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1.发明授权Methods and systems to prevent punch loss during automated sample processing 有权自动化样品处理过程中防止冲孔损失的方法和系统公开(公告)号：US08689649B2
公开(公告)日：2014-04-08
申请号：US12630899
申请日：2009-12-04
IPC分类号： G01N1/08
CPC分类号： G01N1/286 , G01N2001/288 , G01N2035/042
摘要： Automated methods and systems for punching out pieces of a porous substrate for biological samples comprising: loading the porous substrate onto a support comprising a die and an opening; moving a receptacle support in at least a z-direction to position a receptacle relative to the support so that an opening in the receptacle is aligned and substantially flush with the opening in the support; actuating a punching head so that the punching head passes through the die, thereby punching a piece out of the porous substrate; and actuating an ejector pin to eject the punched piece from the porous substrate support and into the receptacle aligned with the opening in the support.
摘要翻译： 用于冲切用于生物样品的多孔基材片的自动方法和系统，包括：将所述多孔基材装载到包括模头和开口的支撑件上; 在至少z方向上移动容器支撑件以相对于支撑件定位容器，使得容器中的开口对齐并且基本上与支撑件中的开口齐平; 致动冲压头，使冲头通过冲模，从而从多孔基材上冲出一块; 并且致动顶针以将冲压件从多孔基底支撑件推出并进入与支撑件中的开口对准的容器中。
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2.发明申请METHODS AND SYSTEMS TO PREVENT PUNCH LOSS DURING AUTOMATED SAMPLE PROCESSING 有权在自动样品加工过程中防止冲击损失的方法和系统公开(公告)号：US20110132111A1
公开(公告)日：2011-06-09
申请号：US12630899
申请日：2009-12-04
IPC分类号： G01N1/04
CPC分类号： G01N1/286 , G01N2001/288 , G01N2035/042
摘要： Automated methods and systems for punching out pieces of a porous substrate for biological samples comprising: loading the porous substrate onto a support comprising a die and an opening; moving a receptacle support in at least a z-direction to position a receptacle relative to the support so that an opening in the receptacle is aligned and substantially flush with the opening in the support; actuating a punching head so that the punching head passes through the die, thereby punching a piece out of the porous substrate; and actuating an ejector pin to eject the punched piece from the porous substrate support and into the receptacle aligned with the opening in the support.
摘要翻译： 用于冲切用于生物样品的多孔基材片的自动方法和系统，包括：将所述多孔基材装载到包括模头和开口的支撑件上; 在至少z方向上移动容器支撑件以相对于支撑件定位容器，使得容器中的开口对齐并且基本上与支撑件中的开口齐平; 致动冲压头，使冲头通过冲模，从而从多孔基材上冲出一块; 并且致动顶针以将冲压件从多孔基底支撑件推出并进入与支撑件中的开口对准的容器中。
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公开(公告)号：US20110129863A1
公开(公告)日：2011-06-02
申请号：US12629088
申请日：2009-12-02
申请人： Philip Alexander Shoemaker , Weston Blaine Griffin , Erin Jean Finehout , Xuefeng Wang , Kashan Ali Shaikh , Greg Darryl Goddard
发明人： Philip Alexander Shoemaker , Weston Blaine Griffin , Erin Jean Finehout , Xuefeng Wang , Kashan Ali Shaikh , Greg Darryl Goddard
CPC分类号： G01N1/4055 , B01L3/5025 , B01L3/50255 , B01L2200/025 , B01L2200/0689 , B01L2300/0627 , B01L2300/0829 , B01L2300/0832 , G01N1/34 , G01N1/405 , G01N1/4077 , G01N30/7233 , G01N35/00029 , G01N35/1095 , G01N2001/4061 , G01N2001/4088 , G01N2030/009 , G01N2030/062 , G01N2035/00128 , Y10T436/24 , Y10T436/25 , Y10T436/25375 , Y10T436/255
摘要： Methods and systems for processing samples fixed to a porous substrate generally comprising, a compressor defining one or more fluid isolation areas, a support, for the porous substrate, having an opening corresponding to one or more of the fluid isolation areas of the compressor, an actuator that causes at least a portion of the compressor to press against the porous substrate, a fluid inlet having access to the fluid isolation area at least when the compressor is pressed against the porous substrate, and a fluid outlet to receive fluid, through the opening in the support corresponding to the fluid isolation area of the compressor, at least when the compressor is pressed against the porous substrate.
摘要翻译： 用于处理固定到多孔基材的样品的方法和系统通常包括限定一个或多个流体隔离区域的压缩机，用于多孔基材的支撑体，其具有对应于压缩机的一个或多个流体隔离区域的开口， 致动器，其使至少一部分压缩机压靠多孔基底，至少当压缩机被压靠在多孔基底上时，流体入口至少进入流体隔离区域，以及流体出口以接收流体，通过开口 在对应于压缩机的流体隔离区域的支撑件中，至少当压缩机被压靠在多孔基底上时。
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公开(公告)号：US08685749B2
公开(公告)日：2014-04-01
申请号：US12629088
申请日：2009-12-02
申请人： Philip Alexander Shoemaker , Weston Blaine Griffin , Erin Jean Finehout , Xuefeng Wang , Kashan Ali Shaikh , Greg Darryl Goddard
发明人： Philip Alexander Shoemaker , Weston Blaine Griffin , Erin Jean Finehout , Xuefeng Wang , Kashan Ali Shaikh , Greg Darryl Goddard
CPC分类号： G01N1/4055 , B01L3/5025 , B01L3/50255 , B01L2200/025 , B01L2200/0689 , B01L2300/0627 , B01L2300/0829 , B01L2300/0832 , G01N1/34 , G01N1/405 , G01N1/4077 , G01N30/7233 , G01N35/00029 , G01N35/1095 , G01N2001/4061 , G01N2001/4088 , G01N2030/009 , G01N2030/062 , G01N2035/00128 , Y10T436/24 , Y10T436/25 , Y10T436/25375 , Y10T436/255
摘要： Methods and systems for processing samples fixed to a porous substrate generally comprising, a compressor defining one or more fluid isolation areas, a support, for the porous substrate, having an opening corresponding to one or more of the fluid isolation areas of the compressor, an actuator that causes at least a portion of the compressor to press against the porous substrate, a fluid inlet having access to the fluid isolation area at least when the compressor is pressed against the porous substrate, and a fluid outlet to receive fluid, through the opening in the support corresponding to the fluid isolation area of the compressor, at least when the compressor is pressed against the porous substrate.
摘要翻译： 用于处理固定到多孔基材的样品的方法和系统通常包括限定一个或多个流体隔离区域的压缩机，用于多孔基材的支撑体，其具有对应于压缩机的一个或多个流体隔离区域的开口， 致动器，其使至少一部分压缩机压靠多孔基底，至少当压缩机被压靠在多孔基底上时，流体入口至少进入流体隔离区域，以及流体出口以接收流体，通过开口 在对应于压缩机的流体隔离区域的支撑件中，至少当压缩机被压靠在多孔基底上时。
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公开(公告)号：US07740748B2
公开(公告)日：2010-06-22
申请号：US12258657
申请日：2008-10-27
申请人： Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout , Jun Xie
发明人： Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout , Jun Xie
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： B01D57/02 , B01L3/502715 , G01N27/44743
摘要： Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis device comprises a loading channel, an injection channel, and a separation channel. The loading channel is in fluid communication with a first and second sample port. The injection channel is connected to the loading channel to form a first intersection. The separation channel is connected to the injection channel to form a second intersection and in fluid communication with a first and second reservoir, and wherein the injection channel is in fluid communication with a third reservoir. The electrophoresis system further comprises electrodes coupled to the first sample port and the third reservoir, and the first reservoir and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the third reservoir and form a sample plug in the second intersection, and to further move the sample plug into the separation channel towards the second reservoir.
摘要翻译： 电泳系统和方法包括电泳装置，其中电泳装置包括装载通道，注射通道和分离通道。 装载通道与第一和第二采样端口流体连通。 注入通道连接到装载通道以形成第一交叉。 分离通道连接到注入通道以形成第二交叉并且与第一和第二储存器流体连通，并且其中注入通道与第三储存器流体连通。 电泳系统还包括分别耦合到第一样品端口和第三储存器的电极，以及分别适于将样品移动到装载通道中的第一储存器和第二储存器，以朝向第三储存器形成样品塞 并且将样品塞进一步朝向第二储存器移动到分离通道中。
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公开(公告)号：US20100101951A1
公开(公告)日：2010-04-29
申请号：US12258657
申请日：2008-10-27
申请人： Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout , Jun Xie
发明人： Wei-Cheng Tian , Shashi Thutupalli , Li Zhu , Anthony John Murray , Erin Jean Finehout , Jun Xie
IPC分类号： B01D57/02
CPC分类号： B01D57/02 , B01L3/502715 , G01N27/44743
摘要： Electrophoresis systems and methods comprise an electrophoresis device, wherein the electrophoresis device comprises a loading channel, an injection channel, and a separation channel. The loading channel is in fluid communication with a first and second sample port. The injection channel is connected to the loading channel to form a first intersection. The separation channel is connected to the injection channel to form a second intersection and in fluid communication with a first and second reservoir, and wherein the injection channel is in fluid communication with a third reservoir. The electrophoresis system further comprises electrodes coupled to the first sample port and the third reservoir, and the first reservoir and the second reservoir, respectively, that are adapted to move the sample into the loading channel towards the third reservoir and form a sample plug in the second intersection, and to further move the sample plug into the separation channel towards the second reservoir.
摘要翻译： 电泳系统和方法包括电泳装置，其中电泳装置包括装载通道，注射通道和分离通道。 装载通道与第一和第二采样端口流体连通。 注入通道连接到装载通道以形成第一交叉。 分离通道连接到注入通道以形成第二交叉并且与第一和第二储存器流体连通，并且其中注入通道与第三储存器流体连通。 电泳系统还包括分别耦合到第一样品端口和第三储存器的电极，以及分别适于将样品移动到装载通道中的第一储存器和第二储存器，以朝向第三储存器形成样品塞 并且将样品塞进一步朝向第二储存器移动到分离通道中。
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公开(公告)号：US20080308422A1
公开(公告)日：2008-12-18
申请号：US11762564
申请日：2007-06-13
CPC分类号： G01N27/44704 , G01N1/38 , Y10T436/108331
摘要： In one aspect, the invention provides methods for protein sample preparation for electrophoretic separation. The method comprises a step of providing a protein sample in solution, adding a chaotrope to the protein sample, adding a surfactant to the protein sample, wherein the final concentration of the surfactant in the solution is less than critical micelle concentration of the surfactant. In another aspect, the invention also provides methods of electrophoretic separation of protein samples that includes the protein sample preparation method as described herein. In yet another aspect, the invention provides a loading buffer solution composition for protein sample preparation that includes a surfactant in solution at a final concentration that is less than critical micelle concentration of the surfactant, and; a chaotrope.
摘要翻译： 一方面，本发明提供了用于电泳分离的蛋白质样品制备方法。 该方法包括在溶液中提供蛋白质样品，向蛋白质样品中加入离液剂，向蛋白质样品中加入表面活性剂的步骤，其中溶液中表面活性剂的最终浓度小于表面活性剂的临界胶束浓度。 另一方面，本发明还提供了包括如本文所述的蛋白质样品制备方法的蛋白质样品的电泳分离方法。 另一方面，本发明提供了用于蛋白质样品制备的负载缓冲溶液组合物，其包含溶液中的表面活性剂，其终浓度小于表面活性剂的临界胶束浓度， 一种离液剂
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8.发明申请DEVICES AND SYSTEMS FOR ISOLATING BIOMOLECULES AND ASSOCIATED METHODS THEREOF 审中-公开用于分离生物分子的装置和系统及其相关方法公开(公告)号：US20150259671A1
公开(公告)日：2015-09-17
申请号：US13562947
申请日：2012-07-31
申请人： Christopher Michael Puleo , Ralf Lenigk , John Richard Nelson , Xiaohui Chen , Li Zhu , Erin Jean Finehout , Patrick McCoy Spooner
发明人： Christopher Michael Puleo , Ralf Lenigk , John Richard Nelson , Xiaohui Chen , Li Zhu , Erin Jean Finehout , Patrick McCoy Spooner
IPC分类号： C12N15/10
CPC分类号： C12N15/101 , B01L3/50273 , B01L3/502738 , B01L2200/0631 , B01L2200/16 , B01L2300/0816 , B01L2300/0867 , B01L2400/0418 , B01L2400/0487 , B01L2400/0683
摘要： A device, a system, a cartridge and a method for isolating biomolecules from biological materials are provided. The device comprises a substrate; a reagent storage location; and a self-rupturing component comprising a fluid and a pressure source embedded therein, wherein the substrate, the reagent storage location and the self-rupturing component are operationally coupled to each other. A system is provided, wherein the system comprises an extraction matrix, an enclosed matrix housing comprising a biological sample inlet, one or more biomolecule extraction reagents to extract biomolecules and at least one pressure source embedded therein, a fluidic extraction circuit; and a controller for activating the embedded pressure source. A method of isolating nucleic acids from biological materials is also provided.
摘要翻译： 提供了一种用于从生物材料中分离生物分子的装置，系统，盒和方法。 该装置包括基板; 试剂储存位置; 以及包含嵌入其中的流体和压力源的自破裂部件，其中所述基板，所述试剂储存位置和所述自破裂部件在操作上彼此耦合。 提供了一种系统，其中所述系统包括提取矩阵，包括生物样品入口的封闭矩阵壳体，用于提取生物分子的一种或多种生物分子提取试剂和嵌入其中的至少一种压力源，流体萃取回路; 以及用于启动嵌入式压力源的控制器。 还提供了从生物材料中分离核酸的方法。
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公开(公告)号：US07927476B2
公开(公告)日：2011-04-19
申请号：US12341233
申请日：2008-12-22
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： B01D57/02
摘要： Methods and systems for injecting a sample during electrophoresis, that generally comprise: loading a sieving matrix through a first end of a separation channel; having the an end of the sieving matrix at a set distance from the intersection of the separation channel and a loading channel; pressure loading a sample through the loading channel and filling an empty portion of the separation channel; applying an electric field across the separation channel while flowing a washing buffer through the loading channel; and injecting a portion of the sample into the separation channel, wherein the portion of the sample injected is of a size that is determined by a distance between the end of the sieving matrix and the intersection of the loading and separation channels.
摘要翻译： 用于在电泳期间注射样品的方法和系统，其通常包括：通过分离通道的第一端装载筛分基质; 筛分矩阵的结束距离分离通道和加载通道的交点处一定距离; 通过加载通道加载样品并填充分离通道的空的部分; 在使洗涤缓冲液流过所述装载通道的同时，在所述分离通道上施加电场; 以及将所述样品的一部分注入所述分离通道中，其中所述注入的所述样品的所述部分具有由所述筛分矩阵的末端与所述加载和分离通道的交点之间的距离确定的尺寸。
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公开(公告)号：US20090166203A1
公开(公告)日：2009-07-02
申请号：US11965794
申请日：2007-12-28
申请人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
发明人： Wei-Cheng Tian , Erin Jean Finehout , Li Zhu , Jun Xie , Shashi Thutupalli
IPC分类号： G01N27/26 , G01N27/447
CPC分类号： G01N27/44743
摘要： A microchip for capillary electrophoresis is provided. The microchip comprises an injection channel and a separation channel configured to receive a sample through a sample well disposed on a first end of the separation channel; wherein the injection channel and the separation channel intersect to form a ‘T’ junction. The microchip further comprises a first valve disposed adjacent to the ‘T’ junction and on the separation channel and a second valve disposed at the ‘T’ junction. The second valve is a two-way valve. A sample plug is injected into an area between the ‘T’ junction and a second end of the separation channel.
摘要翻译： 提供了用于毛细管电泳的微芯片。 微芯片包括注入通道和分离通道，其被配置为通过设置在分离通道的第一端上的样品阱接收样品; 其中注入通道和分离通道相交以形成“T”结。 所述微芯片还包括邻近所述“T”结和所述分离通道设置的第一阀，以及设置在所述“T”结处的第二阀。 第二个阀是一个二通阀。 将样品塞注入到“T”结和分离通道的第二端之间的区域中。
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