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公开(公告)号：US08658400B2
公开(公告)日：2014-02-25
申请号：US13140255
申请日：2009-12-16
IPC分类号： C12P17/00 , C12P7/02 , C12N9/04 , C12N1/20 , C12P21/06 , C12N15/00 , C07D333/12 , C07H21/04 , C07K1/00
CPC分类号： C12P17/00 , C12N9/0006 , C12P7/02 , C12P7/22
摘要： The present invention relates to novel phenylethanol dehydrogenase mutants, to a method for the manufacture thereof; to coded nucleic acid sequences therefor, to expression cassettes, to vectors and recombinant microorganisms that contain said sequences; to a method for the biocatalytic synthesis of substituted, optically active alcohols and to the use of said mutants; and particularly to a method for manufacturing duloxetine alcohol or duloxetine, comprising a synthesis step catalyzed biocatalytic by said mutants.
摘要翻译： 本发明涉及新型苯乙醇脱氢酶突变体，涉及其制备方法; 将其编码的核酸序列，表达盒，载体和包含所述序列的重组微生物; 涉及用于生物催化合成取代的光学活性醇和使用所述突变体的方法; 特别涉及一种制造度洛西汀醇或度洛西汀的方法，其包括由所述突变体催化生物催化的合成步骤。
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公开(公告)号：US20110250655A1
公开(公告)日：2011-10-13
申请号：US13140255
申请日：2009-12-16
CPC分类号： C12P17/00 , C12N9/0006 , C12P7/02 , C12P7/22
摘要： The present invention relates to novel phenylethanol dehydrogenase mutants, to a method for the manufacture thereof; to coded nucleic acid sequences therefor, to expression cassettes, to vectors and recombinant microorganisms that contain said sequences; to a method for the biocatalytic synthesis of substituted, optically active alcohols and to the use of said mutants; and particularly to a method for manufacturing duloxetine alcohol or duloxetine, comprising a synthesis step catalyzed biocatalytic by said mutants.
摘要翻译： 本发明涉及新型苯乙醇脱氢酶突变体，涉及其制备方法; 将其编码的核酸序列，表达盒，载体和包含所述序列的重组微生物; 涉及用于生物催化合成取代的光学活性醇和使用所述突变体的方法; 特别涉及一种制造度洛西汀醇或度洛西汀的方法，其包括由所述突变体催化生物催化的合成步骤。
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公开(公告)号：US08951750B2
公开(公告)日：2015-02-10
申请号：US12744937
申请日：2008-11-28
申请人： Bernhard Hauer , Nina Schneider , Krzysztof Okrasa , Jason Micklefield , David Leys , Colin Levy
发明人： Bernhard Hauer , Nina Schneider , Krzysztof Okrasa , Jason Micklefield , David Leys , Colin Levy
IPC分类号： C12Q1/64 , G01N33/53 , C07B37/06 , C12N9/88 , C07K14/235 , C12N9/90 , C12P7/40 , C12P7/42 , C12Q1/00
CPC分类号： C12N9/88 , C07K14/235 , C07K2299/00 , C12N9/90 , C12P7/40 , C12P7/42 , C12Q1/00 , C12Y401/01075 , C12Y401/01076 , G01N2333/235 , G01N2500/00
摘要： The present invention relates to a method for the enzymatic decarboxylation of malonic acid (propanedioic acid) derivatives catalyzed by enzymes structurally and/or functionally related to arylmalonate decarboxylase (AMDase) as isolated from microorganisms of the genus Bordetella. The present invention also relates to novel enzymes with a decarboxylase activity, useful for performing the claimed method, mutants thereof, corresponding coding sequences and expression systems, methods of preparing said novel enzymes, and screening methods for obtaining further suitable enzymes also having said decarboxylase activity.
摘要翻译： 本发明涉及由结构和/或与芳基丙二酸酯脱羧酶（AMDase）功能相关的酶催化的由在博德特氏菌属微生物分离的酶催化的丙二酸（丙二酸）衍生物的酶脱羧的方法。 本发明还涉及具有脱羧酶活性的新型酶，可用于实施要求保护的方法，其突变体，相应的编码序列和表达系统，制备所述新型酶的方法，以及筛选获得还具有所述脱羧酶活性的合适酶的筛选方法 。
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4.发明申请公开(公告)号：US20130005002A1
公开(公告)日：2013-01-03
申请号：US13497985
申请日：2010-09-24
申请人： Bernhard Hauer , Thomas Friedrich , Rainer Stürmer , Nina Schneider , Susanne Krauser , Wolf-Rüdiger Krahnert
发明人： Bernhard Hauer , Thomas Friedrich , Rainer Stürmer , Nina Schneider , Susanne Krauser , Wolf-Rüdiger Krahnert
CPC分类号： C12P13/001 , C12N9/80 , C12P13/04 , C12P41/007
摘要： Process for producing optically active 3-aminocarboxylic acid ester compounds of general Formula I, and the ammonium salts thereof, in which R1 stands for alkyl, alkoxyalkyl, alkenyl, cycloalkyl, heterocycloalkyl, aryl, or hetaryl, and R2 stands for alkyl, cycloalkyl or aryl, in which an enantiomeric mixture of a simply N-acylated 3-aminocarboxylic acid ester of general formula (I.b), in which R1 and R2 have the meanings given above and R3 stands for hydrogen, alkyl, cycloalkyl or aryl, is submitted to an enantioselective deacylation by adding a polypeptide according to claim 1.
摘要翻译： 制备通式I的光学活性3-氨基羧酸酯化合物及其铵盐的方法，其中R1代表烷基，烷氧基烷基，烯基，环烷基，杂环烷基，芳基或杂芳基，R2代表烷基，环烷基或 芳基，其中通式（Ib）的简单N-酰化3-氨基羧酸酯的对映体混合物，其中R 1和R 2具有上面给出的含义，R 3代表氢，烷基，环烷基或芳基，被提交到 根据权利要求1的通过加入多肽的对映选择性脱酰作用。
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公开(公告)号：US09096841B2
公开(公告)日：2015-08-04
申请号：US13496086
申请日：2010-09-15
申请人： Nina Schneider , Bernhard Hauer , Klaus Ditrich , Maeve O'Neil , Nick Turner
发明人： Nina Schneider , Bernhard Hauer , Klaus Ditrich , Maeve O'Neil , Nick Turner
摘要： The present invention relates to a process for the biocatalytic, enantioselective production of a β-amino acid precursor from an optionally substituted dihydrouracil using a hydantoinase and/or a dihydropyrimidinase, a process for producing a β-amino acid from said precursor, a hydantoinase and its use in said process for the biocatalytic production of a β-amino acid precursor or a β-amino acid, and a method for obtaining said hydantoinase.
摘要翻译： 本发明涉及使用乙内酰脲酶和/或二氢嘧啶苷从任选取代的二氢尿嘧啶生物催化，对映选择性地生产“氨基酸”前体的方法，一种从所述前体制备一种氨基酸的方法， 乙内酰脲及其用于生物催化生产“氨基酸”前体或“氨基酸”的所述方法中的应用，以及用于获得所述乙内酰脲酶的方法。
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公开(公告)号：US20100311037A1
公开(公告)日：2010-12-09
申请号：US12744937
申请日：2008-11-28
申请人： Bernhard Hauer , Nina Schneider , Krzysztof Okrasa , Jason Micklefield , David Leys , Colin Levy
发明人： Bernhard Hauer , Nina Schneider , Krzysztof Okrasa , Jason Micklefield , David Leys , Colin Levy
CPC分类号： C12N9/88 , C07K14/235 , C07K2299/00 , C12N9/90 , C12P7/40 , C12P7/42 , C12Q1/00 , C12Y401/01075 , C12Y401/01076 , G01N2333/235 , G01N2500/00
摘要： The present invention relates to a method for the enzymatic decarboxylation of malonic acid (propanedioic acid) derivatives catalyzed by enzymes structurally and/or functionally related to arylmalonate decarboxylase (AMDase) as isolated from microorganisms of the genus Bordetella. The present invention also relates to novel enzymes with a decarboxylase activity, useful for performing the claimed method, mutants thereof, corresponding coding sequences and expression systems, methods of preparing said novel enzymes, and screening methods for obtaining further suitable enzymes also having said decarboxylase activity.
摘要翻译： 本发明涉及由结构和/或与芳基丙二酸酯脱羧酶（AMDase）功能相关的酶催化的由在博德特氏菌属微生物分离的酶催化的丙二酸（丙二酸）衍生物的酶脱羧的方法。 本发明还涉及具有脱羧酶活性的新型酶，可用于实施要求保护的方法，其突变体，相应的编码序列和表达系统，制备所述新型酶的方法，以及筛选获得还具有所述脱羧酶活性的合适酶的筛选方法 。
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公开(公告)号：US20120270280A1
公开(公告)日：2012-10-25
申请号：US13496086
申请日：2010-09-15
申请人： Nina Schneider , Bernhard Hauer , Klaus Ditrich , Maeve O'Neil , Nick Turner
发明人： Nina Schneider , Bernhard Hauer , Klaus Ditrich , Maeve O'Neil , Nick Turner
摘要： The present invention relates to a process for the biocatalytic, enantioselective production of a β-amino acid pre-cursor from an optionally substituted dihydrouracil using a hydantoinase and/or a dihydropyrimidinase, a process for producing a β-amino acid from said precursor, a hydantoinase and its use in said process for the biocatalytic production of a β-amino acid pre-cursor or a β-amino acid, and a method for obtaining said hydantoinase.
摘要翻译： 本发明涉及使用乙内酰脲酶和/或二氢嘧啶苷从任选取代的二氢尿嘧啶生物催化，对映选择性生产“氨基酸”前体的方法，一种从所述前体制备“氨基酸”的方法 ，乙内酰脲及其用于生物催化生产氨基酸前体或氨基酸的所述方法中的用途以及获得所述乙内酰脲酶的方法。
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公开(公告)号：US20120123155A1
公开(公告)日：2012-05-17
申请号：US13387140
申请日：2010-07-27
申请人： Bernhard Hauer , Nina Schneider , Dejana Drew , Klaus Ditrich , Nick Turner , Bettina M. Nestl
发明人： Bernhard Hauer , Nina Schneider , Dejana Drew , Klaus Ditrich , Nick Turner , Bettina M. Nestl
IPC分类号： C07C229/36 , C12P13/22
CPC分类号： C12P13/001 , C07C211/27
摘要： The present invention relates to a method for the enzymatic hydroamination of C—C double bonds catalyzed by enzymes structurally and/or functionally related to phenylalanine ammonia lyase (PAL) isolated from microorganisms of Petroselinum crispum, Rhodoturula glutinis and/or functional active derivatives thereof.
摘要翻译： 本发明涉及一种通过酶结构和/或功能性相关的苯丙氨酸氨裂解酶（PAL）分离的酶的催化C-C双键的方法，所述苯丙氨酸氨裂解酶（PAL）从石斛兰，红酵母和/或其功能活性衍生物的微生物分离。
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