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公开(公告)号：US20240272169A1
公开(公告)日：2024-08-15
申请号：US18598736
申请日：2024-03-07
发明人： Brian Reed , Jeremy Lackey , Haidong Huang
IPC分类号： G01N33/58 , C07K14/47 , C07K19/00 , C12Q1/6806 , G01N1/28 , G01N21/64 , G01N33/68 , G16B25/10 , G16B30/00 , G16B40/00 , G16B40/10 , G16B50/00 , G16B50/30
CPC分类号： G01N33/581 , C07K14/47 , C07K19/00 , C12Q1/6806 , G01N1/28 , G01N21/6428 , G01N33/58 , G01N33/582 , G01N33/6821 , G01N33/6824 , G16B25/10 , G16B40/00 , G16B40/10 , G16B50/30 , G01N2021/6439 , G01N2458/00 , G16B30/00 , G16B50/00
摘要： Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.
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公开(公告)号：US12055548B2
公开(公告)日：2024-08-06
申请号：US16709077
申请日：2019-12-10
发明人： Brian Reed , Jeremy Lackey , Haidong Huang
IPC分类号： A61K38/00 , C07K14/47 , C07K19/00 , C12Q1/6806 , G01N1/28 , G01N21/64 , G01N33/58 , G01N33/68 , G16B25/10 , G16B40/00 , G16B40/10 , G16B50/30 , G16B30/00 , G16B50/00
CPC分类号： G01N33/581 , C07K14/47 , C07K19/00 , C12Q1/6806 , G01N1/28 , G01N21/6428 , G01N33/58 , G01N33/582 , G01N33/6821 , G01N33/6824 , G16B25/10 , G16B40/00 , G16B40/10 , G16B50/30 , G01N2021/6439 , G01N2458/00 , G16B30/00 , G16B50/00
摘要： Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.
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公开(公告)号：US20230221253A1
公开(公告)日：2023-07-13
申请号：US18095767
申请日：2023-01-11
发明人： Brian Reed , Todd Rearick , Gerard Schmid , Haidong Huang
CPC分类号： G01N21/6428 , G01N33/6821 , G01N33/6824 , G01N33/582 , G01N1/28 , G01N2021/6439 , G01N2458/00 , G16B30/00
摘要： Techniques for multi-dimensional signal analysis are described herein. The techniques may be used in one or more sequencing applications. For example, according to some aspects, there is provided a method comprising: determining information about a sample that emits emission light in response to excitation light based on at least one of pulse duration and interpulse duration and at least two of wavelength, intensity, and lifetime of the emission light, wherein the sample comprises a reagent configured to be coupled to a luminescent label, and wherein a shielding element is disposed between the reagent and the luminescent label.
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公开(公告)号：US20220324910A1
公开(公告)日：2022-10-13
申请号：US17709917
申请日：2022-03-31
发明人： Omer Ad , Haidong Huang
IPC分类号： C07K7/06 , C07D401/12 , C07K1/02 , C12P21/02
摘要： The present disclosure is related to peptides comprising modified aspartic acid and glutamic acid moieties, methods of making such peptides, and methods of using such modified peptides to selectively direct cleavage of peptide bonds. Selective peptide bond cleavage is advantageous in peptide sequencing applications, such as automated peptide sequencing applications.
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公开(公告)号：US20220073973A1
公开(公告)日：2022-03-10
申请号：US17314626
申请日：2021-05-07
发明人： Jonathan M. Rothberg , Jeremy Lackey , Brian Reed , Xinghua Shi , Haidong Huang
IPC分类号： C12Q1/6818 , C12Q1/6869 , G01N21/64 , C12Q1/6874
摘要： Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.
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公开(公告)号：US20240182950A1
公开(公告)日：2024-06-06
申请号：US18491699
申请日：2023-10-20
发明人： David N. Kamber , Kenneth Skinner , Haidong Huang
IPC分类号： C12Q1/6806 , C12Q1/6834
CPC分类号： C12Q1/6806 , C12Q1/6834
摘要： According to some aspects, systems and methods for characterizing phosphoserine-containing polypeptides are described. In some embodiments, a phosphoserine residue of a polypeptide may be converted to a dehydroalanine residue. Some aspects are directed to a method of reacting a polypeptide comprising at least one dehydroalanine residue with a protected thiol compound comprising a click chemistry handle (e.g., an azide, an alkyne) to provide a functionalized polypeptide comprising a click chemistry handle. Some aspects are directed to systems and methods of characterizing phosphoserine residues in polypeptides (e.g., identifying and/or determining the location of a phosphoserine residue) and/or methods of preparing samples for such systems and methods.
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公开(公告)号：US20240151729A1
公开(公告)日：2024-05-09
申请号：US18491693
申请日：2023-10-20
发明人： Haidong Huang , Saketh Gudipati , Roger R. Nani
CPC分类号： G01N33/582 , G01N33/6803
摘要： Provided herein are luminescently labeled oligonucleotide structures, which may be used in systems and methods for polypeptide sequencing and/or nucleic acid sequencing.
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公开(公告)号：US20220187205A1
公开(公告)日：2022-06-16
申请号：US17551085
申请日：2021-12-14
IPC分类号： G01N21/64 , G01N33/543
摘要： Aspects of the disclosure relate to methods and systems for regenerating a sensor chip surface, including techniques for reuse of a single sensor chip in multiple sampling cycles by regenerating a surface of the sensor chip between successive sampling cycles. A method is provided for reusing an integrated device to process a sample, the sample being divided into a plurality of aliquots, the method comprising: loading a first aliquot of the plurality of aliquots into at least some of a plurality of chambers of the integrated device; sampling analytes of the first aliquot while the analytes are present in the at least some of the plurality of chambers; removing the first aliquot from the at least some of the plurality of chambers of the integrated device; and loading a second aliquot of the plurality of aliquots into the at least some of the plurality of chambers of the integrated device.
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公开(公告)号：US11001875B2
公开(公告)日：2021-05-11
申请号：US16212724
申请日：2018-12-07
发明人： Jonathan M. Rothberg , Jeremy Lackey , Brian Reed , Xinghua Shi , Haidong Huang
IPC分类号： C12Q1/6818 , C12Q1/6874 , G01N21/64 , C12Q1/6869
摘要： Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.
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公开(公告)号：US20190330688A1
公开(公告)日：2019-10-31
申请号：US16212724
申请日：2018-12-07
发明人： Jonathan M. Rothberg , Jeremy Lackey , Brian Reed , Xinghua Shi , Haidong Huang
IPC分类号： C12Q1/6818 , C12Q1/6869 , G01N21/64 , C12Q1/6874
摘要： Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.
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