公开(公告)号：US20230416705A1

公开(公告)日：2023-12-28

申请号：US18340083

申请日：2023-06-23

Applicant: New England Biolabs, Inc.

Inventor： Mehul Ganatra ,  Siu-hong Chan ,  Christopher H. Taron ,  G. Brett Robb

IPC: C12N9/16 ,  C12N9/12 ,  C12N9/10 ,  A61K39/245 ,  C12N7/00

CPC classification number: C12N9/16 ,  C12Y301/03033 ,  C12N9/1241 ,  C12Y207/0705 ,  C12N9/1007 ,  A61K39/245 ,  C12N7/00 ,  C12Y201/01056 ,  C12N2710/16134 ,  A61K2039/53

Abstract: The present disclosure relates to compositions, kits, and methods of making RNA vaccines having an appropriate cap structure. Systems, apparatus, compositions, and/or methods may include and/or use, in some embodiments, non-naturally occurring single-chain RNA capping enzymes. In some embodiments, an RNA capping enzyme may include an FCE variant having (a) an amino acid sequence at least 90% identical to positions 1 to 878 of SEQ ID NO: 1, and/or (b) one or more substitutions relative to SEQ ID NO: 1 at a position selected from positions corresponding to positions 215, 337, 572, 648, and 833 (e.g., a position selected from positions corresponding to position 215, 337, and 572) of SEQ ID NO: 1.

公开(公告)号：US20210054016A1

公开(公告)日：2021-02-25

申请号：US17001236

申请日：2020-08-24

Applicant: New England Biolabs, Inc.

Inventor： G. Brett Robb ,  Siu-Hong Chan ,  Bijoyita Roy

IPC: C07H19/20 ,  C07H21/04

Abstract: Provided herein is a method for efficiently capping RNA in vitro. In some embodiments the capping reaction may be done at high temperature using Vaccinia capping enzyme or a variant thereof. In other embodiments, the capping reactions may comprise a capping enzyme from a large virus of amoeba, e.g., Faustovirus, mimivirus or moumouvirus, or a variant thereof. Compositions and kits for practicing the method are also provided.

公开(公告)号：US20140357528A1

公开(公告)日：2014-12-04

申请号：US13904452

申请日：2013-05-29

Applicant: New England Biolabs, Inc.

Inventor： G. Brett Robb ,  Ryan T. Fuchs ,  Fanglei Zhuang

IPC: C12N15/10

CPC classification number: C12N15/1096

Abstract: An improved adapter design for ligation to target RNA in a library is described which enables target RNAs that would normally be under-represented or not represented at all in a cDNA library or a PCR product of the cDNA library, to be represented with average frequency.

Abstract translation: 描述了用于连接到文库中的靶RNA的改进的衔接子设计，其使得通常在cDNA文库或cDNA文库的PCR产物中通常不被低表达或不表示的靶RNA以平均频率表示。

公开(公告)号：US20240287592A1

公开(公告)日：2024-08-29

申请号：US18562953

申请日：2022-06-09

Applicant: New England Biolabs, Inc.

Inventor： Nathan Tanner ,  Ryan T. Fuchs ,  Jennifer L. Curcuru ,  G. Brett Robb

IPC: C12Q1/6844 ,  C12N9/12 ,  C12N9/22 ,  C12Q1/48 ,  C12Q1/6823

CPC classification number: C12Q1/6844 ,  C12N9/1252 ,  C12N9/22 ,  C12Q1/485 ,  C12Q1/6823 ,  C12Y207/07007 ,  C12Y207/07049

Abstract: Kits and methods are provided that utilize a mesophilic strand displacing polymerase selected from Bsu DNA polymerase (large fragment) and Klenow in Loop mediated amplification (LAMP) at temperatures in the range of 34° C.-52ºC. This contrasts with 60° C.-65° C. required for standard Bst polymerase dependent LAMP. The reduced temperature of the LAMP reaction enables the use of other proteins that are temperature sensitive in a one-step reaction. For example, a Cas protein such as Cas12a may be used with a target nucleic acid specific guide RNA and optionally a reporter oligonucleotide containing a quencher and a fluorophore or lateral flow reagents to determine the presence of pathogens in a sample.

公开(公告)号：US20230357838A1

公开(公告)日：2023-11-09

申请号：US18323143

申请日：2023-05-24

Applicant: New England Biolabs, Inc.

Inventor： Zhiyi Sun ,  Sean R. Johnson ,  Bo Yan ,  Lixin Chen ,  G. Brett Robb ,  Thomas C. Evans, JR. ,  Romualdas Vaisvila

IPC: C12Q1/6869 ,  C12N9/02 ,  C12N9/10 ,  C12N9/78

CPC classification number: C12Q1/6869 ,  C12N9/0071 ,  C12Y114/11 ,  C12N9/1051 ,  C12Y204/01027 ,  C12N9/78 ,  C12Y305/04

Abstract: Provided herein, among other things, is a method for deaminating a double-stranded nucleic acid. In some embodiments, the method may comprise contacting a double-stranded DNA substrate that comprises cytosines and a double-stranded DNA deaminase having an amino acid sequence that is at least 80% identical to any of SEQ ID NOS: 21, 40, 47, 49, 50, 55, 58, 59, 62, 63, 65, 67, 70, 71, 76, 106, 107, 110, 112, 114, 117, 163 and/or 164 to produce a deamination product that comprises deaminated cytosines. Enzymes and kits for performing the method are also provided.

公开(公告)号：US12168788B2

公开(公告)日：2024-12-17

申请号：US18340083

申请日：2023-06-23

Applicant: New England Biolabs, Inc.

Inventor： Mehul Ganatra ,  Siu-Hong Chan ,  Christopher H. Taron ,  G. Brett Robb

IPC: C12N9/16 ,  A61K39/00 ,  A61K39/245 ,  C07K14/005 ,  C12N7/00 ,  C12N9/10 ,  C12N9/12

Abstract: The present disclosure relates to compositions, kits, and methods of making RNA vaccines having an appropriate cap structure. Systems, apparatus, compositions, and/or methods may include and/or use, in some embodiments, non-naturally occurring single-chain RNA capping enzymes. In some embodiments, an RNA capping enzyme may include an FCE variant having (a) an amino acid sequence at least 90% identical to positions 1 to 878 of SEQ ID NO: 1, and/or (b) one or more substitutions relative to SEQ ID NO: 1 at a position selected from positions corresponding to positions 215, 337, 572, 648, and 833 (e.g., a position selected from positions corresponding to position 215, 337, and 572) of SEQ ID NO: 1.

公开(公告)号：US09422551B2

公开(公告)日：2016-08-23

申请号：US13904452

申请日：2013-05-29

Applicant: New England Biolabs, Inc.

Inventor： G. Brett Robb ,  Ryan T. Fuchs ,  Fanglei Zhuang

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12N15/1096

Abstract: An improved adapter design for ligation to target RNA in a library is described which enables target RNAs that would normally be under-represented or not represented at all in a cDNA library or a PCR product of the cDNA library, to be represented with average frequency.

Abstract translation: 描述了用于连接到文库中的靶RNA的改进的衔接子设计，其使得通常在cDNA文库或cDNA文库的PCR产物中通常不被低表达或不表示的靶RNA以平均频率表示。