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公开(公告)号：US20140001337A1
公开(公告)日：2014-01-02
申请号：US13538898
申请日：2012-06-29
IPC分类号： H01L27/146
CPC分类号： G01N1/312
摘要： A method for processing and imaging a first and second plurality of samples, comprising processing at least one sample from the first plurality of samples, imaging the at least one sample from the first plurality of samples, while being capable of simultaneously processing at least one sample from the second plurality of samples; and imaging the at least one processed sample from the second plurality of samples.
摘要翻译： 一种用于处理和成像第一和第二多个样品的方法，包括处理来自所述第一多个样品的至少一个样品，对来自所述第一多个样品的所述至少一个样品进行成像，同时能够同时处理至少一个样品 从第二多个样本; 以及对来自所述第二多个样本的所述至少一个处理的样品进行成像。
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公开(公告)号：US08685749B2
公开(公告)日：2014-04-01
申请号：US12629088
申请日：2009-12-02
申请人： Philip Alexander Shoemaker , Weston Blaine Griffin , Erin Jean Finehout , Xuefeng Wang , Kashan Ali Shaikh , Greg Darryl Goddard
发明人： Philip Alexander Shoemaker , Weston Blaine Griffin , Erin Jean Finehout , Xuefeng Wang , Kashan Ali Shaikh , Greg Darryl Goddard
CPC分类号： G01N1/4055 , B01L3/5025 , B01L3/50255 , B01L2200/025 , B01L2200/0689 , B01L2300/0627 , B01L2300/0829 , B01L2300/0832 , G01N1/34 , G01N1/405 , G01N1/4077 , G01N30/7233 , G01N35/00029 , G01N35/1095 , G01N2001/4061 , G01N2001/4088 , G01N2030/009 , G01N2030/062 , G01N2035/00128 , Y10T436/24 , Y10T436/25 , Y10T436/25375 , Y10T436/255
摘要： Methods and systems for processing samples fixed to a porous substrate generally comprising, a compressor defining one or more fluid isolation areas, a support, for the porous substrate, having an opening corresponding to one or more of the fluid isolation areas of the compressor, an actuator that causes at least a portion of the compressor to press against the porous substrate, a fluid inlet having access to the fluid isolation area at least when the compressor is pressed against the porous substrate, and a fluid outlet to receive fluid, through the opening in the support corresponding to the fluid isolation area of the compressor, at least when the compressor is pressed against the porous substrate.
摘要翻译： 用于处理固定到多孔基材的样品的方法和系统通常包括限定一个或多个流体隔离区域的压缩机，用于多孔基材的支撑体，其具有对应于压缩机的一个或多个流体隔离区域的开口， 致动器，其使至少一部分压缩机压靠多孔基底，至少当压缩机被压靠在多孔基底上时，流体入口至少进入流体隔离区域，以及流体出口以接收流体，通过开口 在对应于压缩机的流体隔离区域的支撑件中，至少当压缩机被压靠在多孔基底上时。
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公开(公告)号：US20140055853A1
公开(公告)日：2014-02-27
申请号：US13595175
申请日：2012-08-27
CPC分类号： G01N1/31 , B01L3/502715 , B01L3/508 , B01L9/52 , B01L9/527 , B01L2300/041 , B01L2300/046 , B01L2300/0822 , B01L2300/0877 , G01N1/312 , G01N2001/317
摘要： An open top microfluidic device comprising a microfluidic slide carrier and one or more multiplexing stations is provided which allows sequential staining and imaging without the need for using or removing a coverslip on a mounted biological sample.
摘要翻译： 提供了包括微流体载玻片载体和一个或多个多路复用站的开口顶部微流体装置，其允许顺序染色和成像，而不需要在安装的生物样品上使用或去除盖玻片。
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公开(公告)号：US20130287645A1
公开(公告)日：2013-10-31
申请号：US13458092
申请日：2012-04-27
CPC分类号： B32B37/0076 , B01L3/5027 , B01L3/502707 , B01L9/527 , B01L2200/027 , B01L2200/0689 , B01L2300/0816 , B01L2300/0822 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2400/0655 , B32B37/182 , B32B38/0008 , B32B2535/00 , Y10T156/10 , Y10T156/1056 , Y10T156/108
摘要： A microfluidic flow cell subassembly, which may be assembled into a flow cell having fluidic connections outside of the main substrate, is described for encapsulating a sample to allow for subsequent controlled delivery of reagents to the sample, such as multiplexed in situ biomarker staining and analysis. Methods for fabricating the subassembly and assembled flow cell are also provided. The method includes the steps of adhering a gasket layer to a substrate layer, wherein the gasket layer may contain enclosed features and adhering an adherent layer to the gasket layer. The subassembly may be sealed against a solid support to form a flow cell.
摘要翻译： 描述了可以组装成具有在主底物外部的流体连接的流动池的微流体流动池子组件，用于封装样品以允许随后控制的试剂递送到样品中，例如原位复合生物标记染色和分析 。 还提供了用于制造子组件和组装流动池的方法。 该方法包括以下步骤：将衬垫层粘附到衬底层，其中衬垫层可以包含封闭特征并将粘附层粘附到衬垫层。 子组件可以密封在固体支撑件上以形成流动池。
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公开(公告)号：US08900529B2
公开(公告)日：2014-12-02
申请号：US13458092
申请日：2012-04-27
IPC分类号： B01L99/00
CPC分类号： B32B37/0076 , B01L3/5027 , B01L3/502707 , B01L9/527 , B01L2200/027 , B01L2200/0689 , B01L2300/0816 , B01L2300/0822 , B01L2300/0867 , B01L2300/0877 , B01L2300/0887 , B01L2400/0655 , B32B37/182 , B32B38/0008 , B32B2535/00 , Y10T156/10 , Y10T156/1056 , Y10T156/108
摘要： A microfluidic flow cell subassembly, which may be assembled into a flow cell having fluidic connections outside of the main substrate, is described for encapsulating a sample to allow for subsequent controlled delivery of reagents to the sample, such as multiplexed in situ biomarker staining and analysis. Methods for fabricating the subassembly and assembled flow cell are also provided. The method includes the steps of adhering a gasket layer to a substrate layer, wherein the gasket layer may contain enclosed features and adhering an adherent layer to the gasket layer. The subassembly may be sealed against a solid support to form a flow cell.
摘要翻译： 描述了可以组装成具有在主底物外部的流体连接的流动池的微流体流动池子组件，用于封装样品以允许随后控制的试剂递送到样品中，例如原位复合生物标记染色和分析 。 还提供了用于制造子组件和组装流动池的方法。 该方法包括以下步骤：将衬垫层粘附到衬底层，其中衬垫层可以包含封闭特征并将粘附层粘附到衬垫层。 子组件可以密封在固体支撑件上以形成流动池。
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6.发明申请公开(公告)号：US20140039177A1
公开(公告)日：2014-02-06
申请号：US13599124
申请日：2012-08-30
申请人： John Richard Nelson , Li Zhu , Erin Jean Finehout , Xiaohui Chen , Kashan Ali Shaikh , Christopher Michael Puleo , Patrick McCoy Spooner
发明人： John Richard Nelson , Li Zhu , Erin Jean Finehout , Xiaohui Chen , Kashan Ali Shaikh , Christopher Michael Puleo , Patrick McCoy Spooner
IPC分类号： C07H21/00
CPC分类号： G01N27/44791 , C07H1/08 , C07H21/00 , C12N15/1017 , G01N27/44747
摘要： A method of isolating nucleic acids from a biological material, comprises applying the biological material on a substrate comprising one or more cell lysis reagents impregnated therein; applying a fluid to the biological material applied on the substrate; extracting the nucleic acids from the biological material applied on the substrate; and collecting the extracted nucleic acids in a substantially intact form, wherein the collected nucleic acid has a molecular weight greater than or equal to 20 kb.
摘要翻译： 从生物材料中分离核酸的方法包括将生物材料应用于包含其中浸渍的一种或多种细胞裂解试剂的底物; 将液体施加到施加在基底上的生物材料; 从施加在基底上的生物材料中提取核酸; 并以基本上完整的形式收集所提取的核酸，其中所收集的核酸具有大于或等于20kb的分子量。
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公开(公告)号：US20120135458A1
公开(公告)日：2012-05-31
申请号：US12957203
申请日：2010-11-30
申请人： Alex David Corwin , Robert John Filkins , Jun Xie , Christopher James Sevinsky , Kashan Ali Shaikh
发明人： Alex David Corwin , Robert John Filkins , Jun Xie , Christopher James Sevinsky , Kashan Ali Shaikh
CPC分类号： G01N1/312 , G01N2001/368
摘要： A closed loop automated method for staining of a biological sample is provided. The method comprises providing a biological sample, staining at least a portion of the biological sample by flowing in a reagent, monitoring one or more optical characteristics of the biological sample, and calculating a figure of merit based on at least one of the optical characteristics. An automated device for iterative staining of a biological sample is also provided.
摘要翻译： 提供了一种用于染色生物样品的闭环自动化方法。 该方法包括提供生物样品，通过在试剂中流动来染色生物样品的至少一部分，监测生物样品的一种或多种光学特性，以及基于至少一种光学特性来计算品质因数。 还提供了用于生物样品的迭代染色的自动化装置。
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公开(公告)号：US20110129863A1
公开(公告)日：2011-06-02
申请号：US12629088
申请日：2009-12-02
申请人： Philip Alexander Shoemaker , Weston Blaine Griffin , Erin Jean Finehout , Xuefeng Wang , Kashan Ali Shaikh , Greg Darryl Goddard
发明人： Philip Alexander Shoemaker , Weston Blaine Griffin , Erin Jean Finehout , Xuefeng Wang , Kashan Ali Shaikh , Greg Darryl Goddard
CPC分类号： G01N1/4055 , B01L3/5025 , B01L3/50255 , B01L2200/025 , B01L2200/0689 , B01L2300/0627 , B01L2300/0829 , B01L2300/0832 , G01N1/34 , G01N1/405 , G01N1/4077 , G01N30/7233 , G01N35/00029 , G01N35/1095 , G01N2001/4061 , G01N2001/4088 , G01N2030/009 , G01N2030/062 , G01N2035/00128 , Y10T436/24 , Y10T436/25 , Y10T436/25375 , Y10T436/255
摘要： Methods and systems for processing samples fixed to a porous substrate generally comprising, a compressor defining one or more fluid isolation areas, a support, for the porous substrate, having an opening corresponding to one or more of the fluid isolation areas of the compressor, an actuator that causes at least a portion of the compressor to press against the porous substrate, a fluid inlet having access to the fluid isolation area at least when the compressor is pressed against the porous substrate, and a fluid outlet to receive fluid, through the opening in the support corresponding to the fluid isolation area of the compressor, at least when the compressor is pressed against the porous substrate.
摘要翻译： 用于处理固定到多孔基材的样品的方法和系统通常包括限定一个或多个流体隔离区域的压缩机，用于多孔基材的支撑体，其具有对应于压缩机的一个或多个流体隔离区域的开口， 致动器，其使至少一部分压缩机压靠多孔基底，至少当压缩机被压靠在多孔基底上时，流体入口至少进入流体隔离区域，以及流体出口以接收流体，通过开口 在对应于压缩机的流体隔离区域的支撑件中，至少当压缩机被压靠在多孔基底上时。
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公开(公告)号：US09164015B2
公开(公告)日：2015-10-20
申请号：US13538898
申请日：2012-06-29
IPC分类号： H01L27/146 , G01N1/31
CPC分类号： G01N1/312
摘要： A method for processing and imaging a first and second plurality of samples, comprising processing at least one sample from the first plurality of samples, imaging the at least one sample from the first plurality of samples, while being capable of simultaneously processing at least one sample from the second plurality of samples; and imaging the at least one processed sample from the second plurality of samples.
摘要翻译： 一种用于处理和成像第一和第二多个样品的方法，包括处理来自所述第一多个样品的至少一个样品，对来自所述第一多个样品的所述至少一个样品进行成像，同时能够同时处理至少一个样品 从第二多个样本; 以及对来自所述第二多个样本的所述至少一个处理的样品进行成像。
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公开(公告)号：US08673643B2
公开(公告)日：2014-03-18
申请号：US12957203
申请日：2010-11-30
申请人： Alex David Corwin , Robert John Filkins , Jun Xie , Christopher James Sevinsky , Kashan Ali Shaikh
发明人： Alex David Corwin , Robert John Filkins , Jun Xie , Christopher James Sevinsky , Kashan Ali Shaikh
CPC分类号： G01N1/312 , G01N2001/368
摘要： A closed loop automated method for staining of a biological sample is provided. The method comprises providing a biological sample, staining at least a portion of the biological sample by flowing in a reagent, monitoring one or more optical characteristics of the biological sample, and calculating a figure of merit based on at least one of the optical characteristics. An automated device for iterative staining of a biological sample is also provided.
摘要翻译： 提供了一种用于染色生物样品的闭环自动化方法。 该方法包括提供生物样品，通过在试剂中流动来染色生物样品的至少一部分，监测生物样品的一种或多种光学特性，以及基于至少一种光学特性来计算品质因数。 还提供了用于生物样品的迭代染色的自动装置。
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