公开(公告)号：US20240209434A1

公开(公告)日：2024-06-27

申请号：US18431313

申请日：2024-02-02

Applicant: Quantum-Si Incorporated

Inventor： Guojun Chen ,  Jeremy Lackey ,  Alexander Goryaynov ,  Gerard Schmid ,  Ali Kabiri ,  Jonathan M. Rothberg ,  Todd Rearick ,  Jonathan C. Schultz ,  Farshid Ghasemi ,  Keith G. Fife

IPC: C12Q1/6869 ,  B01L3/00 ,  C08L1/02 ,  C12Q1/6806 ,  G01N21/64 ,  G01N27/447 ,  G01N30/86 ,  G01N30/88

CPC classification number: C12Q1/6869 ,  B01L3/502761 ,  B01L3/50857 ,  C08L1/02 ,  C12Q1/6806 ,  G01N21/6428 ,  G01N21/6454 ,  G01N27/44743 ,  G01N27/44782 ,  G01N30/86 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0421 ,  G01N27/447 ,  G01N30/88

Abstract: Apparatus and techniques for electrokinetic loading of samples of interest into sub-micron-scale reaction chambers are described. Embodiments include an integrated device and related apparatus for analyzing samples in parallel. The integrated device may include at least one reaction chamber formed through a surface of the integrated device and configured to receive a sample of interest, such as a molecule of nucleic acid. The integrated device may further include electrodes patterned adjacent to the reaction chamber that produce one or more electric fields that assist loading the sample into the reaction chamber. The apparatus may further include a sample reservoir having a fluid seal with the surface of the integrated device and configured to hold a suspension containing the samples.

公开(公告)号：US20180326412A1

公开(公告)日：2018-11-15

申请号：US15971493

申请日：2018-05-04

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Jeremy Lackey ,  Guojun Chen

IPC: B01L3/00 ,  C12Q1/6848 ,  C09D5/16 ,  C09D171/02 ,  C09D187/00

CPC classification number: B01L3/508 ,  B01L2200/141 ,  B01L2300/165 ,  C09D5/1662 ,  C09D171/02 ,  C09D187/005 ,  C12Q1/6848

Abstract: Methods of preparing surfaces of sample wells are provided. In some aspects, methods of preparing a sample well surface involve contacting the sample well with a block copolymer to form an antifouling overlay over a metal oxide surface of the sample well. In some aspects, methods of passivating and/or selectively functionalizing a sample well surface are provided.

公开(公告)号：US20220187205A1

公开(公告)日：2022-06-16

申请号：US17551085

申请日：2021-12-14

Applicant: Quantum-Si Incorporated

Inventor： Guojun Chen ,  Gerard Schmid ,  Todd Rearick ,  Thomas Raymond Thurston ,  Haidong Huang

IPC: G01N21/64 ,  G01N33/543

Abstract: Aspects of the disclosure relate to methods and systems for regenerating a sensor chip surface, including techniques for reuse of a single sensor chip in multiple sampling cycles by regenerating a surface of the sensor chip between successive sampling cycles. A method is provided for reusing an integrated device to process a sample, the sample being divided into a plurality of aliquots, the method comprising: loading a first aliquot of the plurality of aliquots into at least some of a plurality of chambers of the integrated device; sampling analytes of the first aliquot while the analytes are present in the at least some of the plurality of chambers; removing the first aliquot from the at least some of the plurality of chambers of the integrated device; and loading a second aliquot of the plurality of aliquots into the at least some of the plurality of chambers of the integrated device.

公开(公告)号：US20210310066A1

公开(公告)日：2021-10-07

申请号：US17350509

申请日：2021-06-17

Applicant: Quantum-Si Incorporated

Inventor： Guojun Chen ,  Jeremy Lackey ,  Alexander Goryaynov ,  Gerard Schmid ,  Ali Kabiri ,  Jonathan M. Rothberg ,  Todd Rearick ,  Jonathan C. Schultz ,  Farshid Ghasemi ,  Keith G. Fife

IPC: C12Q1/6869 ,  G01N21/64 ,  B01L3/00 ,  G01N30/86 ,  C08L1/02 ,  C12Q1/6806 ,  G01N27/447

Abstract: Apparatus and techniques for electrokinetic loading of samples of interest into sub-micron-scale reaction chambers are described. Embodiments include an integrated device and related apparatus for analyzing samples in parallel. The integrated device may include at least one reaction chamber formed through a surface of the integrated device and configured to receive a sample of interest, such as a molecule of nucleic acid. The integrated device may further include electrodes patterned adjacent to the reaction chamber that produce one or more electric fields that assist loading the sample into the reaction chamber. The apparatus may further include a sample reservoir having a fluid seal with the surface of the integrated device and configured to hold a suspension containing the samples.

公开(公告)号：US11932906B2

公开(公告)日：2024-03-19

申请号：US17350509

申请日：2021-06-17

Applicant: Quantum-Si Incorporated

Inventor： Guojun Chen ,  Jeremy Lackey ,  Alexander Goryaynov ,  Gerard Schmid ,  Ali Kabiri ,  Jonathan M. Rothberg ,  Todd Rearick ,  Jonathan C. Schultz ,  Farshid Ghasemi ,  Keith G. Fife

IPC: C12Q1/6869 ,  B01L3/00 ,  C08L1/02 ,  C12Q1/6806 ,  G01N21/64 ,  G01N27/447 ,  G01N30/86 ,  G01N30/88

CPC classification number: C12Q1/6869 ,  B01L3/502761 ,  B01L3/50857 ,  C08L1/02 ,  C12Q1/6806 ,  G01N21/6428 ,  G01N21/6454 ,  G01N27/44743 ,  G01N27/44782 ,  G01N30/86 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2400/0415 ,  B01L2400/0421 ,  G01N27/447 ,  G01N30/88

Abstract: Apparatus and techniques for electrokinetic loading of samples of interest into sub-micron-scale reaction chambers are described. Embodiments include an integrated device and related apparatus for analyzing samples in parallel. The integrated device may include at least one reaction chamber formed through a surface of the integrated device and configured to receive a sample of interest, such as a molecule of nucleic acid. The integrated device may further include electrodes patterned adjacent to the reaction chamber that produce one or more electric fields that assist loading the sample into the reaction chamber. The apparatus may further include a sample reservoir having a fluid seal with the surface of the integrated device and configured to hold a suspension containing the samples.

公开(公告)号：US20220305487A1

公开(公告)日：2022-09-29

申请号：US17545127

申请日：2021-12-08

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Jeremy Lackey ,  Guojun Chen

IPC: B01L3/00 ,  C12Q1/6848 ,  C09D5/16 ,  C09D187/00 ,  C09D171/02

Abstract: Methods of preparing surfaces of sample wells are provided. In some aspects, methods of preparing a sample well surface involve contacting the sample well with a block copolymer to form an antifouling overlay over a metal oxide surface of the sample well. In some aspects, methods of passivating and/or selectively functionalizing a sample well surface are provided.

公开(公告)号：US20200171484A1

公开(公告)日：2020-06-04

申请号：US16580877

申请日：2019-09-24

Applicant: Quantum-Si Incorporated

Inventor： Guojun Chen ,  James Beach ,  Kathren Fink ,  Jeremy Lackey ,  Gerard Schmid

IPC: B01L3/00 ,  G02B6/136 ,  G02B6/132

Abstract: A method of forming an integrated device includes forming a sample well within a cladding layer of a substrate; forming a sacrificial spacer layer over the substrate and into the sample well; performing a directional etch of the sacrificial spacer layer so as to form a sacrificial sidewall spacer on sidewalls of the sample well; forming, over the substrate and into the sample well, a functional layer that provides a location for attachment of a biomolecule; and removing the sacrificial spacer material.

公开(公告)号：US20240157395A1

公开(公告)日：2024-05-16

申请号：US18333880

申请日：2023-06-13

Applicant: Quantum-Si Incorporated

Inventor： Guojun Chen ,  Jeremy Lackey

IPC: B05D1/00 ,  B05D3/10 ,  G01N33/543

CPC classification number: B05D1/60 ,  B05D3/104 ,  G01N33/54306 ,  G01N33/54366 ,  B05D2203/30 ,  B05D2350/60

Abstract: Aspects of the application provide methods of producing substrates having modified surfaces. In some aspects, methods of surface modification involve treating a surface of a substrate with an organic reagent in vapor phase to form an organic layer over the surface. In some aspects, methods of forming a stable surface coating on an oxidized surface are provided.

公开(公告)号：US11712715B2

公开(公告)日：2023-08-01

申请号：US17067184

申请日：2020-10-09

Applicant: Quantum-Si Incorporated

Inventor： Guojun Chen ,  Jeremy Lackey

IPC: C23C16/02 ,  C23C16/00 ,  B05D1/00 ,  B05D3/10 ,  G01N33/543

CPC classification number: B05D1/60 ,  B05D3/104 ,  G01N33/54306 ,  G01N33/54366 ,  B05D2203/30 ,  B05D2350/60

Abstract: Aspects of the application provide methods of producing substrates having modified surfaces. In some aspects, methods of surface modification involve treating a surface of a substrate with an organic reagent in vapor phase to form an organic layer over the surface. In some aspects, methods of forming a stable surface coating on an oxidized surface are provided.

公开(公告)号：US20220098658A1

公开(公告)日：2022-03-31

申请号：US17481195

申请日：2021-09-21

Applicant: Quantum-Si Incorporated

Inventor： Guojun Chen ,  Thomas Raymond Thurston ,  An Li

IPC: C12Q1/6869 ,  G01N21/64

Abstract: Provided herein are methods and integrated devices for improved sequencing of nucleic acid and peptide biomolecules. The present disclosure relates to improved mechanisms for protecting a luminescent label from photo-induced damage through the use of quenching moieties. Further provided herein are methods for improved immobilization of quenching moieties and other molecules of interest through functionalization with chemical moieties, such as click chemistry handles, capable of participating in cross-linking reactions. Quenching moieties may be immobilized to the surface of a sample well in a sequencing substrate or apparatus in a manner that minimizes or eliminates photobleaching of the labeled molecule. The disclosed methods provide for minimized photodamage, increased sensitivity, accuracy and length of reads during nucleic acid and polypeptide sequencing.