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    Methods of rapid detection and identification of bioagents using microRNA
    4.
    发明申请
    Methods of rapid detection and identification of bioagents using microRNA 审中-公开
    使用microRNA快速检测和鉴定生物标本的方法

    公开(公告)号：US20050123952A1

    公开(公告)日：2005-06-09

    申请号：US10933928

    申请日：2004-09-03

    申请人： Richard Griffey ,  David Ecker

    发明人： Richard Griffey ,  David Ecker

    IPC分类号： C12N20060101 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6888 ,  C12Q1/6816 ,  C12Q2600/156 ,  C12Q2531/113 ,  C12Q2525/15 ,  C12Q2565/627

    摘要： Methods for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of microRNA containing nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of microRNA containing nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a “base composition signature” (BCS) or molecular mass which is then matched against a database of base composition signatures or molecular masses, by which the species of the bioagent is identified.

    摘要翻译： 通过使用与含有衍生自生物制剂的含有核酸的微RNA的保守序列区杂交的引物与包含核酸扩增的微小RNA的组合来检测和识别未知生物标签（包括细菌，病毒等）的方法， 唯一识别生物标签的序列区域。 结果是“基础组成特征”（BCS）或分子量，然后与基础组成特征或分子量的数据库进行匹配，通过该数据库识别出生物代谢物种。

    Microrna as ligands and target molecules
    7.
    发明申请
    Microrna as ligands and target molecules 审中-公开
    Microrna作为配体和靶分子

    公开(公告)号：US20050142581A1

    公开(公告)日：2005-06-30

    申请号：US10934798

    申请日：2004-09-03

    申请人： Richard Griffey ,  C. Bennett ,  David Ecker ,  Donna Ward ,  Susan Freier

    发明人： Richard Griffey ,  C. Bennett ,  David Ecker ,  Donna Ward ,  Susan Freier

    IPC分类号： C12N20060101 ,  C12N15/11 ,  C12N15/113 ,  C12Q1/68

    CPC分类号： C12N15/111 ,  C12N15/1138 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2320/11 ,  C12N2330/10 ,  C12N2310/3525

    摘要： The present invention provides methods for the identification of target molecules that bind to ligands, particularly microRNA ligands and mimics thereof and/or microRNA target molecules and mimics thereof, with as little as millimolar (mM) affinity using mass spectrometry. The methods may be used to determine the mode of binding interaction between two or more of these target molecules to the ligand as well as their relative affinities. Also provided are methods for designing compounds having greater affinity to a ligand by identifying two or more target molecules using mass spectrometry methods of the invention and linking the target molecules together to form a novel compound.

    摘要翻译： 本发明提供用于使用质谱法以少至毫摩尔（mM）亲和力鉴定结合配体，特别是微小RNA配体及其模拟物和/或微小RNA靶分子及其模拟物的靶分子的方法。 该方法可用于确定这些靶分子中两个或更多个与配体之间的结合相互作用的模式以及它们的相对亲和力。 还提供了通过使用本发明的质谱方法鉴定两个或更多个靶分子并将靶分子连接在一起以形成新化合物来设计对配体具有更大亲和力的化合物的方法。