公开(公告)号：US12203062B2

公开(公告)日：2025-01-21

申请号：US16760087

申请日：2018-11-01

Applicant: OSAKA UNIVERSITY ,  NTN CORPORATION

Inventor： Mitsuru Akashi ,  Takami Akagi ,  Masaki Egami ,  Akihiro Yamanaka ,  Haruka Nakamura ,  Toukokuryuu Ai

IPC: C12N5/00 ,  B01L3/00 ,  C12M1/00 ,  C12M1/12 ,  C12M1/26 ,  C12M1/32

Abstract: The present invention is to provide a cell chip and a three-dimensional tissue chip and a production method therefor such that even when a highly viscous cell-containing solution is a material, the highly safe and reproducible cell chip or three-dimensional tissue chip with a desired application volume can be produced within a short time and in a large quantity so as to have a desired cell density and exhibit high cell viability.

公开(公告)号：US20210284943A1

公开(公告)日：2021-09-16

申请号：US16328370

申请日：2017-08-18

Applicant: Osaka University ,  Toray Industries, Inc.

Inventor： Mitsuru Akashi ,  Yoshinori Kakizawa

IPC: C12M1/12 ,  C12M1/00 ,  C12M1/34 ,  C12N11/04 ,  C12N11/082 ,  C12N11/096 ,  C12N11/10 ,  C12N5/00 ,  B33Y70/10

Abstract: The present invention is to improve dynamic characteristics while maintaining temperature responsiveness in a cell culture support containing hydroxyalkyl chitosan, and to provide a cell culture support having temperature responsiveness, which contains temperature-responsive hydroxyalkyl chitosan and a water-soluble polymer selected from polyethylene glycol, derivatives thereof, hyaluronic acids, alginic acids and salts thereof.

公开(公告)号：US20160251626A1

公开(公告)日：2016-09-01

申请号：US15028204

申请日：2014-10-09

Applicant: OSAKA UNIVERSITY ,  PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY

Inventor： Mitsuru Akashi ,  Michiya Matsusaki ,  Yoshihiro Ishikawa ,  Utako Yokoyama

IPC: C12N5/077

CPC classification number: C12N5/0661 ,  C12N2513/00 ,  C12N2533/52 ,  C12N2533/54

Abstract: Provided are an elastic three-dimensional tissue and a method by which the tissue can be produced. The elastic three-dimensional tissue includes smooth muscle cells and an extracellular matrix component, with the smooth muscle cells being layered with the extracellular matrix component interposed therebetween. Furthermore, the production method for a three-dimensional tissue includes layering smooth muscle cells with an extracellular matrix component interposed therebetween, wherein the smooth muscle cells are those directed towards a differentiated type from an undifferentiated type.

Abstract translation: 提供了一种弹性三维组织和可以产生组织的方法。 弹性三维组织包括平滑肌细胞和细胞外基质组分，其中平滑肌细胞与介于其间的细胞外基质成分分层。 此外，用于三维组织的制备方法包括将平滑肌细胞分层插入其中的细胞外基质成分，其中平滑肌细胞是指向未分化型的分化型细胞。

公开(公告)号：US09308519B2

公开(公告)日：2016-04-12

申请号：US14646511

申请日：2013-11-19

Applicant: DAIKIN INDUSTRIES, LTD. ,  OSAKA UNIVERSITY

Inventor： Kenji Adachi ,  Masaki Kuramitsu ,  Mitsuru Akashi ,  Toshiyuki Kida

IPC: B01D15/38 ,  B01J20/26 ,  B01D15/08 ,  B01D15/26 ,  B01J20/28 ,  C07C51/50 ,  B01J20/34 ,  B01J20/32 ,  C02F1/28 ,  C08B37/16 ,  C08L5/16 ,  C02F101/36

CPC classification number: B01J20/265 ,  B01D15/08 ,  B01D15/265 ,  B01J20/261 ,  B01J20/28016 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3425 ,  B01J20/3475 ,  C02F1/285 ,  C02F1/288 ,  C02F2101/36 ,  C02F2303/16 ,  C07C51/50 ,  C08B37/0012 ,  C08B37/0015 ,  C08L5/16

Abstract: The present invention is to provide a material that is capable of selectively adsorbing organic fluoro-compounds such as perfluorooctane sulfonic acid, allows the adsorbed organic fluoro-compounds to be recovered, and is reusable as an adsorbent, specifically to provide a polymer in which cyclodextrin is supported on the surface of a water-insoluble polymer, and an adsorbent containing the same, and a method of use of the same as a selective adsorbent of, in particular, an organic fluoro-compound.

Abstract translation: 本发明提供能够选择性吸附全氟辛烷磺酸等有机氟化合物的材料，能够吸收吸附的有机氟化合物，并且作为吸附剂可以重复使用，特别是提供其中环糊精 负载在水不溶性聚合物的表面上，和含有它的吸附剂，以及使用它们作为特别是有机氟化合物的选择性吸附剂的方法。

公开(公告)号：US10799615B2

公开(公告)日：2020-10-13

申请号：US15533724

申请日：2015-12-07

Applicant: OSAKA UNIVERSITY ,  TOHOKU UNIVERSITY

Inventor： Mitsuru Akashi ,  Michiya Matsusaki ,  Michiaki Unno ,  Naoaki Sakata ,  Gumpei Yoshimatsu

IPC: A61L27/38 ,  A61K35/44 ,  A61K35/39 ,  A61L27/36 ,  C12N5/071 ,  A61L27/60 ,  A61K35/28 ,  A61K35/36 ,  C12N5/00

Abstract: An artificial tissue containing pancreatic islet cells, fibroblasts and/or cells capable of differentiating, extracellular matrix, and vascular endothelial cells, in which the fibroblasts and/or the cells capable of differentiating, the extracellular matrix, and the vascular endothelial cells constitute a three-dimensional tissue structure in which a vascular network structure has been formed, and in which the three-dimensional tissue structure contains pancreatic islets constituted by aggregating ten or more of the pancreatic islet cells.

公开(公告)号：US20150250925A1

公开(公告)日：2015-09-10

申请号：US14426013

申请日：2013-09-04

Applicant: OSAKA UNIVERSITY ,  BioMedical Technology HYBRID Co., Ltd. ,  NATIONAL UNIVERSITY CORPORATION EHIME UNIVERSITY

Inventor： Mitsuru Akashi ,  Michiya Matsusaki ,  Masaaki Nawano ,  Koji Hashimoto ,  Yuji Shirakata

IPC: A61L27/60 ,  C12N5/071

CPC classification number: A61L27/60 ,  A61F2210/0076 ,  A61L27/3633 ,  A61L27/383 ,  A61L2430/34 ,  C12N5/0639 ,  C12N5/0698 ,  C12N2501/052 ,  C12N2502/09 ,  C12N2502/1121 ,  C12N2533/54

Abstract: Provided is a novel method capable of manufacturing an artificial skin model including dendritic cells. A method for manufacturing an artificial skin model includes the following: forming a dermal tissue layer by culturing coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component, so that the coated cells are layered; forming a basal layer including type IV collagen on the dermal tissue layer by bringing type IV collagen into contact with the dermal tissue layer; and forming an epidermal layer by arranging epidermal cells on the basal layer. At least one of the dermal tissue layer and the epidermal layer includes dendritic cells.

Abstract translation: 提供了能够制造包括树突细胞的人造皮肤模型的新方法。 制造人造皮肤模型的方法包括：通过培养其中细胞表面涂覆有包含细胞外基质组分的涂膜的涂覆细胞，形成真皮组织层，使得涂覆的细胞层叠; 通过使IV型胶原与真皮组织层接触，在真皮组织层上形成包括IV型胶原蛋白的基底层; 并通过在基底层上布置表皮细胞形成表皮层。 皮肤组织层和表皮层中的至少一个包括树突状细胞。

公开(公告)号：US20150247118A1

公开(公告)日：2015-09-03

申请号：US14427536

申请日：2013-09-12

Applicant: OSAKA UNIVERSITY ,  MITSUBISHI PAPER MILLS LIMITED

Inventor： Mitsuru Akashi ,  Michiya Matsusaki ,  Atsushi Matsuzawa

IPC: C12N5/00

CPC classification number: C12N5/0062 ,  C12N5/0671 ,  C12N2533/52 ,  C12N2533/90

Abstract: Provided is a novel method for producing a three-dimensional cell culture construct. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; culturing the seeded coated cells in a culture medium; and using at least a portion of the culture medium continuously for 5 days or more. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; and culturing the seeded coated cells in a culture medium. The culture process of the coated cells includes culturing the coated cells in 70 μL or more of the culture medium per 1×104 cells.

Abstract translation: 提供了一种用于生产三维细胞培养构建体的新方法。 包含至少两层以上的层叠单元层的三维细胞培养构建体的制造方法包括以下步骤：将含有细胞外基质成分的涂膜对细胞表面进行接种的涂覆细胞， 在培养基中培养接种的包被细胞; 并将培养基的至少一部分连续使用5天以上。 包含至少两层以上的层叠单元层的三维细胞培养构建体的制造方法包括以下步骤：将含有细胞外基质成分的涂膜对细胞表面进行接种的涂覆细胞， 并在培养基中培养接种的包被的细胞。 包被细胞的培养过程包括每1×10 4个细胞培养70μL以上的培养基。