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公开(公告)号：US20240352447A1
公开(公告)日：2024-10-24
申请号：US18750621
申请日：2024-06-21
发明人： Zhenyu Zhu , Aine Quimby , Shuang-Yong Xu , Shengxi Guan , Hua Wei , Penghua Zhang , Dapeng Sun , Siu-hong Chan
CPC分类号： C12N15/1058 , C12N9/16 , C12N9/22 , C12N15/1034 , C12N15/1086
摘要： Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor.
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公开(公告)号：US20240352437A1
公开(公告)日：2024-10-24
申请号：US18762074
申请日：2024-07-02
发明人： Zhenyu Zhu , Aine Quimby , Shengxi Guan , Dapeng Sun , Yishu Huang , Xuhui Lai , Siu-hong Chan , Xianghui Li , Shuang-Yong Xu , Chunhua Zhang
CPC分类号： C12N9/22 , C12N9/16 , C12Q1/34 , C12Q1/44 , C12Q1/68 , C12Y301/21004 , G01N2333/916 , G01N2333/922
摘要： Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
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公开(公告)号：US20180171328A1
公开(公告)日：2018-06-21
申请号：US15882603
申请日：2018-01-29
发明人： Zhenyu Zhu , Aine Quimby , Shuang-Yong Xu , Shengxi Guan , Hua Wei , Penghua Zhang , Dapeng Sun , Siu-hong Chan
CPC分类号： C12N15/1058 , C12N9/16 , C12N9/22 , C12N15/1034 , C12N15/1086
摘要： Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor.
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公开(公告)号：US09267117B2
公开(公告)日：2016-02-23
申请号：US13826395
申请日：2013-03-14
发明人： Shengxi Guan , Nan Dai , Zhenyu Zhu , Ivan R. Correa, Jr. , Aine Quimby , Janine Borgaro
IPC分类号： C12Q1/68 , C12N9/02 , C07H19/06 , C07H21/00 , C12P19/18 , C12P19/30 , C12P19/34 , C12N9/10 , C12N9/22 , C07K14/47
CPC分类号： C12N9/0069 , C07H19/06 , C07H21/00 , C07K14/47 , C07K2319/80 , C07K2319/81 , C12N9/1051 , C12N9/22 , C12P19/18 , C12P19/30 , C12P19/34 , C12Q1/68 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/683 , Y02P20/52 , C12Q2521/301 , C12Q2523/115 , C12Q2525/101 , C12Q2527/125 , C12Q2537/164
摘要： Methods, compositions and kits for selectively altering and detecting modified cytosine residues are provided.
摘要翻译： 提供了用于选择性地改变和检测修饰的胞嘧啶残基的方法，组合物和试剂盒。
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公开(公告)号：US20140154780A1
公开(公告)日：2014-06-05
申请号：US14137660
申请日：2013-12-20
发明人： Zhenyu Zhu , Aine Quimby , Shengxi Guan , Dapeng Sun , Yishu Huang , Xuhui Lai , Siu-hong Chan , Xianghui Li , Shuang-Yong Xu , Chunhua Zhang
CPC分类号： C12N9/22 , C12N9/16 , C12Q1/34 , C12Q1/44 , C12Q1/68 , C12Y301/21004 , G01N2333/916 , G01N2333/922
摘要： Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
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公开(公告)号：US20140127678A1
公开(公告)日：2014-05-08
申请号：US13826395
申请日：2013-03-14
发明人： Shengxi Guan , Nan Dai , Zhenyu Zhu , Ivan R. Correa, Jr. , Aine Quimby , Janine Borgaro
IPC分类号： C12Q1/68
CPC分类号： C12N9/0069 , C07H19/06 , C07H21/00 , C07K14/47 , C07K2319/80 , C07K2319/81 , C12N9/1051 , C12N9/22 , C12P19/18 , C12P19/30 , C12P19/34 , C12Q1/68 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/683 , Y02P20/52 , C12Q2521/301 , C12Q2523/115 , C12Q2525/101 , C12Q2527/125 , C12Q2537/164
摘要： Methods, compositions and kits for selectively altering and detecting modified cytosine residues are provided.
摘要翻译： 提供了用于选择性地改变和检测修饰的胞嘧啶残基的方法，组合物和试剂盒。
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公开(公告)号：US20130115678A1
公开(公告)日：2013-05-09
申请号：US13736561
申请日：2013-01-08
发明人： Zhenyu Zhu , Aine Quimby , Shuang-Yong Xu , Shengxi Guan , Hua Wei , Penghua Zhang , Dapeng Sun , Siu-hong Chan
IPC分类号： C12N9/22
CPC分类号： C12N15/1058 , C12N9/16 , C12N9/22 , C12N15/1034 , C12N15/1086
摘要： Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor.
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公开(公告)号：US09988614B1
公开(公告)日：2018-06-05
申请号：US15337597
申请日：2016-10-28
发明人： Zhenyu Zhu , Aine Quimby
CPC分类号： C12N9/22 , C12N9/93 , C12P19/34 , C12Y605/01001
摘要： A composition comprising a variant BbsI restriction endonuclease having reduced star activity is provided, as well as kits and methods employing the same.
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公开(公告)号：US09856464B2
公开(公告)日：2018-01-02
申请号：US15133093
申请日：2016-04-19
发明人： Zhenyu Zhu , Aine Quimby , Shengxi Guan , Dapeng Sun , Yishu Huang , Xuhui Lai , Siu-hong Chan , Xianghui Li , Shuang-Yong Xu , Chunhua Zhang
CPC分类号： C12N9/22 , C12N9/16 , C12Q1/34 , C12Q1/44 , C12Q1/68 , C12Y301/21004 , G01N2333/916 , G01N2333/922
摘要： Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
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公开(公告)号：US20160289660A1
公开(公告)日：2016-10-06
申请号：US15133093
申请日：2016-04-19
发明人： Zhenyu Zhu , Aine Quimby , Shengxi Guan , Dapeng Sun , Yishu Huang , Xuhui Lai , Siu-hong Chan , Xianghui Li , Shuang-Yong Xu , Chunhua Zhang
CPC分类号： C12N9/22 , C12N9/16 , C12Q1/34 , C12Q1/44 , C12Q1/68 , C12Y301/21004 , G01N2333/916 , G01N2333/922
摘要： Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.
摘要翻译： 提供了用于工程化具有降低的星形活性的突变酶的方法和组合物，其中突变酶在特定缓冲液中具有比同一缓冲液中非突变酶的FI高的保真度指数（FI）。
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