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    Nucleic acid molecules encoding 10-deacetylbaccatin III-O-acetyl transferase and related products
    5.
    发明授权
    Nucleic acid molecules encoding 10-deacetylbaccatin III-O-acetyl transferase and related products 失效
    编码10-脱乙酰基浆果赤霉素III-O-乙酰转移酶及相关产品的核酸分子

    公开(公告)号：US06818755B2

    公开(公告)日：2004-11-16

    申请号：US09866570

    申请日：2001-05-25

    申请人： Rodney B. Croteau ,  Kevin D. Walker ,  Anne Schoendorf ,  Mark R. Wildung

    发明人： Rodney B. Croteau ,  Kevin D. Walker ,  Anne Schoendorf ,  Mark R. Wildung

    IPC分类号： C07H2104

    CPC分类号： C12P17/02 ,  C12N9/1029

    摘要： Transacylase enzymes of Taxus cuspidata and the use of such enzymes to produce Taxol™, related taxoids, as well as intermediates in the Taxol™ biosynthetic pathway are disclosed. Examples of specific enzymes described herein include taxadienol 5-O-acetyl transacylase (TAX1) and 10-deacetylbaccatin III-10-O-acetyl transferase (TAX6). Also disclosed are nucleic acid sequences encoding the T. cuspidata transacylase enzymes.

    摘要翻译： 公开了红豆杉的转酰胺酶和使用这些酶来生产Taxol TM，相关紫杉烷类以及Taxol TM生物合成途径中的中间体。 本文所述的特定酶的实例包括Taxadienol 5-O-乙酰基转酰酶（TAX1）和10-脱乙酰基浆果赤霉素III-10-O-乙酰转移酶（TAX6）。 还公开了编码尖锐镰刀菌转酰酶的核酸序列。

    P450 oxygenases and methods of use
    7.
    发明授权
    P450 oxygenases and methods of use 有权
    P450加氧酶及使用方法

    公开(公告)号：US07402417B2

    公开(公告)日：2008-07-22

    申请号：US10565233

    申请日：2004-07-21

    申请人： Rodney B. Croteau ,  Robert Long ,  Stefan Jennewein

    发明人： Rodney B. Croteau ,  Robert Long ,  Stefan Jennewein

    IPC分类号： C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C12P7/66 ,  C12P21/04 ,  C12Q1/26 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/00

    CPC分类号： C12N9/0069

    摘要： This disclosure provides the nucleic acid and protein sequences of novel P450 oxygenases that utilize, at least, taxoid substrates, including taxadiene isomers. The disclosed oxygenases, such as a taxoid 5α-hydroxylase, hydroxylate, at least, the C5 position of a taxoid. Also provided are methods of introducing oxygen at the C5 position of a taxoid and methods of producing the anti-cancer drug, paclitaxel (also known as Taxol™), and other taxoids, such as paclitaxel intermediates.

    摘要翻译： 本公开提供使用至少紫杉烷底物（包括紫杉二烯异构体）的新型P450加氧酶的核酸和蛋白质序列。 公开的加氧酶，例​​如紫杉烷类5α-羟化酶，羟基化物，至少是紫杉烷的C5位置。 还提供了在紫杉烷的C5位置引入氧的方法和制备抗癌药物紫杉醇（也称为Taxol TM）以及其他类紫杉醇如紫杉醇中间体的方法。

    Nucleic acids encoding Taxus geranylgeranyl diphosphate synthase, and methods of use
    8.
    发明授权
    Nucleic acids encoding Taxus geranylgeranyl diphosphate synthase, and methods of use 有权
    编码红豆杉香叶基二磷酸合成酶的核酸及其使用方法

    公开(公告)号：US6043072A

    公开(公告)日：2000-03-28

    申请号：US187050

    申请日：1998-11-05

    申请人： Rodney B. Croteau ,  Jerry L. Hefner

    发明人： Rodney B. Croteau ,  Jerry L. Hefner

    IPC分类号： C12N15/09 ,  C12N5/10 ,  C12N9/10 ,  C12N15/82 ,  C12R1/91 ,  C07H21/04 ,  C07K1/00 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12N9/1085 ,  C12N15/8243 ,  C12Y205/01029

    摘要： A cDNA encoding geranylgeranyl diphosphate synthase from Canadian Yew (Taxus canadensis) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of geranylgeranyl diphosphate synthase (SEQ ID NO:2), from Canadian Yew (Taxus canadensis). In other aspects, replicable recombinant cloning vehicles are provided which code for geranylgeranyl diphosphate synthase, or for a base sequence sufficiently complementary to at least a portion of geranylgeranyl diphosphate synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranylgeranyl diphosphate synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant geranylgeranyl diphosphate synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant geranylgeranyl diphosphate synthase may be used to obtain expression or enhanced expression of geranylgeranyl diphosphate synthase in plants in order to enhance the production of geranylgeranyl diphosphate, or diterpenes derived therefrom, or may be otherwise employed for the regulation or expression of geranylgeranyl diphosphate synthase, or the production of its products.

    摘要翻译： 已经分离并测序了来自加拿大红豆杉（Taxus canadensis）的编码香叶基香叶基二磷酸合成酶的cDNA，并测定了相应的氨基酸序列。 因此，提供编码来自加拿大红豆杉（Taxus canadensis）的香叶基香叶基二磷酸合成酶（SEQ ID NO：2）的表达的分离的DNA序列（SEQ ID NO：1）。 在其它方面，提供编码香叶基香叶基二磷酸合酶的可重复克隆载体，或与至少一部分香叶基香叶基二磷酸合成酶DNA或RNA足够互补的碱基序列，以使其能够进行杂交。 在另一方面，提供已经用编码香叶基香叶基二磷酸合成酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。 因此，提供了用于重组表达上述重组er牛儿基ger牛儿基二磷酸合成酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。 可以使用重组er牛儿基ger牛儿基二磷酸合成酶在植物中获得表达或增强er牛儿基ger牛儿基二磷酸合成酶的表达，以增强er牛儿基ger牛儿基二磷酸酯的产生，或由此衍生的二萜烯的生产，或可用于调节或表达香叶基香叶基二磷酸合成酶，或 其产品的生产。