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1.发明授权Cloning and sequencing of the gene which codes for a new pilinic subunit of Bordetella pertussis 失效克隆和测序法，用于BORDETELLA PERTUSSIS的新PILINIC亚纲
公开(公告)号：US5059537A
公开(公告)日：1991-10-22
申请号：US288169
申请日：1988-12-22
申请人： Paola Pedroni , Barbara Riboli , Francesca De Ferra , Guido Grandi , Salvatore Toma , Beatrice Arico' , Rino Rappuoli
发明人： Paola Pedroni , Barbara Riboli , Francesca De Ferra , Guido Grandi , Salvatore Toma , Beatrice Arico' , Rino Rappuoli
IPC分类号： C12N1/20 , A61K38/00 , A61K39/00 , A61P31/04 , A61P37/04 , C07K14/00 , C07K14/195 , C07K14/235 , C07K14/41 , C12N1/19 , C12N1/21 , C12P21/02 , C12R1/01 , C12R1/19
CPC分类号： C07K14/235 , A61K39/00
摘要： The cloning and sequencing of the gene which codes for a new pilinic subunit of Bordetella pertussis are described. The aminoacid sequence of the mature subunit, deduced from its necleotide sequence, is similar but not identical to that of the known pilins 2, 3, and 6. Polypeptides having the aminoacid sequence of the mature pilinic subunit or of regions thereof are particularly useful for the development of synthetic acellular vaccines against pertussis.
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2.发明授权Cloning and sequencing of the gene which codes for a new pilinic subunit of bordella pertussis 失效编码百日咳杆菌新型番茄亚基的基因的克隆和测序
公开(公告)号：US5622706A
公开(公告)日：1997-04-22
申请号：US334425
申请日：1994-11-04
申请人： Paola Pedroni , Barbara Riboli , Francesca De Ferra , Guido Grandi , Salvatore Toma , Beatrice Arico , Rino Rappuoli
发明人： Paola Pedroni , Barbara Riboli , Francesca De Ferra , Guido Grandi , Salvatore Toma , Beatrice Arico , Rino Rappuoli
IPC分类号： C12N1/20 , A61K38/00 , A61K39/00 , A61P31/04 , A61P37/04 , C07K14/00 , C07K14/195 , C07K14/235 , C07K14/41 , C12N1/19 , C12N1/21 , C12P21/02 , C12R1/01 , C12R1/19 , A61K39/02 , A61K39/10 , C12N15/00
CPC分类号： C07K14/235 , A61K39/00
摘要： The cloning and sequencing of the gene which codes for a new pilinic subunit of Bordetella pertussis are described. The aminoacid sequence of the mature subunit, deduced from its necleotide sequence, is similar but not identical to that of the known pilins 2, 3 and 6. Polypeptides having the aminoacid sequence of the mature pilinic subunit or of regions thereof are particularly useful for the development of synthetic acellular vaccines against pertussis.
摘要翻译： 描述了编码百日咳博德特氏菌新型番茄亚基的基因的克隆和测序。 从其核苷酸序列推导的成熟亚单位的氨基酸序列与已知的pilins 2,3和6相似但不相同。具有成熟皮质亚单位或其区域的氨基酸序列的多肽特别可用于 开发针对百日咳的合成无细胞疫苗。
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3.发明授权
公开(公告)号：US5252478A
公开(公告)日：1993-10-12
申请号：US658908
申请日：1991-02-22
申请人： Immaculada Margarit y Ros , Susanna Campagnoli , Roberto Gianna , Salvatore Toma , Guido Grandi
发明人： Immaculada Margarit y Ros , Susanna Campagnoli , Roberto Gianna , Salvatore Toma , Guido Grandi
摘要： The mutants of neutral protease (NP) described can retain their enzymatic activities at temperatures at which wild-type neutral protease becomes inactive, the mutants being characterised in that the Gly 189 and/or Gly 147 residues of the aminoacid sequence of NP are replaced by different residues selected from the natural aminoacids.Mutagenised genes of neutral protease which code for the mutants, recombinant plasmids containing the genes, and strains of Bacillus transformed by the plasmids are also described. The mutants of neutral protease having the characteristics described above are particularly useful in the food sector.
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4.发明授权Cloning vector, molecules of recombinant DNA, bacillus subtilis strains transformed with the said molecules and methods for the expression of heterologous genes and the production and secretion of proteins coded by the said genes 失效克隆载体，重组DNA分子，用所述分子转化的枯草芽孢杆菌菌株和用于表达异源基因的方法以及由所述基因编码的蛋白质的产生和分泌
公开(公告)号：US5021340A
公开(公告)日：1991-06-04
申请号：US395173
申请日：1989-08-17
申请人： Salvatore Toma , Marina Del Bue , Antonio Mele , Guido Grandi
发明人： Salvatore Toma , Marina Del Bue , Antonio Mele , Guido Grandi
IPC分类号： C12N15/09 , C07K14/585 , C12N1/20 , C12N1/21 , C12N9/54 , C12N15/00 , C12N15/75 , C12P21/02 , C12R1/125
CPC分类号： C07K14/585 , C12N15/75 , C12N9/54 , C07K2319/00 , C07K2319/02 , C07K2319/036 , C07K2319/61 , C07K2319/75 , Y10S435/839
摘要： A description is given of a cloning vector useful for the expression and secretion of heterologous proteins in Bacillus subtilis, molecules of recombinant DNA formed by the said cloning vector, expediently cut at a restriction site located within the neutral protease gene, and by the sequence of heterologous DNA which codes for the protein of interest, Bacillus subtilis cells transformed with the said molecules and capable of expressing the sequence of heterologous DNA and of producing and secreting the protein coded by the said sequence of heterologous DNA in high yields.The proteins obtained in this manner are useful in the fields of pharmaceuticals and foods, or as compounds for the production of substances which are normally obtained by chemical synthesis.
摘要翻译： 给出了用于在枯草芽孢杆菌中表达和分泌异源蛋白质的克隆载体的描述，由所述克隆载体形成的重组DNA的分子，有利地在位于中性蛋白酶基因内的限制性位点切割，以及序列 编码感兴趣的蛋白质的异源DNA，用所述分子转化的能够表达异源DNA序列的枯草芽孢杆菌细胞，并以高产率产生和分泌由所述异源DNA序列编码的蛋白质。 以这种方式获得的蛋白质可用于药物和食品领域，或用作生产通常通过化学合成获得的物质的化合物。
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公开(公告)号：US20130028876A1
公开(公告)日：2013-01-31
申请号：US13601174
申请日：2012-08-31
CPC分类号： C12N9/1211 , A61K48/0058
摘要： A polynucleotide comprising a nucleotide sequence encoding a thymidine kinase wherein at least one of the nucleotides corresponding to the splice donor site nucleotides is replaced by another nucleotide and wherein the nucleotides of the splice acceptor sites are not altered.
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公开(公告)号：US20070166291A1
公开(公告)日：2007-07-19
申请号：US11568604
申请日：2005-05-05
申请人： Claudia Benati , Robert Sciarretta , Simona La Seta Catamancio , Marina Radrizzani , Cecilia Sendresen , Salvatore Toma
发明人： Claudia Benati , Robert Sciarretta , Simona La Seta Catamancio , Marina Radrizzani , Cecilia Sendresen , Salvatore Toma
IPC分类号： A61K48/00 , C12N15/867
CPC分类号： C12N15/86 , C12N2510/00 , C12N2740/13043
摘要： A method for transduction of cells with a genetic construct and selection of genetically modified cells, the method comprising performing the transduction and selection in a closed system.
摘要翻译： 一种用遗传构建体转染细胞的方法和选择遗传修饰的细胞，该方法包括在封闭系统中进行转导和选择。
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7.发明授权Tankyrase homolog protein (THP), nucleic acids, and methods related to the same 失效巯基酶同源蛋白（THP），核酸和与之相关的方法公开(公告)号：US06455290B1
公开(公告)日：2002-09-24
申请号：US09350982
申请日：1999-07-09
IPC分类号： C12N912
CPC分类号： C12N9/1077 , A61K38/00 , C12N2799/021 , C12N2799/026 , C12Y204/0203
摘要： The present invention provides a human polypeptide homolog of human tankyrase protein (THP) and polynucleotides which identify and encode THP. In addition, the invention provides expression vectors, host cells and methods for its production. The invention also provides methods for the identification of THP agonists/antagonists, useful for the treatment of human diseases, such as human cancer and age related diseases.
摘要翻译： 本发明提供人类巯基酶蛋白（THP）的人多肽同源物和识别和编码THP的多核苷酸。 此外，本发明提供表达载体，宿主细胞及其生产方法。 本发明还提供了用于鉴定可用于治疗人类疾病如人类癌症和与年龄有关的疾病的THP激动剂/拮抗剂的方法。
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公开(公告)号：US08642311B2
公开(公告)日：2014-02-04
申请号：US13601174
申请日：2012-08-31
IPC分类号： C12N9/12
CPC分类号： C12N9/1211 , A61K48/0058
摘要： A polynucleotide comprising a nucleotide sequence encoding a thymidine kinase wherein at least one of the nucleotides corresponding to the splice donor site nucleotides is replaced by another nucleotide and wherein the nucleotides of the splice acceptor sites are not altered.
摘要翻译： 包含编码胸苷激酶的核苷酸序列的多核苷酸，其中对应于剪接供体位点核苷酸的至少一个核苷酸被另一核苷酸替代，并且其中所述剪接受体位点的核苷酸不被改变。
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公开(公告)号：US08357788B2
公开(公告)日：2013-01-22
申请号：US11629962
申请日：2005-06-17
CPC分类号： C12N9/1211 , A61K48/0058
摘要： A polynucleotide comprising a nucleotide sequence encoding a thymidine kinase wherein at least one of the nucleotides corresponding to the splice donor site nucleotides is replaced by another nucleotide and wherein the nucleotides of the splice acceptor sites are not altered.
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公开(公告)号：US20090130069A1
公开(公告)日：2009-05-21
申请号：US11629962
申请日：2005-06-17
IPC分类号： A61K35/12 , C12N15/11 , C12N15/00 , C12N5/06 , A61K31/7088
CPC分类号： C12N9/1211 , A61K48/0058
摘要： A polynucleotide comprising a nucleotide sequence encoding a thymidine kinase wherein at least one of the nucleotides corresponding to the splice donor site nucleotides is replaced by another nucleotide and wherein the nucleotides of the splice acceptor sites are not altered.
摘要翻译： 包含编码胸苷激酶的核苷酸序列的多核苷酸，其中对应于剪接供体位点核苷酸的至少一个核苷酸被另一个核苷酸替代，并且其中所述剪接受体位点的核苷酸不被改变。
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