利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

59 条记录 (耗时 0.022 秒)

    Method of quantitative assay for 1,5-anhydroglucitol
    1.
    发明授权
    Method of quantitative assay for 1,5-anhydroglucitol 失效
    1,5-脱水葡萄糖醇的定量测定方法

    公开(公告)号：US06268166B1

    公开(公告)日：2001-07-31

    申请号：US08230440

    申请日：1994-04-19

    申请人： Ryo Kojima ,  Yoshiro Sato ,  Takeshi Nagasawa

    发明人： Ryo Kojima ,  Yoshiro Sato ,  Takeshi Nagasawa

    IPC分类号： C12Q126

    CPC分类号： C12Q1/26 ,  C12P7/02

    摘要： There is provided an enzymatic method of rapid quantitative assay for 1,5-anhydroglucitol which is also applicable to an automated analysis device. The enzymatic method of quantitative assay for 1,5-anhydroglucitol is characterized by using a pyranose oxidase derived from Basidiomycetous fungi No. 52 as a pyranose oxidase. According to the assaying method of the present invention, assay for 1,5-anhydroglucitol in a specimen can be performed using an automated analysis device, results in rapid assay and improved accuracy.

    摘要翻译： 提供了用于1,5-脱水葡萄糖醇的快速定量测定法的酶法，其也适用于自动化分析装置。 1,5-脱水葡萄糖醇的定量测定法的特征在于使用源自Basidiomycetous真菌No.52的吡喃糖氧化酶作为吡喃糖氧化酶。 根据本发明的测定方法，可以使用自动分析装置进行样品中1,5-脱水葡萄糖醇的测定，结果快速测定并提高精度。

    Method of quantitative assay for 1,5-anhydroglucitol
    3.
    发明授权
    Method of quantitative assay for 1,5-anhydroglucitol 失效
    1,5-脱水葡萄糖醇的定量测定方法

    公开(公告)号：US5468621A

    公开(公告)日：1995-11-21

    申请号：US21250

    申请日：1993-02-23

    申请人： Ryo Kojima ,  Yoshiro Sato ,  Takeshi Nagasawa

    发明人： Ryo Kojima ,  Yoshiro Sato ,  Takeshi Nagasawa

    IPC分类号： C12Q1/26 ,  C12Q1/48 ,  C12R1/645 ,  C12Q1/28

    CPC分类号： C12Q1/26 ,  Y10S435/962

    摘要： A quantitative assay for 1,5-anhydroglucitol in a specimen is described. This assay comprises treating a specimen with a phosphorylating enzyme in the presence of 5 to 500 mM adenosine-5'-triphosphate in a pH range of 7.2 to 8.5, to selectively eliminate sugars other than 1,5-anhydroglucitol, and reacting the product of this reaction, without removing the residual ATP, with pyranose oxidase in the above pH range. This assay is quite rapid and suitable for performance using an automated device.

    摘要翻译： 描述了样品中1,5-脱水葡萄糖醇的定量测定。 该测定包括在pH范围为7.2至8.5的5至500mM腺苷-5'-三磷酸的存在下用磷酸化酶处理样品，以选择性除去1,5-脱水葡萄糖醇以外的糖，并使 该反应在上述pH范围内不用吡喃糖氧化酶除去残留的ATP。 该测定非常快速，适用于使用自动化设备的性能。

    Method for determination of direct bilirubin and reagent therefor
    4.
    发明授权
    Method for determination of direct bilirubin and reagent therefor 失效
    测定直接胆红素及其试剂的方法

    公开(公告)号：US5869276A

    公开(公告)日：1999-02-09

    申请号：US90332

    申请日：1998-06-04

    申请人： Ryo Kojima ,  Yoshikiyo Sasagawa ,  Yasunori Okazaki ,  Takeshi Nagasawa

    发明人： Ryo Kojima ,  Yoshikiyo Sasagawa ,  Yasunori Okazaki ,  Takeshi Nagasawa

    IPC分类号： C12Q1/26 ,  C12Q1/00 ,  G01N33/53

    CPC分类号： C12Q1/26 ,  Y10S435/961

    摘要： There is provided a method for determination of direct bilirubin which comprises the steps of contacting a bilirubin oxidase with a sample suspected of containing bilirubin; and, measuring direct bilirubin in the sample by optical changes of the sample, characterized in that bilirubin oxidase is allowed to act in the presence of a indirect bilirubin reaction inhibitor selected from a thiocyanate ion, a hydrazide, reduced nicotinamide adenine dinucleotide, reduced nicotinamide adenine dinucleotide phosphate and a potassium ion of 100 mM to 800 mM. By completely avoiding interference of indirect bilirubin, direct bilirubin is selectively and precisely determined using a reagent kit in the form of solution. The method and reagent of the invention are safe and free of environmental pollution due to unnecessary waste liquid treatment.

    摘要翻译： 提供了用于测定直接胆红素的方法，其包括使胆红素氧化酶与怀疑含有胆红素的样品接触的步骤; 并且通过样品的光学变化测量样品中的直接胆红素，其特征在于允许胆红素氧化酶在选自硫氰酸根离子，酰肼，还原型烟酰胺腺嘌呤二核苷酸，还原型烟酰胺腺嘌呤的间接胆红素反应抑制剂存在下起作用 二磷酸核苷酸和100mM至800mM的钾离子。 通过完全避免间接胆红素的干扰，使用溶液形式的试剂盒选择性和精确地测定直接胆红素。 本发明的方法和试剂是安全的，由于不必要的废液处理而没有环境污染。

    Method for producing oil and fat composition with reduced trans-fatty acid content and processed oil and fat product containing the oil and fat composition
    7.
    发明申请
    Method for producing oil and fat composition with reduced trans-fatty acid content and processed oil and fat product containing the oil and fat composition 审中-公开
    生产具有降低的反式脂肪酸含量的油脂组合物的方法，含有油脂组合物的加工油脂产品

    公开(公告)号：US20070231446A1

    公开(公告)日：2007-10-04

    申请号：US11806222

    申请日：2007-05-30

    申请人： Takeshi Nagasawa ,  Akira Akahane ,  Kaori Muroga ,  Masahiro Arikai

    发明人： Takeshi Nagasawa ,  Akira Akahane ,  Kaori Muroga ,  Masahiro Arikai

    IPC分类号： A23D9/00

    CPC分类号： C12P7/6454 ,  A23D9/00 ,  C11C3/10

    摘要： A method for producing an oil and fat composition includes the steps of (a) mixing an oil and fat (A) wherein fatty acids with 14 or less carbon atoms are contained in an amount of 50 to 100% by mass of the entire constituent fatty acids of the triglycerides with an oil and fat (B) wherein fatty acids with 16 to 24 carbon atoms are contained in an amount of 80 to 100% by mass of the entire constituent fatty acids of the triglycerides to obtain an oil and fat mixture; (b) subjecting the oil and fat mixture to random transesterification using an enzyme having no positional specificity, and (c) obtaining an oil and fat composition free from trans-fatty acid.

    摘要翻译： 油脂组合物的制造方法包括以下步骤：（a）将含有14个以下碳原子的脂肪酸的油脂（A）以50〜100质量％ 甘油三酸酯与油脂（B）的酸，其中含有16至24个碳原子的脂肪酸的量为甘油三酯的全部构成脂肪酸的80至100质量％，以获得油脂混合物; （b）使用不具有位置特异性的酶对油脂混合物进行无规酯交换反应，和（c）获得不含反式脂肪酸的油脂组合物。

    L-argininal acetal derivatives and a process for production thereof
    9.
    发明授权
    L-argininal acetal derivatives and a process for production thereof 失效
    L-精氨酸缩醛衍生物及其制备方法

    公开(公告)号：US5416238A

    公开(公告)日：1995-05-16

    申请号：US755527

    申请日：1991-09-05

    申请人： Yoshihito Abe ,  Akio Hashimoto ,  Takeshi Nagasawa ,  Katsumasa Kuroiwa

    发明人： Yoshihito Abe ,  Akio Hashimoto ,  Takeshi Nagasawa ,  Katsumasa Kuroiwa

    IPC分类号： C07C279/12 ,  B01J31/02 ,  C07B61/00 ,  C07C277/08 ,  C07C279/24 ,  C07C209/78

    CPC分类号： C07C279/12 ,  C07C279/24 ,  Y02P20/55

    摘要： Novel N.sup.G -protected or unprotected-L-argininal-dibutyl acetals (I) can be prepared from L-argininal derivatives (III) merely by reacting in an azeotropic solvent in the presence of p-toluenesulfonic acid. In the products (I), L-form amounts to more than 99%, without further optical resolution. The L-argininal acetals (I) are useful as starting materials for preparing peptidyl-L-argininal derivatives which can provide protease inhibitors or ligands used for affinity chromatography of various proteases.

    摘要翻译： 只有通过在对甲苯磺酸存在下在共沸溶剂中反应，可以由L-精氨酸衍生物（III）制备新的NG-保护的或未受保护的L-精氨酸 - 二丁基缩醛（I）。 在产品（I）中，L型大于99％，无需进一步的光学拆分。 L-精氨酸缩醛（I）可用作制备肽基-L-精氨酸衍生物的起始原料，其可以提供蛋白酶抑制剂或用于各种蛋白酶亲和层析的配体。