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1.发明申请Automatic cell cultivation apparatus utilizing autoclave sterilization and method for using the same 有权使用高压灭菌器的自动细胞培养装置及其使用方法公开(公告)号：US20060151185A1
公开(公告)日：2006-07-13
申请号：US11257950
申请日：2005-10-25
IPC分类号： A01B63/111
CPC分类号： A61L2/07 , C12M23/50 , C12M37/00 , C12M41/48 , Y10S435/809
摘要： To present an automatic cell cultivation apparatus capable of sterilizing completely including a cold storage unit and a normal temperature storage unit, without causing cross contamination, in used of cell cultivation of plural subjects. An automatic cell cultivation apparatus comprising an operation unit 11 for operating to cultivate cells of subjects, incubator units 14 for cultivating the cells, a cold storage unit 16 for storing reagents necessary for cultivation, a normal temperature storage unit 17 for storing culture tools, an inlet-outlet unit 15 for taking in and out reagents and culture tools, and a steam supply unit 22 for autoclave sterilization, in which the incubator units 14, the cold storage unit 16, the normal temperature storage unit 17, and the inlet-outlet unit 15 communicate with the operation unit 11, and have sealing doors 18a to 18d leading to the operation unit 11, filters 20a and 20b for cleaning and sterilizing the air stream supplied in the operation unit 11 are provided, and sealing doors 30a and 30b are provided between the filter units and the operation unit 11. By selecting the sealing doors 18a to 18d and opening and closing, sterilizing steam from the steam supply unit 22 is supplied into any of the sterilization required units, operation unit 11, and the incubator units 14, the cold storage unit 16, the normal temperature storage unit 17, and the inlet-outlet unit 15, communicating with the operation unit 11.
摘要翻译： 为了呈现能够完全包括冷藏单元和常温储存单元的自动细胞培养装置，而不会引起交叉污染，用于多个受试者的细胞培养。 一种自动细胞培养装置，包括用于培养受试者细胞的操作单元11，用于培养细胞的培养单元14，用于培养培养所需的试剂的冷藏单元16，用于储存培养工具的常温储存单元17， 用于取出和取出试剂和培养工具的入口出口单元15和用于高压灭菌的蒸汽供应单元22，其中培养箱单元14，冷藏单元16，常温储存单元17和入口插座 单元15与操作单元11通信，并且具有通向操作单元11的密封门18a至18d，设置用于清洁和消毒供应在操作单元11中的空气流的过滤器20a和20b，并且密封门 30a和30b设置在过滤器单元和操作单元11之间。 通过选择密封门18a至18d并打开和关闭，来自蒸汽供应单元22的灭菌蒸汽被供应到任何灭菌要求的单元，操作单元11和孵化器单元14，冷藏单元16， 常温存储单元17和入口出口单元15与操作单元11连通。
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2.发明授权Automatic cell cultivation apparatus utilizing autoclave sterilization and method for using the same 有权使用高压灭菌器的自动细胞培养装置及其使用方法公开(公告)号：US07883887B2
公开(公告)日：2011-02-08
申请号：US11257950
申请日：2005-10-25
CPC分类号： A61L2/07 , C12M23/50 , C12M37/00 , C12M41/48 , Y10S435/809
摘要： To present an automatic cell cultivation apparatus capable of sterilizing completely including a cold storage unit and a normal temperature storage unit, without causing cross contamination, in used of cell cultivation of plural subjects. An automatic cell cultivation apparatus comprising an operation unit 11 for operating to cultivate cells of subjects, incubator units 14 for cultivating the cells, a cold storage unit 16 for storing reagents necessary for cultivation, a normal temperature storage unit 17 for storing culture tools, an inlet-outlet unit 15 for taking in and out reagents and culture tools, and a steam supply unit 22 for autoclave sterilization, in which the incubator units 14, the cold storage unit 16, the normal temperature storage unit 17, and the inlet-outlet unit 15 communicate with the operation unit 11, and have sealing doors 18a to 18d leading to the operation unit 11, filters 20a and 20b for cleaning and sterilizing the air stream supplied in the operation unit 11 are provided, and sealing doors 30a and 30b are provided between the filter units and the operation unit 11. By selecting the sealing doors 18a to 18d and opening and closing, sterilizing steam from the steam supply unit 22 is supplied into any of the sterilization required units, operation unit 11, and the incubator units 14, the cold storage unit 16, the normal temperature storage unit 17, and the inlet-outlet unit 15, communicating with the operation unit 11.
摘要翻译： 为了呈现能够完全包括冷藏单元和常温储存单元的自动细胞培养装置，而不会引起交叉污染，用于多个受试者的细胞培养。 一种自动细胞培养装置，包括用于培养受试者细胞的操作单元11，用于培养细胞的培养单元14，用于培养培养所需的试剂的冷藏单元16，用于储存培养工具的常温储存单元17， 用于取出和取出试剂和培养工具的入口 - 出口单元15和用于高压灭菌的蒸汽供应单元22，其中培养箱单元14，冷藏单元16，常温储存单元17和入口 单元15与操作单元11连通，并且具有通向操作单元11的密封门18a至18d，设置用于清洁和消毒在操作单元11中供应的空气流的过滤器20a和20b，并且密封门30a和30b是 设置在过滤器单元和操作单元11之间。通过选择密封门18a至18d并打开和关闭来自蒸汽供应单元22的蒸汽灭菌， 与操作单元11和培养箱单元14，冷藏单元16，常温存储单元17和入口出口单元15中的任一个进行通信。
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公开(公告)号：US07816128B2
公开(公告)日：2010-10-19
申请号：US11287965
申请日：2005-11-28
CPC分类号： C12M23/50 , C12M41/48 , Y10S483/901 , Y10T74/2036
摘要： An automatic cell cultivation apparatus is provided which is capable of conveying the culture container, exchanging the culture medium, and operating other jobs promptly by a robot. The automatic cell cultivation apparatus has a cultivation apparatus main body for cultivating cells in a culture container, and the cultivation apparatus main body is equipped with one or more incubator accommodating the culture container, a chemical suction machine for sucking chemicals in the culture container, and a multijoint robot for conveying the culture container, the multijoint robot having functions of conveying the culture container, detaching and attaching the lid of the culture container, and inclining the container when sucking chemicals in the culture container, so that cell cultivation operations can be promptly carried out by only one robot.
摘要翻译： 提供一种自动细胞培养装置，其能够输送培养容器，交换培养基，并且由机器人迅速地进行其他作业。 自动细胞培养装置具有栽培培养容器中培养细胞的培养装置主体，培养装置主体装有容纳培养容器的一个或多个培养箱，用于在培养容器中吸取化学物质的化学吸引机，以及 用于输送培养容器的多关节机器人，具有输送培养容器的功能的多关节机器人，分离和附着培养容器的盖子，以及在将化学品吸入培养容器时倾斜容器，从而可以及时地进行细胞培养操作 仅由一个机器人执行。
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公开(公告)号：US20060115889A1
公开(公告)日：2006-06-01
申请号：US11287965
申请日：2005-11-28
IPC分类号： C12M1/36
CPC分类号： C12M23/50 , C12M41/48 , Y10S483/901 , Y10T74/2036
摘要： An automatic cell cultivation apparatus is provided which is capable of conveying the culture container, exchanging the culture medium, and operating other jobs promptly by a robot. The automatic cell cultivation apparatus has a cultivation apparatus main body for cultivating cells in a culture container, and the cultivation apparatus main body is equipped with one or more incubator accommodating the culture container, a chemical suction machine for sucking chemicals in the culture container, and a multijoint robot for conveying the culture container, the multijoint robot having functions of conveying the culture container, detaching and attaching the lid of the culture container, and inclining the container when sucking chemicals in the culture container, so that cell cultivation operations can be promptly carried out by only one robot.
摘要翻译： 提供一种自动细胞培养装置，其能够输送培养容器，交换培养基，并且由机器人迅速地进行其他作业。 自动细胞培养装置具有栽培培养容器中培养细胞的培养装置主体，培养装置主体装有容纳培养容器的一个或多个培养箱，用于在培养容器中吸取化学物质的化学吸引机，以及 用于输送培养容器的多关节机器人，具有输送培养容器的功能的多关节机器人，分离和附着培养容器的盖子，以及在将化学品吸入培养容器时倾斜容器，从而可以及时地进行细胞培养操作 仅由一个机器人执行。
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5.发明授权Method and apparatus for discriminating undifferentiated pluripotent stem cells, and automated culture method and system 有权鉴别未分化多能干细胞的方法和装置，以及自动培养方法和系统公开(公告)号：US09008406B2
公开(公告)日：2015-04-14
申请号：US13500626
申请日：2010-10-07
CPC分类号： G01N33/5073 , G06K9/0014 , G06K9/00147
摘要： Provided are a method capable of discriminating between a colony of differentiated pluripotent stem cells and a colony of undifferentiated pluripotent stem cells, and a method for automatically culturing undifferentiated pluripotent stem cells and a system thereof.A differentiated colony composed of differentiated pluripotent stem cells, an undifferentiated colony composed of undifferentiated pluripotent stem cells and a multilayered colony having pluripotent stem cells stacked in multiple layers are discriminated by processing a photo image of a colony including pluripotent stem cells in a culture vessel. Specifically, a colony having a luminance higher than a first threshold A is determined as a differentiated colony, a colony having a luminance equal to or lower than the first threshold A and equal to or higher than a second threshold B is determined as an undifferentiated colony, and a colony having a luminance lower than the second threshold B is determined as a multilayered colony.
摘要翻译： 提供了能够区分分化多能干细胞的集落和未分化多能干细胞的集落的方法，以及用于自动培养未分化的多能干细胞的方法及其系统。 由分化多能干细胞组成的分化集落，由未分化多能干细胞组成的未分化菌落和多层堆叠多层干细胞的多层菌落通过在培养容器中加工包含多能干细胞的菌落的照片来鉴别。 具体而言，将亮度高于第一阈值A的菌落确定为差异菌落，将具有等于或低于第一阈值A且等于或高于第二阈值B的亮度的菌落确定为未分化菌落 ，并且将具有低于第二阈值B的亮度的菌落确定为多层菌落。
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6.发明授权Method and device for identifying multipotent stem cell colony, and method and device for automatically culturing multipotent stem cells 有权用于识别多能干细胞集落的方法和装置，以及用于自动培养多能干细胞的方法和装置公开(公告)号：US08977031B2
公开(公告)日：2015-03-10
申请号：US13805767
申请日：2011-06-22
申请人： Keizo Watakabe , Takashi Sakurai , Osamu Ohji , Katsumi Nakashima
发明人： Keizo Watakabe , Takashi Sakurai , Osamu Ohji , Katsumi Nakashima
CPC分类号： C12M41/46
摘要： A method and device achieves identification on whether a colony on a cultivation container is differentiated or undifferentiated on an occasion of cultivation of multipotent stem cell, and a method and device achieves a rapid culture of undifferentiated multipotent stem cells. Undifferentiated colony containing only undifferentiated multipotent stem cells is identified from other types of colonies by means of the circularity C of the colony in an image-processed taken image of the inside of cultivation container of a multipotent stem cell. More specifically, when a first threshold of the circularity of the colony is C1, and a second predetermined threshold is C2 (C1
摘要翻译： 一种方法和装置能够确定培养容器上的菌落在培养多能干细胞的情况下是分化还是未分化，并且方法和装置实现未分化多能干细胞的快速培养。 通过在多能干细胞培养容器内部的图像处理的拍摄图像中的菌落的圆形度C，从其他类型的菌落中鉴定到仅含有未分化的多能干细胞的未分化菌落。 更具体地说，当菌落的圆形度的第一阈值为C1，第二预定阈值为C2（C1
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7.发明申请Method and Apparatus for Discriminating Undifferentiated Pluripotent Stem Cells, and Automated Culture Method and System 有权用于鉴别未分化多能干细胞的方法和装置，以及自动培养方法和系统公开(公告)号：US20120315620A1
公开(公告)日：2012-12-13
申请号：US13500626
申请日：2010-10-07
CPC分类号： G01N33/5073 , G06K9/0014 , G06K9/00147
摘要： Provided are a method capable of discriminating between a colony of differentiated pluripotent stem cells and a colony of undifferentiated pluripotent stem cells, and a method for automatically culturing undifferentiated pluripotent stem cells and a system thereof.A differentiated colony composed of differentiated pluripotent stem cells, an undifferentiated colony composed of undifferentiated pluripotent stem cells and a multilayered colony having pluripotent stem cells stacked in multiple layers are discriminated by processing a photo image of a colony including pluripotent stem cells in a culture vessel. Specifically, a colony having a luminance higher than a first threshold A is determined as a differentiated colony, a colony having a luminance equal to or lower than the first threshold A and equal to or higher than a second threshold B is determined as an undifferentiated colony, and a colony having a luminance lower than the second threshold B is determined as a multilayered colony.
摘要翻译： 提供了能够区分分化多能干细胞的集落和未分化多能干细胞的集落的方法，以及用于自动培养未分化的多能干细胞的方法及其系统。 由分化多能干细胞组成的分化集落，由未分化多能干细胞组成的未分化菌落和多层堆叠多层干细胞的多层菌落通过在培养容器中加工包含多能干细胞的菌落的照片来鉴别。 具体而言，将亮度高于第一阈值A的菌落确定为差异菌落，将具有等于或低于第一阈值A且等于或高于第二阈值B的亮度的菌落确定为未分化菌落 ，并且将具有低于第二阈值B的亮度的菌落确定为多层菌落。
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8.发明授权Method of reclaiming crosslinked rubber, and molded article of reclaimed rubber 有权回收交联橡胶的方法和再生橡胶的成型品公开(公告)号：US06777453B1
公开(公告)日：2004-08-17
申请号：US10009179
申请日：2002-05-03
申请人： Mitsumasa Matsushita , Makoto Mouri , Hirotaka Okamoto , Kenzo Fukumori , Norio Sato , Toru Yoshida , Masahito Fukuta , Hidenobu Honda , Katsumi Nakashima , Tamotsu Watanabe , Yasuyuki Suzuki , Masao Owaki
发明人： Mitsumasa Matsushita , Makoto Mouri , Hirotaka Okamoto , Kenzo Fukumori , Norio Sato , Toru Yoshida , Masahito Fukuta , Hidenobu Honda , Katsumi Nakashima , Tamotsu Watanabe , Yasuyuki Suzuki , Masao Owaki
IPC分类号： C08J1104
CPC分类号： C08J11/10 , B29B17/00 , B29B17/02 , B29B2017/0293 , B29B2017/0464 , B29K2021/00 , C08J2321/00 , Y02W30/62 , Y02W30/622 , Y02W30/625 , Y02W30/68 , Y02W30/702
摘要： The object of the present invention is to provide a method of reclaiming crosslinked rubber wherein a reduction of qualities due to generation of decomposed products hardly occurs, and a molding of reclaimed rubber. In a reclamation step of reclaiming crosslinked rubber 10 by applying shear stress thereto and/or in a subsequent step in the present invention, a degasification carrier is introduced and decomposed products in the crosslinked rubber 10 are removed together with the degasification carrier. Also the disclosed is a molding of reclaimed rubber prepared by re-crosslinking reclaimed rubber obtained by this method.
摘要翻译： 本发明的目的是提供一种回收交联橡胶的方法，其中难以产生分解产物的产生的质量降低，以及再生橡胶的成型。在通过施加剪切应力向回收交联橡胶10的回收步骤中 和/或在本发明的后续步骤中，引入脱气载体，并与脱气载体一起除去交联橡胶10中的分解产物。 还公开了通过该方法获得的再交联再生橡胶制备的再生橡胶的成型体。
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公开(公告)号：US06207723B1
公开(公告)日：2001-03-27
申请号：US09236445
申请日：1999-01-25
申请人： Mitsumasa Matsushita , Makoto Mouri , Hirotaka Okamoto , Norio Sato , Yasuyuki Suzuki , Masao Owaki , Noriyuki Suzuki , Katsumi Nakashima , Hidenobu Honda , Toru Yoshida , Katsumasa Takeuchi
发明人： Mitsumasa Matsushita , Makoto Mouri , Hirotaka Okamoto , Norio Sato , Yasuyuki Suzuki , Masao Owaki , Noriyuki Suzuki , Katsumi Nakashima , Hidenobu Honda , Toru Yoshida , Katsumasa Takeuchi
IPC分类号： C08J1104
CPC分类号： C08J11/12 , C08J2321/00 , C08L7/00 , C08L9/00 , C08L11/00 , C08L17/00 , C08L23/06 , C08L23/12 , C08L23/16 , C08L23/22 , C08L25/06 , C08L25/10 , C08L29/14 , C08L33/20 , C08L67/00 , C08L69/00 , C08L71/03 , C08L75/04 , C08L77/00 , C08L83/00 , Y02W30/703 , C08L2666/02
摘要： The present invention provides a rubber composition containing a reclaimed rubber and a method for producing the same. A rubber molded article having excellent rubber properties can be obtained and the recycling of a used rubber product can be promoted by the present invention. A rubber composition of the present invention is a blend comprising a claimed rubber obtained by applying a heat and a shear stress to a vulcanized rubber, a non-vulcanized virgin rubber or/and a thermoplastic resin, wherein the reclaimed rubber contains 40% by weight or more of a residual toluene-insoluble gel component and the network chain density of the rubber in the gel component is 1/20 to 1/4 based on the network chain density of the rubber of the vulcanized rubber.
摘要翻译： 本发明提供一种含有再生橡胶的橡胶组合物及其制造方法。 本发明可以得到具有优异的橡胶性能的橡胶模制品，并且可以通过本发明促进废旧橡胶产品的再循环。本发明的橡胶组合物是包含通过施加热和剪切应力获得的要求保护的橡胶的共混物 涉及硫化橡胶，未硫化的原始橡胶或/和热塑性树脂，其中回收的橡胶含有40重量％以上的残留的甲苯不溶性凝胶成分，凝胶成分中的橡胶的网络链密度为 基于硫化橡胶的橡胶的网络链密度为1/20至1/4。
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公开(公告)号：US6132847A
公开(公告)日：2000-10-17
申请号：US186881
申请日：1998-11-06
IPC分类号： C08J9/06 , B29C47/00 , B29K21/00 , B29K23/00 , B29K105/04 , C08J9/00 , C08J9/10 , C08K9/04 , C08L23/16
CPC分类号： C08J9/0066 , C08J9/10 , C08J2323/16 , Y10T428/24504 , Y10T428/269
摘要： A formed rubber extruded product which is formed of a vulcanizate of a rubber blend comprising a sulfur-vulcanizable ethylene-propylene-type rubber (EPDM) as a starting rubber and a pyrolytic foaming agent. The vulcanizate has a specific gravity of from 0.9 to 1.0 and a surface roughness (RZD) of 14 micrometers or less. The rubber blend contains the pyrolytic foaming agent in the form of an inorganic powder-supported foaming agent. A particle diameter of the inorganic powder in the inorganic powder-supported foaming agent is approximately 9 micrometers or less, and a particle diameter of the pyrolytic foaming agent is approximately 8 micrometers or less. Further, crystalline PE is contained in an amount of from 6 to 30 parts per 100 parts of EPDM. The foamed rubber extruded product of the present invention can be produced by the extrusion at a high rate.
摘要翻译： 一种形成的橡胶挤出产品，由橡胶混合物硫化橡胶制成，该橡胶混合物含有作为起始橡胶的可硫化乙烯 - 丙烯类橡胶（EPDM）和热解发泡剂。 硫化橡胶的比重为0.9〜1.0，表面粗糙度（RZD）为14微米以下。 橡胶混合物含有无机粉末负载发泡剂形式的热解发泡剂。 无机粉末负载发泡剂中的无机粉末的粒径为约9微米以下，热解发泡剂的粒径为约8微米以下。 此外，结晶PE的含量为每100份EPDM为6〜30份。 本发明的发泡橡胶挤出产品可以通过高速挤出制造。
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