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公开(公告)号：US20130102067A1
公开(公告)日：2013-04-25
申请号：US13806505
申请日：2011-04-05
IPC分类号： G01N21/47
CPC分类号： G01N21/47 , C12M41/46 , G01N21/51 , G01N2021/058 , G01N2021/4707
摘要： A device for monitoring test cells has at least one receiving unit for the test cells and a first measuring unit for cell measurement. With a second measuring unit, which has a light source and a scattered light detector, cell monitoring can be carried out during the cell measurement. For this purpose the receiving unit has an at least partially light-permeable substrate and is arranged between the light source and scattered light detector such that at least a part of the light generated by the light source shines on the receiving unit, is scattered on the test cells and, after leaving the receiving unit through the substrate, impinges on the scattered light detector.
摘要翻译： 用于监测测试单元的装置具有用于测试单元的至少一个接收单元和用于单元测量的第一测量单元。 利用具有光源和散射光检测器的第二测量单元，可以在电池测量期间进行电池监测。 为此目的，接收单元具有至少部分透光的基板，并且布置在光源和散射光检测器之间，使得由光源产生的光的至少一部分照射在接收单元上，散射在 在离开接收单元通过基板之后，撞击在散射光检测器上。
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公开(公告)号：US20140356891A1
公开(公告)日：2014-12-04
申请号：US14238943
申请日：2012-08-01
CPC分类号： G01N15/1031 , G01N33/54326 , G01N33/585 , G01N33/80 , G01N2015/0065 , G01N2015/1006 , G01R33/1269
摘要： Devices and methods for magnetic flow measurement are provided. Individual analytes are detected in the through-flow, and a dynamic detection of a changing analyte state is carried out, for example with respect to the analyte size or morphology. For this purpose, the analytes to be detected, such as cells for example, are directly marked in the medium surrounding said analytes with magnetic labels and transported through the flow channel of a measuring device comprising at least two magnetic sensors. A characteristic measurement signal is generated by means of the magnetic sensors that are mutually spaced in the flow direction. The magnetic analyte diameter is calculated using the interval between the measurement deflections, and the analyte state can be evaluated using the magnetic analyte diameter.
摘要翻译： 提供磁流测量的装置和方法。 在通流中检测到各种分析物，并且例如相对于分析物的大小或形态，进行改变的分析物状态的动态检测。 为此，待检测的分析物，例如细胞，直接用磁性标记在包围所述分析物的培养基中，并通过包含至少两个磁性传感器的测量装置的流动通道传送。 通过在流动方向上相互间隔的磁性传感器产生特征测量信号。 使用测量偏转之间的间隔计算磁分析物直径，并且可以使用磁性分析物直径来评估分析物状态。
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公开(公告)号：US20130330828A1
公开(公告)日：2013-12-12
申请号：US14002071
申请日：2012-02-28
CPC分类号： G01N15/1404 , G01N1/40 , G01N33/54333 , G01N33/569 , G01N33/585 , G01N35/0098 , H01L43/12
摘要： A measuring device for magnetic flow cytometry has a microfluidic channel disposed along an enriching route such that a magnetically marked cell sample flowing through the microfluidic channel is aligned to magnetic guide strips, enriched by the magnetic field of a magnet at the floor of the channel, and guided past a sensor. The sensor and the magnetic guide strips are integrated on a semiconductor chip.
摘要翻译： 用于磁流式细胞术的测量装置具有沿着富集途径设置的微流体通道，使得流过微流体通道的磁标记细胞样品与通过通道底部的磁体的磁场富集的导磁条对准， 并经过传感器。 传感器和导磁条集成在半导体芯片上。
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公开(公告)号：US09316575B2
公开(公告)日：2016-04-19
申请号：US13820866
申请日：2011-08-29
CPC分类号： G01N15/1031
摘要： The disclosure relates to flow cytometry. A method for precise individual cell detection and cell measurement of cells in the flow is disclosed. A pair of magnetoresistive components are used to produce a characteristic measuring signal profile from which the following information can be obtained: number of measurement deviations, measurement deviation distances, measurement deviation amplitudes, measurement deviation direction and measurement deviation direction sequence. The flow speed and the cell diameter can also be determined. Also, the signal noise ratio can be determined using the measurement deviation amplitude.
摘要翻译： 本公开涉及流式细胞术。 公开了一种精确的单个细胞检测和流中的细胞的细胞测量的方法。 使用一对磁阻部件来产生特征测量信号轮廓，可从中获得以下信息：测量偏差数，测量偏差距离，测量偏差幅度，测量偏差方向和测量偏差方向序列。 也可以确定流速和细胞直径。 此外，可以使用测量偏差幅度来确定信号噪声比。
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公开(公告)号：US20130224762A1
公开(公告)日：2013-08-29
申请号：US13883497
申请日：2011-10-28
IPC分类号： G01N27/72
CPC分类号： G01N27/72 , B03C1/02 , G01N27/745 , G01N33/54326
摘要： Specific labeling of cells enables magnetic cell detection. The cell type to be detected is labeled, magnetic labels being bound to epitopes of a first cell-specific epitope type via antibodies of a first antibody type. Additionally, second/further magnetic labels are bound to epitopes of a second cell-specific epitope type on the cells via antibodies of a second antibody type, or the magnetic labels are bound to the antibodies of the first antibody type via antibodies of another antibody type and the antibodies of the first antibody type are bound to the epitopes of the first cell-specific epitope type on the cells.
摘要翻译： 细胞的特异性标记可以进行磁性细胞检测。 要检测的细胞类型被标记，磁性标记通过第一抗体类型的抗体与第一细胞特异性表位型的表位结合。 此外，第二/另外的磁性标记通过第二抗体类型的抗体与细胞上的第二细胞特异性表位类型的表位结合，或者磁性标记通过另一抗体类型的抗体与第一抗体类型的抗体结合 并且第一抗体类型的抗体结合到细胞上的第一细胞特异性表位型的表位。
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公开(公告)号：US20120182007A1
公开(公告)日：2012-07-19
申请号：US13499603
申请日：2010-08-17
申请人： Ludwig Bär , Helmut Eckert , Oliver Hayden , Sandro Francesco Tedde , Michael Vieth , Roland Weiss
发明人： Ludwig Bär , Helmut Eckert , Oliver Hayden , Sandro Francesco Tedde , Michael Vieth , Roland Weiss
IPC分类号： G01N27/74
CPC分类号： G01N15/0656 , B01L3/502761 , B01L2300/0851 , B01L2300/0877 , B01L2300/089 , B01L2400/043 , B03C1/01 , B03C1/288 , B03C2201/18 , B03C2201/20 , B03C2201/24 , B82Y25/00 , G01N2035/00564 , G01R33/093 , G01R33/1207
摘要： Magnetically labeled cells in a flow chamber cytometer are detected by a GMR sensor. The flow chamber includes a cell guiding device having at least one first and one second magnetic or magnetizable flow strip. The flow strips, which serve to guide the flowing cells across the sensor in a target-oriented manner, are mounted at a distance from each other such that a magnetic field BF is produced between them. The GMR sensor is arranged in the region of the magnetic field BF between the flow strips such that the magnetic field BF can be used as the operating magnetic field BGMR of the GMR sensor. In this way, the need for additional magnets for operating the GMR sensor is eliminated.
摘要翻译： 流式细胞仪中的磁性标记细胞由GMR传感器检测。 流动室包括具有至少一个第一和一个第二磁性或可磁化流动条的电池引导装置。 用于以目标取向的方式引导流动的细胞穿过传感器的流动条彼此间隔一定距离地安装，使得它们之间产生磁场BF。 GMR传感器布置在流动条之间的磁场BF的区域中，使得磁场BF可以用作GMR传感器的操作磁场BGMR。 以这种方式，消除了用于操作GMR传感器的附加磁体的需要。
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公开(公告)号：US10739235B2
公开(公告)日：2020-08-11
申请号：US13383072
申请日：2010-05-14
摘要： A flexible, thin, elongated band is used as a substrate. Similarly to a magnetic tape, the band is unwound from a feed reel and is transported past an outlet opening of a receptacle containing the cells such that the cells are poured onto the band. Subsequently, the band containing the cells applied thereon is wound onto a take-up reel. The take-up reel is fixed on a drive shaft which can be rotated by a drive mechanism. The rotation thus achieved has the effect that the band is unwound, transported past the outlet opening and finally wound up. In addition, spacers are provided at the upper surface of the band in order to prevent the contacting of radially adjacent sections of the band containing the cells in a wound-up state.
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公开(公告)号：US09651470B2
公开(公告)日：2017-05-16
申请号：US14235449
申请日：2012-07-24
IPC分类号： G01N33/543 , G01N33/553 , G01N15/10 , B01L3/00
CPC分类号： G01N15/1031 , B01L3/502746 , B01L3/502761 , B01L3/502776 , B01L2300/0816 , B01L2400/043 , B01L2400/086
摘要： The invention relates to a device and a method for fluidic cell guidance for flow cytometry or analyte enrichment. This allows magnetically marked analytes, in particular cells (1), to be dynamically enriched and individually detected in the flow from a sample, in particular magnetoresistively. For cell guidance, guiding ridges (12) are arranged in a flow channel (100), and so, in addition to a magnetic enrichment force (10z) and the shearing force of the flow (10y), a deflecting force (10x) caused by the fluidic obstacles (12) also acts on the cells (1) to be detected.
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公开(公告)号：US20130164777A1
公开(公告)日：2013-06-27
申请号：US13820866
申请日：2011-08-29
IPC分类号： G01N27/72
CPC分类号： G01N15/1031
摘要： The disclosure relates to flow cytometry. A method for precise individual cell detection and cell measurement of cells in the flow is disclosed. A pair of magnetoresistive components are used to produce a characteristic measuring signal profile from which the following information can be obtained: number of measurement deviations, measurement deviation distances, measurement deviation amplitudes, measurement deviation direction and measurement deviation direction sequence. The flow speed and the cell diameter can also be determined. Also, the signal noise ratio can be determined using the measurement deviation amplitude
摘要翻译： 本公开涉及流式细胞术。 公开了一种精确的单个细胞检测和流中的细胞的细胞测量的方法。 使用一对磁阻部件来产生特征测量信号轮廓，可从中获得以下信息：测量偏差数，测量偏差距离，测量偏差幅度，测量偏差方向和测量偏差方向序列。 也可以确定流速和细胞直径。 此外，可以使用测量偏差幅度来确定信号噪声比
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公开(公告)号：US20130004982A1
公开(公告)日：2013-01-03
申请号：US13172070
申请日：2011-06-29
申请人： Ludwig Bär , Oliver Hayden , Michael Johannes Helou , Mischa Megens , Mathias Reisbeck , Manfred Rührig , Sandro Francesco Tedde
发明人： Ludwig Bär , Oliver Hayden , Michael Johannes Helou , Mischa Megens , Mathias Reisbeck , Manfred Rührig , Sandro Francesco Tedde
CPC分类号： G01N33/587 , G01N15/1031 , G01N2015/1062
摘要： A magnetic flow cytometry apparatus for detection of cells labeled with magnetic nanoparticles has at least one pair of oppositely oriented magnets to provide between the magnets a first magnetic field region with a low magnetic field strength and to provide at poles of the magnets second magnetic field regions with a high magnetic field strength. The magnetic labeled cells provided within a flow input into the magnetic flow cytometry apparatus are enriched in at least one of the second magnetic field regions and supplied to the first magnetic field region, where a magnetic field is applied to the enriched magnetic labeled cells to measure the magnetic relaxation of the magnetic labeled cells in response to the applied magnetic field.
摘要翻译： 用于检测用磁性纳米颗粒标记的细胞的磁流式细胞仪装置具有至少一对相反方向的磁体，以在磁体之间提供具有低磁场强度的第一磁场区域，并且在磁体的极点处提供第二磁场区域 具有高磁场强度。 提供在输入到磁流式细胞计量装置的流量内的磁性标记细胞富集在第二磁场区域中的至少一个中，并被提供给第一磁场区域，其中向富集的磁性标记细胞施加磁场以测量 响应于施加的磁场，磁性标记的细胞的磁弛豫。
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