公开(公告)号：US20100112720A1

公开(公告)日：2010-05-06

申请号：US12448193

申请日：2007-12-10

申请人： Per Mansson ,  Bjorn Sanden

发明人： Per Mansson ,  Bjorn Sanden

IPC分类号： G01N33/22

CPC分类号： G01N33/54373

摘要： A continuously repeatable method of detecting possible presence of one or several different selected target antigen(s), such as explosives and/or narcotics, in a predetermined test volume with a flow-through analysis device, such as a Piezoelectric Quartz Crystal Microbalance device (QCM), an Ellipsometer device or a Surface Plasmon Resonance biosensor device, is described. The device is equipped with one or several fluidly connected, individually operated, flow-through testing cells, each cell containing a sensing surface, each sensing surface comprising a selected modified target antigen immobilized thereon, which modified antigen has weaker affinity than the target antigen for a selected antibody specific for the selected target antigen. One testing cell is for analyzing a target antigen by the competition mode, whereas optional other testing cells are individually selected for analysis by the competition mode or the displacement mode.

摘要翻译： 使用流通分析装置（例如压电石英晶体微量平衡装置）（例如，压电石英晶体微量平衡装置），在预定试验体积中检测可能存在一种或几种不同的选定目标抗原（例如爆炸物和/或麻醉剂）的连续可重复的方法 QCM），椭偏仪装置或表面等离子体共振生物传感器装置。 该装置配备有一个或多个流体连接的，单独操作的流通测试单元，每个单元包含感测表面，每个感测表面包含固定在其上的选定的修饰的靶抗原，所述修饰的抗原具有比目标抗原弱的亲和力 选择的针对所选靶抗原特异的抗体。 一个测试单元用于通过竞争模式分析目标抗原，而任选的其他测试单元被单独选择以通过竞争模式或位移模式进行分析。

公开(公告)号：US20060105471A1

公开(公告)日：2006-05-18

申请号：US10517322

申请日：2003-06-18

申请人： Per Mansson ,  Ann-Sofie Johansson ,  Bjorn Sanden ,  Shahin Loniakan

发明人： Per Mansson ,  Ann-Sofie Johansson ,  Bjorn Sanden ,  Shahin Loniakan

IPC分类号： G01N33/543 ,  C12M1/34

CPC分类号： G01N33/946 ,  G01N33/22 ,  G01N33/54353 ,  G01N33/553

摘要： A coated metal surface on a solid support, wherein the coating consists of a protein layer firmly attached to the metal surface, and said protein layer is coupled to linker molecules that are bound to low molecular weight antigens, wherein the linker molecules are coupled to the protein layer and are bound to the antigen via functional end groups and contain between the functional end groups an aliphatic hydrocarbon of 1, 2 or 3 carbon atoms, and wherein the antigens are optionally reversibly bound to antibodies specific for the antigens, is described. The coated metal surface on a solid support may be used in a method of detecting analyte antigens as part of an analysis device, such as a Piezoelectric Crystal Microbalance device or a Surface Plasmon Resonance biosensor, for detection in an aqueous solution of an analyte antigen with higher affinity to an antibody than the antigen of the coating by monitoring the displacement of the antibody from the coating.

摘要翻译： 在固体支持物上的涂覆的金属表面，其中所述涂层由牢固连接到所述金属表面的蛋白质层组成，并且所述蛋白质层与结合到低分子量抗原的接头分子偶联，其中所述连接分子与 蛋白质层，并且通过官能端基与抗原结合，并且在功能性端基之间含有1,2或3个碳原子的脂族烃，并且其中抗原任选地可逆地结合抗原特异性抗体。 固体支持物上的涂覆的金属表面可以用于检测作为分析装置的一部分的分析物抗原的方法，例如压电晶体微量天平装置或表面等离子体共振生物传感器，用于在分析物抗原的水溶液中检测， 通过监测来自涂层的抗体的置换，对抗体比涂层的抗原更高的亲和性。

公开(公告)号：US20060057635A1

公开(公告)日：2006-03-16

申请号：US10517320

申请日：2003-06-18

申请人： Per Mansson ,  Bo Liedberg

发明人： Per Mansson ,  Bo Liedberg

IPC分类号： G01N33/53 ,  C12M1/34

CPC分类号： B82Y30/00 ,  B82Y15/00 ,  G01N33/54353 ,  G01N2610/00 ,  Y10T428/31678

摘要： A coated metal surface on a solid support, wherein the coating consists of a self-assembled monolayer (SAM) of oligo(ethylene glycol)-terminated amide group-containing alkyl thiols firmly attached to the metal surface via the thiol-end and low molecular weight antigens bound via an amide-group to the SAM-forming OEG molecule, wherein the alkyl portion has 1-20 methylene groups, wherein the oligo(ethylene glycol) portion has 1-15 ethylene oxy units, and wherein the antigens, such as explosives and narcotics, are optionally reversibly bound to antibodies specific for the antigens, is disclosed. The coated metal surface on a solid support may be used in a method of detecting analyte antigens as part of an analysis device, such as a Piezoelectric Crystal Microbalance device or a Surface Plasmon Resonance biosensor, for detection in an aqueous solution of an analyte antigen with higher affinity to an antibody than the antigen of the coating by monitoring the displacement of the antibody from the coating.

公开(公告)号：US5375611A

公开(公告)日：1994-12-27

申请号：US8947

申请日：1993-01-26

申请人： Bengt Lindqvist ,  Per Mansson ,  Tomas Malson

发明人： Bengt Lindqvist ,  Per Mansson ,  Tomas Malson

IPC分类号： A61F9/007 ,  A61K31/715 ,  A61K31/765 ,  A61K31/77 ,  A61L27/34 ,  A61P27/02 ,  A61P27/12 ,  A61B17/00

CPC分类号： A61L27/34 ,  A61F9/00736 ,  A61L2430/16

摘要： Method for extracapsular cataract extraction with or without posterior chamber intraocular lens implantation comprising chemical modification of the posterior surface of the lens capsule, at least in the optical portion of said capsule, for preventing cell attachment and growth.

摘要翻译： 用于具有或不具有后房人工晶状体植入物的囊外白内障摘除的方法，包括至少在所述胶囊的光学部分中的用于防止细胞附着和生长的晶状体囊的后表面的化学修饰。

公开(公告)号：US20080050795A1

公开(公告)日：2008-02-28

申请号：US10580232

申请日：2004-11-19

申请人： Per Mansson ,  Daniel Berggren ,  Carl Lundberg ,  Ann-Charlotte Hellgren ,  Lena Hojvall

发明人： Per Mansson ,  Daniel Berggren ,  Carl Lundberg ,  Ann-Charlotte Hellgren ,  Lena Hojvall

IPC分类号： C07C211/00 ,  C07C331/00 ,  C07D221/22 ,  C07D251/00 ,  C07D257/08 ,  C07D317/44 ,  C07D451/00 ,  C07F15/00 ,  C07H19/00 ,  C07H21/02 ,  C07K14/00 ,  C07K16/00 ,  C07K2/00 ,  C12M3/00 ,  C12N1/00 ,  C12N1/12 ,  C12N1/20 ,  C12N7/00 ,  C12P21/08

CPC分类号： G01N29/022 ,  G01N29/222 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0426

摘要： A mixture of isolated or synthetic affinity molecules in a liquid carrier is disclosed. The mixture comprises at least two different affinity molecules, each with affinity for a predetermined analyte, for use in a single or multi flow cell piezoelectric crystal micro balance apparatus. Each isolated or synthetic affinity molecule forms together with the predetermined analyte an interaction pair selected from the group consisting of anion-cation, antibody-antigen, receptor-ligand, enzyme-substrate, oligonucleotide-oligonucleotide with complementary sequence, oligonucleotide-protein, oligonucleotide-cell, and peptide nucleic acid (PNA) oligomer-polynucleotide, wherein the polynucleotide may be selected from the group consisting of RNA, DNA and PNA polymers complementary to PNA oligomer. Use of the mixture for introduction into the liquid flow of a single or multi flow cell piezoelectric crystal micro balance apparatus is also described, as well as a kit containing the mixture.

摘要翻译： 公开了液体载体中分离的或合成的亲和分子的混合物。 该混合物包含至少两种不同的亲和力分子，各自对于预定的分析物具有亲和力，用于单流或多流量单元压电晶体微量平衡装置。 每个孤立或合成的亲和分子与预定分析物一起形成选自阴离子阳离子，抗体 - 抗原，受体 - 配体，酶 - 底物，具有互补序列的寡核苷酸寡核苷酸，寡核苷酸 - 蛋白，寡核苷酸 - 细胞和肽核酸（PNA）寡聚体 - 多核苷酸，其中所述多核苷酸可以选自与PNA低聚物互补的RNA，DNA和PNA聚合物。 还描述了将混合物引入单流动池或多流通池压电晶体微量平衡装置的液体流中的用途，以及含有该混合物的试剂盒。

公开(公告)号：US5429838A

公开(公告)日：1995-07-04

申请号：US131851

申请日：1993-10-05

申请人： Per Mansson ,  Johannes Hermans

发明人： Per Mansson ,  Johannes Hermans

IPC分类号： G02C7/04 ,  A61F2/16 ,  B29C39/02 ,  B29D11/00 ,  B29D11/02 ,  G02B1/10 ,  A61F2/14

CPC分类号： B29D11/00865 ,  B29L2011/0016

摘要： In a method for producing intraocular lenses with improved optical properties, applying to the optical part of the lens a coating to protect the surface during one or more processing steps which are carried out under conditions so that the protective coating is essentially insoluble and remains adhered to the lens, and removing the coating by changing the conditions which make the coating adhere to the lens.

摘要翻译： 在用于生产具有改善的光学性质的眼内透镜的制造方法中，在一个或多个加工步骤期间，将镜片的光学部件涂覆到一个或多个加工步骤中以保护表面的涂层，所述加工步骤在保护涂层基本上不溶和保持粘附的条件下进行 透镜，并通过改变使涂层粘附到透镜上的条件来去除涂层。

公开(公告)号：US20090168068A1

公开(公告)日：2009-07-02

申请号：US12289218

申请日：2008-10-23

申请人： Per Mansson ,  Bo Liedberg

发明人： Per Mansson ,  Bo Liedberg

IPC分类号： G01N21/55 ,  G01N33/553 ,  B05D1/36 ,  B32B15/04

CPC分类号： B82Y30/00 ,  B82Y15/00 ,  G01N33/54353 ,  G01N2610/00 ,  Y10T428/31678

摘要： A coated metal surface on a solid support, wherein the coating consists of a self-assembled monolayer (SAM) of oligo(ethylene glycol)-terminated amide group-containing alkyl thiols firmly attached to the metal surface via the thiol-end and low molecular weight antigens bound via an amide-group to the SAM-forming OEG molecule, wherein the alkyl portion has 1-20 methylene groups, wherein the oligo(ethylene glycol) portion has 1-15 ethylene oxy units, and wherein the antigens, such as explosives and narcotics, are optionally reversibly bound to antibodies specific for the antigens, is disclosed. The coated metal surface on a solid support may be used in a method of detecting analyte antigens as part of an analysis device, such as a Piezoelectric Crystal Microbalance device or a Surface Plasmon Resonance biosensor, for detection in an aqueous solution of an analyte antigen with higher affinity to an antibody than the antigen of the coating by monitoring the displacement of the antibody from the coating.

摘要翻译： 固体载体上的涂覆的金属表面，其中涂层由通过硫醇末端和低分子量牢固地连接到金属表面的低聚（乙二醇）封端的含酰胺基的烷基硫醇的自组装单层（SAM）组成 通过酰胺基与SAM形成的OEG分子结合的重量抗原，其中所述烷基部分具有1-20个亚甲基，其中所述低聚（乙二醇）部分具有1-15个乙烯氧基单元，并且其中所述抗原，例如 爆炸物和麻醉剂任选地可逆地结合抗原特异性抗体。 固体支持物上的涂覆的金属表面可以用于检测作为分析装置的一部分的分析物抗原的方法，例如压电晶体微量天平装置或表面等离子体共振生物传感器，用于在分析物抗原的水溶液中检测， 通过监测来自涂层的抗体的置换，对抗体比涂层的抗原更高的亲和性。

公开(公告)号：US20060014270A1

公开(公告)日：2006-01-19

申请号：US10517321

申请日：2003-06-18

申请人： Per Mansson ,  Henrik Andersson ,  Jan Smith ,  Kjeld Jensen ,  Teodor Aastrup

发明人： Per Mansson ,  Henrik Andersson ,  Jan Smith ,  Kjeld Jensen ,  Teodor Aastrup

IPC分类号： C12M1/34

CPC分类号： G01N35/1097 ,  G01N5/02 ,  G01N29/036 ,  G01N33/946 ,  G01N2291/106

摘要： A system, device and method for detection of several individual analytes in a test solution aliquot (83) with an array of individually operated piezoelectric crystal microbalances are described. The system comprises a connecting station (100) that receives a plurality of individually specific piezoelectric crystal microbalance flow-trough cells (10), each containing a piezoelectric crystal (50) carrying electrodes (56,62) and a coating (66,46) exposing a first member of an interaction pair specific for an individual analyte being a second member of the interaction pair. Flowing means (70) flows a solution (75) and the test solution aliquot (83) to and through a cell compartment of each of the cells (10) via the connecting station (100). Power and measurement means (130) oscillate the piezoelectric crystal(s) (50). A change in oscillating characteristics of the crystal(s) (50), following interaction between the first and second members of the interaction pair, detects presence of the individual analyte(s). Further, disposable flow cells (10) for use in an array of individually operated piezoelectric crystal microbalances (112) is described.

摘要翻译： 描述了具有独立操作的压电晶体微量平衡阵列的测试溶液等分试样（83）中检测几种单独分析物的系统，装置和方法。 该系统包括接收多个单独特定的压电晶体微量天平流槽电池（10）的连接站（100），每个单元包含承载电极（56,62）和涂层（66,46）的压电晶体（50） 暴露对于作为相互作用对的第二成员的单个分析物特异性的相互作用对的第一成员。 流动装置（70）通过连接站（100）将溶液（75）和测试溶液等分试样（83）流经并通过每个电池（10）的电池室。 功率和测量装置（130）振荡压电晶体（50）。 在相互作用对的第一和第二成员之间的相互作用之后，晶体（50）的振荡特性的变化检测各个分析物的存在。 此外，描述了用于独立操作的压电晶体微量平衡阵列（112）的一次性流通池（10）。

公开(公告)号：US20120040473A1

公开(公告)日：2012-02-16

申请号：US13147251

申请日：2009-01-30

申请人： Per Mansson

发明人： Per Mansson

IPC分类号： G01N29/02

CPC分类号： G01N29/022 ,  G01N29/245 ,  G01N33/54373 ,  G01N2291/0224 ,  G01N2291/02809

摘要： A method of individually analyzing with a surface acoustic wave (SAW) sensor apparatus the presence or absence of at least two different target antigens in a liquid sample is described. The method comprises providing and running a single flow cell comprising a SAW sensor chip with at least two, e.g. twelve, SAW sensor elements, each comprising an individual coating immobilizing and exposing a modified antigen that has a weaker affinity for an antibody which is specific for one of the target antigens than the target antigen itself. Such a flow cell and surface acoustic wave (SAW) sensor chip for immunological displacement reactions are also disclosed.

摘要翻译： 描述了使用表面声波（SAW）传感器装置单独分析液体样品中存在或不存在至少两种不同靶抗原的方法。 该方法包括提供和运行包括SAW传感器芯片的单个流动池，其具有至少两个，例如， 十二，SAW传感器元件，每个包括单独的涂层，固定和暴露对靶抗原本身具有特异性的抗体具有较弱亲和力的修饰抗原。 还公开了用于免疫位移反应的这种流动池和表面声波（SAW）传感器芯片。

公开(公告)号：US20070163327A1

公开(公告)日：2007-07-19

申请号：US10557598

申请日：2004-05-18

申请人： Per Mansson

发明人： Per Mansson

IPC分类号： G01N1/40 ,  G01N1/22 ,  G01N33/15 ,  B03C3/38

CPC分类号： G01N1/40 ,  G01N2001/022

摘要： A system and a method for detection of trace amounts of airborne or deposited low-molecular weight compound(s), such as narcotics or explosives, comprising an electrically charged collector surface connected to an ionizer ionizing airborne particles with an electrical charge opposite to the charge of the collector surface, optional air-stirring means, extraction means for extraction of collected material from the collector surface, and equipment for chemical and/or biochemical detection of said compound(s) from said extracted collected material, is disclosed. Further, a modified ionizer/collector device wherein the collector is extended from the body of the device and is equipped with a preferably detachable, electrically chargeable collecting surface and wherein optionally the air-ionizing part of the device is also extended from the body of the ionizer/collector device, either together with or apart from, the extended collector arm, is described. The device may be battery-driven and portable, and suitable for use by the customs, airport security and police for the detection of e.g. narcotics or explosives or in mine detection.

摘要翻译： 用于检测微量空气传播或沉积的低分子量化合物（例如麻醉剂或爆炸物）的系统和方法，包括连接到电离器的带电收集器表面的电离电离颗粒与电荷相反的电荷 的收集器表面，可选的空气搅拌装置，用于从收集器表面提取收集的材料的提取装置，以及用于从所述提取的收集的材料进行化学和/或生物化学检测所述化合物的设备。 此外，改进的电离器/收集器装置，其中收集器从装置的主体延伸并且装备有优选可拆卸的可充电收集表面，并且其中可选地，装置的空气电离部分也从 描述了与扩展的收集器臂一起或分开的离子发生器/收集器装置。 该装置可以是电池驱动的并且是便携式的，并且适合于海关，机场安全和警察使用以检测例如。 麻醉品或炸药或矿山检测。