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    Products and methods for improving keratinocyte adhesion to the dermis
    1.
    发明授权
    Products and methods for improving keratinocyte adhesion to the dermis 失效
    改善角质细胞对真皮粘附的产品和方法

    公开(公告)号：US5770562A

    公开(公告)日：1998-06-23

    申请号：US469500

    申请日：1995-06-06

    申请人： Robert E. Burgeson ,  Gregory P. Lunstrum ,  Patricia Rousselle ,  Douglas R. Keene ,  M. Peter Marinkovich

    发明人： Robert E. Burgeson ,  Gregory P. Lunstrum ,  Patricia Rousselle ,  Douglas R. Keene ,  M. Peter Marinkovich

    IPC分类号： A61K38/39 ,  A61L27/22 ,  A61L27/60 ,  C12N5/00 ,  C12N5/071 ,  C07K14/78

    CPC分类号： C12N5/0629 ,  A61K38/39 ,  A61L27/227 ,  A61L27/60 ,  C12N5/0068 ,  C12N2501/58 ,  C12N2533/50 ,  C12N2533/52 ,  Y10S530/842

    摘要： The isolated proteins kalinin and k-laminin are disclosed to provide adhesion between epidermal keratinocytes and the underlying dermis. Purified kalinin localizes to the anchoring filaments of basement membranes of human subepithelial skin, trachea, esophagus, cornea and amnion when such areas are probed with BM165 monoclonal antibody after localization. The protein has a molecular weight of approximately 410-495 kDa and exists in a cell-associated form (about 495 kDa) and two medium-associated forms (about 460 and 410 kDa, respectively). The BM165 epitope is located on the 165-kDa and 200 kDa subunits. Kalinin has a rotary-shadow image revealing an asymmetric rod 107-nm long having two globules at a first end and a single globule at an opposing end. The k-laminin adhesion molecule is an isolated heterotrimeric laminin variant that has a molecular weight of about 650 kDa and separates on western blots into first and second fragments that are similar to the B1 and B2 fragments of laminin. K-laminin also includes a third fragment of 190 kDa that is not immunoreactive with monoclonal antibodies 1F5, 11D5 and 4C7, but is immunoreactive with a monoclonal antibody BM165. Kalinin and k-laminin are preferably covalently associated. A method is also disclosed for improving adhesion of transplanted keratinocytes to an underlying substrate, such as the human dermis, by optimizing production of kalinin from cultured keratinocytes, or by providing an exogenous source of kalinin between the keratinocytes and substrate.

    摘要翻译： 公开了分离的蛋白质kalinin和k-层粘连蛋白以提供表皮角化细胞和潜在的真皮之间的粘附。 纯化的卡林林定位于人类上皮皮肤，气管，食管，角膜和羊膜的基底膜的锚定细丝上，当这些区域在定位后用BM165单克隆抗体探测时。 蛋白质具有约410-495kDa的分子量，并以细胞相关形式（约495kDa）和两种培养基相关形式（分别为约460和410kDa）存在。 BM165表位位于165kDa和200kDa亚基上。 加里宁具有旋转阴影图像，其显示107nm长的不对称杆，在第一端具有两个球，在相对端具有单个球。 k-层粘连蛋白粘附分子是分离的异三聚体层粘连蛋白变体，其分子量为约650kDa，并将蛋白质印迹分离成与层粘连蛋白的B1和B2片段相似的第一和第二片段。 K-层粘连蛋白还包括与单克隆抗体1F5,11D5和4C7不具有免疫反应性的190kDa的第三片段，但是与单克隆抗体BM165具有免疫反应性。 卡利宁和k-层粘连蛋白优选共价结合。 还公开了通过优化来自培养的角质形成细胞的生产kalinin或通过在角质形成细胞和底物之间提供外源的卡林林来改善移植的角质形成细胞与底物（例如人真皮）的粘附的方法。

    Product and method for improving keratinocyte adhesion to the dermis
    2.
    发明授权
    Product and method for improving keratinocyte adhesion to the dermis 失效
    改善角质形成细胞对真皮粘附的产物和方法

    公开(公告)号：US5352668A

    公开(公告)日：1994-10-04

    申请号：US936850

    申请日：1992-08-28

    申请人： Robnert E. Burgeson ,  Gregory P. Lunstrum ,  Patricia Rousselle ,  Douglas R. Keene ,  M. Peter Marinkovich

    发明人： Robnert E. Burgeson ,  Gregory P. Lunstrum ,  Patricia Rousselle ,  Douglas R. Keene ,  M. Peter Marinkovich

    IPC分类号： A61K38/17 ,  A61K35/12 ,  A61K35/36 ,  A61K38/39 ,  A61L27/22 ,  A61L27/60 ,  A61P17/00 ,  C07K1/22 ,  C07K14/47 ,  C07K14/78 ,  C07K16/00 ,  C07K16/18 ,  C12N5/00 ,  C12N5/071 ,  A61F2/10 ,  A61K37/12 ,  C07K15/06 ,  C07K15/20

    CPC分类号： C12N5/0629 ,  A61K35/36 ,  A61K38/39 ,  A61L27/227 ,  A61L27/60 ,  C07K14/47 ,  C07K14/78 ,  C07K16/18 ,  C12N5/0068 ,  A61K35/12 ,  C12N2501/58 ,  C12N2533/50 ,  C12N2533/52 ,  Y10S530/842 ,  Y10S530/851 ,  Y10S623/915

    摘要： A purified protein kalinin is disclosed that provides adhesion between epidermal keratinocytes and the underlying dermis. Purified kalinin localizes to the anchoring filaments of basement membranes or human subepithelial skin, trachea, esophagus, cornea and amnion when such areas are probed with BM165 monoclonal antibody after localization. The protein has a molecular weight of approximately 400-460 kDa and exists in a cell-associated form (about 460 kDa) and two medium-associated forms (about 440 and 400 kDa, respectively). The cell-associated form comprises a 200-, a 155- and a 140-kDa subunit, all normally held together by disulflde bonds. The cell-associated form is subjected to extracellular processing to produce the two medium-associated forms, wherein, in the 440-kDa form, the 200-kDa subunit has been processed to a 165-kDa subunit and, in the 400-kDa form, the 155-kDa subunit has been processed to a 105-KDa subunit. The BM165 epitope is located on the 165-kDa subunit. Kalinin has a rotary-shadow image revealing an asymmetric rod 107-nm long having two globules at a first end and a single globule at an opposing end. A method is also disclosed for improving adhesion of transplanted keratinocytes to an underlying substrate, such as the human dermis, by optimizing production of kalinin from cultured keratinocytes, or by providing an exogenous source of kalinin between the keratinocytes and substrate.

    摘要翻译： 公开了提供表皮角化细胞和潜在真皮之间的粘附的纯化的蛋白质卡林林。 纯化的卡林林定位于基底膜或人类上皮皮肤，气管，食道，角膜和羊膜的锚定细丝上，当这些区域在定位后用BM165单克隆抗体探测时。 该蛋白质具有约400-460kDa的分子量，并以细胞相关形式（约460kDa）和两种培养基相关形式（分别为约440和400kDa）存在。 细胞相关形式包括通常由二硫键连接在一起的200个，一个155和一个140kDa的亚基。 将细胞相关形式进行细胞外加工以产生两种中等相关形式，其中在440-kDa形式中，200kDa亚基已经被加工成165-kDa亚基，并且以400-kDa形式 ，155-kDa亚基已被处理成105-KDa亚基。 BM165表位位于165-kDa亚基上。 加里宁具有旋转阴影图像，其显示107nm长的不对称杆，在第一端具有两个球，在相对端具有单个球。 还公开了通过优化来自培养的角质形成细胞的生产kalinin或通过在角质形成细胞和底物之间提供外源的卡林林来改善移植的角质形成细胞与底物（例如人真皮）的粘附的方法。

    Collagen XXII, a novel human collagen and uses thereof
    4.
    发明授权
    Collagen XXII, a novel human collagen and uses thereof 失效
    胶原蛋白XXII，一种新型人胶原蛋白及其用途

    公开(公告)号：US07452684B2

    公开(公告)日：2008-11-18

    申请号：US11584250

    申请日：2006-10-20

    申请人： Robert Eugene Burgeson ,  Manuel Koch ,  Douglas R. Keene ,  William Joseph Brunken ,  Leena Bruckner-Tuderman

    发明人： Robert Eugene Burgeson ,  Manuel Koch ,  Douglas R. Keene ,  William Joseph Brunken ,  Leena Bruckner-Tuderman

    IPC分类号： G01N33/53 ,  A61K38/39 ,  A61K38/03 ,  A61K38/08 ,  A61K38/10 ,  A61K38/02 ,  C07K16/00

    CPC分类号： C07K14/78

    摘要： The invention provides isolated nucleic acids molecules, designated collagen XXII nucleic acid molecules, which encode a novel collagen. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing collagen XXII nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a collagen XXII gene has been introduced or disrupted. The invention still further provides isolated collagen XXII proteins, fusion proteins, antigenic-peptides and anti-collagen XXII antibodies. Diagnostic methods utilizing compositions of the invention are also provided.

    摘要翻译： 本发明提供编码新型胶原的分离的核酸分子，指定的胶原XXII核酸分子。 本发明还提供了反义核酸分子，含有胶原XXII核酸分子的重组表达载体，其中引入了表达载体的宿主细胞，以及引入或破坏了胶原XXII基因的非人转基因动物。 本发明还提供了分离的胶原蛋白XXII蛋白，融合蛋白，抗原肽和抗胶原XXII抗体。 还提供了利用本发明组合物的诊断方法。