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    Process for Production of Beer or Beer-Like Beverage
    4.
    发明申请
    Process for Production of Beer or Beer-Like Beverage 审中-公开
    生产啤酒或啤酒样饮料的方法

    公开(公告)号：US20090123598A1

    公开(公告)日：2009-05-14

    申请号：US11988130

    申请日：2006-06-05

    申请人： Hitoshi Amano ,  Shotaro Yamaguchi

    发明人： Hitoshi Amano ,  Shotaro Yamaguchi

    IPC分类号： C12H1/15 ,  C12C11/00

    CPC分类号： C12C5/004 ,  C12C7/04

    摘要： Disclosed is a process for producing a beer or beer-like beverage having mellowness, a special flavor and a good taste. The beer or beer-like beverage can be produced by allowing a protein deamidase to act on at least a part of a raw material during the production process.

    摘要翻译： 公开了一种生产具有醇厚，特殊风味和良好味道的啤酒或啤酒状饮料的方法。 可以通过在生产过程中使蛋白质脱酰胺酶作用于原料的至少一部分来生产啤酒或啤酒状饮料。

    TANNASE, GENE ENCODING SAME, AND PROCESS FOR PRODUCING SAME
    7.
    发明申请
    TANNASE, GENE ENCODING SAME, AND PROCESS FOR PRODUCING SAME 有权
    TANNASE，编码它们的基因及其生产方法

    公开(公告)号：US20110195155A1

    公开(公告)日：2011-08-11

    申请号：US13124510

    申请日：2009-10-02

    申请人： Megumi Nakagawa ,  Naoki Matsumoto ,  Hitoshi Amano ,  Shotaro Yamaguchi

    发明人： Megumi Nakagawa ,  Naoki Matsumoto ,  Hitoshi Amano ,  Shotaro Yamaguchi

    IPC分类号： C12N15/80 ,  C12N9/18 ,  C12N1/15 ,  C07H21/04 ,  A23L2/00

    CPC分类号： C12N9/18 ,  C12P7/42

    摘要： Disclosed is a thermostable tannase derived from a microorganism. Specifically disclosed is a thermostable tannase derived from Aspergillus awamori or Aspergillus niger. A preferred embodiment of the tannase has the following chemoenzymatic properties: (1) activity: to act on a depside bond to thereby cause hydrolysis; (2) molecular weight: about 230,000 Da (as measured by gel filtration); and (3) thermal stability: stable at a temperature up to 65° C. (pH 5.0, 30 min.)

    摘要翻译： 公开了一种衍生自微生物的热稳定性鞣酸酶。 具体公开了源自泡盛曲霉或黑曲霉的热稳定性鞣酸酶。 鞣酸酶的优选实施方案具有以下化学酶学性质：（1）活性：作用于脱附键从而引起水解; （2）分子量：约230,000Da（通过凝胶过滤测量）; 和（3）热稳定性：在高达65℃的温度下稳定（pH5.0,30分钟）

    STABILIZED TRANSGLUTAMINASE AND PROCESS FOR PRODUCTION THEREOF
    8.
    发明申请
    STABILIZED TRANSGLUTAMINASE AND PROCESS FOR PRODUCTION THEREOF 审中-公开
    稳定的转谷氨酰胺酶及其生产方法

    公开(公告)号：US20100297726A1

    公开(公告)日：2010-11-25

    申请号：US12864025

    申请日：2009-01-22

    申请人： Masamichi Okada ,  Yoshiaki Kurono ,  Hitoshi Amano ,  Shotaro Yamaguchi

    发明人： Masamichi Okada ,  Yoshiaki Kurono ,  Hitoshi Amano ,  Shotaro Yamaguchi

    IPC分类号： C12N9/96 ,  C12N9/10

    CPC分类号： C12N9/1044 ,  C12N9/96 ,  C12Y203/02013

    摘要： Disclosed is a transglutaminase having excellent stability. Also disclosed is a process for producing the transglutaminase. Specifically disclosed is a stabilized transglutaminase, which has such a structure in which a pro-sequence peptide of transglutaminase is bound to a mature transglutaminase. Also specifically disclosed is a process for producing stabilized transglutaminase, which includes the steps of: culturing a microorganism capable of producing transglutaminase under the conditions where transglutaminase can be produced; and separating and collecting matured transglutaminase having a pro-sequence peptide bound thereto from a culture medium.

    摘要翻译： 公开了具有优异稳定性的转谷氨酰胺酶。 还公开了用于生产转谷氨酰胺酶的方法。 具体公开了一种稳定的转谷氨酰胺酶，其具有转谷氨酰胺酶的前序肽与成熟转谷氨酰胺酶结合的结构。 还具体公开了一种生产稳定的转谷氨酰胺酶的方法，其包括以下步骤：在可产生转谷氨酰胺酶的条件下培养能够产生转谷氨酰胺酶的微生物; 并从培养基中分离并收集与其结合的前序肽的成熟转谷氨酰胺酶。

    Method for designing mutant protein deamidase
    9.
    发明授权
    Method for designing mutant protein deamidase 有权
    设计突变蛋白脱酰胺酶的方法

    公开(公告)号：US08735131B2

    公开(公告)日：2014-05-27

    申请号：US13062798

    申请日：2009-08-12

    申请人： Ryota Hashizume ,  Bunzo Mikami ,  Hirotaka Matsubara ,  Akiko Matsunaga ,  Shotaro Yamaguchi

    发明人： Ryota Hashizume ,  Bunzo Mikami ,  Hirotaka Matsubara ,  Akiko Matsunaga ,  Shotaro Yamaguchi

    IPC分类号： C12P21/00 ,  C12N15/00 ,  C12N9/78

    CPC分类号： C12N9/80 ,  C12N9/48

    摘要： An object is to provide a novel method for improving an enzyme capable of deamidating a protein. A mutant protein deamidase is designed by the following steps: (A) identifying one or more amino acid in an amino acid sequence for a protein deamidase which corresponds to the amino acid at position 35, 38 to 43, 45, 46, 49, 79 to 84, 103 to 106, 117, 142, 143, 146, 166, or 185 in the amino acid sequence set forth in SEQ ID NO: 2; and (B) constructing a mutant amino acid sequence of the protein deamidase by substituting the one or more amino acid identified in step (A) with another amino acid or other amino acids or by deleting the one or more amino acid identified in step (A).

    摘要翻译： 目的是提供一种改善能够使蛋白质脱酰胺化的酶的新方法。 通过以下步骤设计突变型蛋白质脱酰胺酶：（A）鉴定氨基酸序列中一个或多个对应于第35,38,43,45,46,49,79位的氨基酸的蛋白质脱酰胺酶的氨基酸 至SEQ ID NO：2所示氨基酸序列中的84,103至106,117,142,143,146,166或185; 和（B）通过用步骤（A）中鉴定的一个或多个氨基酸用另一个氨基酸或其他氨基酸取代蛋白质脱酰胺酶的突变氨基酸序列，或通过删除步骤（A）中鉴定的一个或多个氨基酸 ）。