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1.发明授权Method for producing methacrylic acid acrylic acid with a combination of enzyme catalysts 失效用酶催化剂组合生产甲基丙烯酸和丙烯酸的方法公开(公告)号：US06670158B2
公开(公告)日：2003-12-30
申请号：US10067652
申请日：2002-02-05
IPC分类号： C12P1300
CPC分类号： C12P7/40
摘要： The invention provides a process for the hydrolysis of acrylonitrile to acrylic acid, and for the hydrolysis of methacrylonitrile to methacrylic acid, in high yield and at high concentration with high specificity. Acrylonitrile or methacrylonitrile is hydrolyzed in a suitable aqueous reaction mixture by a catalyst characterized by a nitrile hydratase and amidase activity of Comamonas testosteroni 5-MGAM-4D, producing the corresponding acid. The acrylic acid or methacrylic acid is isolated as the acid or corresponding salt.
摘要翻译： 本发明提供了一种将丙烯腈水解成丙烯酸，并以高产率和高浓度高特异性水解甲基丙烯腈至甲基丙烯酸的方法。 丙烯腈或甲基丙烯腈在合适的水性反应混合物中通过催化剂水解，其特征在于产生相应酸的腈水合酶和酰胺酶活性的Comamonas testosteroni 5-MGAM-4D。 分离丙烯酸或甲基丙烯酸作为酸或相应的盐。
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2.发明授权Method for producing &agr;-hydroxy acid from &agr;-hydroxy nitrile using nitrilase 失效使用腈水解酶从α-羟基腈生产α-羟基酸的方法公开(公告)号：US06383786B1
公开(公告)日：2002-05-07
申请号：US09948535
申请日：2001-09-07
IPC分类号： C12P740
摘要： The present invention relates to a method for producing &agr;-hydroxy acids using an enzyme catalyst having nitrilase activity. More specifically, the invention pertains to use of Acidovorax facilis 72W (ATCC 55746) nitrilase to hydrolyze glycolonitrile to glycolic acid. Glycolonitrile is reacted in an aqueous mixture with a catalyst having Acidovorax facilis 72W nitrilase activity to give glycolic acid selectively, and at high concentration and high yield.
摘要翻译： 本发明涉及使用具有腈水解酶活性的酶催化剂生产α-羟基酸的方法。 更具体地，本发明涉及使用Acidovorax facilis 72W（ATCC 55746）腈水解酶将乙醇腈水解成乙醇酸。 乙醇腈在含水混合物中与具有酸性酵母72W腈水解酶活性的催化剂反应，选择性地得到乙醇酸，并以高浓度和高产率。
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3.发明授权公开(公告)号：US06455730B1
公开(公告)日：2002-09-24
申请号：US09632419
申请日：2000-08-04
IPC分类号： C07C5108
摘要： This invention relates to a process for preparing dicarboxylic acid monoesters from cyanocarboxylic acid esters. More particularly, using the nitrilase from Acidovorax facilis 72W (ATCC 55745) in various forms as an enzyme catalyst, an aliphatic or aromatic cyanocarboxylic acid ester is converted to the corresponding dicarboxylic acid monoester with high chemoselectivity at 100% conversion. An embodiment of the invention also provides a process for obtaining high chemoselectively and high regioselectivity.
摘要翻译： 本发明涉及由氰基羧酸酯制备二羧酸单酯的方法。 更具体地，使用来自Acidovorax facilis 72W（ATCC 55745）的腈水解酶以各种形式作为酶催化剂，将脂族或芳族氰基羧酸酯在100％转化率下转化为具有高化学选择性的相应的二羧酸单酯。 本发明的实施方案还提供了获得高化学选择性和高区域选择性的方法。
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4.发明授权Isolation and expression of a gene for a nitrilase from Acidovorax faclilis 72W 失效从酸性酵母72W中分离和表达腈水解酶基因公开(公告)号：US07056709B2
公开(公告)日：2006-06-06
申请号：US10376653
申请日：2003-02-27
IPC分类号： C12P7/40 , C07H21/04 , C12Q1/68 , C07D211/60
CPC分类号： C12P13/00 , C12N9/78 , C12P7/40 , C12P13/002 , C12P13/02
摘要： Recombinant microbial strains are provided that express nitrilase enzyme and are useful as biocatalysts for the hydrolysis of nitrile-containing substrates. The recombinant cells are transformed with a foreign gene isolated from Acidovorax facilis 72W encoding a thermostable nitrilase enzyme that catalyzes the hydrolysis of nitrile-containing substrates to carboxylic acids under mild reaction conditions. The nucleotide sequence of the nitrilase gene and the deduced amino acid sequence encoded by the nitrilase gene are provided.
摘要翻译： 提供重组微生物菌株，其表达腈水解酶，并且可用作用于含腈底物水解的生物催化剂。 将重组细胞用分离自Acidovorax facilis 72W的外源基因转化，其编码热稳定的腈水解酶，其在温和的反应条件下催化含腈底物水解成羧酸。 提供了腈水解酶基因的核苷酸序列和由腈水解酶基因编码的推导的氨基酸序列。
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5.发明授权Isolation and expression of a gene for a nitrilase from Acidovorax facilis 72W 有权从Acidovorax facilis 72W中分离和表达腈水解酶基因公开(公告)号：US06870038B2
公开(公告)日：2005-03-22
申请号：US09823373
申请日：2001-03-30
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N9/78 , C12P7/40 , C12P13/00 , C12P17/10 , C12R1/19 , C07H21/02 , C07H21/04 , C12Q1/68
CPC分类号： C12P13/00 , C12N9/78 , C12P7/40 , C12P13/002 , C12P13/02
摘要： Recombinant microbial strains are provided that express nitrilase enzyme and are useful as biocatalysts for the hydrolysis of nitrile-containing substrates. The recombinant cells are transformed with a foreign gene isolated from Acidovorax facilis 72W encoding a thermostable nitrilase enzyme that catalyzes the hydrolysis of nitrile-containing substrates to carboxylic acids under mild reaction conditions. The nucleotide sequence of the nitrilase gene and the deduced amino acid sequence encoded by the nitrilase gene are provided.
摘要翻译： 提供重组微生物菌株，其表达腈水解酶，并且可用作用于含腈底物水解的生物催化剂。 将重组细胞用分离自Acidovorax facilis 72W的外源基因转化，其编码热稳定的腈水解酶，其在温和的反应条件下催化含腈底物水解成羧酸。 提供了腈水解酶基因的核苷酸序列和由腈水解酶基因编码的推导的氨基酸序列。
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公开(公告)号：US07198926B2
公开(公告)日：2007-04-03
申请号：US10431965
申请日：2003-05-08
申请人： Michael G. Bramucci , Robert Dicosimo , John E. Gavagan , Mark S. Payne , Robert D. Fallon , Sarita Chauhan
发明人： Michael G. Bramucci , Robert Dicosimo , John E. Gavagan , Mark S. Payne , Robert D. Fallon , Sarita Chauhan
IPC分类号： C12P13/00 , C12P7/40 , C12Q1/34 , C12N11/14 , C12N11/02 , C12N1/20 , C12N9/78 , C12N15/00 , C07H21/04 , C07K14/00
CPC分类号： C12N9/88 , C12N9/80 , C12P7/40 , C12P41/006
摘要： A method has been developed to prepare (E)- and (Z)-2-methyl-2-butenoic acids (2M2BA) from a mixture of (E,Z)-2-methyl-2-butenenitriles (2M2BN) by the regioselective hydrolysis of (E)-2M2BN to (E)-2-methyl-2-butenoic acid (2M2BA) using enzyme catalysts having either a nitrilase activity or a combination of nitrile hydratase and amidase activities. The method provides high yields without significant conversion of (Z)-2M2BN to (Z)-2M2BA. The regioselective hydrolysis of (E)-2M2BN to (E)-2M2BA makes possible the facile separation of (E)-2M2BA from (Z)-2M2BN or (Z)-2-methyl-2-butenamide (2M2BAm), and the subsequent conversion of (Z)-2M2BN or (Z)-2M2BAm to (Z)-2M2BA.
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公开(公告)号：US20090036716A1
公开(公告)日：2009-02-05
申请号：US11818353
申请日：2007-06-13
申请人： Michael B. D'Amore , Leo Ernest Manzer , Edward S. Miller, JR. , Robert Dicosimo , Jeffrey P. Knapp
发明人： Michael B. D'Amore , Leo Ernest Manzer , Edward S. Miller, JR. , Robert Dicosimo , Jeffrey P. Knapp
IPC分类号： C07C41/01
摘要： The present invention relates to a catalytic process for making dibutyl ethers using a reactant comprising 1-butanol and water. The dibutyl ethers so produced are useful in transportation fuels.
摘要翻译： 本发明涉及使用包含1-丁醇和水的反应物制备二丁醚的催化方法。 如此生产的二丁醚可用于运输燃料。
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公开(公告)号：US20090023959A1
公开(公告)日：2009-01-22
申请号：US11818358
申请日：2007-06-13
申请人： Michael B. D'Amore , Leo Ernest Manzer , Edward S. Miller, JR. , Robert Dicosimo , Jeffrey P. Knapp
发明人： Michael B. D'Amore , Leo Ernest Manzer , Edward S. Miller, JR. , Robert Dicosimo , Jeffrey P. Knapp
IPC分类号： C07C41/09
摘要： The present invention relates to a process for making dibutyl ethers using dry 1-butanol derived from fermentation broth. The dibutyl ethers so produced are useful in transportation fuels.
摘要翻译： 本发明涉及使用来自发酵液的干燥的1-丁醇制备二丁醚的方法。 如此生产的二丁醚可用于运输燃料。
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公开(公告)号：US20100168237A1
公开(公告)日：2010-07-01
申请号：US12722309
申请日：2010-03-11
CPC分类号： A01N37/02 , A01N37/16 , A01N37/36 , A01N59/00 , A61L2/186 , C12P7/40 , C12P7/62 , A01N63/02 , A01N2300/00
摘要： A process is provided for producing peroxycarboxylic acids from carboxylic acid esters. More specifically, carboxylic acid esters are reacted with an inorganic peroxide, such as hydrogen peroxide, in the presence of an enzyme catalyst having perhydrolysis activity. The present perhydrolase catalysts are classified as members of the carbohydrate esterase family 7 (CE-7) based on the conserved structural features. Further, disinfectant formulations comprising the peracids produced by the processes described herein are provided.
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公开(公告)号：US07091011B2
公开(公告)日：2006-08-15
申请号：US10378202
申请日：2003-03-03
申请人： Michael G. Bramucci , Robert Dicosimo , Robert Fallon , John E. Gavagan , Frank Herkes , Lech Wilczek
发明人： Michael G. Bramucci , Robert Dicosimo , Robert Fallon , John E. Gavagan , Frank Herkes , Lech Wilczek
摘要： This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzyme catalyst 1) nitrile hydratase activity and amidase activity or 2) nitrilase activity of a microbial cell. 3-Hydroxyvaleric acid is used as a substitute for ε-caprolactone in the preparation of highly branched copolyester.
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