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    Filamentous Fungal Mutants With Improved Homologous Recombination Efficiency
    1.
    发明申请
    Filamentous Fungal Mutants With Improved Homologous Recombination Efficiency 有权
    具有改善的同源重组效率的丝状真菌突变体

    公开(公告)号：US20080227145A1

    公开(公告)日：2008-09-18

    申请号：US10594014

    申请日：2005-03-31

    申请人： Petrus Jacobus Theodorus Dekker ,  Marco Alexander van den Berg

    发明人： Petrus Jacobus Theodorus Dekker ,  Marco Alexander van den Berg

    IPC分类号： C12P23/00 ,  C12N15/74 ,  C12N1/00 ,  C07K14/00 ,  C12N15/11 ,  C12P21/04

    CPC分类号： C12N15/80 ,  C07K14/37 ,  C12N15/902 ,  C12P17/10 ,  C12P21/00 ,  C12P21/02 ,  C12P23/00 ,  C12P37/00

    摘要： The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions.

    摘要翻译： 本发明涉及一种提高多核苷酸靶向整合到具有优选NHR的丝状真菌细胞基因组中的预定位点的效率的方法，其中所述多核苷酸具有与所述预先确定的同源性的区域 包括指导人力资源的整合途径。 本发明还涉及源自亲本细胞的突变丝状真菌，所述突变体具有提高效率的HR途径和/或具有降低的效率和/或NHR / HR比率的NHR途径，与所述 在相同条件下所述亲本细胞的HR和/或NHR效率和/或NHR / HR比。

    NOVEL SIALIDASES
    3.
    发明申请
    NOVEL SIALIDASES 有权
    新的SIALIDASES

    公开(公告)号：US20100167344A1

    公开(公告)日：2010-07-01

    申请号：US12527064

    申请日：2008-02-18

    申请人： Albertus Alard Van Dijk ,  Natalja Alekseevna Cyplenkova ,  Petrus Jacobus Theodorus Dekker ,  Yulia M. Efimova

    发明人： Albertus Alard Van Dijk ,  Natalja Alekseevna Cyplenkova ,  Petrus Jacobus Theodorus Dekker ,  Yulia M. Efimova

    IPC分类号： C12P21/06 ,  C12N9/14 ,  C12N5/10 ,  C12N15/63 ,  C07H21/04

    CPC分类号： A23C19/0328 ,  C12N9/2402 ,  C12Y302/01018

    摘要： The present invention relates to an isolated polypeptide which has sialidase activity, selected from the group consisting of: (a) a polypeptide which has an amino acid sequence which has at least 60% amino acid sequence identity with amino acids 1 to 407 of SEQ ID NO: 3; (b) a polypeptide which is encoded by a polynucleotide which hybridizes under low stringency conditions with (i) the nucleic acid sequence of SEQ ID NO: 1 or 2 which is at least 80% or 90% identical over 60, preferably over 100 nucleotides, more preferably at least 90% identical over 200 nucleotides, or (ii) a nucleic acid sequence complementary to the nucleic acid sequence of SEQ ID NO: 1 or 2.

    摘要翻译： 本发明涉及具有唾液酸酶活性的分离多肽，其选自：（a）具有与SEQ ID NO：1的氨基酸1至407具有至少60％氨基酸序列同一性的氨基酸序列的多肽 NO：3; （b）由低度严格条件下与下列杂交的多核苷酸编码的多肽：（i）SEQ ID NO：1或2的核酸序列在60以上，优选超过100个核苷酸至少80％或90％同一性 ，更优选在200个核苷酸以上至少90％相同，或（ii）与SEQ ID NO：1或2的核酸序列互补的核酸序列。

    PROLINE-SPECIFIC PROTEASE FROM PENCILLIUM CHRYSOGENUM
    7.
    发明申请
    PROLINE-SPECIFIC PROTEASE FROM PENCILLIUM CHRYSOGENUM 审中-公开
    来自P。。。。。。。。。。。

    公开(公告)号：US20110165306A1

    公开(公告)日：2011-07-07

    申请号：US12993174

    申请日：2009-05-28

    申请人： Petrus Jacobus Theodorus Dekker ,  Luppo Edens

    发明人： Petrus Jacobus Theodorus Dekker ,  Luppo Edens

    IPC分类号： A23L2/00 ,  C12N9/58 ,  C07H21/04 ,  C12N15/63 ,  C12N15/80 ,  C12N5/10 ,  C12N1/00 ,  C12P21/06 ,  C07K2/00 ,  A23J1/00 ,  A23K1/00

    CPC分类号： C12N9/58 ,  A23K20/147 ,  A23L33/18

    摘要： A polypeptide having proline-specific protease activity which comprises the amino acid sequence set out in SEQ ID NO: 3 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1 and/or SEQ ID NO: 2, or a variant thereof or a fragment of either thereof. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

    摘要翻译： 具有脯氨酸特异性蛋白酶活性的多肽，其包含SEQ ID NO：3所示的氨基酸序列或由SEQ ID NO：1和/或SEQ ID NO：2的核苷酸序列编码的氨基酸序列，或变体 或其任何一个片段。 本发明还涉及在工业过程中使用该多肽的方法。 本发明还包括用适合于生产这些蛋白质的根据本发明的多核苷酸转化的细胞。