公开(公告)号：US20020155586A1

公开(公告)日：2002-10-24

申请号：US10163835

申请日：2002-06-05

申请人： Nanogen, Inc.

发明人： Jing Cheng ,  Lei Wu ,  Michael J. Heller ,  Edward L. Sheldon ,  Jonathan M. Diver ,  James P. O'Connell ,  Dan Smolko ,  Shila Jalali ,  David Willoughby

IPC分类号： C12M001/34

CPC分类号： G11C13/0019 ,  B01D57/02 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00689 ,  B01J2219/00713 ,  B01J2219/00722 ,  B01J2219/00853 ,  B01J2219/00914 ,  B01L3/5027 ,  B01L3/502753 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0496 ,  B01L2400/0644 ,  B03C5/026 ,  B03C5/028 ,  B82Y5/00 ,  B82Y10/00 ,  C07H21/00 ,  C07K1/045 ,  C12N13/00 ,  C12Q1/6806 ,  C12Q1/6825 ,  C12Q1/6832 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  G01N27/44743 ,  G11C13/0014 ,  G11C13/04 ,  G11C19/00 ,  H01L25/50 ,  H01L2924/0002 ,  C12Q2565/515 ,  C12Q2565/607 ,  C12Q2565/629 ,  C12Q2525/107 ,  C12Q2531/119 ,  C12Q2531/101 ,  C12Q2531/113 ,  H01L2924/00

摘要： We have performed separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells were examined by staining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. We have also released DNA and RNA from the isolated cells electronically and detected specific marker sequences by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the construction of a nulllaboratory-on-a-chipnull system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.