公开(公告)号：US5079959A

公开(公告)日：1992-01-14

申请号：US404559

申请日：1989-09-08

申请人： Ryo Miyake ,  Hiroshi Ohki ,  Isao Yamazaki ,  Toshio Kaneko ,  Hideyuki Horiuchi ,  Shinichi Sakuraba ,  Kaori Yasuda

发明人： Ryo Miyake ,  Hiroshi Ohki ,  Isao Yamazaki ,  Toshio Kaneko ,  Hideyuki Horiuchi ,  Shinichi Sakuraba ,  Kaori Yasuda

IPC分类号： G01N15/12 ,  G01N15/14 ,  G01N35/10

CPC分类号： G01N35/1083 ,  G01N15/1404 ,  G01N2035/1086 ,  G01N35/1004

摘要： Particles contained in a liquid sample are analyzed by moving the particles along a detection passage past a detector using a liquid flow in the detection passage. To reduce the time required for analysis of each sample, the sample is delivered at an input location at the upstream end of the detection passage by movement of a movable container carrying the sample to the input location. Immediately after the commencement of the delivery of the liquid sample, liquid flow is caused to occur in the detection passage. Washing liquid is supplied to the input location by a liquid supplier different from the movable container which carries the sample.

公开(公告)号：US5270212A

公开(公告)日：1993-12-14

申请号：US841487

申请日：1992-02-26

申请人： Hideyuki Horiuchi ,  Shinichi Sakuraba ,  Toshio Kaneko ,  Nobuyuki Tatara ,  Ryohei Yabe ,  Hiroshi Ohki ,  Isao Yamazaki ,  Ryo Miyake

发明人： Hideyuki Horiuchi ,  Shinichi Sakuraba ,  Toshio Kaneko ,  Nobuyuki Tatara ,  Ryohei Yabe ,  Hiroshi Ohki ,  Isao Yamazaki ,  Ryo Miyake

IPC分类号： G01N1/00 ,  G01N1/28 ,  G01N15/00 ,  G01N15/14 ,  G01N33/48 ,  G01N35/00

CPC分类号： G01N35/1095 ,  G01N1/28 ,  G01N2015/0069 ,  G01N2015/0076 ,  G01N2015/1409 ,  G01N2021/6439 ,  Y10T436/111666

摘要： This invention relates to an apparatus for analyzing cells in a biological liquid sample, such as blood, in which apparatus a flow passage in a sample pretreatment system can be shortened with a simple construction. A sample metering chamber, a mixing chamber and a measurement liquid extracting chamber are formed in a rotary sliding member of a pretreatment portion. The rotary sliding member is intermittently rotated so as to shift a sample from one chamber to another to perform a necessary treatment. Fluorescence or scattering light with respect to the sample, mixed with a reagent in the pretreatment portion, is detected by a flow cell.

摘要翻译： 本发明涉及用于分析生物液体样品如血液中的细胞的装置，其中可以用简单的结构缩短样品预处理系统中的流动通道。 样品计量室，混合室和测量液体提取室形成在预处理部分的旋转滑动构件中。 旋转滑动构件间歇地旋转，以将样品从一个室移动到另一个室进行必要的处理。 通过流动池检测相对于样品的荧光或散射光与预处理部分中的试剂混合。

公开(公告)号：US5690895A

公开(公告)日：1997-11-25

申请号：US761143

申请日：1996-12-06

申请人： Masaetsu Matsumoto ,  Isao Yamazaki ,  Ryo Miyake ,  Masaharu Ishii ,  Ryohei Yabe ,  Hiroshi Ohki ,  Hideyuki Horiuchi ,  Shinichi Sakuraba

发明人： Masaetsu Matsumoto ,  Isao Yamazaki ,  Ryo Miyake ,  Masaharu Ishii ,  Ryohei Yabe ,  Hiroshi Ohki ,  Hideyuki Horiuchi ,  Shinichi Sakuraba

IPC分类号： G01N15/00 ,  G01N15/02 ,  G01N15/06 ,  G01N15/14 ,  G01N21/05 ,  G01N33/49

CPC分类号： G01N21/05 ,  G01N15/1404 ,  G01N2021/0346

摘要： A flow cell apparatus, useful in an apparatus for measuring particles suspended in a liquid, comprises a sample liquid, supply device, a sheath liquid supply device, a flow cell which has a flow passage passing through a transparent measuring portion, and a nozzle for causing the sample liquid, from the supply device to flow in the flow passage of the flow cell. The nozzle has at least one discharge port which is disposed in the flow passage in a spaced relation from the inner wall of the flow cell flow passage. The sheath liquid supply device causes the sheath liquid to flow around the nozzle discharge port and surround the sample liquid flow to form a sheath flow. The flow passage of the flow cell and the nozzle are so formed as to ensure a sample liquid flow of a fixed width through the measuring portion. A stable sample flow having a fixed width can be formed in the measuring portion even when the flow is at a high velocity, and particles in the sample liquid can efficiently be photographed with high accuracy.

摘要翻译： 一种用于测量悬浮在液体中的颗粒的装置中的流动池装置，包括样品液体，供给装置，皮肤液体供应装置，具有通过透明测量部分的流动通道的流动池，以及用于 使来自供给装置的样品液体流入流动池的流路。 喷嘴具有至少一个排出口，该排出口以与流动池流动通道的内壁隔开的关系设置在流动通道中。 皮肤液体供给装置使皮肤液体在喷嘴排出口周围流动并且围绕样品液体流动形成鞘流。 流动池和喷嘴的流动通道形成为确保通过测量部分具有固定宽度的样品液体流动。 即使在流速高的情况下，也可以在测量部分形成具有固定宽度的稳定的样品流，并且可以高精度地有效地拍摄样品液体中的颗粒。

公开(公告)号：US5880835A

公开(公告)日：1999-03-09

申请号：US703076

申请日：1996-08-26

申请人： Isao Yamazaki ,  Hiroshi Ohki ,  Masaetsu Matsumoto ,  Ryo Miyake ,  Ryohei Yabe ,  Hideyuki Horiuchi ,  Shinichi Sakuraba

发明人： Isao Yamazaki ,  Hiroshi Ohki ,  Masaetsu Matsumoto ,  Ryo Miyake ,  Ryohei Yabe ,  Hideyuki Horiuchi ,  Shinichi Sakuraba

IPC分类号： G01N15/14 ,  G01N15/02 ,  G01N15/06 ,  G01N33/48 ,  G01N33/49 ,  G01N33/493

CPC分类号： G01N15/147 ,  G01N2015/1409 ,  G01N33/49 ,  G01N33/493

摘要： In order to investigate particles in a fluid, a flow cell causes the fluid to flow past a sensor which receives light from a continuous light emission system. When the sensor detects a change in light, a particle detector is triggered, which in turn triggers a pulse generator and hence an intermittent light emission system. Light then illuminates the flow cell to allow a CCD camera to photograph the particle. The photographs taken by the CCD camera are analyzed by an image processor. In order to obtain an accurate particle concentration measurement, it is necessary to modify the initial concentration measurement derived from the analysis of particle images by the image processor by a compensation coefficient. This operation is carried out by a concentration compensator. The result may then be displayed. Additional analysis may be carried out by changing the magnification of the CCD camera, or by selecting for analysis only those particles of a selected size or type.

摘要翻译： 为了研究流体中的颗粒，流动池使得流体流过从连续发光系统接收光的传感器。 当传感器检测到光的变化时，触发粒子检测器，这又触发脉冲发生器，因此触发间歇发光系统。 光然后照亮流动池，以允许CCD照相机拍摄颗粒。 由CCD照相机拍摄的照片由图像处理器分析。 为了获得准确的粒子浓度测量，需要通过图像处理器修正由粒子图像的分析得到的初始浓度测量值的补偿系数。 该操作由浓度补偿器进行。 然后可以显示结果。 可以通过改变CCD照相机的放大倍率或通过选择仅分析选定尺寸或类型的那些颗粒来进行附加分析。

公开(公告)号：US5174162A

公开(公告)日：1992-12-29

申请号：US550543

申请日：1990-07-10

申请人： Ryo Miyake ,  Hiroshi Ohki ,  Hideo Enoki ,  Toshio Kaneko ,  Hideyuki Horiuchi

发明人： Ryo Miyake ,  Hiroshi Ohki ,  Hideo Enoki ,  Toshio Kaneko ,  Hideyuki Horiuchi

IPC分类号： B01F5/06 ,  B01F11/00 ,  B01F13/00 ,  B01L3/02 ,  G01N1/00 ,  G01N21/78 ,  G01N33/543 ,  G01N35/02 ,  G01N35/10

CPC分类号： B01F11/0071 ,  B01F11/0074 ,  B01F13/00 ,  B01F5/0609 ,  B01L3/021 ,  G01N35/10 ,  G01N2035/1058

摘要： A pipetter capable of mixing liquids and dispensing the mixed liquid has a pipetting tube, an actuating device connected to the tube for sucking liquid into and discharging liquid from the tube, and a flow disturbance device in the tube spaced from the discharge and suction end thereof and adapted to cause longitudinal mixing of liquid in the tube when liquid is moved along the tube in at least one direction. Mixing is effected in the tube by causing a liquid body to move repeatedly to and fro along the tube so that during each such movement at least a portion of the liquid body passes the flow disturbance device and the liquid body is not discharged from the first end of the tube. The pipetter is useful for example in mixing a suspension of blood cells with a reagent prior to analysis.

摘要翻译： 能够混合液体并分配混合液体的移液器具有移液管，连接到管的致动装置，用于将液体从管吸入和排出液体，以及与排出和吸入端隔开的管中的流动扰动装置 并且适于当液体沿着至少一个方向移动时引起液体在管中的纵向混合。 通过使液体沿着管道来回重复地在管中进行混合，使得在每次这样的移动期间，液体的至少一部分通过流动扰动装置，液体不从第一端排出 的管。 移液器可用于例如在分析之前将血细胞悬浮液与试剂混合。

公开(公告)号：US5166537A

公开(公告)日：1992-11-24

申请号：US628018

申请日：1990-12-17

申请人： Hideyuki Horiuchi ,  Hiroshi Ohki ,  Toshio Kaneko

发明人： Hideyuki Horiuchi ,  Hiroshi Ohki ,  Toshio Kaneko

IPC分类号： G01N33/49 ,  G01N15/00 ,  G01N15/10 ,  G01N15/12 ,  G01N15/14

CPC分类号： G01N15/1227 ,  G01N2015/0073 ,  G01N2015/1062 ,  G01N2015/1087

摘要： According to the present invention, the accuracy of particle size histograms produced from data relating to an impedance variation (Coutler Counter Method) caused by particles passing between electrodes disposed on either side of an orifice through which the particles pass is improved by excluding false data from the data used in producing the histograms, the false data resulting from more than one particle being resident in the orifice at a given data collection time. The preferred embodiment employs a light detecting method and apparatus to detect false readings and to provide correction information used to correct data obtained by the Coulter Counter Method and apparatus.

公开(公告)号：US5715182A

公开(公告)日：1998-02-03

申请号：US286257

申请日：1994-08-08

申请人： Hideki Asai ,  Hideyuki Horiuchi ,  Ryohei Yabe ,  Norio Oowada

发明人： Hideki Asai ,  Hideyuki Horiuchi ,  Ryohei Yabe ,  Norio Oowada

IPC分类号： G01N15/14 ,  G06F19/00

CPC分类号： G01N15/14 ,  G01N2201/1296 ,  Y10S706/924

摘要： A particle image in a sample is formed at an imaging position by an objective lens of a microscope, projected on the image picking up plane of a TV camera via a projection lens and is subjected to photo-electric conversion. Image signals from the TV camera are supplied to an image memory via an A/D converter as well as to an image processing control unit. Image signals outputted from the image memory are supplied to a characteristic picking out unit and there a plurality of characteristics of the particle concerned are picked out. The picked-out characteristics are supplied to the classification unit and there classification of the sediment components is perfumed via a neural network with a learning capability. Accordingly, the classification unit performs provisionally an automatic classification of the objective sediment components by making use of the inputted characteristic parameters. The device allows accurate and fast automatic component particle analysis even for patient specimens containing a variety of components in high concentration.

摘要翻译： 通过显微镜的物镜在成像位置形成样品中的粒子图像，通过投影透镜投影在电视摄像机的图像拾取面上，并进行光电转换。 来自TV摄像机的图像信号经由A / D转换器以及图像处理控制单元提供给图像存储器。 从图像存储器输出的图像信号被提供给特征选择单元，并且拾取有关粒子的多个特征。 所选择的特征被提供给分类单元，并且通过具有学习能力的神经网络对沉积物成分进行分类。 因此，分类单元通过使用输入的特征参数临时执行目标沉积物成分的自动分类。 该装置允许准确和快速的自动组分颗粒分析，即使对于含有高浓度各种组分的患者标本也是如此。

公开(公告)号：US4175859A

公开(公告)日：1979-11-27

申请号：US815824

申请日：1977-07-14

申请人： Akihide Hashizume ,  Ryuichi Suzuki ,  Hisatake Yokouchi ,  Hideyuki Horiuchi ,  Shinji Yamamoto

发明人： Akihide Hashizume ,  Ryuichi Suzuki ,  Hisatake Yokouchi ,  Hideyuki Horiuchi ,  Shinji Yamamoto

IPC分类号： G01N33/48 ,  G01N15/14 ,  G01N21/25 ,  G06K9/00 ,  G06T1/00 ,  G01N33/16

CPC分类号： G01N15/1468 ,  G06K9/00127 ,  G01N21/25

摘要： An apparatus for automated classification of white blood cells comprising photo-electric converter means whereby the green and blue lights passing through the blood smear are converted into the respective electric signals, a first means for detecting the zone where the nucleus of white blood cell is present at a certain threshold level determined by the maximum and minimum values of the electric signal corresponding to green color, a second means for discriminating between the nucleus of white blood cell obtained from said first means and the granules and cytoplasm of white blood cell at a certain threshold level determined according to said electric signal corresponding to green color, a third means for discriminating between said cytoplasm and the back-ground at the threshold value determined on the basis of said electric signal corresponding to green color and obtained by adding a certain value to the average density on the back-ground, and a fourth means for discriminating between said nucleus and the red blood cells at a certain threshold level determined on the basis of said electric signal corresponding to blue color.

摘要翻译： 一种用于白细胞自动分类的装置，包括光电转换器装置，由此将穿过血液涂片的绿色和蓝色光转换成相应的电信号，第一种用于检测存在白细胞核的区域的装置 在由与绿色对应的电信号的最大值和最小值确定的某个阈值水平上，第二装置，用于鉴别从所述第一装置获得的白细胞的核与特定的白细胞的颗粒和细胞质 根据对应于绿色的所述电信号确定的阈值电平;第三装置，用于基于所述与绿色相对应的电信号确定的阈值来区分所述细胞质和背景，并通过将特定值加到 背景上的平均密度，以及用于区分的第四装置 所述细胞核和红细胞在基于对应于蓝色的所述电信号确定的某个阈值水平。

公开(公告)号：US4129854A

公开(公告)日：1978-12-12

申请号：US844312

申请日：1977-10-21

申请人： Ryuichi Suzuki ,  Akihide Hashizume ,  Hideyuki Horiuchi ,  Shinji Yamamoto ,  Hisatake Yokouchi

发明人： Ryuichi Suzuki ,  Akihide Hashizume ,  Hideyuki Horiuchi ,  Shinji Yamamoto ,  Hisatake Yokouchi

IPC分类号： G06K9/00 ,  G01N33/16

CPC分类号： G06K9/00127

摘要： A method wherein at least one standard leukocyte is selected from within a blood film in advance, the values of adjustments are calculated on the basis of the differences between a plurality of characteristic parameters evaluated from the standard leukocyte and the corresponding characteristic parameters previously given, and the characteristic parameters of the other leukocytes in the particular blood film are adjusted with these values of adjustments. Further, using the adjusted characteristic parameters, secondary characteristics are derived.

摘要翻译： 一种预先从血液膜中选择至少一种标准白细胞的方法，基于从标准白细胞评估的多个特征参数与先前给出的相应特征参数之间的差异来计算调整值，以及 利用这些调整值调整特定血液中的其它白细胞的特征参数。 此外，使用经调整的特征参数，导出次要特性。

公开(公告)号：US5561517A

公开(公告)日：1996-10-01

申请号：US303236

申请日：1994-09-08

申请人： Hideyuki Horiuchi ,  Norio Oowada ,  Hideki Asai ,  Ryohei Yabe

发明人： Hideyuki Horiuchi ,  Norio Oowada ,  Hideki Asai ,  Ryohei Yabe

IPC分类号： G01N15/02 ,  G01N15/14 ,  G01N33/48 ,  G01N33/49

CPC分类号： G01N15/14

摘要： A method and an apparatus for flow type particle image analysis generate a pulse light emission in any one of the odd and the even field image reading-out periods, and multiple exposure is prevented by prohibiting the next pulse light emission in the predetermined period after the emission. The method includes the steps of flowing a sample liquid in a flow cell, detecting particles passing through the flow cell by a laser beam, making a flash lamp emit light after the predetermined period from the particle detection, picking up a particle still image by a TV camera, and further preventing the multiple exposure by providing emission-prohibiting periods which are established by controlling the timings of flash lamp emission and image transfer with the TV camera, a flash lamp emission control circuit, and an image processing control circuit which are controlled by a central control part.

摘要翻译： 用于流式粒子图像分析的方法和装置在奇数和偶数场图像读出周期中的任何一个中产生脉冲发光，并且通过在预定时间段之后禁止下一个脉冲发光来防止多次曝光 排放。 该方法包括以下步骤：将流动池中的样品液体流动，通过激光束检测通过流动池的颗粒，使得闪光灯在从颗粒检测起的预定时间后发光，通过一个 通过提供通过控制闪光灯发射和图像传输的定时而建立的禁止发射周期，通过电视摄像机，闪光灯发射控制电路和图像处理控制电路进行控制，从而进一步防止多次曝光 由中央控制部分。