方法另外包括通过执行基于电场的技术对液体流的一部分分离样品液滴来对连续液体流进行采样，由此样品液滴与液体流不同，并且可独立于液体流动而控制，其中 通过在表面上设置一组电极并选择性地偏置该组电极来执行基于电场的技术，由此在一组电极中形成样品液滴。
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