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    Laminin 5, 13 and 14 and uses thereof
    4.
    发明授权
    Laminin 5, 13 and 14 and uses thereof 失效
    层粘连蛋白5,13和14及其用途

    公开(公告)号：US06693169B1

    公开(公告)日：2004-02-17

    申请号：US09415625

    申请日：1999-10-12

    申请人： William J. Brunken ,  Richard R. Libby ,  Dale D. Hunter ,  Robert E. Burgeson

    发明人： William J. Brunken ,  Richard R. Libby ,  Dale D. Hunter ,  Robert E. Burgeson

    IPC分类号： C07K1478

    CPC分类号： C07K14/78

    摘要： The present invention features two members of laminin family, i.e., laminin 13 and laminin 14, the methods of making these molecules, and the methods of using these molecules in treating neural disorders, e.g., retinal disorders. The present invention also features using a preparation of laminin 5 to treat neural disorders, especially disorders associated with retina.

    摘要翻译： 本发明具有层粘连蛋白家族的两个成员，即层粘连蛋白13和层粘连蛋白14，制备这些分子的方法，以及使用这些分子治疗神经障碍如视网膜疾病的方法。 本发明还特征在于使用层粘连蛋白5的制剂来治疗神经障碍，特别是与视网膜相关的疾病。

    Products and methods for improving keratinocyte adhesion to the dermis
    6.
    发明授权
    Products and methods for improving keratinocyte adhesion to the dermis 失效
    改善角质细胞对真皮粘附的产品和方法

    公开(公告)号：US5770562A

    公开(公告)日：1998-06-23

    申请号：US469500

    申请日：1995-06-06

    申请人： Robert E. Burgeson ,  Gregory P. Lunstrum ,  Patricia Rousselle ,  Douglas R. Keene ,  M. Peter Marinkovich

    发明人： Robert E. Burgeson ,  Gregory P. Lunstrum ,  Patricia Rousselle ,  Douglas R. Keene ,  M. Peter Marinkovich

    IPC分类号： A61K38/39 ,  A61L27/22 ,  A61L27/60 ,  C12N5/00 ,  C12N5/071 ,  C07K14/78

    CPC分类号： C12N5/0629 ,  A61K38/39 ,  A61L27/227 ,  A61L27/60 ,  C12N5/0068 ,  C12N2501/58 ,  C12N2533/50 ,  C12N2533/52 ,  Y10S530/842

    摘要： The isolated proteins kalinin and k-laminin are disclosed to provide adhesion between epidermal keratinocytes and the underlying dermis. Purified kalinin localizes to the anchoring filaments of basement membranes of human subepithelial skin, trachea, esophagus, cornea and amnion when such areas are probed with BM165 monoclonal antibody after localization. The protein has a molecular weight of approximately 410-495 kDa and exists in a cell-associated form (about 495 kDa) and two medium-associated forms (about 460 and 410 kDa, respectively). The BM165 epitope is located on the 165-kDa and 200 kDa subunits. Kalinin has a rotary-shadow image revealing an asymmetric rod 107-nm long having two globules at a first end and a single globule at an opposing end. The k-laminin adhesion molecule is an isolated heterotrimeric laminin variant that has a molecular weight of about 650 kDa and separates on western blots into first and second fragments that are similar to the B1 and B2 fragments of laminin. K-laminin also includes a third fragment of 190 kDa that is not immunoreactive with monoclonal antibodies 1F5, 11D5 and 4C7, but is immunoreactive with a monoclonal antibody BM165. Kalinin and k-laminin are preferably covalently associated. A method is also disclosed for improving adhesion of transplanted keratinocytes to an underlying substrate, such as the human dermis, by optimizing production of kalinin from cultured keratinocytes, or by providing an exogenous source of kalinin between the keratinocytes and substrate.

    摘要翻译： 公开了分离的蛋白质kalinin和k-层粘连蛋白以提供表皮角化细胞和潜在的真皮之间的粘附。 纯化的卡林林定位于人类上皮皮肤，气管，食管，角膜和羊膜的基底膜的锚定细丝上，当这些区域在定位后用BM165单克隆抗体探测时。 蛋白质具有约410-495kDa的分子量，并以细胞相关形式（约495kDa）和两种培养基相关形式（分别为约460和410kDa）存在。 BM165表位位于165kDa和200kDa亚基上。 加里宁具有旋转阴影图像，其显示107nm长的不对称杆，在第一端具有两个球，在相对端具有单个球。 k-层粘连蛋白粘附分子是分离的异三聚体层粘连蛋白变体，其分子量为约650kDa，并将蛋白质印迹分离成与层粘连蛋白的B1和B2片段相似的第一和第二片段。 K-层粘连蛋白还包括与单克隆抗体1F5,11D5和4C7不具有免疫反应性的190kDa的第三片段，但是与单克隆抗体BM165具有免疫反应性。 卡利宁和k-层粘连蛋白优选共价结合。 还公开了通过优化来自培养的角质形成细胞的生产kalinin或通过在角质形成细胞和底物之间提供外源的卡林林来改善移植的角质形成细胞与底物（例如人真皮）的粘附的方法。