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70 条记录 (耗时 0.034 秒)

    Active site modified protease .alpha.-1-antitrypsin inhibitors
    1.
    发明授权
    Active site modified protease .alpha.-1-antitrypsin inhibitors 失效
    活性位点修饰的蛋白酶α-1抗胰蛋白酶抑制剂

    公开(公告)号：US4732973A

    公开(公告)日：1988-03-22

    申请号：US620408

    申请日：1984-06-14

    申请人： Philip J. Barr ,  Robert A. Hallewell ,  Steven Rosenberg

    发明人： Philip J. Barr ,  Robert A. Hallewell ,  Steven Rosenberg

    IPC分类号： C07K14/81 ,  C12N15/81 ,  C07K13/00

    CPC分类号： C12N15/81 ,  C07K14/8125 ,  Y10S930/32

    摘要： Proteinaceous composition are provided which inhibit naturally occurring serine proteases. Particularly, an amino acid sequence analogous to human .alpha..sub.1 -antitrypsin is modified at the active site while maintaining protease inhibition. The methionine at the active site is substituted with an oxidatively stable amino acid, while other amino acids may also be changed, added or deleted, particularly at the termini.The yeast strains AB103.1 (pCl/PH05ATi(Val) and AB110 (pCl/1GAPATi(Val) were deposited at the A.T.C.C. on June 18, 1984 and given Accession Nos. 20711 and 20712, respectively.

    摘要翻译： 提供抑制天然存在的丝氨酸蛋白酶的蛋白质组合物。 特别地，类似于人α1-抗胰蛋白酶的氨基酸序列在活性位点被修饰，同时保持蛋白酶抑制。 活性位点处的甲硫氨酸被氧化稳定的氨基酸取代，而其它氨基酸也可以改变，添加或缺失，特别是在末端。 酵母菌株AB103.1（pCl / PH05ATi（Val）和AB110（pCl / 1GAPATi（Val））分别于1984年6月18日在A.T.C.C.保藏，分别登录号20711和20712。

    Eukaryotic regulatable transcription
    2.
    发明授权
    Eukaryotic regulatable transcription 失效
    真核调控转录

    公开(公告)号：US4880734A

    公开(公告)日：1989-11-14

    申请号：US868639

    申请日：1986-05-29

    申请人： Rae L. Burke ,  Steven Rosenberg ,  Jeffrey R. Shuster ,  Patricia Tekamp-Olson ,  Pablo D. T. Valenzuela ,  Philip J. Barr

    发明人： Rae L. Burke ,  Steven Rosenberg ,  Jeffrey R. Shuster ,  Patricia Tekamp-Olson ,  Pablo D. T. Valenzuela ,  Philip J. Barr

    IPC分类号： C07K14/02 ,  C07K14/81 ,  C12N9/02 ,  C12N15/62 ,  C12N15/81

    CPC分类号： C07K14/005 ,  C07K14/8125 ,  C12N15/62 ,  C12N15/81 ,  C12N9/0089 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/40 ,  C07K2319/75 ,  C12N2730/10122

    摘要： Methods and compositions are provided for efficient expression of genes in unicellular microoorganisms, particularly yeast. The systems involve an expression system employing transcriptional initiation regions from glycolytic enzymes, particularly a chimeric expression system, having a first region providing for regulatable or constitutive expression, a second region providing for transcriptional initiation, where regions one and two are not found joined together in functional relationship in nature, and optionally a sequence providing for a secretory leader and processing signal, where the expression cassette will be joined to a gene which may be homologous or heterologous to the host. The expression cassette can be used on an extrachromosomal element or integrated into the host genome, whereby continuous expression can be achieved or inducible expression is obtained, by virtue of the presence or absence of an inducer. Constructions may be provided, where structural genes may be introduced in reading frame with the cassette to provide for expression of the natural peptide, a fused peptide, or a peptide precursor.The S. cerevisiae strain AB110 (pAB24/P. vivax 1-5 was deposited at the A.T.C.C. on May 9, 1986, and given Accession No. 20797.

    摘要翻译： 提供了用于在单细胞微生物，特别是酵母中有效表达基因的方法和组合物。 该系统涉及使用来自糖酵解酶，特别是嵌合表达系统的转录起始区的表达系统，其具有提供可调节或组成型表达的第一区域，提供转录起始的第二区域，其中未发现第一和第二区域连接在一起 功能性关系，以及任选地提供分泌前导序列和处理信号的序列，其中表达盒将与可与宿主同源或异源的基因连接。 表达盒可以在染色体外元件上使用或整合到宿主基因组中，由此可以通过诱导剂的存在或不存在来实现连续表达或获得诱导型表达。 可以提供构建体，其中结构基因可以在盒子的阅读框中引入以提供天然肽，融合肽或肽前体的表达。 酿酒酵母菌株AB110（pAB24 / P.vivax 1-5）于1986年5月9日保藏于A.T.C.C.，保藏号为20797。

    Bak binding protein, DNA encoding the protein, and methods of use thereof
    4.
    发明授权
    Bak binding protein, DNA encoding the protein, and methods of use thereof 有权
    Bak结合蛋白，编码蛋白的DNA及其使用方法

    公开(公告)号：US07316915B2

    公开(公告)日：2008-01-08

    申请号：US10189294

    申请日：2002-07-01

    申请人： Michael C. Kiefer ,  Paul A. Fitzpatrick ,  Helen L. Gibson ,  Philip J. Barr

    发明人： Michael C. Kiefer ,  Paul A. Fitzpatrick ,  Helen L. Gibson ,  Philip J. Barr

    IPC分类号： C12P21/06

    CPC分类号： C07K14/47 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/00 ,  G01N2333/47 ,  G01N2500/02 ,  G01N2510/00

    摘要： The present invention provides polynucleotide sequences (bbp) encoding a Bak Binding Protein (BBP) and fragments thereof that bind to Bak. The invention also provides a BBP which binds to Bak. The invention also provides recombinant host cells containing polynucleotides encoding BBP. The invention further provides antibodies that specifically bind to BBP. The invention further provides methods for detecting agents such as drugs that alter the binding of a BBP with a Bak protein. The invention further provides methods for detecting the presence of bbp or BBP in a biological sample, and further provides methods for modulating the levels of BBP in a cell. This invention additionally encompasses novel peptides, designated the “BBP Binding Domains” and the respective nucleotides, designated “bbpbd-1” and “bbpbd-2” which are involved in the interaction between Bak and BBP.

    摘要翻译： 本发明提供编码Bak结合蛋白（BBP）的多核苷酸序列（bbp）及其与Bak结合的片段。 本发明还提供了结合Bak的BBP。 本发明还提供含有编码BBP的多核苷酸的重组宿主细胞。 本发明还提供了特异性结合BBP的抗体。 本发明还提供了用于检测诸如改变BBP与Bak蛋白的结合的药物的药物的方法。 本发明还提供了用于检测生物样品中bbp或BBP的存在的方法，并且还提供了调节细胞中BBP水平的方法。 本发明另外包括称为“BBP结合域”的新肽，以及涉及Bak和BBP之间的相互作用的“bbpbd-1”和“bbpbd-2”的相应核苷酸。

    Bak binding protein, DNA encoding the protein, and methods of use thereof
    5.
    发明授权
    Bak binding protein, DNA encoding the protein, and methods of use thereof 有权
    Bak结合蛋白，编码蛋白的DNA及其使用方法

    公开(公告)号：US06441135B1

    公开(公告)日：2002-08-27

    申请号：US09381488

    申请日：2000-02-11

    申请人： Michael C. Kiefer ,  Paul A. Fitzpatrick ,  Helen L. Gibson ,  Philip J. Barr

    发明人： Michael C. Kiefer ,  Paul A. Fitzpatrick ,  Helen L. Gibson ,  Philip J. Barr

    IPC分类号： C07K100

    CPC分类号： C07K14/47 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/00 ,  G01N2333/47 ,  G01N2500/02 ,  G01N2510/00

    摘要： The present invention provides polynucleotide sequences (bbp) encoding a Bak Binding Protein (BBP) and fragments thereof that bind to Bak. The invention also provides a BBP which binds to Bak. The invention also provides recombinant host cells containing polynucleotides encoding BBP. The invention further provides antibodies that specifically bind to BBP. The invention further provides methods for detecting agents such as drugs that alter the binding of a BBP with a Bak protein. The invention neither provides methods for detecting the presence of bbp or BBP in a biological sample, and further provides methods for modulating the levels of BBP in a cell. This invention additionally encompasses novel peptides, designated the “BBP Binding Domains” and the respective nucleotides, designated “bbpbd-1” and “bbpbd-2” which arc involved in the interaction between Bak and BBP.

    摘要翻译： 本发明提供编码Bak结合蛋白（BBP）的多核苷酸序列（bbp）及其与Bak结合的片段。 本发明还提供了结合Bak的BBP。 本发明还提供含有编码BBP的多核苷酸的重组宿主细胞。 本发明还提供了特异性结合BBP的抗体。 本发明还提供了用于检测诸如改变BBP与Bak蛋白的结合的药物的药物的方法。 本发明既没有提供用于检测生物样品中bbp或BBP的存在的方法，并且还提供了调节细胞中BBP水平的方法。 本发明还包括命名为“BBP结合域”的新肽，以及涉及Bak和BBP之间相互作用的“bbpbd-1”和“bbpbd-2”的相应核苷酸。

    Lysine-specific Porphyromonas gingivalis proteinase
    9.
    发明授权
    Lysine-specific Porphyromonas gingivalis proteinase 失效
    赖氨酸特异性牙龈卟啉单胞菌牙龈蛋白酶

    公开(公告)号：US5475097A

    公开(公告)日：1995-12-12

    申请号：US141324

    申请日：1993-10-21

    申请人： James Travis ,  J. Stanislaw Potempa ,  Philip J. Barr ,  Nadine Pavloff ,  Robert N. Pike

    发明人： James Travis ,  J. Stanislaw Potempa ,  Philip J. Barr ,  Nadine Pavloff ,  Robert N. Pike

    IPC分类号： C12N9/52 ,  C12N15/51

    CPC分类号： C12N9/52

    摘要： Provide herein is a substantially pure Lys-gingipain complex preparation, Lys-gingipain being characterized as having an apparent molecular mass of 105 kDa as estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, where sample is prepared without boiling, said Lys-gingipain having amidolytic and proteolytic activity for cleavage after lysine residues and having no amidolytic and/or proteolytic activity for cleavage after arginine residues, wherein the amidolytic and/or proteolytic activity is inhibited by TLCK, cysteine protease group-specific inhibitors including iodoacetamide and iodoacetic acid, wherein the amidolytic and/or proteolytic activity of said Lys-gingipain is not sensitive to inhibition by leupeptin, antipain, trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane, serine protease group-specific inhibitors including diisopropylfluorophosphate and phenylmethyl sulfonylfluoride, and antibodies specific for the Lys-gingipain protein complex and its catalytic component, methods for preparation. As specifically exemplified, a Lys-gingipain protein complex is purified from Porphyromonas gingivalis H66, and the 60 kDa catalytic component of the Lys-gingipain protein complex has an amino acid sequence as given in SEQ ID NO: 14 from amino acid 1 through amino acid 509. Also provided are nucleic acid sequences encoding this catalytic protein. The nucleotide coding sequence of the 60 kDa catalytic component of the Lys-gingipain protein complex is given in SEQ ID NO:13, from nucleotide 1336 through nucleotide 2863. The Lys-gingipain complex also comprises a hemagglutinin component identified by an N-terminal amino acid sequence as given in SEQ ID NO:14, amino acids 510-714.

    摘要翻译： 本文提供了一种基本上纯的赖氨酸蛋白糊精复合物制剂，其特征在于具有105kDa的表观分子量，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定，其中样品在不沸腾的情况下制备，所述Lys-盖蛋白具有酰胺分解和 在赖氨酸残基之后切割的蛋白水解活性，并且在精氨酸残基之后没有用于切割的酰胺解和/或蛋白水解活性，其中所述酰胺分解和/或蛋白水解活性被TLCK抑制，包括碘乙酰胺和碘乙酸的半胱氨酸蛋白酶组特异性抑制剂，其中酰胺分解 所述Lys-gingipain的蛋白水解和/或蛋白水解活性对亮抑酶肽，抗胰蛋白酶，反式环氧琥珀酰基-L-亮氨酰氨基 - （4-胍基）丁烷，丝氨酸蛋白酶组特异性抑制剂，包括二异丙基氟磷酸酯和苯基甲基磺酰氟的抑制和抗体特异性 用于Lys-gingipain蛋白复合物及其催化 组分，制备方法。 如具体示例的那样，从牙龈卟啉单胞菌H66中纯化Lys-gingipain蛋白复合物，Lys-gingipain蛋白复合物的60kDa催化组分具有SEQ ID NO：14中从氨基酸1到氨基酸所给出的氨基酸序列 还提供了编码该催化蛋白的核酸序列。 Lys-gingipain蛋白质复合物的60kDa催化组分的核苷酸编码序列在SEQ ID NO：13中由核苷酸1336至核苷酸2863给出。Lys-gingipain复合物还包含由N-末端氨基酸鉴定的血凝素成分 酸序列，如SEQ ID NO：14所示，氨基酸510-714。