利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

84 条记录 (耗时 0.059 秒)

    Human liver epithelial cell line and culture media therefor
    3.
    发明授权
    Human liver epithelial cell line and culture media therefor 失效
    人肝上皮细胞系及其培养基

    公开(公告)号：US5529920A

    公开(公告)日：1996-06-25

    申请号：US879165

    申请日：1992-05-01

    申请人： Katharine H. Cole ,  John F. Lechner ,  Roger Reddel ,  Curtis C. Harris ,  Andrea M. Pfeifer

    发明人： Katharine H. Cole ,  John F. Lechner ,  Roger Reddel ,  Curtis C. Harris ,  Andrea M. Pfeifer

    IPC分类号： C12N5/071 ,  C12Q1/04 ,  G01N33/50 ,  C12N5/06 ,  A01N63/00 ,  C12N5/10 ,  C12Q1/02

    CPC分类号： C12N5/067 ,  C12Q1/045 ,  G01N33/5017 ,  C12N2500/14 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2510/04 ,  G01N2333/914

    摘要： The present invention relates to long term multiplication and permanent establishment of a cell line of human liver epithelial cells(hepatocytes). The human liver epithelial cell line is capable of mitotically proliferating and continuously growing in vitro under suitable environmental conditions in suitable culture media. A method of producing an immortalized human liver epithelial cell line is also disclosed. The invention also relates to serum-free cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an effective cell growth promoting amount of calcium ions; an effective cell growth promoting amount of glucose; an effective amount of insulin to aid cells in glucose uptake; an effective cell growth promoting amount of hydrocortisone; an effective amount of epidermal growth factor to bind epidermal growth factor receptors on cells; an effective amount of transferrin to increase DNA synthesis in cells; an effective amount of cholera toxin to increase DNA synthesis in cells; an effective amount of triiodothyronine to increase DNA synthesis in cells; and an effective growth promoting amount of mammalian hormones and mitogenic factors, including lipoprotein, cholesterol, phospholipids and fatty acids.

    摘要翻译： 本发明涉及长期繁殖和永久建立人肝上皮细胞（肝细胞）的细胞系。 人类肝上皮细胞系能够在合适的环境条件下在合适的培养基中体外增殖并连续生长。 还公开了永生化人肝上皮细胞系的制备方法。 本发明还涉及开发用于支持长期繁殖和永久建立人肝上皮细胞细胞系的无血清细胞培养基。 介质可能含有有效的细胞生长促进量的钙离子; 有效的细胞生长促进量的葡萄糖; 有效量的胰岛素以帮助细胞摄取葡萄糖; 有效的细胞生长促进量的氢化可的松; 有效量的表皮生长因子结合细胞上的表皮生长因子受体; 有效量的转铁蛋白增加细胞中的DNA合成; 有效量的霍乱毒素以增加细胞中的DNA合成; 有效量的三碘甲状腺原氨酸增加细胞中的DNA合成; 以及哺乳动物激素和促有丝分裂因子的有效生长促进量，包括脂蛋白，胆固醇，磷脂和脂肪酸。

    Cell culture medium for human liver epithelial cell line
    5.
    发明授权
    Cell culture medium for human liver epithelial cell line 失效
    人肝上皮细胞系细胞培养液

    公开(公告)号：US5342777A

    公开(公告)日：1994-08-30

    申请号：US844873

    申请日：1992-03-03

    申请人： Katharine H. Cole ,  John F. Lechner ,  Curtis C. Harris

    发明人： Katharine H. Cole ,  John F. Lechner ,  Curtis C. Harris

    IPC分类号： C12N5/071 ,  C12Q1/04 ,  G01N33/50 ,  C12N1/00 ,  C12N5/00 ,  C12N15/00 ,  C12Q1/00

    CPC分类号： G01N33/5017 ,  C12N5/067 ,  C12Q1/045 ,  C12N2500/14 ,  C12N2500/20 ,  C12N2500/22 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/84 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2510/04 ,  G01N2333/914 ,  Y10S435/948

    摘要： The present invention relates to cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an effective cell growth promoting amount of calcium ions; an effective cell growth promoting amount of glucose; an effective amount of insulin to aid cells in glucose uptake; an effective cell growth promoting amount of hydrocortisone; an effective amount of epidermal growth factor to bind epidermal growth factor receptors on cells; an effective amount of transferrin to increase DNA synthesis in cells; an effective amount of cholera toxin to increase DNA synthesis in cells; an effective amount of triiodothyronine to increase DNA synthesis in cells; and an effective growth promoting amount of mammalian hormones and mitogenic factors, including lipoprotein, cholesterol, phospholipids and fatty acids.

    摘要翻译： 本发明涉及开发用于支持长期繁殖和永久建立人肝上皮细胞细胞系的细胞培养基。 介质可能含有有效的细胞生长促进量的钙离子; 有效的细胞生长促进量的葡萄糖; 有效量的胰岛素以帮助细胞摄取葡萄糖; 有效的细胞生长促进量的氢化可的松; 有效量的表皮生长因子结合细胞上的表皮生长因子受体; 有效量的转铁蛋白增加细胞中的DNA合成; 有效量的霍乱毒素以增加细胞中的DNA合成; 有效量的三碘甲状腺原氨酸增加细胞中的DNA合成; 以及哺乳动物激素和促有丝分裂因子的有效生长促进量，包括脂蛋白，胆固醇，磷脂和脂肪酸。

    Protoplast fusion method for high-frequency DNA transfection in human cells
    6.
    发明授权
    Protoplast fusion method for high-frequency DNA transfection in human cells 失效
    人体细胞高频DNA转染的原生质体融合方法

    公开(公告)号：US4608339A

    公开(公告)日：1986-08-26

    申请号：US545257

    申请日：1983-10-25

    申请人： George H. Yoakum ,  Curtis C. Harris ,  Brent E. Korba ,  John F. Lechner

    发明人： George H. Yoakum ,  Curtis C. Harris ,  Brent E. Korba ,  John F. Lechner

    IPC分类号： C07K14/02 ,  C12N5/16 ,  C12N15/87 ,  C12N15/00 ,  C12N5/00 ,  C12P21/02

    CPC分类号： C07K14/005 ,  C12N15/87 ,  C12N5/16 ,  C12N2510/00 ,  C12N2730/10122

    摘要： A modified protoplast fusion method and cell line is disclosed that stably transfects human cells with pSV2-derived plasmids at frequencies greater than 10.sup.-3. This procedure makes it possible to test the biological effect of individual genes (i.e., oncogenes and other cellular genes, and viral genes). To demonstrate the utility of this invention, a pSV2gpt.sup.+ plasmid constructed to carry a subgenomic fragment of hepatitis B virus (HBV) that contained the core antigen gene (HBc gene) is transfected into human cells. Human cell lines are stably transfected with the HBC.sup.+ gene by selecting recipient cells for expression of guanine phosphoribosyl transferase expression; other selective markers, i.e., neomycin resistance, can also be used. Conditions for enhancing the expression of the transfected gene(s) have also been developed. For example, with this gpt.sup.+ /HBc.sup.+ cell line it is shown that growth in serum-free medium or treatment with 5'-azacytidine stimulates the production of the HBV core antigen.

    摘要翻译： 公开了修饰的原生质体融合方法和细胞系，其以大于10-3的频率以pSV2衍生的质粒稳定转染人细胞。 该方法可以测试各基因（即癌基因和其他细胞基因以及病毒基因）的生物学效应。 为了证明本发明的实用性，将构建用于携带含有核心抗原基因（HBc基因）的乙型肝炎病毒（HBV）亚基因组片段的pSV2gpt +质粒转染入人细胞。 通过选择用于表达鸟嘌呤磷酸核糖转移酶表达的受体细胞，用HBC +基因稳定转染人细胞系; 也可以使用其他选择标记，即新霉素抗性。 还开发了用于增强转染基因表达的条件。 例如，使用这种gpt + / HBc +细胞系，显示无血清培养基的生长或用5'-氮杂胞苷碱处理刺激HBV核心抗原的产生。