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公开(公告)号：US09410210B2
公开(公告)日：2016-08-09
申请号：US13701935
申请日：2011-06-06
申请人： Patrick A. Mach , Raj Rajagopal , Wensheng Xia , Jinsheng Zhou , Chunmei Guo , Guoping Mao
发明人： Patrick A. Mach , Raj Rajagopal , Wensheng Xia , Jinsheng Zhou , Chunmei Guo
CPC分类号： C12Q1/04 , B01D63/00 , B01D69/02 , B01D69/10 , B01D2323/39 , B01D2325/022 , B01D2325/12 , B01J20/267 , B01J20/28047 , B01J20/2805 , C12Q1/24 , C12Q1/689 , G01N33/68
摘要： A method of filtering a liquid sample that includes passing a sample comprising at least one biological organism through a filter membrane at a passive water volume flux of at least 10 L/m2·h·psi, wherein the filter membrane comprises a Bubble Point pore size of no more than 1.0 μm, thereby retaining at least one biological organism on the surface of the membrane; and detecting the at least one biological organism retained on the surface of the filter membrane.
摘要翻译： 一种过滤液体样品的方法，其包括使包含至少一种生物体的样品通过过滤膜以至少10L / m2·h·psi的被动水体积通量通过，其中所述滤膜包括泡点孔径 不超过1.0μm，从而在膜的表面上保留至少一种生物有机体; 以及检测保留在所述过滤膜表面上的所述至少一个生物体。
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公开(公告)号：US20130130270A1
公开(公告)日：2013-05-23
申请号：US13700910
申请日：2011-06-06
申请人： Raj Rajagopal , Wensheng Xia , Jinsheng Zhou , James E. Aysta , Guoping Mao
发明人： Raj Rajagopal , Wensheng Xia , Jinsheng Zhou , James E. Aysta , Chunmei Guo
IPC分类号： C12Q1/24
CPC分类号： C12Q1/24 , C12Q1/04 , G01N33/56911 , G01N2001/4088
摘要： A method of filtering a liquid sample that includes passing a sample comprising at least one biological organism through a filter membrane at a water volume flux of at least 100 L/m2.h.psi, where the filter membrane comprises a Bubble Point pore size of no more than 1.0 μm and where at least one biological organism is retained on the surface of the membrane. The method further includes detecting the at least one biological organism retained on the surface of the filter membrane.
摘要翻译： 一种过滤液体样品的方法，其包括使包含至少一种生物体的样品通过过滤膜以至少100L / m2.h.psi的水体积通量通过，其中过滤膜包括泡沫点孔径 不超过1.0μm，并且其中至少一个生物有机体保留在膜的表面上。 该方法还包括检测保留在过滤膜表面上的至少一个生物体。
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公开(公告)号：US09243279B2
公开(公告)日：2016-01-26
申请号：US13989922
申请日：2011-12-22
摘要： A latent effervescent body comprising a selective agent is disclosed. A method of using the latent effervescent body in a method to selectively enrich a target microorganism is also disclosed. The method comprises providing a sample, a culture medium, and the latent effervescent body. The method further comprises contacting the sample, the culture medium, and the latent effervescent body under conditions to facilitate growth of the target microorganism. The method further comprises releasing the selective agent from the latent effervescent body. Optionally, the method includes detecting a microorganism.
摘要翻译： 公开了包含选择剂的潜伏泡腾体。 还公开了在选择性富集目标微生物的方法中使用潜在泡腾体的方法。 该方法包括提供样品，培养基和潜在泡腾体。 该方法还包括使样品，培养基和潜在泡腾体在有助于靶微生物生长的条件下接触。 该方法还包括从潜在泡腾体释放选择剂。 任选地，该方法包括检测微生物。
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公开(公告)号：US20130266955A1
公开(公告)日：2013-10-10
申请号：US13989922
申请日：2011-12-22
IPC分类号： C12Q1/04
摘要： A latent effervescent body comprising a selective agent is disclosed. A method of using the latent effervescent body in a method to selectively enrich a target microorganism is also disclosed. The method comprises providing a sample, a culture medium, and the latent effervescent body. The method further comprises contacting the sample, the culture medium, and the latent effervescent body under conditions to facilitate growth of the target microorganism. The method further comprises releasing the selective agent from the latent effervescent body. Optionally, the method includes detecting a microorganism.
摘要翻译： 公开了包含选择剂的潜伏泡腾体。 还公开了在选择性富集目标微生物的方法中使用潜在泡腾体的方法。 该方法包括提供样品，培养基和潜在泡腾体。 该方法还包括使样品，培养基和潜在泡腾体在有助于靶微生物生长的条件下接触。 该方法还包括从潜在泡腾体释放选择剂。 任选地，该方法包括检测微生物。
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公开(公告)号：US20100151469A1
公开(公告)日：2010-06-17
申请号：US12597394
申请日：2008-04-25
IPC分类号： C12Q1/68 , C12N9/00 , C12N9/48 , C12N9/36 , C12N9/50 , C12N9/42 , C12N1/06 , C40B40/10 , C12M1/00
CPC分类号： C12N15/1006
摘要： Stable compositions comprising a nucleic acid binding support material comprising a substrate with functional groups attached to the substrate; a lysing enzyme; a water dispersible matrix material; and a saccharide, methods of using the compositions, and products and devices which include the compositions are disclosed.
摘要翻译： 包含核酸结合支持材料的稳定组合物，所述核酸结合载体材料包含具有连接至所述底物的官能团的底物; 裂解酶; 水分散基质材料; 和糖，公开了使用组合物的方法，以及包括该组合物的产品和装置。
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公开(公告)号：US07122383B2
公开(公告)日：2006-10-17
申请号：US10098387
申请日：2002-03-18
申请人： Robert M. Jones , Sriram Kumaraswamy , Liangde Lu , Frauke Rininsland , Kevin Ley , Wensheng Xia , Duncan McBranch , David G. Whitten
发明人： Robert M. Jones , Sriram Kumaraswamy , Liangde Lu , Frauke Rininsland , Kevin Ley , Wensheng Xia , Duncan McBranch , David G. Whitten
IPC分类号： G01N33/543
CPC分类号： B82Y20/00 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C12Q1/6834 , G01N33/533 , G01N33/542 , G01N33/54393 , G01N33/582 , Y10T428/2991 , Y10T436/143333 , C12Q2563/107
摘要： A chemical composition including a fluorescent polymer and a receptor that is specific for both a target biological agent and a chemical moiety including (a) a recognition element, (b) a tethering element, and (c) a property-altering element is disclosed. Both the fluorescent polymer and the receptor are co-located on a support. When the chemical moiety is bound to the receptor, the property-altering element is sufficiently close to the fluorescent polymer to alter the fluorescence emitted by the polymer. When an analyte sample is introduced, the target biological agent, if present, binds to the receptor, thereby displacing the chemical moiety from the receptor, resulting in an increase of detected fluorescence. Assays for detecting the presence of a target biological agent are also disclosed.
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公开(公告)号：US20130344488A1
公开(公告)日：2013-12-26
申请号：US13701935
申请日：2011-06-06
申请人： Guoping Mao
发明人： Patrick A. Mach , Raj Rajagopal , Wensheng Xia , Jinsheng Zhou , Chunmei Guo
CPC分类号： C12Q1/04 , B01D63/00 , B01D69/02 , B01D69/10 , B01D2323/39 , B01D2325/022 , B01D2325/12 , B01J20/267 , B01J20/28047 , B01J20/2805 , C12Q1/24 , C12Q1/689 , G01N33/68
摘要： A method of filtering a liquid sample that includes passing a sample comprising at least one biological organism through a filter membrane at a passive water volume flux of at least 10 L/m2·h·psi, wherein the filter membrane comprises a Bubble Point pore size of no more than 1.0 μm, thereby retaining at least one biological organism on the surface of the membrane; and detecting the at least one biological organism retained on the surface of the filter membrane.
摘要翻译： 一种过滤液体样品的方法，其包括使包含至少一种生物体的样品通过过滤膜以至少10L / m2·h·psi的被动水体积通量通过，其中所述滤膜包括泡点孔径 不超过1.0μm，从而在膜的表面上保留至少一种生物有机体; 以及检测保留在所述过滤膜表面上的所述至少一个生物体。
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公开(公告)号：US09255864B2
公开(公告)日：2016-02-09
申请号：US14001895
申请日：2012-03-05
CPC分类号： G01N1/30 , B01L3/502 , B01L3/5023 , B01L3/50255 , B01L2200/026 , B01L2200/028 , B01L2200/10 , B01L2300/0681 , B01L2300/069 , B01L2300/0832 , B01L2300/0861 , B01L2300/0887 , B01L2400/049 , C12N15/1017 , G01N1/34
摘要： A first apparatus (100) for processing a liquid sample is disclosed. The apparatus (100) includes a sample-receiving (120), a filtrate-receiving component (150), and an analyte-capture element (170). The apparatus (100) forms a liquid flow path through which the sample passes, thereby causing the analyte-capture element (170) to capture an analyte, if present. A method is disclosed whereby a liquid sample is passed through the first (100) apparatus and the analyte-capture element (170) is easily separated from the apparatus for further processing and detection of the analyte. A structurally-related second apparatus (200) for processing a plurality of liquid samples, and a corresponding method of use, also is disclosed.
摘要翻译： 公开了一种用于处理液体样品的第一装置（100）。 装置（100）包括样品接收（120），滤液接收组分（150）和分析物捕获元件（170）。 装置（100）形成液体流动通道，样品通过该流路，从而使得分析物捕获元件（170）捕获分析物（如果存在）。 公开了一种方法，其中液体样品通过第一（100）装置，并且分析物捕获元件（170）容易与装置分离，用于进一步处理和检测分析物。 还公开了一种用于处理多个液体样品的结构相关的第二装置（200）和相应的使用方法。
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公开(公告)号：US20130344478A1
公开(公告)日：2013-12-26
申请号：US14001895
申请日：2012-03-05
IPC分类号： G01N1/34
CPC分类号： G01N1/30 , B01L3/502 , B01L3/5023 , B01L3/50255 , B01L2200/026 , B01L2200/028 , B01L2200/10 , B01L2300/0681 , B01L2300/069 , B01L2300/0832 , B01L2300/0861 , B01L2300/0887 , B01L2400/049 , C12N15/1017 , G01N1/34
摘要： A first apparatus (100) for processing a liquid sample is disclosed. The apparatus (100) includes a sample-receiving (120), a filtrate-receiving component (150), and an analyte-capture element (170). The apparatus (100) forms a liquid flow path through which the sample passes, thereby causing the analyte-capture element (170) to capture an analyte, if present. A method is disclosed whereby a liquid sample is passed through the first (100) apparatus and the analyte-capture element (170) is easily separated from the apparatus for further processing and detection of the analyte. A structurally-related second apparatus (200) for processing a plurality of liquid samples, and a corresponding method of use, also is disclosed.
摘要翻译： 公开了一种用于处理液体样品的第一装置（100）。 装置（100）包括样品接收（120），滤液接收组分（150）和分析物捕获元件（170）。 装置（100）形成液体流路，样品通过该流路，从而使分析物捕获元件（170）捕获分析物（如果存在）。 公开了一种方法，其中液体样品通过第一（100）装置，并且分析物捕获元件（170）容易与装置分离，以进一步处理和检测分析物。 还公开了一种用于处理多个液体样品的结构相关的第二装置（200）和相应的使用方法。
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公开(公告)号：US20100129878A1
公开(公告)日：2010-05-27
申请号：US12596346
申请日：2008-04-25
申请人： Ranjani V. Parthasarathy , Hsi-Chou Liu , Wensheng Xia , Assumpta Bennaars-Eiden , Paul N. Holt , William Bedingham , Christopher R. Kokaisel , Jeffrey C. Pederson
发明人： Ranjani V. Parthasarathy , Hsi-Chou Liu , Wensheng Xia , Assumpta Bennaars-Eiden , Paul N. Holt , William Bedingham , Christopher R. Kokaisel , Jeffrey C. Pederson
IPC分类号： C12P19/34
CPC分类号： B01L3/502753 , B01L7/52 , B01L2200/0647 , B01L2300/0806 , B01L2400/0677 , C12Q1/6806 , C12Q1/6846 , C12Q2563/149 , C12Q2563/137
摘要： The present invention provides methods for amplifying nucleic acid after isolating nucleic acid from a sample using functionalized support material.
摘要翻译： 本发明提供了使用官能化载体材料从样品中分离核酸后扩增核酸的方法。
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