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公开(公告)号：US12173263B2
公开(公告)日：2024-12-24
申请号：US15105388
申请日：2014-12-19
Applicant: President and Fellows of Harvard College , Jose Fernandez-Alcon , Norman Wen , Richard Novak
Inventor： Jose Fernandez-Alcon , Norman Wen , Richard Novak , Donald E. Ingber , Geraldine A. Hamilton , Christopher Hinojosa , Karel Domansky , Daniel Levner , Guy Thompson, II , Kambez Hajipouran Benam , Remi Villenave , Thomas Umundum , Alfred Paris , Georg Bauer
IPC: C12M3/06 , B01D61/18 , B01D63/08 , B01D67/00 , B01L3/00 , C12M1/00 , C12M1/12 , C12M1/34 , C12M1/42 , C12M3/00
Abstract: An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.
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公开(公告)号：US11807871B2
公开(公告)日：2023-11-07
申请号：US15733716
申请日：2019-04-02
Applicant: President and Fellows of Harvard College
Inventor： Richard Novak , Sasan Jalili-Firoozinezhad , Francesca S. Gazzaniga , Elizabeth L. Calamari , Diogo M. Camacho , Bret A. Nestor , Cicely Fadel , Michael L. Cronce , Dennis L. Kasper , Donald E. Ingber , Amir Bein
CPC classification number: C12N5/0679 , C12M21/08 , C12M23/16 , C12N1/20
Abstract: A microfluidic device is directed to sustaining a complex microbial community in direct and indirect contact with living human intestinal cells in vitro. The device includes a first microchannel having cultured cells of a human intestinal epithelium and microbiota, the first microchannel further having a first level of oxygen. The device further includes a second microchannel having cultured cells of a vascular endothelium, the second microchannel further having a second level of oxygen. The device also includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane being composed of an oxygen-permeable material or further having pores via which oxygen flows between the first microchannel and the second microchannel to form a physiologically-relevant oxygen gradient.
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公开(公告)号：US11325119B2
公开(公告)日：2022-05-10
申请号：US16494230
申请日：2018-03-14
Applicant: President and Fellows of Harvard College
Inventor： Carlos Francisco NG Pitti , Ulri Nicole Lee , Richard Novak , Olivier Yves Frederic Henry , Remco Van Erp , Donald E. Ingber
IPC: B01L3/00 , G01N27/08 , G01N33/487
Abstract: A fluidic device includes a fluidic layer, a capture material, and an electronics layer, the fluidic layer includes a main channel and a pair of sample channels fluidly coupled to the main channel. The pair of sample channels is configured to receive and introduce a sample material into the device. The sample material includes an analyte. The capture material is positioned in a portion of the main channel that is spaced from the pair of sample channels. The capture material has a three-dimensional matrix of receptors therein configured to bond with the analyte. The capture material has a length that is associated with a dynamic range of the fluidic device and a cross-sectional area that is associated with a sensitivity of the fluidic device. The electronics layer includes electrodes configured to measure an electrical resistance through a portion of the capture material.
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公开(公告)号：US20210079356A1
公开(公告)日：2021-03-18
申请号：US15733716
申请日：2019-04-02
Applicant: President and Fellows of Harvard College
Inventor： Richard Novak , Sasan Jalili-Firoozinezhad , Francesca S. Gazzaniga , Elizabeth L. Calamari , Diogo M. Camacho , Bret A. Nestor , Cicely Fadel , Michael L. Cronce , Dennis L. Kasper , Donald E. Ingber , Amir Bein
Abstract: A microfluidic device is directed to sustaining a complex microbial community in direct and indirect contact with living human intestinal cells in vitro. The device includes a first microchannel having cultured cells of a human intestinal epithelium and microbiota, the first microchannel further having a first level of oxygen. The device further includes a second microchannel having cultured cells of a vascular endothelium, the second microchannel further having a second level of oxygen. The device also includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane being composed of an oxygen-permeable material or further having pores via which oxygen flows between the first microchannel and the second microchannel to form a physiologically-relevant oxygen gradient.
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公开(公告)号：US20180346859A1
公开(公告)日：2018-12-06
申请号：US15781078
申请日：2016-12-02
Applicant: President and Fellows of Harvard College
Inventor： Antonio Varone , Norman Wen , Daniel Levner , Richard Novak , Lori McPartlin , Donald E. Ingber , Youngjae Choe , Lian Leng , Justin K. Nguyen
Abstract: A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.
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Patent Agency Ranking
公开(公告)号：US20180071690A1
公开(公告)日：2018-03-15
申请号：US15558191
申请日：2016-03-16
Applicant: President and Fellows of Harvard College
Inventor： James Coon , Tiama Hamkins-Indik , Donald E. Ingber , Miles Ingram , Daniel Levner , Richard Novak , Jefferson Puerta , Daniel E. Shea , Josiah Sliz , Norman Wen
IPC: B01D67/00 , B01D71/70 , B29C41/38 , B29C33/42 , B29C33/68 , B29C39/00 , B29C39/26 , B29C41/12 , C12M1/12
CPC classification number: B01D67/0034 , B01D71/70 , B01L3/502707 , B01L2300/123 , B01L2400/086 , B29C33/424 , B29C33/68 , B29C39/006 , B29C39/26 , B29C41/12 , B29C41/36 , B29C41/38 , B29C2043/025 , B29L2031/755 , B29L2031/756 , C12M25/02
Abstract: A method for micro-molding a polymeric membrane and including pouring a predetermined volume of curable polymer unto a micro-fabricated mold having a post array with pillars, and overlaying the polymer with a support substrate. A spacer, such as a rubber spacer, is placed in contact with the support substrate and a force is applied to an exposed side of the spacer to compress the support substrate and the polymer together. While applying the force, the polymer is cured on the mold for a predetermined time period and at a predetermined temperature to form a polymeric membrane having a pore array with a plurality of pores corresponding to the plurality of pillars of the post array. The polymeric membrane is removed from the support substrate.
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公开(公告)号：US20240002808A1
公开(公告)日：2024-01-04
申请号：US18226184
申请日：2023-07-25
Applicant: President and Fellows of Harvard College
Inventor： Richard Novak , Sasan Jalili-Firoozinezhad , Francesca S. Gazzaniga , Elizabeth Calamari , Diogo M. Camacho , Bret Andrew Nestor , Cicely Fadel , Michael J. Cronce , Dennis Kasper , Donald E. Ingber , Amir Bein
CPC classification number: C12N5/0679 , C12N1/20 , C12M23/16 , C12M21/08
Abstract: A microfluidic device is directed to sustaining a complex microbial community in direct and indirect contact with living human intestinal cells in vitro. The device includes a first microchannel having cultured cells of a human intestinal epithelium and microbiota, the first microchannel further having a first level of oxygen. The device further includes a second microchannel having cultured cells of a vascular endothelium, the second microchannel further having a second level of oxygen. The device also includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane being composed of an oxygen-permeable material or further having pores via which oxygen flows between the first microchannel and the second microchannel to form a physiologically-relevant oxygen gradient.
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公开(公告)号：US11654399B2
公开(公告)日：2023-05-23
申请号：US15558191
申请日：2016-03-16
Applicant: President and Fellows of Harvard College
Inventor： David James Coon , Tiama Hamkins-Indik , Donald E. Ingber , Miles Ingram , Daniel Levner , Richard Novak , Jefferson Puerta , Daniel E. Shea , Josiah Sliz , Norman Wen
IPC: B01D67/00 , B29C41/38 , B29C33/42 , B29C33/68 , B29C39/00 , B29C39/26 , B29C41/12 , B01L3/00 , B29C41/36 , B01D71/70 , C12M1/12 , B29L31/00 , B29C43/02
CPC classification number: B01D67/0034 , B01D71/70 , B01L3/502707 , B29C33/424 , B29C33/68 , B29C39/006 , B29C39/26 , B29C41/12 , B29C41/36 , B29C41/38 , C12M25/02 , B01L2300/123 , B01L2400/086 , B29C2043/025 , B29L2031/755 , B29L2031/756
Abstract: A method for micro-molding a polymeric membrane and including pouring a predetermined volume of curable polymer unto a micro-fabricated mold having a post array with pillars, and overlaying the polymer with a support substrate. A spacer, such as a rubber spacer, is placed in contact with the support substrate and a force is applied to an exposed side of the spacer to compress the support substrate and the polymer together. While applying the force, the polymer is cured on the mold for a predetermined time period and at a predetermined temperature to form a polymeric membrane having a pore array with a plurality of pores corresponding to the plurality of pillars of the post array. The polymeric membrane is removed from the support substrate.
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9.发明申请公开(公告)号：US20200263118A1
公开(公告)日：2020-08-20
申请号：US15781080
申请日：2016-12-02
Applicant: President and Fellows of Harvard College
Inventor： Kambez Hajipouran Benam , Richard Novak , Donald E. Ingber
Abstract: A device is directed to simulating a function of a tissue, and includes a first structure defining a first chamber, a second structure defining a second chamber, and a porous membrane located at an interface region between the first chamber and the second chamber. The membrane has a first side facing toward the first chamber and a second side facing toward the second chamber, the membrane separating the first chamber from the second chamber. The first side includes a fluid-permeable, stimulus-responsive polymer gel thereon, the second side including at least one layer of cells adhered thereon.
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公开(公告)号：US20170327781A1
公开(公告)日：2017-11-16
申请号：US15601430
申请日：2017-05-22
Applicant: President and Fellows of Harvard College
Inventor： Jose Fernandez-Alcon , Norman Wen , Richard Novak , Donald E. Ingber , Geraldine A. Hamilton , Christopher Hinojosa , Karel Domansky , Daniel Levner , Guy Thompson , Kambez Hajipouran Benam , Remi Villenave , Thomas Umundum , Alfred Paris , Georg Bauer
CPC classification number: C12M23/16 , C12M21/08 , C12M23/26 , C12M25/02 , C12M35/04 , C12M41/00 , C12M41/46
Abstract: An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.
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