公开(公告)号：US11807902B2

公开(公告)日：2023-11-07

申请号：US16892488

申请日：2020-06-04

Applicant: STOKES BIO LTD.

Inventor： Mark Davies ,  Tara Dalton

IPC: C12Q1/686 ,  G01N35/08 ,  B01F25/10 ,  B01F33/30 ,  B01L3/00 ,  B01L7/00 ,  G01N35/10 ,  G01N35/00

CPC classification number: C12Q1/686 ,  B01F25/10 ,  B01F33/30 ,  B01L3/50273 ,  B01L3/502776 ,  B01L3/502784 ,  B01L7/525 ,  G01N35/08 ,  B01L2200/0636 ,  B01L2200/0673 ,  B01L2200/141 ,  B01L2300/087 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0487 ,  G01N35/1095 ,  G01N2035/00514

Abstract: A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.

公开(公告)号：US11772096B2

公开(公告)日：2023-10-03

申请号：US16922265

申请日：2020-07-07

Applicant: STOKES BIO LTD.

Inventor： Mark Davies ,  Tara Dalton ,  Kieran Curran

IPC: B01L3/00 ,  B01F13/00 ,  B01F15/02 ,  F17D1/08 ,  C12Q1/686 ,  B01F35/71 ,  B01F33/302 ,  B01F101/23 ,  B01L7/00

CPC classification number: B01L3/502784 ,  B01F33/3021 ,  B01F35/7172 ,  B01L3/502715 ,  B01L3/502746 ,  C12Q1/686 ,  F17D1/08 ,  B01F35/71 ,  B01F2101/23 ,  B01L3/5025 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/0838 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/082 ,  Y10T137/87676 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

Abstract: A system for processing a biological sample includes a substrate comprising a plurality of wells and a plurality of flow channels. The system further includes a flow control system comprising a manifold having a plurality of ports configured to fluidically couple to the plurality of wells, and one or more containment structures configured to contain carrier fluid and fluidically couple to the ports. The flow control system further includes a cradle configured to removably receive the substrate. The flow control system is configured to transmit pressure differential, via the manifold, to the plurality of wells so as to cause a plurality of sample volumes held by at least some wells of the plurality of wells to flow through respective flow channels and cause the carrier fluid to flow through the flow channels and form a plurality of droplets of the plurality of sample volumes separated by the carrier fluid.