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    Microchemical nanofactories
    2.
    发明申请
    Microchemical nanofactories 失效
    微化学纳米工艺

    公开(公告)号：US20050220681A1

    公开(公告)日：2005-10-06

    申请号：US11086074

    申请日：2005-03-21

    申请人： Chih-Hung Chang ,  Brian Paul ,  Vincent Remcho

    发明人： Chih-Hung Chang ,  Brian Paul ,  Vincent Remcho

    IPC分类号： B01F5/04 ,  B01F13/00 ,  B01J8/00 ,  B01J19/00 ,  C08G83/00

    CPC分类号： B01F5/0475 ,  B01F5/0485 ,  B01F13/0062 ,  B01F13/0066 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00822 ,  B01J2219/00824 ,  B01J2219/00833 ,  B01J2219/00844 ,  B01J2219/00853 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00912 ,  B01J2219/00914 ,  B01J2219/00916 ,  B01J2219/0097 ,  B01J2219/00972 ,  C08G83/003 ,  F28F2210/02 ,  Y10T428/24562 ,  Y10T428/24612 ,  Y10T428/24661 ,  Y10T428/24744

    摘要： Embodiments of an apparatus, system, and method for chemical synthesis and/or analysis are disclosed. One embodiment of a disclosed apparatus comprises a laminated, microfluidic structure defining a reactor and a separator. Such apparatuses, or portions thereof, generally have dimensions ranging from about 1 micrometer to about 100 micrometers. To implement synthetic processes, disclosed embodiments of the apparatus generally include at least one valve, and often plural, selectively actuatable valves. Detectors, including optical detectors, also can be used to detect product and other materials as they flow by, or are otherwise presented to, the detector. Individual apparatuses may be coupled both in series and in parallel to form a system for making chemical compounds. The apparatus is particularly useful for making compounds requiring iterative reaction schemes, and further can be used to make compounds having morphological structures that resemble the morphology of the apparatus itself, such as dendrimers.

    摘要翻译： 公开了用于化学合成和/或分析的装置，系统和方法的实施例。 公开的装置的一个实施例包括限定反应器和分离器的层压的微流体结构。 这种装置或其部分通常具有约1微米至约100微米的尺寸。 为了实现合成过程，所公开的设备的实施例通常包括至少一个阀，并且通常是多个可选择性致动的阀。 检测器，包括光学检测器，也可用于检测产品和其他材料，因为它们流过或以其他方式呈现给检测器。 单个装置可以串联耦合并联，以形成用于制备化合物的系统。 该装置对于制备需要迭代反应方案的化合物特别有用，并且还可用于制备具有类似于装置本身形态的形态结构的化合物，例如树枝状大分子。

    Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
    9.
    发明授权
    Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis 有权
    通过介电电泳在生物电子芯片上无生物颗粒的分离

    公开(公告)号：US06280590B1

    公开(公告)日：2001-08-28

    申请号：US09548522

    申请日：2000-04-13

    申请人： Jing Cheng ,  Edward L. Sheldon, III ,  Lei Wu ,  James P. O'Connell

    发明人： Jing Cheng ,  Edward L. Sheldon, III ,  Lei Wu ,  James P. O'Connell

    IPC分类号： G01N2726

    CPC分类号： B01L3/5027 ,  B01D57/02 ,  B01J19/0093 ,  B01J2219/00853 ,  B01J2219/00914 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0496 ,  B01L2400/0644 ,  B03C5/026 ,  B03C5/028 ,  C12N13/00 ,  G01N27/44743 ,  H01L29/6656

    摘要： The present invention comprises devices and methods for performing channel-less separation of cell particles by dielectrophoresis, DC high voltage-pulsed electronic lysis of separated cells, separation of desired components from crude mixtures such as cell lysates, and/or enzymatic reaction of such lysates, all of which can be conducted on a single bioelectronic chip. A preferred embodiment of the present invention comprises a cartridge (10) including a microfabricated silicon chip (12) on a printed circuit board (14) and a flow cell (16) mounted to the chip (12) to form a flow chamber. The cartridge (10) also includes output pins (22) for electronically connecting the cartridge (10) to an electronic controller. The chip (12) includes a plurality of circular microelectrodes (24) which are preferably coated with a protective permeation layer which prevents direct contact between any electrode and a sample introduced into the flow chamber. The permeation layer also helps to reduce cell adhesion at field minima, and enables immobilization of specific antibodies for specific cell capture. Specific cells from various cell mixtures were separated, lysed, and enzymatically digested on the chip.

    摘要翻译： 本发明包括用于通过介电电泳，DC分离细胞的DC高压脉冲电离裂解，细胞裂解物等粗制混合物分离所需组分和/或这些裂解物的酶反应来进行细胞颗粒的无通道分离的装置和方法 所有这些都可以在单个生物电子芯片上进行。 本发明的优选实施例包括在印刷电路板（14）上包括微细硅芯片（12）的盒（10）和安装到芯片（12）上以形成流动室的流动池（16）。 盒（10）还包括用于将盒（10）电连接到电子控制器的输出销（22）。 芯片（12）包括多个圆形微电极（24），其优选地涂覆有保护性渗透层，其防止任何电极和引入流动室中的样品之间的直接接触。 渗透层还有助于减少最小场的细胞粘附，并且能够固定特异性抗体用于特异性细胞捕获。 分离来自各种细胞混合物的特异性细胞，裂解，并在芯片上酶消化。