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公开(公告)号：US20100159513A1
公开(公告)日：2010-06-24
申请号：US12619790
申请日：2009-11-17
CPC分类号： C12P21/02
摘要： Several endogenous genes have been identified in Escherichia coli, the overexpression of which increases recombinant peptide production. Increasing the copy number of aroB, aroK, proB, or crl increases the amount of a recombinant peptide produced by a host cell. Recombinant host cells comprising at least one chimeric genetic construct encoding a peptide of interest and at least one genetic modification that increases recombinant peptide production are provided as well as methods of using such recombinant host cells
摘要翻译： 已经在大肠杆菌中鉴定了几种内源基因，其过表达增加了重组肽的产生。 增加aroB，aroK，proB或crl的拷贝数增加了由宿主细胞产生的重组肽的量。 提供了包含至少一种编码目的肽的嵌合基因构建体和至少一种提高重组肽产生的遗传修饰的重组宿主细胞以及使用这种重组宿主细胞的方法
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2.发明申请USE OF TETRACYSTEINE TAGS IN FLUORESCENCE-ACTIVATED CELL SORTING ANALYSIS OF PROKARYOTIC CELLS PRODUCING PEPTIDES OR PROTEINS 失效在生产肽或蛋白质的原核细胞的荧光激活细胞分选中使用四肽蛋白标签公开(公告)号：US20090117609A1
公开(公告)日：2009-05-07
申请号：US12263608
申请日：2008-11-03
申请人： Qiong Cheng , Kevin Michael Croker , Stephen R. Fahnestock , Tanja Maria Gruber , Kristin Ruebling-Jass , Jianzhong Zhang
发明人： Qiong Cheng , Kevin Michael Croker , Stephen R. Fahnestock , Tanja Maria Gruber , Kristin Ruebling-Jass , Jianzhong Zhang
CPC分类号： C12N15/62 , C07K2319/20 , C07K2319/60
摘要： A process of in vivo labeling and identifying recombinantly produced peptides or proteins within an unpermeabilized prokaryotic host cell. Recombinant prokaryotic cells expressing a fusion peptide comprising at least one tetracysteine tag were labeled in vivo using a biarsenical labeling reagent. A fluorescent activated cell sorter was used to identify and select subpopulations of fluorescent cells wherein the amount of fusion peptide in the cell was proportional to the amount of fluorescence detected.
摘要翻译： 体内标记和鉴定重组产生的肽或蛋白质在未经过预处理的原核宿主细胞内的过程。 表达含有至少一个四羧酸标签的融合肽的重组原核细胞在体内用双标记标记试剂进行标记。 使用荧光活化细胞分选器来鉴定和选择荧光细胞亚群，其中细胞中融合肽的量与所检测的荧光量成比例。
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3.发明授权Use of tetracysteine tags in fluorescence-activated cell sorting analysis of prokaryotic cells producing peptides or proteins 失效在用于产生肽或蛋白质的原核细胞的荧光激活细胞分选分析中使用四羧酸标签公开(公告)号：US07794963B2
公开(公告)日：2010-09-14
申请号：US12263608
申请日：2008-11-03
申请人： Qiong Cheng , Kevin Michael Croker , Stephen R. Fahnestock , Tanja Maria Gruber , Kristin Ruebling-Jass , Jianzhong Zhang
发明人： Qiong Cheng , Kevin Michael Croker , Stephen R. Fahnestock , Tanja Maria Gruber , Kristin Ruebling-Jass , Jianzhong Zhang
IPC分类号： G01N33/569 , C07K16/00
CPC分类号： C12N15/62 , C07K2319/20 , C07K2319/60
摘要： A process of in vivo labeling and identifying recombinantly produced peptides or proteins within an unpermeabilized prokaryotic host cell. Recombinant prokaryotic cells expressing a fusion peptide comprising at least one tetracysteine tag were labeled in vivo using a biarsenical labeling reagent. A fluorescent activated cell sorter was used to identify and select subpopulations of fluorescent cells wherein the amount of fusion peptide in the cell was proportional to the amount of fluorescence detected.
摘要翻译： 体内标记和鉴定重组产生的肽或蛋白质在未经过预处理的原核宿主细胞内的过程。 表达含有至少一个四羧酸标签的融合肽的重组原核细胞在体内用双标记标记试剂进行标记。 使用荧光活化细胞分选器来鉴定和选择荧光细胞亚群，其中细胞中融合肽的量与所检测的荧光量成比例。
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公开(公告)号：US07662587B1
公开(公告)日：2010-02-16
申请号：US12398358
申请日：2009-03-05
CPC分类号： C12N15/70 , C07K14/245 , C07K2319/00 , C07K2319/50
摘要： Disrupting the expression of endogenous Escherichia host cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production relative to control Escherichia host cells. Recombinant Escherichia host cells are provided as well as methods of using such host cells for heterologous peptide production.
摘要翻译： 破坏内源性大肠杆菌宿主细胞基因gcvA和spr的表达提供相对于对照大肠杆菌宿主细胞具有增加的异源肽产生的突变宿主细胞。 提供重组埃希氏杆菌宿主细胞以及使用这种宿主细胞进行异源肽生产的方法。
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5.发明授权Host cell modifications that improve peptide production and downstream processing 失效改善肽生产和下游加工的宿主细胞修饰公开(公告)号：US07915011B2
公开(公告)日：2011-03-29
申请号：US12575550
申请日：2009-10-08
CPC分类号： C07K14/245 , C07K2319/00 , C07K2319/50 , C12N15/70
摘要： Disrupting the expression of endogenous Escherichia host cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production. The addition of a genetic modification to the coding region of gene yejM further enhances peptide production and facilitates easier downstream processing. Recombinant Escherichia host cells are provided as well as methods of using such host cells for heterologous peptide production.
摘要翻译： 破坏内源性大肠杆菌宿主细胞基因gcvA和spr的表达提供具有增加的异源肽产生的突变宿主细胞。 在基因yejM的编码区添加遗传修饰进一步增强了肽的产生，并且便于下游处理。 提供重组埃希氏杆菌宿主细胞以及使用这种宿主细胞进行异源肽生产的方法。
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6.发明申请公开(公告)号：US20100227361A1
公开(公告)日：2010-09-09
申请号：US12575550
申请日：2009-10-08
CPC分类号： C07K14/245 , C07K2319/00 , C07K2319/50 , C12N15/70
摘要： Disrupting the expression of endogenous Escherichia host cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production. The addition of a genetic modification to the coding region of gene yejM further enhances peptide production and facilitates easier downstream processing. Recombinant Escherichia host cells are provided as well as methods of using such host cells for heterologous peptide production.
摘要翻译： 破坏内源性大肠杆菌宿主细胞基因gcvA和spr的表达提供具有增加的异源肽产生的突变宿主细胞。 在基因yejM的编码区添加遗传修饰进一步增强了肽的产生，并且便于下游处理。 提供重组埃希氏杆菌宿主细胞以及使用这种宿主细胞进行异源肽生产的方法。
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公开(公告)号：US08673602B2
公开(公告)日：2014-03-18
申请号：US13210550
申请日：2011-08-16
申请人： Qi Chen , Qiong Cheng , Jian Ping Lai , Kristin Ruebling-Jass
发明人： Qi Chen , Qiong Cheng , Jian Ping Lai , Kristin Ruebling-Jass
CPC分类号： C12P7/18 , C07K14/245 , C12N9/1051 , C12N9/1205 , C12N9/2431 , C12P7/20 , C12P7/42
摘要： Recombinant bacteria having an improved ability to utilize sucrose are provided. These recombinant bacteria have nucleotide sequences encoding sucrose utilization polypeptides integrated into their genome between the yihP gene or its homolog and the yihO gene or its homolog. Additionally, methods of utilizing the recombinant bacteria to produce products such as glycerol and glycerol-derived products are provided.
摘要翻译： 提供了具有改善的利用蔗糖能力的重组细菌。 这些重组细菌具有编码在yihP基因或其同源物和yihO基因或其同源物之间整合到其基因组中的蔗糖利用多肽的核苷酸序列。 此外，提供了利用重组细菌产生产物如甘油和甘油衍生产物的方法。
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公开(公告)号：US09273336B2
公开(公告)日：2016-03-01
申请号：US13164990
申请日：2011-06-21
申请人： Ritu Bhalla , Qi Chen , Qiong Cheng , Neeraj Pandey , Pierre E. Rouviere , Kristin Ruebling-Jass , Annapurna Sachan
发明人： Ritu Bhalla , Qi Chen , Qiong Cheng , Neeraj Pandey , Pierre E. Rouviere , Kristin Ruebling-Jass , Annapurna Sachan
CPC分类号： C12P21/02 , C12N1/02 , C12N9/1025 , C12N15/70
摘要： Methods are provided to obtain recombinant microbial cells having at least one genetic modification that increase the buoyant density of a recombinant microbial cell or the buoyant density of inclusion bodies produced within a recombinant microbial cell. Exemplified are genetic modifications that increase the buoyant density of a recombinant microbial cell expressing heterologous peptides and polypeptides. Increasing expression of the genes ysaB, glyQ, glyS or a combination thereof within the recombinant microbial cell produces cells or inclusion bodies having higher buoyant density. A similar effect was achieved by decreasing or disrupting expression of the endogenous gltA gene. Increases in buoyant density render peptide production more efficient with respect to time and costs.
摘要翻译： 提供了获得具有增加重组微生物细胞的浮力密度或在重组微生物细胞内产生的包涵体的浮力密度的至少一种遗传修饰的重组微生物细胞的方法。 示例是增加表达异源肽和多肽的重组微生物细胞的浮力密度的遗传修饰。 在重组微生物细胞内增加基因ysaB，glyQ，glyS或其组合的表达产生具有较高浮力密度的细胞或包涵体。 通过减少或破坏内源性gltA基因的表达也可以获得类似的效果。 浮力密度的增加使肽生产在时间和成本方面更有效率。
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9.发明申请VARIANT SUCROSE TRANSPORTER POLYPEPTIDES THAT ENABLE FASTER SUCROSE UTILIZATION IN BACTERIA 失效更多的SUCROSE运输机聚合物，能够在细菌中使用更快的SUCROSE利用公开(公告)号：US20130045518A1
公开(公告)日：2013-02-21
申请号：US13210488
申请日：2011-08-16
申请人： QI Chen , Qiong Cheng , Jian Ping Lai , Kristin Ruebling-Jass
发明人： QI Chen , Qiong Cheng , Jian Ping Lai , Kristin Ruebling-Jass
IPC分类号： C12P7/42 , C12P7/18 , C12P7/20 , C07K14/245 , C12N1/21
CPC分类号： C12P7/42 , C07K14/245 , C12N9/1051 , C12N9/1205 , C12N9/2451 , C12P7/18 , C12P7/20
摘要： Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria are described. Additionally, variant or recombinant bacteria comprising these variant sucrose transporter polypeptides, and methods of utilizing the bacteria to produce products such as glycerol and glycerol-derived products are provided.
摘要翻译： 描述了使细菌中蔗糖利用更快的变体蔗糖转运蛋白多肽。 此外，提供了包含这些变体蔗糖转运蛋白多肽的变体或重组细菌，以及利用细菌产生产物如甘油和甘油衍生产物的方法。
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10.发明申请RECOMBINANT ESCHERICHIA COLI HAVING ENHANCED ACETYL-COENZYME A SYNTHETASE ACTIVITY FOR PRODUCING GLYEROL AND GLYCEROL-DERIVED PRODUCTS 失效具有增强的乙酰胆碱的重组蛋白合成酶用于生产甘油和甘油衍生产物的合成酶活性公开(公告)号：US20120122168A1
公开(公告)日：2012-05-17
申请号：US12946120
申请日：2010-11-15
申请人： QI CHEN , QIONG CHENG , ANDREW C. ELIOT , Kristin Ruebling-Jass
发明人： QI CHEN , QIONG CHENG , ANDREW C. ELIOT , Kristin Ruebling-Jass
摘要： Recombinant E. coli having enhanced acetyl-CoA synthetase activity and the ability to produce glycerol and glycerol-derived products, such as 3-hydroxypropionic acid, methylglyoxal, 1,2-propanediol, and 1,3-propanediol, are described. The recombinant E. coli comprise a promoter operably linked to a nucleotide sequence encoding a polypeptide having acetyl-CoA synthetase enzyme activity, wherein the promoter and nucleotide sequence are each independently either native or non-native.
摘要翻译： 描述了具有增强的乙酰辅酶A合成酶活性和产生甘油和甘油衍生产物如3-羟基丙酸，甲基乙二醛，1,2-丙二醇和1,3-丙二醇的能力的重组大肠杆菌。 重组大肠杆菌包含与编码具有乙酰-CoA合成酶活性的多肽的核苷酸序列可操作地连接的启动子，其中启动子和核苷酸序列各自独立地是天然的或非天然的。
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