公开(公告)号：US20220073973A1

公开(公告)日：2022-03-10

申请号：US17314626

申请日：2021-05-07

申请人： Quantum-Si incorporated

发明人： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang

IPC分类号： C12Q1/6818 ,  C12Q1/6869 ,  G01N21/64 ,  C12Q1/6874

摘要： Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.

公开(公告)号：US20210218218A1

公开(公告)日：2021-07-15

申请号：US17148515

申请日：2021-01-13

申请人： Quantum-Si Incorporated

发明人： Michael Bellos ,  Alexander Goryaynov ,  Benjamin Cipriany ,  Xinghua Shi ,  Faisal R. Ahmad

IPC分类号： H01S3/10 ,  H01S3/11 ,  H01S3/106 ,  H01S3/107 ,  G01N21/64

摘要： Systems and methods are described for producing an amplitude-modulated laser pulse train. The laser pulse train can be used to cause fluorescence in materials at which the pulse trains are directed. The parameters of the laser pulse train are selected to increase fluorescence relative to a constant-amplitude laser pulse train. The amplitude-modulated laser pulse trains produced using the teachings of this invention can be used to enable detection of specific molecules in applications such as gene or protein sequencing.

公开(公告)号：US10174363B2

公开(公告)日：2019-01-08

申请号：US15161125

申请日：2016-05-20

申请人： Quantum-Si Incorporated

发明人： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang

IPC分类号： C12Q1/6818 ,  C12Q1/6869 ,  G01N21/64 ,  C12Q1/6874

摘要： Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.

公开(公告)号：US20170362651A1

公开(公告)日：2017-12-21

申请号：US15600979

申请日：2017-05-22

申请人： Quantum-Si Incorporated

发明人： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang ,  David Dodd

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6869 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q2525/101 ,  C12Q2525/197 ,  C12Q2525/301 ,  C12Q2563/103

摘要： Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction. In some aspects, the disclosure provides compositions comprising luminescently labeled nucleotides.

公开(公告)号：US11001875B2

公开(公告)日：2021-05-11

申请号：US16212724

申请日：2018-12-07

申请人： Quantum-Si Incorporated

发明人： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang

IPC分类号： C12Q1/6818 ,  C12Q1/6874 ,  G01N21/64 ,  C12Q1/6869

摘要： Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.

公开(公告)号：US20190330688A1

公开(公告)日：2019-10-31

申请号：US16212724

申请日：2018-12-07

申请人： Quantum-Si Incorporated

发明人： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang

IPC分类号： C12Q1/6818 ,  C12Q1/6869 ,  G01N21/64 ,  C12Q1/6874

摘要： Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.

公开(公告)号：US20240068027A1

公开(公告)日：2024-02-29

申请号：US18188122

申请日：2023-03-22

申请人： Quantum-Si Incorporated

发明人： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang ,  David Dodd

IPC分类号： C12Q1/6876 ,  C07H19/073 ,  C07H19/173 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6816

CPC分类号： C12Q1/6876 ,  C07H19/073 ,  C07H19/173 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6869

摘要： Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleic acid connected to a nucleotide and two or more luminescent labels. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

公开(公告)号：US11655504B2

公开(公告)日：2023-05-23

申请号：US16043547

申请日：2018-07-24

申请人： Quantum-Si Incorporated

发明人： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang ,  David Dodd

IPC分类号： C12Q1/6876 ,  C12Q1/6816 ,  C12Q1/6806 ,  C07H19/073 ,  C07H21/04 ,  C07H19/173 ,  C12Q1/6869

CPC分类号： C12Q1/6876 ,  C07H19/073 ,  C07H19/173 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2525/185 ,  C12Q2525/197 ,  C12Q2525/313 ,  C12Q2563/103 ,  C12Q2600/16 ,  C12Q1/6869 ,  C12Q2525/197 ,  C12Q2563/103 ,  C12Q2565/102 ,  C12Q1/6869 ,  C12Q2525/197 ,  C12Q2563/107 ,  C12Q2565/102

摘要： Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleic acid connected to a nucleotide and two or more luminescent labels. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

公开(公告)号：US11613772B2

公开(公告)日：2023-03-28

申请号：US16751138

申请日：2020-01-23

申请人： Quantum-Si Incorporated

发明人： Jonathan M. Rothberg ,  Jeremy Lackey ,  Brian Reed ,  Xinghua Shi ,  Haidong Huang ,  David Dodd

IPC分类号： C12Q1/68 ,  G01N21/64 ,  C12Q1/6818 ,  C12Q1/6869

摘要： Compositions useful for the detection of single molecules in a sample are provided. In some aspects, the disclosure provides a nucleotide connected to a nucleic acid comprising a FRET label comprising at least three luminescent molecules. In some embodiments, the nucleic acids described herein comprise one or more structural features that provide enhanced fluorescence intensity. In some aspects, methods of sequencing using the labeled nucleotides of the disclosure are provided.

公开(公告)号：US20220128469A1

公开(公告)日：2022-04-28

申请号：US17511249

申请日：2021-10-26

申请人： Quantum-Si Incorporated

发明人： Brian Reed ,  Kyle Preston ,  Xinghua Shi ,  Gerard Schmid

IPC分类号： G01N21/64 ,  G01N33/68

摘要： Aspects of the present disclosure relate to techniques for calibrating a system comprising integrated device. According to some embodiments, there is provided a method for calibrating a system comprising an integrated device, the method comprising: exciting, with light from at least one excitation source, a reference dye molecule disposed in a chamber of the integrated device; obtaining a signal emitted by the reference dye molecule, the signal containing information representative of a bleaching time of the reference dye molecule; and adjusting one or more characteristics of the system based on the bleaching time of the reference dye molecule.