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    METHODS AND COMPOSITIONS FOR MULTIVALENT BINDING AND METHODS FOR MANUFACTURE OF RAPID DIAGNOSTIC TESTS
    2.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIVALENT BINDING AND METHODS FOR MANUFACTURE OF RAPID DIAGNOSTIC TESTS 审中-公开
    用于多重结合的方法和组合物和用于制造快速诊断测试的方法

    公开(公告)号：US20100143905A1

    公开(公告)日：2010-06-10

    申请号：US12450532

    申请日：2008-03-28

    申请人： Michael J. Lane ,  Jerrie Gavalchin ,  Brian D. Faldasz

    发明人： Michael J. Lane ,  Jerrie Gavalchin ,  Brian D. Faldasz

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/68 ,  B01L3/5023 ,  B01L2200/16 ,  B01L2300/0825

    摘要： The invention provides reagents and methods for multivalent binding and quantitative capture of components in a sample. In one aspect, reagents and methods for diagnostic assay for antigen, ligand, binding agent, or antibody are provided. Compositions of a non-natural or deliberately constructed nucleic acid-like polymeric scaffold are provided, to which multiple antibodies, peptides or other binding agents can be affixed by hybridization of a oligonucleotide: binding agent complex such that the nucleic acid: binding agent construction displays multivalent behavior when interacting with a multivalent analyte. Methods for constructing and using the scaffolds are described. Such compositions may include assembly of mixed specificity binding agents such that the composition displays multivalent binding behavior against a target containing mixed analytes which can be bound by the construct to effect a binding affinity increase such as is observed in avidity reagents against single analytes expressed multiply on the target analyte. A manufacturing method for producing rapid diagnostic assays in a decentralized manner is also described. The method generates net economic advantages over conventional diagnostic manufacturing practices.

    摘要翻译： 本发明提供用于样品中组分多价结合和定量捕获的试剂和方法。 在一个方面，提供了用于抗原，配体，结合剂或抗体的诊断测定的试剂和方法。 提供了非天然或故意构建的类似核酸的聚合物支架的组合物，通过寡核苷酸：结合剂复合物的杂交可以将多个抗体，肽或其它结合剂粘附到其上，使得核酸：结合剂结构显示 与多价分析物相互作用时的多价行为。 描述构建和使用支架的方法。 这样的组合物可以包括组合混合的特异性结合剂，使得组合物显示针对含有混合分析物的靶的多价结合行为，所述混合分析物可以被构建体结合以实现结合亲和力增加，例如针对单个分析物的亲合力试剂 目标分析物。 还描述了以分散方式产生快速诊断测定法的制造方法。 该方法比传统诊断制造实践产生净经济优势。

    Signal amplification with lollipop probes
    6.
    发明授权
    Signal amplification with lollipop probes 有权
    用棒棒糖探针进行信号放大

    公开(公告)号：US06686157B2

    公开(公告)日：2004-02-03

    申请号：US09897259

    申请日：2001-07-02

    申请人： David C. Ward ,  Patricia Bray-Ward ,  Michael J. Lane ,  Gyanendra Kumar

    发明人： David C. Ward ,  Patricia Bray-Ward ,  Michael J. Lane ,  Gyanendra Kumar

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6816 ,  C12Q2561/125 ,  C12Q2525/313 ,  C12Q2535/131

    摘要： Disclosed is a method and compositions for the sensitive detection of the amount and location of specific nucleic acid sequences. The method makes use of a branched oligomer, referred to as a lollipop oligomer, that has a tail portion, a right arm portion, and a left arm portion. These three components are joined at a common junction making a three-tailed structure. The two arms each end with sequences complementary to adjacent sequences in a target sequence. This allows the right and left arms to be ligated together when the oligomer is hybridized to the target sequence, thus topologically linking the oligomer to the target sequence. The tail portion can then be detected at the location of the target sequence.

    摘要翻译： 公开了用于灵敏检测特定核酸序列的量和位置的方法和组合物。 该方法使用称为棒棒糖低聚物的分支低聚物，其具有尾部，右臂部分和左臂部分。 这三个部件在共同的连接处连接成三尾结构。 两个臂各自以目标序列中与相邻序列互补的序列结束。 这使得当低聚物与靶序列杂交时，右臂和左臂连接在一起，从而将低聚物拓扑连接到靶序列。 然后可以在目标序列的位置检测尾部。

    Method of preparing DNA sequences with known ligand binding characteristics
    8.
    发明授权
    Method of preparing DNA sequences with known ligand binding characteristics 失效
    制备具有已知配体结合特征的DNA序列的方法

    公开(公告)号：US5593834A

    公开(公告)日：1997-01-14

    申请号：US427863

    申请日：1995-04-26

    申请人： Michael J. Lane ,  Albert S. Benight ,  Brian D. Faldasz

    发明人： Michael J. Lane ,  Albert S. Benight ,  Brian D. Faldasz

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34 ,  C12Q1/70

    CPC分类号： C12Q1/6832 ,  C12Q1/6811 ,  C12Q1/6823

    摘要： A method for determining at least one DNA sequence that can be employed as a flanking sequence to a DNA binding site for a ligand, wherein the DNA binding site is located within a preselected DNA sequence, to provide a relative increase, decrease, or approximate equality in binding constant of the ligand for its DNA binding site, or relative composite reaction rate for the ligand and a DNA sequence. Also provided are applications of the method to determine DNA primers useful in conjunction with DNA amplification to detect the presence or absence of DNA sequences and to diagnose diseases related to viral DNA, such as human immunodeficiency virus.

    摘要翻译： 用于测定可以用作配体的DNA结合位点的侧翼序列的至少一个DNA序列的方法，其中所述DNA结合位点位于预选的DNA序列内，以提供相对增加，减少或近似相等 在配体的DNA结合位点的结合常数或配体和DNA序列的相对复合反应速率。 还提供了用于确定与DNA扩增结合使用的DNA引物以检测DNA序列的存在或不存在并诊断与病毒DNA相关的疾病（例如人类免疫缺陷病毒）的方法的应用。

    Electrical equipment
    9.
    发明授权
    Electrical equipment 失效
    电子设备

    公开(公告)号：US4434402A

    公开(公告)日：1984-02-28

    申请号：US291692

    申请日：1981-08-10

    申请人： Michael J. Lane

    发明人： Michael J. Lane

    IPC分类号： F02P15/00 ,  F02P17/12 ,  H03K3/57 ,  G01R31/22

    CPC分类号： H03K3/57 ,  F02P15/003 ,  F02P17/12

    摘要： An igniter test unit produces a square wave voltage that is applied across a parallel circuit comprising a first capacitor and a series arrangement of a second capacitor and diodes. An inductance and a thyristor are connected across the first capacitor, in series. When the thyristor is rendered conductive the voltage across the diodes becomes the sum of the voltages across the capacitors, this voltage being applied across the igniter. Switching of the thyristor is controlled such that the voltage is applied to the igniter in increasing steps of 25 volts until the breakdown occurs at the same voltage five times in succession. The voltage across the igniter is determined by applying it across a capacitive divider, one capacitor being discharged in steps, and the number of steps required to reduce the voltage to a reference value is counted.

    摘要翻译： 点火器测试单元产生施加在包括第一电容器和第二电容器和二极管的串联布置的并联电路上的方波电压。 电感和晶闸管串联连接在第一电容器两端。 当晶闸管导通时，二极管两端的电压成为电容器两端的电压之和，该电压施加在点火器上。 控制晶闸管的切换，使得电压以25伏的增加步长施加到点火器，直到在相同的电压下连续发生五次击穿。 点火器两端的电压通过将其施加在电容分压器上，一个电容器逐步放电来确定，并且将电压降低到参考值所需的步数被计数。

    Lateral flow based methods and assays for rapid and inexpensive diagnostic tests
    10.
    发明授权
    Lateral flow based methods and assays for rapid and inexpensive diagnostic tests 有权
    基于侧向流的方法和测定用于快速和便宜的诊断测试

    公开(公告)号：US08900850B2

    公开(公告)日：2014-12-02

    申请号：US12807978

    申请日：2010-09-17

    申请人： Michael J. Lane ,  Brian D. Faldasz ,  Jerrie Gavalchin

    发明人： Michael J. Lane ,  Brian D. Faldasz ,  Jerrie Gavalchin

    IPC分类号： C12M1/00 ,  C12M1/34 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/542 ,  C07H21/02 ,  G01N21/75 ,  B01L3/00 ,  G01N33/569 ,  G01N33/558

    CPC分类号： G01N33/558 ,  G01N33/56972

    摘要： The invention provides reagents and methods for lateral flow assays and quantitative capture or determination of components, including cells, in a sample. In one aspect, reagents and methods for diagnostic assay are provided. In one embodiment an assay for determining T cell numbers, particularly a CD2+ CD4+ T cell assay is provided. A manufacturing method for producing rapid diagnostic assays in a decentralized manner is also described. The method generates net economic advantages over conventional diagnostic manufacturing practices.

    摘要翻译： 本发明提供用于横向流动测定和定量捕获或测定样品中组分（包括细胞）的试剂和方法。 在一个方面，提供用于诊断测定的试剂和方法。 在一个实施方案中，提供了用于确定T细胞数目，特别是CD2 + CD4 + T细胞测定的测定法。 还描述了以分散方式产生快速诊断测定的制造方法。 该方法比传统诊断制造实践产生净经济优势。